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Protein

F-box only protein 9

Gene

FBXO9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • fat cell differentiation Source: Ensembl
  • innate immune response Source: Ensembl
  • protein ubiquitination Source: UniProtKB
  • regulation of TOR signaling Source: UniProtKB
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 9
Alternative name(s):
Cross-immune reaction antigen 1
Renal carcinoma antigen NY-REN-57
Gene namesi
Name:FBXO9
Synonyms:FBX9, VCIA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:13588. FBXO9.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • SCF ubiquitin ligase complex Source: UniProtKB
  • ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28049.

Polymorphism and mutation databases

BioMutaiFBXO9.
DMDMi13124238.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447F-box only protein 9PRO_0000119886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 1361PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UK97.
MaxQBiQ9UK97.
PaxDbiQ9UK97.
PeptideAtlasiQ9UK97.
PRIDEiQ9UK97.

PTM databases

iPTMnetiQ9UK97.
PhosphoSiteiQ9UK97.

Expressioni

Gene expression databases

BgeeiENSG00000112146.
CleanExiHS_FBXO9.
ExpressionAtlasiQ9UK97. baseline and differential.
GenevisibleiQ9UK97. HS.

Organism-specific databases

HPAiHPA064246.

Interactioni

Subunit structurei

Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Interacts with TTI1 and TELO2; when TTI1 and TELO2 are phosphorylated by CK2.1 Publication

Protein-protein interaction databases

BioGridi117652. 9 interactions.
DIPiDIP-60458N.
IntActiQ9UK97. 6 interactions.
STRINGi9606.ENSP00000244426.

Structurei

3D structure databases

ProteinModelPortaliQ9UK97.
SMRiQ9UK97. Positions 186-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati94 – 12734TPRAdd
BLAST
Domaini185 – 23652F-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 TPR repeat.Curated

Keywords - Domaini

TPR repeat

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ9UK97.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG091G0BDF.
PhylomeDBiQ9UK97.
TreeFamiTF324797.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UK97-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPDIIWVFPP QAEAEEDCHS DTVRADDDEE NESPAETDLQ AQLQMFRAQW
60 70 80 90 100
MFELAPGVSS SNLENRPCRA ARGSLQKTSA DTKGKQEQAK EEKARELFLK
110 120 130 140 150
AVEEEQNGAL YEAIKFYRRA MQLVPDIEFK ITYTRSPDGD GVGNSYIEDN
160 170 180 190 200
DDDSKMADLL SYFQQQLTFQ ESVLKLCQPE LESSQIHISV LPMEVLMYIF
210 220 230 240 250
RWVVSSDLDL RSLEQLSLVC RGFYICARDP EIWRLACLKV WGRSCIKLVP
260 270 280 290 300
YTSWREMFLE RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYI
310 320 330 340 350
RFFPDGHVMM LTTPEEPQSI VPRLRTRNTR TDAILLGHYR LSQDTDNQTK
360 370 380 390 400
VFAVITKKKE EKPLDYKYRY FRRVPVQEAD QSFHVGLQLC SSGHQRFNKL
410 420 430 440
IWIHHSCHIT YKSTGETAVS AFEIDKMYTP LFFARVRSYT AFSERPL
Length:447
Mass (Da):52,329
Last modified:May 1, 2000 - v1
Checksum:i2A88163DAB898D69
GO
Isoform 2 (identifier: Q9UK97-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MPDIIWVFPPQ → M

Show »
Length:437
Mass (Da):51,136
Checksum:iAF7DCF6AD4F4035E
GO
Isoform 3 (identifier: Q9UK97-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Note: No experimental confirmation available.
Show »
Length:403
Mass (Da):47,361
Checksum:i1AAA1F46C31315BB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4444Missing in isoform 3. 1 PublicationVSP_012980Add
BLAST
Alternative sequencei1 – 1111MPDIIWVFPPQ → M in isoform 2. 4 PublicationsVSP_012979Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176704 mRNA. Translation: AAF03704.1.
AY858798 mRNA. Translation: AAW51115.1.
AL137520 mRNA. Translation: CAB70786.2.
AK021559 mRNA. Translation: BAG51038.1.
AB209833 mRNA. Translation: BAD93070.1.
AL031178 Genomic DNA. Translation: CAI20262.1.
AL031178 Genomic DNA. Translation: CAI20263.1.
BC000650 mRNA. Translation: AAH00650.2.
AF174597 mRNA. Translation: AAF04518.1.
CCDSiCCDS55022.1. [Q9UK97-2]
CCDS55023.1. [Q9UK97-1]
CCDS55024.1. [Q9UK97-3]
PIRiT46366.
RefSeqiNP_036479.1. NM_012347.4. [Q9UK97-1]
NP_258441.1. NM_033480.2. [Q9UK97-2]
NP_258442.2. NM_033481.3. [Q9UK97-3]
XP_005249052.1. XM_005248995.4. [Q9UK97-3]
XP_005249053.1. XM_005248996.4. [Q9UK97-3]
UniGeneiHs.216653.

Genome annotation databases

EnsembliENST00000244426; ENSP00000244426; ENSG00000112146. [Q9UK97-1]
ENST00000323557; ENSP00000326968; ENSG00000112146. [Q9UK97-2]
ENST00000370939; ENSP00000359977; ENSG00000112146. [Q9UK97-3]
GeneIDi26268.
KEGGihsa:26268.
UCSCiuc063pbc.1. human. [Q9UK97-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176704 mRNA. Translation: AAF03704.1.
AY858798 mRNA. Translation: AAW51115.1.
AL137520 mRNA. Translation: CAB70786.2.
AK021559 mRNA. Translation: BAG51038.1.
AB209833 mRNA. Translation: BAD93070.1.
AL031178 Genomic DNA. Translation: CAI20262.1.
AL031178 Genomic DNA. Translation: CAI20263.1.
BC000650 mRNA. Translation: AAH00650.2.
AF174597 mRNA. Translation: AAF04518.1.
CCDSiCCDS55022.1. [Q9UK97-2]
CCDS55023.1. [Q9UK97-1]
CCDS55024.1. [Q9UK97-3]
PIRiT46366.
RefSeqiNP_036479.1. NM_012347.4. [Q9UK97-1]
NP_258441.1. NM_033480.2. [Q9UK97-2]
NP_258442.2. NM_033481.3. [Q9UK97-3]
XP_005249052.1. XM_005248995.4. [Q9UK97-3]
XP_005249053.1. XM_005248996.4. [Q9UK97-3]
UniGeneiHs.216653.

3D structure databases

ProteinModelPortaliQ9UK97.
SMRiQ9UK97. Positions 186-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117652. 9 interactions.
DIPiDIP-60458N.
IntActiQ9UK97. 6 interactions.
STRINGi9606.ENSP00000244426.

PTM databases

iPTMnetiQ9UK97.
PhosphoSiteiQ9UK97.

Polymorphism and mutation databases

BioMutaiFBXO9.
DMDMi13124238.

Proteomic databases

EPDiQ9UK97.
MaxQBiQ9UK97.
PaxDbiQ9UK97.
PeptideAtlasiQ9UK97.
PRIDEiQ9UK97.

Protocols and materials databases

DNASUi26268.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000244426; ENSP00000244426; ENSG00000112146. [Q9UK97-1]
ENST00000323557; ENSP00000326968; ENSG00000112146. [Q9UK97-2]
ENST00000370939; ENSP00000359977; ENSG00000112146. [Q9UK97-3]
GeneIDi26268.
KEGGihsa:26268.
UCSCiuc063pbc.1. human. [Q9UK97-1]

Organism-specific databases

CTDi26268.
GeneCardsiFBXO9.
HGNCiHGNC:13588. FBXO9.
HPAiHPA064246.
MIMi609091. gene.
neXtProtiNX_Q9UK97.
PharmGKBiPA28049.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2997. Eukaryota.
ENOG410XPVI. LUCA.
GeneTreeiENSGT00390000014256.
HOGENOMiHOG000047162.
HOVERGENiHBG001041.
InParanoidiQ9UK97.
KOiK10295.
OMAiLVWIHHS.
OrthoDBiEOG091G0BDF.
PhylomeDBiQ9UK97.
TreeFamiTF324797.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiFBXO9. human.
GeneWikiiFBXO9.
GenomeRNAii26268.
PROiQ9UK97.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112146.
CleanExiHS_FBXO9.
ExpressionAtlasiQ9UK97. baseline and differential.
GenevisibleiQ9UK97. HS.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
InterProiIPR001810. F-box_dom.
IPR007330. MIT.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBX9_HUMAN
AccessioniPrimary (citable) accession number: Q9UK97
Secondary accession number(s): A6NFW3
, B3KMM6, O75986, Q59EH8, Q6PKH7, Q9NT57, Q9Y593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Overexpressed in multiple myeloma leading to constitutive activation of the PI3K/mTORC2/Akt pathway to promote survival.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.