Q9UK55 (ZPI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Z-dependent protease inhibitor
Short name=PZ-dependent protease inhibitor
Short name=PZI

Alternative name(s):
Serpin A10

Gene names

Name:	SERPINA10
Synonyms:	ZPI
ORF Names:	UNQ707/PRO1358

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits factor Xa activity in the presence of protein Z, calcium and phospholipid.
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma. Ref.10
Post-translational modification	Phosphorylation sites are present in the extracelllular medium.
Sequence similarities	Belongs to the serpin family.
Sequence caution	The sequence CAD62339.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.6

Chain

22 – 444

423

Protein Z-dependent protease inhibitor

PRO_0000032482

Sites

Site

408 – 409

2

Reactive bond By similarity

Amino acid modifications

Modified residue

56

1

Phosphoserine Ref.10

Modified residue

61

1

Phosphoserine Ref.10

Glycosylation

36

1

N-linked (GlcNAc...) Ref.9

Glycosylation

180

1

N-linked (GlcNAc...) Ref.8

Glycosylation

197

1

N-linked (GlcNAc...) Potential

Glycosylation

295

1

N-linked (GlcNAc...) Ref.8

Natural variations

Natural variant

46

1

K → R. Ref.2 Ref.4
Corresponds to variant rs941590 [ dbSNP | Ensembl ].

VAR_020325

Natural variant

61

1

S → G. Ref.2 Ref.4
Corresponds to variant rs941591 [ dbSNP | Ensembl ].

VAR_020326

Natural variant

139

1

G → R. Ref.4
Corresponds to variant rs56137907 [ dbSNP | Ensembl ].

VAR_038833

Natural variant

158

1

L → Q.
Corresponds to variant rs2232699 [ dbSNP | Ensembl ].

VAR_051940

Natural variant

161

1

T → S. Ref.2 Ref.4
Corresponds to variant rs2232700 [ dbSNP | Ensembl ].

VAR_020327

Natural variant

196

1

R → H.
Corresponds to variant rs2232701 [ dbSNP | Ensembl ].

VAR_051941

Natural variant

271

1

G → S. Ref.4
Corresponds to variant rs2232708 [ dbSNP | Ensembl ].

VAR_038834

Natural variant

384

1

Q → P. Ref.4

VAR_038835

Natural variant

384

1

Q → R. Ref.2
Corresponds to variant rs2232710 [ dbSNP | Ensembl ].

VAR_051942

Experimental info

Mutagenesis

408

1

Y → A: Loss of inhibitory activity. Ref.1

Secondary structure

1

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444

Helix Strand Turn

Details...

Helix

62 – 81

20

Helix

85 – 93

9

Helix

106 – 109

4

Helix

120 – 125

6

Helix

141 – 145

5

Helix

149 – 154

6

Beta strand

165 – 169

5

Helix

176 – 186

11

Beta strand

189 – 193

5

Helix

198 – 212

15

Beta strand

230 – 238

9

Beta strand

242 – 244

3

Helix

248 – 250

3

Beta strand

252 – 259

8

Beta strand

262 – 269

8

Beta strand

275 – 280

6

Turn

281 – 284

4

Beta strand

285 – 292

8

Beta strand

296 – 303

8

Helix

309 – 312

4

Helix

313 – 315

3

Helix

318 – 326

9

Beta strand

329 – 331

3

Beta strand

334 – 338

5

Beta strand

340 – 347

8

Helix

349 – 354

6

Helix

359 – 361

3

Turn

368 – 370

3

Beta strand

381 – 390

10

Beta strand

413 – 415

3

Beta strand

420 – 427

8

Beta strand

432 – 435

4

Sequences

	Sequence		Length	Mass (Da)
	Q9UK55 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 06BD47C97BBDD700	FASTA	444	50,707
	10 20 30 40 50 60 MKVVPSLLLS VLLAQVWLVP GLAPSPQSPE TPAPQNQTSR VVQAPKEEEE DEQEASEEKA 70 80 90 100 110 120 SEEEKAWLMA SRQQLAKETS NFGFSLLRKI SMRHDGNMVF SPFGMSLAMT GLMLGATGPT 130 140 150 160 170 180 ETQIKRGLHL QALKPTKPGL LPSLFKGLRE TLSRNLELGL TQGSFAFIHK DFDVKETFFN 190 200 210 220 230 240 LSKRYFDTEC VPMNFRNASQ AKRLMNHYIN KETRGKIPKL FDEINPETKL ILVDYILFKG 250 260 270 280 290 300 KWLTPFDPVF TEVDTFHLDK YKTIKVPMMY GAGKFASTFD KNFRCHVLKL PYQGNATMLV 310 320 330 340 350 360 VLMEKMGDHL ALEDYLTTDL VETWLRNMKT RNMEVFFPKF KLDQKYEMHE LLRQMGIRRI 370 380 390 400 410 420 FSPFADLSEL SATGRNLQVS RVLQRTVIEV DERGTEAVAG ILSEITAYSM PPVIKVDRPF 430 440 HFMIYEETSG MLLFLGRVVN PTLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The protein Z-dependent protease inhibitor is a serpin." Han X., Huang Z.-F., Fiehler R., Broze G.J. Jr. Biochemistry 38:11073-11078(1999) [PubMed: 10460162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF TYR-408. Tissue: Liver.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-46; GLY-61; SER-161 AND ARG-384.
[3]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal liver.
[4]	SeattleSNPs variation discovery resource Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-46; GLY-61; ARG-139; SER-161; SER-271 AND PRO-384.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[6]	"Isolation of a protein Z-dependent plasma protease inhibitor." Han X., Fiehler R., Broze G.J. Jr. Proc. Natl. Acad. Sci. U.S.A. 95:9250-9255(1998) [PubMed: 9689066] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-33, CHARACTERIZATION. Tissue: Plasma.
[7]	"Characterization of the protein Z-dependent protease inhibitor." Han X., Fiehler R., Broze G.J. Jr. Blood 96:3049-3055(2000) [PubMed: 11049983] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-180 AND ASN-295, MASS SPECTROMETRY. Tissue: Plasma.
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-36, MASS SPECTROMETRY. Tissue: Liver.
[10]	"An initial characterization of the serum phosphoproteome." Zhou W., Ross M.M., Tessitore A., Ornstein D., Vanmeter A., Liotta L.A., Petricoin E.F. III J. Proteome Res. 8:5523-5531(2009) [PubMed: 19824718] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56 AND SER-61, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Serum.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF181467 mRNA. Translation: AAD53962.1.
AY358597 mRNA. Translation: AAQ88960.1.
BX248011 mRNA. Translation: CAD62339.1. Different initiation.
EF621762 Genomic DNA. Translation: ABR09269.1.
BC022261 mRNA. Translation: AAH22261.1.

IPI

IPI00007199.

RefSeq

NP_001094077.1. NM_001100607.1.
NP_057270.1. NM_016186.2.

UniGene

Hs.118620.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F1S	X-ray	2.30	A	60-444	[»]
3H5C	X-ray	3.26	A	22-444	[»]

ProteinModelPortal

Q9UK55.

SMR

Q9UK55. Positions 60-444.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UK55. 1 interaction.

STRING

Q9UK55.

Protein family/group databases

MEROPS

I04.005.

PTM databases

PhosphoSite

Q9UK55.

Polymorphism databases

DMDM

12585541.

Proteomic databases

PRIDE

Q9UK55.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000261994; ENSP00000261994; ENSG00000140093.
ENST00000393096; ENSP00000376809; ENSG00000140093.

GeneID

51156.

KEGG

hsa:51156.

UCSC

uc001yct.1. human.

Organism-specific databases

CTD

51156.

GeneCards

GC14M094749.

H-InvDB

HIX0011928.

HGNC

HGNC:15996. SERPINA10.

MIM

602455. gene.
605271. gene+phenotype.

neXtProt

NX_Q9UK55.

PharmGKB

PA38078.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG13216.

GeneTree

ENSGT00560000076649.

HOGENOM

HBG714743.

HOVERGEN

HBG101138.

InParanoid

Q9UK55.

OMA

LLRKISM.

OrthoDB

EOG4VT5X7.

PhylomeDB

Q9UK55.

Gene expression databases

ArrayExpress

Q9UK55.

Bgee

Q9UK55.

CleanEx

HS_SERPINA10.

Genevestigator

Q9UK55.

GermOnline

ENSG00000140093. Homo sapiens.

Family and domain databases

InterPro

IPR000215. Protease_inhib_I4_serpin.
IPR023796. Sepin_dom.
[Graphical view]

PANTHER

PTHR11461. Prot_inh_serpin. 1 hit.

Pfam

PF00079. Serpin. 1 hit.
[Graphical view]

SMART

SM00093. SERPIN. 1 hit.
[Graphical view]

SUPFAM

SSF56574. Prot_inh_serpin. 1 hit.

PROSITE

PS00284. SERPIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

54061.

SOURCE

Search...

Entry information

Entry name

ZPI_HUMAN

Accession

Primary (citable) accession number: Q9UK55
Secondary accession number(s): A5Z2A5, Q6UWX9, Q86U20

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.