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Protein

Vacuolar protein sorting-associated protein 28 homolog

Gene

VPS28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.1 Publication

GO - Molecular functioni

  1. protein complex binding Source: GO_Central
  2. ubiquitin binding Source: UniProtKB

GO - Biological processi

  1. endosomal transport Source: Reactome
  2. intracellular transport of virus Source: Reactome
  3. membrane organization Source: Reactome
  4. negative regulation of protein ubiquitination Source: UniProtKB
  5. positive regulation of protein catabolic process Source: UniProtKB
  6. positive regulation of ubiquitin-dependent endocytosis Source: UniProtKB
  7. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  8. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
  9. viral life cycle Source: Reactome
  10. viral process Source: Reactome
  11. viral protein processing Source: Reactome
  12. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_115893. Membrane binding and targetting of GAG proteins.
REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_6359. Budding and maturation of HIV virion.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 28 homolog
Short name:
H-Vps28
Alternative name(s):
ESCRT-I complex subunit VPS28
Gene namesi
Name:VPS28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:18178. VPS28.

Subcellular locationi

Cell membrane 1 Publication. Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: MGI
  3. early endosome Source: UniProtKB
  4. endosome Source: MGI
  5. endosome membrane Source: UniProtKB
  6. ESCRT I complex Source: UniProtKB
  7. extracellular vesicular exosome Source: UniProtKB
  8. late endosome membrane Source: UniProtKB-SubCell
  9. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38512.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Vacuolar protein sorting-associated protein 28 homologPRO_0000120951Add
BLAST

Proteomic databases

MaxQBiQ9UK41.
PaxDbiQ9UK41.
PRIDEiQ9UK41.

PTM databases

PhosphoSiteiQ9UK41.

Expressioni

Gene expression databases

BgeeiQ9UK41.
CleanExiHS_VPS28.
ExpressionAtlasiQ9UK41. baseline and differential.
GenevestigatoriQ9UK41.

Organism-specific databases

HPAiHPA024688.
HPA024745.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoechiometry. Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TSG101Q998163EBI-727424,EBI-346882

Protein-protein interaction databases

BioGridi119341. 47 interactions.
IntActiQ9UK41. 16 interactions.
MINTiMINT-234404.
STRINGi9606.ENSP00000366565.

Structurei

3D structure databases

ProteinModelPortaliQ9UK41.
SMRiQ9UK41. Positions 24-117, 123-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 120108VPS28 N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini124 – 22097VPS28 C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the VPS28 family.PROSITE-ProRule annotation
Contains 1 VPS28 C-terminal domain.PROSITE-ProRule annotation
Contains 1 VPS28 N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG276841.
GeneTreeiENSGT00390000007486.
HOGENOMiHOG000203818.
HOVERGENiHBG054248.
InParanoidiQ9UK41.
KOiK12184.
OMAiKYDNMAD.
PhylomeDBiQ9UK41.
TreeFamiTF313364.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UK41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ
60 70 80 90 100
ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR
110 120 130 140 150
LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM
160 170 180 190 200
DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ
210 220
VRQMLFDLES AYNAFNRFLH A
Length:221
Mass (Da):25,425
Last modified:May 1, 2000 - v1
Checksum:iB2E1697B82D02AB8
GO
Isoform 2 (identifier: Q9UK41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-221: LQTLSGMSAS...YNAFNRFLHA → WVSLPARQSP...ADPERHVGVR

Note: No experimental confirmation available.

Show »
Length:233
Mass (Da):26,463
Checksum:i3EE28CC0503FCBF6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei184 – 22138LQTLS…RFLHA → WVSLPARQSPAVPETLPARR SPAVPLRPSAPTCPVLHSQA ADPERHVGVR in isoform 2. 1 PublicationVSP_042028Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182844 mRNA. Translation: AAF00499.1.
AF205589 Genomic DNA. No translation available.
BC006485 mRNA. Translation: AAH06485.1.
BC019321 mRNA. Translation: AAH19321.1.
BC050713 mRNA. Translation: AAH50713.1.
CCDSiCCDS34967.1. [Q9UK41-2]
CCDS6425.1. [Q9UK41-1]
RefSeqiNP_057292.1. NM_016208.3. [Q9UK41-1]
NP_898880.1. NM_183057.2. [Q9UK41-2]
UniGeneiHs.418175.

Genome annotation databases

EnsembliENST00000292510; ENSP00000292510; ENSG00000160948. [Q9UK41-1]
ENST00000377348; ENSP00000366565; ENSG00000160948. [Q9UK41-2]
ENST00000526054; ENSP00000434064; ENSG00000160948. [Q9UK41-1]
ENST00000529182; ENSP00000434556; ENSG00000160948. [Q9UK41-2]
GeneIDi51160.
KEGGihsa:51160.
UCSCiuc003zcs.1. human. [Q9UK41-1]
uc003zct.1. human. [Q9UK41-2]

Polymorphism databases

DMDMi13124619.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182844 mRNA. Translation: AAF00499.1.
AF205589 Genomic DNA. No translation available.
BC006485 mRNA. Translation: AAH06485.1.
BC019321 mRNA. Translation: AAH19321.1.
BC050713 mRNA. Translation: AAH50713.1.
CCDSiCCDS34967.1. [Q9UK41-2]
CCDS6425.1. [Q9UK41-1]
RefSeqiNP_057292.1. NM_016208.3. [Q9UK41-1]
NP_898880.1. NM_183057.2. [Q9UK41-2]
UniGeneiHs.418175.

3D structure databases

ProteinModelPortaliQ9UK41.
SMRiQ9UK41. Positions 24-117, 123-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119341. 47 interactions.
IntActiQ9UK41. 16 interactions.
MINTiMINT-234404.
STRINGi9606.ENSP00000366565.

PTM databases

PhosphoSiteiQ9UK41.

Polymorphism databases

DMDMi13124619.

Proteomic databases

MaxQBiQ9UK41.
PaxDbiQ9UK41.
PRIDEiQ9UK41.

Protocols and materials databases

DNASUi51160.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292510; ENSP00000292510; ENSG00000160948. [Q9UK41-1]
ENST00000377348; ENSP00000366565; ENSG00000160948. [Q9UK41-2]
ENST00000526054; ENSP00000434064; ENSG00000160948. [Q9UK41-1]
ENST00000529182; ENSP00000434556; ENSG00000160948. [Q9UK41-2]
GeneIDi51160.
KEGGihsa:51160.
UCSCiuc003zcs.1. human. [Q9UK41-1]
uc003zct.1. human. [Q9UK41-2]

Organism-specific databases

CTDi51160.
GeneCardsiGC08M145649.
HGNCiHGNC:18178. VPS28.
HPAiHPA024688.
HPA024745.
MIMi611952. gene.
neXtProtiNX_Q9UK41.
PharmGKBiPA38512.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG276841.
GeneTreeiENSGT00390000007486.
HOGENOMiHOG000203818.
HOVERGENiHBG054248.
InParanoidiQ9UK41.
KOiK12184.
OMAiKYDNMAD.
PhylomeDBiQ9UK41.
TreeFamiTF313364.

Enzyme and pathway databases

ReactomeiREACT_115893. Membrane binding and targetting of GAG proteins.
REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_6359. Budding and maturation of HIV virion.

Miscellaneous databases

ChiTaRSiVPS28. human.
GeneWikiiVPS28.
GenomeRNAii51160.
NextBioi54073.
PROiQ9UK41.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UK41.
CleanExiHS_VPS28.
ExpressionAtlasiQ9UK41. baseline and differential.
GenevestigatoriQ9UK41.

Family and domain databases

InterProiIPR007143. VPS28.
IPR017899. VPS28_C.
IPR017898. VPS28_N.
[Graphical view]
PANTHERiPTHR12937. PTHR12937. 1 hit.
PfamiPF03997. VPS28. 1 hit.
[Graphical view]
PIRSFiPIRSF017535. VPS28. 1 hit.
PROSITEiPS51310. VPS28_C. 1 hit.
PS51313. VPS28_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "H-vps28, a human homolog of yeast class E protein Vps28p localizes to an abnormal compartment in I-cell fibroblasts."
    Hunt P.R., Pevsner J.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Lung.
  4. "Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates."
    Bishop N., Horman A., Woodman P.
    J. Cell Biol. 157:91-101(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: INTERACTION WITH TSG101.
  6. "The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding."
    Stuchell M.D., Garrus J.E., Mueller B., Stray K.M., Ghaffarian S., McKinnon R., Kraeusslich H.-G., Morham S.G., Sundquist W.I.
    J. Biol. Chem. 279:36059-36071(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS37B.
  7. "Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding."
    Morita E., Sandrin V., Alam S.L., Eckert D.M., Gygi S.P., Sundquist W.I.
    Cell Host Microbe 2:41-53(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TSG101; VPS37B; VPS37C; MVB12A AND MVB12B, RECONSTITUTION OF THE ESCRT-I COMPLEX.
  8. "Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis."
    Morita E., Sandrin V., Chung H.Y., Morham S.G., Gygi S.P., Rodesch C.K., Sundquist W.I.
    EMBO J. 26:4215-4227(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CEP55.
  9. "Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex."
    Im Y.J., Hurley J.H.
    Dev. Cell 14:902-913(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VPS36; SNF8 AND VPS25.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting."
    Stefani F., Zhang L., Taylor S., Donovan J., Rollinson S., Doyotte A., Brownhill K., Bennion J., Pickering-Brown S., Woodman P.
    Curr. Biol. 21:1245-1250(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN AN ESCRT-I COMPLEX WITH UBAP1, SUBUNIT.

Entry informationi

Entry nameiVPS28_HUMAN
AccessioniPrimary (citable) accession number: Q9UK41
Secondary accession number(s): Q86VK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.