Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9UK73

- FEM1B_HUMAN

UniProt

Q9UK73 - FEM1B_HUMAN

Protein

Protein fem-1 homolog B

Gene

FEM1B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei342 – 3432Cleavage; by a caspase-3-like protease

    GO - Molecular functioni

    1. death receptor binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB
    2. branching involved in prostate gland morphogenesis Source: Ensembl
    3. epithelial cell maturation involved in prostate gland development Source: Ensembl
    4. regulation of DNA damage checkpoint Source: UniProtKB
    5. regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
    6. regulation of ubiquitin-protein transferase activity Source: UniProtKB

    Keywords - Biological processi

    Apoptosis, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein fem-1 homolog B
    Short name:
    FEM1b
    Alternative name(s):
    FEM1-beta
    Fem-1-like death receptor-binding protein alpha
    Fem-1-like in apoptotic pathway protein alpha
    Short name:
    F1A-alpha
    Gene namesi
    Name:FEM1B
    Synonyms:F1AA, KIAA0396
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:3649. FEM1B.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi342 – 3421D → A: Prevents cleavage by a caspase-3-like protease. 1 Publication
    Mutagenesisi356 – 3561D → A: Does not affect cleavage by a caspase-3-like protease. 1 Publication

    Organism-specific databases

    PharmGKBiPA28089.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 627627Protein fem-1 homolog BPRO_0000324530Add
    BLAST

    Proteomic databases

    MaxQBiQ9UK73.
    PaxDbiQ9UK73.
    PRIDEiQ9UK73.

    PTM databases

    PhosphoSiteiQ9UK73.

    Miscellaneous databases

    PMAP-CutDBQ9UK73.

    Expressioni

    Tissue specificityi

    Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.1 Publication

    Gene expression databases

    ArrayExpressiQ9UK73.
    BgeeiQ9UK73.
    CleanExiHS_FEM1B.
    GenevestigatoriQ9UK73.

    Organism-specific databases

    HPAiHPA041920.
    HPA042192.

    Interactioni

    Subunit structurei

    Homooligomer. Component of a probable ECS E3 ubiquitin-protein ligase complex containing CUL2, RBX1, TCEB1, TCEB2 and FEM1B. Interacts with PPM1F and PHTF1. Interacts with the death domain of FAS/TNFRSF6 and TNFRSF1A. Interacts with CHEK1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPM1FP495932EBI-310482,EBI-719945

    Protein-protein interaction databases

    BioGridi115421. 25 interactions.
    IntActiQ9UK73. 19 interactions.
    MINTiMINT-128836.
    STRINGi9606.ENSP00000307298.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UK73.
    SMRiQ9UK73. Positions 3-347, 483-590.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati45 – 7430ANK 1Add
    BLAST
    Repeati87 – 11630ANK 2Add
    BLAST
    Repeati120 – 14930ANK 3Add
    BLAST
    Repeati153 – 18230ANK 4Add
    BLAST
    Repeati186 – 21530ANK 5Add
    BLAST
    Repeati218 – 24831ANK 6Add
    BLAST
    Repeati344 – 37734TPRAdd
    BLAST
    Repeati483 – 52745ANK 7Add
    BLAST
    Repeati531 – 56838ANK 8Add
    BLAST

    Sequence similaritiesi

    Belongs to the fem-1 family.Curated
    Contains 8 ANK repeats.PROSITE-ProRule annotation
    Contains 1 TPR repeat.Curated

    Keywords - Domaini

    ANK repeat, Repeat, TPR repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    HOGENOMiHOG000008180.
    HOVERGENiHBG057774.
    InParanoidiQ9UK73.
    KOiK10349.
    OMAiSAMDNYE.
    OrthoDBiEOG7T4MJT.
    PhylomeDBiQ9UK73.
    TreeFamiTF351376.

    Family and domain databases

    Gene3Di1.25.40.20. 4 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view]
    PfamiPF00023. Ank. 7 hits.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 8 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9UK73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGLAGYVYK AASEGKVLTL AALLLNRSES DIRYLLGYVS QQGGQRSTPL    50
    IIAARNGHAK VVRLLLEHYR VQTQQTGTVR FDGYVIDGAT ALWCAAGAGH 100
    FEVVKLLVSH GANVNHTTVT NSTPLRAACF DGRLDIVKYL VENNANISIA 150
    NKYDNTCLMI AAYKGHTDVV RYLLEQRADP NAKAHCGATA LHFAAEAGHI 200
    DIVKELIKWR AAIVVNGHGM TPLKVAAESC KADVVELLLS HADCDRRSRI 250
    EALELLGASF ANDRENYDII KTYHYLYLAM LERFQDGDNI LEKEVLPPIH 300
    AYGNRTECRN PQELESIRQD RDALHMEGLI VRERILGADN IDVSHPIIYR 350
    GAVYADNMEF EQCIKLWLHA LHLRQKGNRN THKDLLRFAQ VFSQMIHLNE 400
    TVKAPDIECV LRCSVLEIEQ SMNRVKNISD ADVHNAMDNY ECNLYTFLYL 450
    VCISTKTQCS EEDQCKINKQ IYNLIHLDPR TREGFTLLHL AVNSNTPVDD 500
    FHTNDVCSFP NALVTKLLLD CGAEVNAVDN EGNSALHIIV QYNRPISDFL 550
    TLHSIIISLV EAGAHTDMTN KQNKTPLDKS TTGVSEILLK TQMKMSLKCL 600
    AARAVRANDI NYQDQIPRTL EEFVGFH 627
    Length:627
    Mass (Da):70,264
    Last modified:May 1, 2000 - v1
    Checksum:i85DA943663A988C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF178632 mRNA. Translation: AAF05314.1.
    AF204883 mRNA. Translation: AAF69303.1.
    AB007856 mRNA. Translation: BAA23692.2.
    AK290167 mRNA. Translation: BAF82856.1.
    AC021553 Genomic DNA. No translation available.
    AC107871 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77818.1.
    BC010122 mRNA. Translation: AAH10122.1.
    BC014558 mRNA. Translation: AAH14558.1.
    CCDSiCCDS10228.1.
    RefSeqiNP_056137.1. NM_015322.4.
    UniGeneiHs.362733.
    Hs.592737.

    Genome annotation databases

    EnsembliENST00000306917; ENSP00000307298; ENSG00000169018.
    GeneIDi10116.
    KEGGihsa:10116.
    UCSCiuc002arg.3. human.

    Polymorphism databases

    DMDMi74753369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF178632 mRNA. Translation: AAF05314.1 .
    AF204883 mRNA. Translation: AAF69303.1 .
    AB007856 mRNA. Translation: BAA23692.2 .
    AK290167 mRNA. Translation: BAF82856.1 .
    AC021553 Genomic DNA. No translation available.
    AC107871 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77818.1 .
    BC010122 mRNA. Translation: AAH10122.1 .
    BC014558 mRNA. Translation: AAH14558.1 .
    CCDSi CCDS10228.1.
    RefSeqi NP_056137.1. NM_015322.4.
    UniGenei Hs.362733.
    Hs.592737.

    3D structure databases

    ProteinModelPortali Q9UK73.
    SMRi Q9UK73. Positions 3-347, 483-590.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115421. 25 interactions.
    IntActi Q9UK73. 19 interactions.
    MINTi MINT-128836.
    STRINGi 9606.ENSP00000307298.

    PTM databases

    PhosphoSitei Q9UK73.

    Polymorphism databases

    DMDMi 74753369.

    Proteomic databases

    MaxQBi Q9UK73.
    PaxDbi Q9UK73.
    PRIDEi Q9UK73.

    Protocols and materials databases

    DNASUi 10116.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000306917 ; ENSP00000307298 ; ENSG00000169018 .
    GeneIDi 10116.
    KEGGi hsa:10116.
    UCSCi uc002arg.3. human.

    Organism-specific databases

    CTDi 10116.
    GeneCardsi GC15P068570.
    HGNCi HGNC:3649. FEM1B.
    HPAi HPA041920.
    HPA042192.
    MIMi 613539. gene.
    neXtProti NX_Q9UK73.
    PharmGKBi PA28089.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0666.
    HOGENOMi HOG000008180.
    HOVERGENi HBG057774.
    InParanoidi Q9UK73.
    KOi K10349.
    OMAi SAMDNYE.
    OrthoDBi EOG7T4MJT.
    PhylomeDBi Q9UK73.
    TreeFami TF351376.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    GenomeRNAii 10116.
    NextBioi 38269.
    PMAP-CutDB Q9UK73.
    PROi Q9UK73.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UK73.
    Bgeei Q9UK73.
    CleanExi HS_FEM1B.
    Genevestigatori Q9UK73.

    Family and domain databases

    Gene3Di 1.25.40.20. 4 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    [Graphical view ]
    Pfami PF00023. Ank. 7 hits.
    [Graphical view ]
    PRINTSi PR01415. ANKYRIN.
    SMARTi SM00248. ANK. 8 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 2 hits.
    PROSITEi PS50297. ANK_REP_REGION. 2 hits.
    PS50088. ANK_REPEAT. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis."
      Chan S.-L., Tan K.-O., Zhang L., Yee K.S.Y., Ronca F., Chan M.-Y., Yu V.C.
      J. Biol. Chem. 274:32461-32468(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CLEAVAGE, TISSUE SPECIFICITY, INTERACTION WITH FAS AND TNFRSF1A, MUTAGENESIS OF ASP-342 AND ASP-356.
    2. "Sequence, organization, and expression of the human FEM1B gene."
      Ventura-Holman T., Maher J.F.
      Biochem. Biophys. Res. Commun. 267:317-320(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
      Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thalamus.
    6. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    9. "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."
      Tan K.M.L., Chan S.-L., Tan K.O., Yu V.C.
      J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPM1F.
    10. "VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases."
      Kamura T., Maenaka K., Kotoshiba S., Matsumoto M., Kohda D., Conaway R.C., Conaway J.W., Nakayama K.I.
      Genes Dev. 18:3055-3065(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN E3 UBIQUITIN-PROTEIN LIGASE COMPLEX WITH CUL2.
    11. "Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress."
      Sun T.P., Shieh S.Y.
      Oncogene 28:1971-1981(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CHEK1.

    Entry informationi

    Entry nameiFEM1B_HUMAN
    AccessioniPrimary (citable) accession number: Q9UK73
    Secondary accession number(s): O43146
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3