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Q9UK59

- DBR1_HUMAN

UniProt

Q9UK59 - DBR1_HUMAN

Protein

Lariat debranching enzyme

Gene

DBR1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (19 Sep 2006)
      Previous versions | rss
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    Functioni

    Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis.2 Publications

    Catalytic activityi

    RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats.

    Cofactori

    Divalent metal cations.1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. poly(A) RNA binding Source: UniProtKB
    3. RNA lariat debranching enzyme activity Source: UniProtKB

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: Ensembl
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. RNA splicing, via transesterification reactions Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lariat debranching enzyme (EC:3.1.-.-)
    Gene namesi
    Name:DBR1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:15594. DBR1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27166.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 544544Lariat debranching enzymePRO_0000250358Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281N6-acetyllysine1 Publication
    Modified residuei474 – 4741Phosphoserine1 Publication
    Modified residuei478 – 4781Phosphoserine1 Publication
    Modified residuei479 – 4791Phosphoserine1 Publication
    Modified residuei499 – 4991PhosphoserineBy similarity
    Modified residuei514 – 5141Phosphoserine3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9UK59.
    PaxDbiQ9UK59.
    PeptideAtlasiQ9UK59.
    PRIDEiQ9UK59.

    PTM databases

    PhosphoSiteiQ9UK59.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UK59.
    BgeeiQ9UK59.
    CleanExiHS_DBR1.
    GenevestigatoriQ9UK59.

    Organism-specific databases

    HPAiHPA035365.

    Interactioni

    Protein-protein interaction databases

    BioGridi119344. 4 interactions.
    IntActiQ9UK59. 3 interactions.
    STRINGi9606.ENSP00000260803.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UK59.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the lariat debranching enzyme family.Curated

    Phylogenomic databases

    eggNOGiNOG133819.
    HOGENOMiHOG000216468.
    HOVERGENiHBG079917.
    InParanoidiQ9UK59.
    KOiK18328.
    OMAiKVPCNFS.
    OrthoDBiEOG7BW0J7.
    PhylomeDBiQ9UK59.
    TreeFamiTF313221.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR007708. DBR1_C.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF05011. DBR1. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UK59-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRVAVAGCCH GELDKIYETL ALAERRGPGP VDLLLCCGDF QAVRNEADLR    50
    CMAVPPKYRH MQTFYRYYSG EKKAPVLTLF IGGNHEASNH LQELPYGGWV 100
    APNIYYLGLA GVVKYRGVRI GGISGIFKSH DYRKGHFECP PYNSSTIRSI 150
    YHVRNIEVYK LKQLKQPIDI FLSHDWPRSI YHYGNKKQLL KTKSFFRQEV 200
    ENNTLGSPAA SELLEHLKPT YWFSAHLHVK FAALMQHQAK DKGQTARATK 250
    FLALDKCLPH RDFLQILEIE HDPSAPDYLE YDIEWLTILR ATDDLINVTG 300
    RLWNMPENNG LHARWDYSAT EEGMKEVLEK LNHDLKVPCN FSVTAACYDP 350
    SKPQTQMQLI HRINPQTTEF CAQLGIIDIN VRLQKSKEEH HVCGEYEEQD 400
    DVESNDSGED QSEYNTDTSA LSSINPDEIM LDEEEDEDSI VSAHSGMNTP 450
    SVEPSDQASE FSASFSDVRI LPGSMIVSSD DTVDSTIDRE GKPGGTVESG 500
    NGEDLTKVPL KRLSDEHEPE QRKKIKRRNQ AIYAAVDDDD DDAA 544
    Length:544
    Mass (Da):61,555
    Last modified:September 19, 2006 - v2
    Checksum:iDDA5F4FADA194BAA
    GO
    Isoform 2 (identifier: Q9UK59-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-232: Missing.
         233-237: ALMQH → MIHIV

    Note: No experimental confirmation available.

    Show »
    Length:312
    Mass (Da):35,022
    Checksum:i4E874B871647E9F5
    GO

    Sequence cautioni

    The sequence AAD53327.2 differs from that shown. Reason: Frameshift at positions 450 and 463.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti308 – 3081N → S in BAA90954. (PubMed:14702039)Curated
    Sequence conflicti496 – 4961T → L in AAD53327. (PubMed:10982890)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 232232Missing in isoform 2. 1 PublicationVSP_020631Add
    BLAST
    Alternative sequencei233 – 2375ALMQH → MIHIV in isoform 2. 1 PublicationVSP_020632

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF180919 mRNA. Translation: AAD53327.2. Frameshift.
    AK000116 mRNA. Translation: BAA90954.1.
    BC009472 mRNA. Translation: AAH09472.1.
    CCDSiCCDS33863.1. [Q9UK59-1]
    RefSeqiNP_057300.2. NM_016216.3. [Q9UK59-1]
    UniGeneiHs.477700.

    Genome annotation databases

    EnsembliENST00000260803; ENSP00000260803; ENSG00000138231. [Q9UK59-1]
    GeneIDi51163.
    KEGGihsa:51163.
    UCSCiuc003ert.3. human. [Q9UK59-2]
    uc003eru.3. human. [Q9UK59-1]

    Polymorphism databases

    DMDMi115311701.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF180919 mRNA. Translation: AAD53327.2 . Frameshift.
    AK000116 mRNA. Translation: BAA90954.1 .
    BC009472 mRNA. Translation: AAH09472.1 .
    CCDSi CCDS33863.1. [Q9UK59-1 ]
    RefSeqi NP_057300.2. NM_016216.3. [Q9UK59-1 ]
    UniGenei Hs.477700.

    3D structure databases

    ProteinModelPortali Q9UK59.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119344. 4 interactions.
    IntActi Q9UK59. 3 interactions.
    STRINGi 9606.ENSP00000260803.

    PTM databases

    PhosphoSitei Q9UK59.

    Polymorphism databases

    DMDMi 115311701.

    Proteomic databases

    MaxQBi Q9UK59.
    PaxDbi Q9UK59.
    PeptideAtlasi Q9UK59.
    PRIDEi Q9UK59.

    Protocols and materials databases

    DNASUi 51163.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260803 ; ENSP00000260803 ; ENSG00000138231 . [Q9UK59-1 ]
    GeneIDi 51163.
    KEGGi hsa:51163.
    UCSCi uc003ert.3. human. [Q9UK59-2 ]
    uc003eru.3. human. [Q9UK59-1 ]

    Organism-specific databases

    CTDi 51163.
    GeneCardsi GC03M137879.
    HGNCi HGNC:15594. DBR1.
    HPAi HPA035365.
    MIMi 607024. gene.
    neXtProti NX_Q9UK59.
    PharmGKBi PA27166.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG133819.
    HOGENOMi HOG000216468.
    HOVERGENi HBG079917.
    InParanoidi Q9UK59.
    KOi K18328.
    OMAi KVPCNFS.
    OrthoDBi EOG7BW0J7.
    PhylomeDBi Q9UK59.
    TreeFami TF313221.

    Miscellaneous databases

    GeneWikii DBR1.
    GenomeRNAii 51163.
    NextBioi 54089.
    PROi Q9UK59.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UK59.
    Bgeei Q9UK59.
    CleanExi HS_DBR1.
    Genevestigatori Q9UK59.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR007708. DBR1_C.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF05011. DBR1. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants."
      Kim J.-W., Kim H.-C., Kim G.-M., Yang J.-M., Boeke J.D., Nam K.
      Nucleic Acids Res. 28:3666-3673(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Colon.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Purification of a RNA debranching activity from HeLa cells."
      Arenas J., Hurwitz J.
      J. Biol. Chem. 262:4274-4279(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    5. Cited for: FUNCTION.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-478 AND SER-479, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-128, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDBR1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UK59
    Secondary accession number(s): Q96GH0, Q9NXQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Cells lacking DBR1 show an inhibition of HIV-1 replication.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3