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Q9UK73

- FEM1B_HUMAN

UniProt

Q9UK73 - FEM1B_HUMAN

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Protein

Protein fem-1 homolog B

Gene

FEM1B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei342 – 3432Cleavage; by a caspase-3-like protease

GO - Molecular functioni

  1. death receptor binding Source: UniProtKB
  2. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. branching involved in prostate gland morphogenesis Source: Ensembl
  3. epithelial cell maturation involved in prostate gland development Source: Ensembl
  4. regulation of DNA damage checkpoint Source: UniProtKB
  5. regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  6. regulation of ubiquitin-protein transferase activity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein fem-1 homolog B
Short name:
FEM1b
Alternative name(s):
FEM1-beta
Fem-1-like death receptor-binding protein alpha
Fem-1-like in apoptotic pathway protein alpha
Short name:
F1A-alpha
Gene namesi
Name:FEM1B
Synonyms:F1AA, KIAA0396
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:3649. FEM1B.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi342 – 3421D → A: Prevents cleavage by a caspase-3-like protease. 1 Publication
Mutagenesisi356 – 3561D → A: Does not affect cleavage by a caspase-3-like protease. 1 Publication

Organism-specific databases

PharmGKBiPA28089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 627627Protein fem-1 homolog BPRO_0000324530Add
BLAST

Proteomic databases

MaxQBiQ9UK73.
PaxDbiQ9UK73.
PRIDEiQ9UK73.

PTM databases

PhosphoSiteiQ9UK73.

Miscellaneous databases

PMAP-CutDBQ9UK73.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.1 Publication

Gene expression databases

BgeeiQ9UK73.
CleanExiHS_FEM1B.
ExpressionAtlasiQ9UK73. baseline and differential.
GenevestigatoriQ9UK73.

Organism-specific databases

HPAiHPA041920.
HPA042192.

Interactioni

Subunit structurei

Homooligomer. Component of a probable ECS E3 ubiquitin-protein ligase complex containing CUL2, RBX1, TCEB1, TCEB2 and FEM1B. Interacts with PPM1F and PHTF1. Interacts with the death domain of FAS/TNFRSF6 and TNFRSF1A. Interacts with CHEK1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPM1FP495932EBI-310482,EBI-719945

Protein-protein interaction databases

BioGridi115421. 34 interactions.
IntActiQ9UK73. 19 interactions.
MINTiMINT-128836.
STRINGi9606.ENSP00000307298.

Structurei

3D structure databases

ProteinModelPortaliQ9UK73.
SMRiQ9UK73. Positions 5-300, 477-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 7430ANK 1Add
BLAST
Repeati87 – 11630ANK 2Add
BLAST
Repeati120 – 14930ANK 3Add
BLAST
Repeati153 – 18230ANK 4Add
BLAST
Repeati186 – 21530ANK 5Add
BLAST
Repeati218 – 24831ANK 6Add
BLAST
Repeati344 – 37734TPRAdd
BLAST
Repeati483 – 52745ANK 7Add
BLAST
Repeati531 – 56838ANK 8Add
BLAST

Sequence similaritiesi

Belongs to the fem-1 family.Curated
Contains 8 ANK repeats.PROSITE-ProRule annotation
Contains 1 TPR repeat.Curated

Keywords - Domaini

ANK repeat, Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000118867.
HOGENOMiHOG000008180.
HOVERGENiHBG057774.
InParanoidiQ9UK73.
KOiK10349.
OMAiSAMDNYE.
OrthoDBiEOG7T4MJT.
PhylomeDBiQ9UK73.
TreeFamiTF351376.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 7 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UK73-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGLAGYVYK AASEGKVLTL AALLLNRSES DIRYLLGYVS QQGGQRSTPL
60 70 80 90 100
IIAARNGHAK VVRLLLEHYR VQTQQTGTVR FDGYVIDGAT ALWCAAGAGH
110 120 130 140 150
FEVVKLLVSH GANVNHTTVT NSTPLRAACF DGRLDIVKYL VENNANISIA
160 170 180 190 200
NKYDNTCLMI AAYKGHTDVV RYLLEQRADP NAKAHCGATA LHFAAEAGHI
210 220 230 240 250
DIVKELIKWR AAIVVNGHGM TPLKVAAESC KADVVELLLS HADCDRRSRI
260 270 280 290 300
EALELLGASF ANDRENYDII KTYHYLYLAM LERFQDGDNI LEKEVLPPIH
310 320 330 340 350
AYGNRTECRN PQELESIRQD RDALHMEGLI VRERILGADN IDVSHPIIYR
360 370 380 390 400
GAVYADNMEF EQCIKLWLHA LHLRQKGNRN THKDLLRFAQ VFSQMIHLNE
410 420 430 440 450
TVKAPDIECV LRCSVLEIEQ SMNRVKNISD ADVHNAMDNY ECNLYTFLYL
460 470 480 490 500
VCISTKTQCS EEDQCKINKQ IYNLIHLDPR TREGFTLLHL AVNSNTPVDD
510 520 530 540 550
FHTNDVCSFP NALVTKLLLD CGAEVNAVDN EGNSALHIIV QYNRPISDFL
560 570 580 590 600
TLHSIIISLV EAGAHTDMTN KQNKTPLDKS TTGVSEILLK TQMKMSLKCL
610 620
AARAVRANDI NYQDQIPRTL EEFVGFH
Length:627
Mass (Da):70,264
Last modified:May 1, 2000 - v1
Checksum:i85DA943663A988C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF178632 mRNA. Translation: AAF05314.1.
AF204883 mRNA. Translation: AAF69303.1.
AB007856 mRNA. Translation: BAA23692.2.
AK290167 mRNA. Translation: BAF82856.1.
AC021553 Genomic DNA. No translation available.
AC107871 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77818.1.
BC010122 mRNA. Translation: AAH10122.1.
BC014558 mRNA. Translation: AAH14558.1.
CCDSiCCDS10228.1.
RefSeqiNP_056137.1. NM_015322.4.
UniGeneiHs.362733.
Hs.592737.

Genome annotation databases

EnsembliENST00000306917; ENSP00000307298; ENSG00000169018.
GeneIDi10116.
KEGGihsa:10116.
UCSCiuc002arg.3. human.

Polymorphism databases

DMDMi74753369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF178632 mRNA. Translation: AAF05314.1 .
AF204883 mRNA. Translation: AAF69303.1 .
AB007856 mRNA. Translation: BAA23692.2 .
AK290167 mRNA. Translation: BAF82856.1 .
AC021553 Genomic DNA. No translation available.
AC107871 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77818.1 .
BC010122 mRNA. Translation: AAH10122.1 .
BC014558 mRNA. Translation: AAH14558.1 .
CCDSi CCDS10228.1.
RefSeqi NP_056137.1. NM_015322.4.
UniGenei Hs.362733.
Hs.592737.

3D structure databases

ProteinModelPortali Q9UK73.
SMRi Q9UK73. Positions 5-300, 477-596.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115421. 34 interactions.
IntActi Q9UK73. 19 interactions.
MINTi MINT-128836.
STRINGi 9606.ENSP00000307298.

PTM databases

PhosphoSitei Q9UK73.

Polymorphism databases

DMDMi 74753369.

Proteomic databases

MaxQBi Q9UK73.
PaxDbi Q9UK73.
PRIDEi Q9UK73.

Protocols and materials databases

DNASUi 10116.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000306917 ; ENSP00000307298 ; ENSG00000169018 .
GeneIDi 10116.
KEGGi hsa:10116.
UCSCi uc002arg.3. human.

Organism-specific databases

CTDi 10116.
GeneCardsi GC15P068570.
HGNCi HGNC:3649. FEM1B.
HPAi HPA041920.
HPA042192.
MIMi 613539. gene.
neXtProti NX_Q9UK73.
PharmGKBi PA28089.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00760000118867.
HOGENOMi HOG000008180.
HOVERGENi HBG057774.
InParanoidi Q9UK73.
KOi K10349.
OMAi SAMDNYE.
OrthoDBi EOG7T4MJT.
PhylomeDBi Q9UK73.
TreeFami TF351376.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

GenomeRNAii 10116.
NextBioi 38269.
PMAP-CutDB Q9UK73.
PROi Q9UK73.
SOURCEi Search...

Gene expression databases

Bgeei Q9UK73.
CleanExi HS_FEM1B.
ExpressionAtlasi Q9UK73. baseline and differential.
Genevestigatori Q9UK73.

Family and domain databases

Gene3Di 1.25.40.20. 4 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view ]
Pfami PF00023. Ank. 7 hits.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 8 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 2 hits.
PROSITEi PS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis."
    Chan S.-L., Tan K.-O., Zhang L., Yee K.S.Y., Ronca F., Chan M.-Y., Yu V.C.
    J. Biol. Chem. 274:32461-32468(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CLEAVAGE, TISSUE SPECIFICITY, INTERACTION WITH FAS AND TNFRSF1A, MUTAGENESIS OF ASP-342 AND ASP-356.
  2. "Sequence, organization, and expression of the human FEM1B gene."
    Ventura-Holman T., Maher J.F.
    Biochem. Biophys. Res. Commun. 267:317-320(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."
    Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thalamus.
  6. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  9. "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."
    Tan K.M.L., Chan S.-L., Tan K.O., Yu V.C.
    J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPM1F.
  10. "VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases."
    Kamura T., Maenaka K., Kotoshiba S., Matsumoto M., Kohda D., Conaway R.C., Conaway J.W., Nakayama K.I.
    Genes Dev. 18:3055-3065(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN E3 UBIQUITIN-PROTEIN LIGASE COMPLEX WITH CUL2.
  11. "Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress."
    Sun T.P., Shieh S.Y.
    Oncogene 28:1971-1981(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CHEK1.

Entry informationi

Entry nameiFEM1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UK73
Secondary accession number(s): O43146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3