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Protein

Protein fem-1 homolog B

Gene

FEM1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • death receptor binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • branching involved in prostate gland morphogenesis Source: Ensembl
  • epithelial cell maturation involved in prostate gland development Source: Ensembl
  • regulation of DNA damage checkpoint Source: UniProtKB
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
  • regulation of ubiquitin-protein transferase activity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169018-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein fem-1 homolog B
Short name:
FEM1b
Alternative name(s):
FEM1-beta
Fem-1-like death receptor-binding protein alpha
Fem-1-like in apoptotic pathway protein alpha
Short name:
F1A-alpha
Gene namesi
Name:FEM1B
Synonyms:F1AA, KIAA0396
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:3649. FEM1B.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi342D → A: Prevents cleavage by a caspase-3-like protease. 1 Publication1
Mutagenesisi356D → A: Does not affect cleavage by a caspase-3-like protease. 1 Publication1

Organism-specific databases

DisGeNETi10116.
OpenTargetsiENSG00000169018.
PharmGKBiPA28089.

Polymorphism and mutation databases

DMDMi74753369.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245301 – 627Protein fem-1 homolog BAdd BLAST627

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei342 – 343Cleavage; by a caspase-3-like protease2

Proteomic databases

EPDiQ9UK73.
MaxQBiQ9UK73.
PaxDbiQ9UK73.
PeptideAtlasiQ9UK73.
PRIDEiQ9UK73.

PTM databases

iPTMnetiQ9UK73.
PhosphoSitePlusiQ9UK73.

Miscellaneous databases

PMAP-CutDBQ9UK73.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSG00000169018.
CleanExiHS_FEM1B.
ExpressionAtlasiQ9UK73. baseline and differential.
GenevisibleiQ9UK73. HS.

Organism-specific databases

HPAiHPA041920.
HPA042192.

Interactioni

Subunit structurei

Homooligomer. Component of a probable ECS E3 ubiquitin-protein ligase complex containing CUL2, RBX1, TCEB1, TCEB2 and FEM1B. Interacts with PPM1F and PHTF1. Interacts with the death domain of FAS/TNFRSF6 and TNFRSF1A. Interacts with CHEK1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPM1FP495932EBI-310482,EBI-719945

GO - Molecular functioni

  • death receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115421. 51 interactors.
DIPiDIP-31663N.
IntActiQ9UK73. 21 interactors.
MINTiMINT-128836.
STRINGi9606.ENSP00000307298.

Structurei

3D structure databases

ProteinModelPortaliQ9UK73.
SMRiQ9UK73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 74ANK 1Add BLAST30
Repeati87 – 116ANK 2Add BLAST30
Repeati120 – 149ANK 3Add BLAST30
Repeati153 – 182ANK 4Add BLAST30
Repeati186 – 215ANK 5Add BLAST30
Repeati218 – 248ANK 6Add BLAST31
Repeati344 – 377TPRAdd BLAST34
Repeati483 – 527ANK 7Add BLAST45
Repeati531 – 568ANK 8Add BLAST38

Sequence similaritiesi

Belongs to the fem-1 family.Curated
Contains 8 ANK repeats.PROSITE-ProRule annotation
Contains 1 TPR repeat.Curated

Keywords - Domaini

ANK repeat, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0508. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129724.
HOGENOMiHOG000008180.
HOVERGENiHBG057774.
InParanoidiQ9UK73.
KOiK10349.
OMAiERYRDSE.
OrthoDBiEOG091G03I9.
PhylomeDBiQ9UK73.
TreeFamiTF351376.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UK73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGLAGYVYK AASEGKVLTL AALLLNRSES DIRYLLGYVS QQGGQRSTPL
60 70 80 90 100
IIAARNGHAK VVRLLLEHYR VQTQQTGTVR FDGYVIDGAT ALWCAAGAGH
110 120 130 140 150
FEVVKLLVSH GANVNHTTVT NSTPLRAACF DGRLDIVKYL VENNANISIA
160 170 180 190 200
NKYDNTCLMI AAYKGHTDVV RYLLEQRADP NAKAHCGATA LHFAAEAGHI
210 220 230 240 250
DIVKELIKWR AAIVVNGHGM TPLKVAAESC KADVVELLLS HADCDRRSRI
260 270 280 290 300
EALELLGASF ANDRENYDII KTYHYLYLAM LERFQDGDNI LEKEVLPPIH
310 320 330 340 350
AYGNRTECRN PQELESIRQD RDALHMEGLI VRERILGADN IDVSHPIIYR
360 370 380 390 400
GAVYADNMEF EQCIKLWLHA LHLRQKGNRN THKDLLRFAQ VFSQMIHLNE
410 420 430 440 450
TVKAPDIECV LRCSVLEIEQ SMNRVKNISD ADVHNAMDNY ECNLYTFLYL
460 470 480 490 500
VCISTKTQCS EEDQCKINKQ IYNLIHLDPR TREGFTLLHL AVNSNTPVDD
510 520 530 540 550
FHTNDVCSFP NALVTKLLLD CGAEVNAVDN EGNSALHIIV QYNRPISDFL
560 570 580 590 600
TLHSIIISLV EAGAHTDMTN KQNKTPLDKS TTGVSEILLK TQMKMSLKCL
610 620
AARAVRANDI NYQDQIPRTL EEFVGFH
Length:627
Mass (Da):70,264
Last modified:May 1, 2000 - v1
Checksum:i85DA943663A988C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178632 mRNA. Translation: AAF05314.1.
AF204883 mRNA. Translation: AAF69303.1.
AB007856 mRNA. Translation: BAA23692.2.
AK290167 mRNA. Translation: BAF82856.1.
AC021553 Genomic DNA. No translation available.
AC107871 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77818.1.
BC010122 mRNA. Translation: AAH10122.1.
BC014558 mRNA. Translation: AAH14558.1.
CCDSiCCDS10228.1.
RefSeqiNP_056137.1. NM_015322.4.
UniGeneiHs.362733.
Hs.592737.

Genome annotation databases

EnsembliENST00000306917; ENSP00000307298; ENSG00000169018.
GeneIDi10116.
KEGGihsa:10116.
UCSCiuc002arg.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178632 mRNA. Translation: AAF05314.1.
AF204883 mRNA. Translation: AAF69303.1.
AB007856 mRNA. Translation: BAA23692.2.
AK290167 mRNA. Translation: BAF82856.1.
AC021553 Genomic DNA. No translation available.
AC107871 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77818.1.
BC010122 mRNA. Translation: AAH10122.1.
BC014558 mRNA. Translation: AAH14558.1.
CCDSiCCDS10228.1.
RefSeqiNP_056137.1. NM_015322.4.
UniGeneiHs.362733.
Hs.592737.

3D structure databases

ProteinModelPortaliQ9UK73.
SMRiQ9UK73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115421. 51 interactors.
DIPiDIP-31663N.
IntActiQ9UK73. 21 interactors.
MINTiMINT-128836.
STRINGi9606.ENSP00000307298.

PTM databases

iPTMnetiQ9UK73.
PhosphoSitePlusiQ9UK73.

Polymorphism and mutation databases

DMDMi74753369.

Proteomic databases

EPDiQ9UK73.
MaxQBiQ9UK73.
PaxDbiQ9UK73.
PeptideAtlasiQ9UK73.
PRIDEiQ9UK73.

Protocols and materials databases

DNASUi10116.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306917; ENSP00000307298; ENSG00000169018.
GeneIDi10116.
KEGGihsa:10116.
UCSCiuc002arg.4. human.

Organism-specific databases

CTDi10116.
DisGeNETi10116.
GeneCardsiFEM1B.
HGNCiHGNC:3649. FEM1B.
HPAiHPA041920.
HPA042192.
MIMi613539. gene.
neXtProtiNX_Q9UK73.
OpenTargetsiENSG00000169018.
PharmGKBiPA28089.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0508. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129724.
HOGENOMiHOG000008180.
HOVERGENiHBG057774.
InParanoidiQ9UK73.
KOiK10349.
OMAiERYRDSE.
OrthoDBiEOG091G03I9.
PhylomeDBiQ9UK73.
TreeFamiTF351376.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000169018-MONOMER.

Miscellaneous databases

ChiTaRSiFEM1B. human.
GenomeRNAii10116.
PMAP-CutDBQ9UK73.
PROiQ9UK73.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169018.
CleanExiHS_FEM1B.
ExpressionAtlasiQ9UK73. baseline and differential.
GenevisibleiQ9UK73. HS.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEM1B_HUMAN
AccessioniPrimary (citable) accession number: Q9UK73
Secondary accession number(s): O43146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.