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Q9UK41

- VPS28_HUMAN

UniProt

Q9UK41 - VPS28_HUMAN

Protein

Vacuolar protein sorting-associated protein 28 homolog

Gene

VPS28

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Component of the ESCRT-I complex, a regulator of vesicular trafficking process.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. endosomal transport Source: Reactome
    2. intracellular transport of virus Source: Reactome
    3. membrane organization Source: Reactome
    4. negative regulation of protein ubiquitination Source: UniProtKB
    5. protein transport Source: UniProtKB-KW
    6. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
    7. viral life cycle Source: Reactome
    8. viral process Source: Reactome
    9. viral protein processing Source: Reactome
    10. virion assembly Source: Reactome

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_115893. Membrane binding and targetting of GAG proteins.
    REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    REACT_6359. Budding and maturation of HIV virion.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 28 homolog
    Short name:
    H-Vps28
    Alternative name(s):
    ESCRT-I complex subunit VPS28
    Gene namesi
    Name:VPS28
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:18178. VPS28.

    Subcellular locationi

    Cell membrane 1 Publication. Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: MGI
    3. endosome Source: MGI
    4. endosome membrane Source: Reactome
    5. ESCRT I complex Source: UniProtKB
    6. extracellular vesicular exosome Source: UniProt
    7. late endosome membrane Source: UniProtKB-SubCell
    8. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38512.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 221221Vacuolar protein sorting-associated protein 28 homologPRO_0000120951Add
    BLAST

    Proteomic databases

    MaxQBiQ9UK41.
    PaxDbiQ9UK41.
    PRIDEiQ9UK41.

    PTM databases

    PhosphoSiteiQ9UK41.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UK41.
    BgeeiQ9UK41.
    CleanExiHS_VPS28.
    GenevestigatoriQ9UK41.

    Organism-specific databases

    HPAiHPA024688.
    HPA024745.

    Interactioni

    Subunit structurei

    Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoechiometry. Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TSG101Q998163EBI-727424,EBI-346882

    Protein-protein interaction databases

    BioGridi119341. 15 interactions.
    IntActiQ9UK41. 14 interactions.
    MINTiMINT-234404.
    STRINGi9606.ENSP00000366565.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UK41.
    SMRiQ9UK41. Positions 24-117, 123-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 120108VPS28 N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini124 – 22097VPS28 C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the VPS28 family.PROSITE-ProRule annotation
    Contains 1 VPS28 C-terminal domain.PROSITE-ProRule annotation
    Contains 1 VPS28 N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG276841.
    HOGENOMiHOG000203818.
    HOVERGENiHBG054248.
    KOiK12184.
    OMAiFITIMDK.
    PhylomeDBiQ9UK41.
    TreeFamiTF313364.

    Family and domain databases

    InterProiIPR007143. VPS28.
    IPR017899. VPS28_C.
    IPR017898. VPS28_N.
    [Graphical view]
    PANTHERiPTHR12937. PTHR12937. 1 hit.
    PfamiPF03997. VPS28. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017535. VPS28. 1 hit.
    PROSITEiPS51310. VPS28_C. 1 hit.
    PS51313. VPS28_N. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UK41-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ    50
    ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR 100
    LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM 150
    DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ 200
    VRQMLFDLES AYNAFNRFLH A 221
    Length:221
    Mass (Da):25,425
    Last modified:May 1, 2000 - v1
    Checksum:iB2E1697B82D02AB8
    GO
    Isoform 2 (identifier: Q9UK41-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         184-221: LQTLSGMSAS...YNAFNRFLHA → WVSLPARQSP...ADPERHVGVR

    Note: No experimental confirmation available.

    Show »
    Length:233
    Mass (Da):26,463
    Checksum:i3EE28CC0503FCBF6
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei184 – 22138LQTLS…RFLHA → WVSLPARQSPAVPETLPARR SPAVPLRPSAPTCPVLHSQA ADPERHVGVR in isoform 2. 1 PublicationVSP_042028Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182844 mRNA. Translation: AAF00499.1.
    AF205589 Genomic DNA. No translation available.
    BC006485 mRNA. Translation: AAH06485.1.
    BC019321 mRNA. Translation: AAH19321.1.
    BC050713 mRNA. Translation: AAH50713.1.
    CCDSiCCDS34967.1. [Q9UK41-2]
    CCDS6425.1. [Q9UK41-1]
    RefSeqiNP_057292.1. NM_016208.3. [Q9UK41-1]
    NP_898880.1. NM_183057.2. [Q9UK41-2]
    UniGeneiHs.418175.

    Genome annotation databases

    EnsembliENST00000292510; ENSP00000292510; ENSG00000160948. [Q9UK41-1]
    ENST00000377348; ENSP00000366565; ENSG00000160948. [Q9UK41-2]
    ENST00000526054; ENSP00000434064; ENSG00000160948. [Q9UK41-1]
    ENST00000529182; ENSP00000434556; ENSG00000160948. [Q9UK41-2]
    GeneIDi51160.
    KEGGihsa:51160.
    UCSCiuc003zcs.1. human. [Q9UK41-1]
    uc003zct.1. human. [Q9UK41-2]

    Polymorphism databases

    DMDMi13124619.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182844 mRNA. Translation: AAF00499.1 .
    AF205589 Genomic DNA. No translation available.
    BC006485 mRNA. Translation: AAH06485.1 .
    BC019321 mRNA. Translation: AAH19321.1 .
    BC050713 mRNA. Translation: AAH50713.1 .
    CCDSi CCDS34967.1. [Q9UK41-2 ]
    CCDS6425.1. [Q9UK41-1 ]
    RefSeqi NP_057292.1. NM_016208.3. [Q9UK41-1 ]
    NP_898880.1. NM_183057.2. [Q9UK41-2 ]
    UniGenei Hs.418175.

    3D structure databases

    ProteinModelPortali Q9UK41.
    SMRi Q9UK41. Positions 24-117, 123-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119341. 15 interactions.
    IntActi Q9UK41. 14 interactions.
    MINTi MINT-234404.
    STRINGi 9606.ENSP00000366565.

    PTM databases

    PhosphoSitei Q9UK41.

    Polymorphism databases

    DMDMi 13124619.

    Proteomic databases

    MaxQBi Q9UK41.
    PaxDbi Q9UK41.
    PRIDEi Q9UK41.

    Protocols and materials databases

    DNASUi 51160.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000292510 ; ENSP00000292510 ; ENSG00000160948 . [Q9UK41-1 ]
    ENST00000377348 ; ENSP00000366565 ; ENSG00000160948 . [Q9UK41-2 ]
    ENST00000526054 ; ENSP00000434064 ; ENSG00000160948 . [Q9UK41-1 ]
    ENST00000529182 ; ENSP00000434556 ; ENSG00000160948 . [Q9UK41-2 ]
    GeneIDi 51160.
    KEGGi hsa:51160.
    UCSCi uc003zcs.1. human. [Q9UK41-1 ]
    uc003zct.1. human. [Q9UK41-2 ]

    Organism-specific databases

    CTDi 51160.
    GeneCardsi GC08M145649.
    HGNCi HGNC:18178. VPS28.
    HPAi HPA024688.
    HPA024745.
    MIMi 611952. gene.
    neXtProti NX_Q9UK41.
    PharmGKBi PA38512.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG276841.
    HOGENOMi HOG000203818.
    HOVERGENi HBG054248.
    KOi K12184.
    OMAi FITIMDK.
    PhylomeDBi Q9UK41.
    TreeFami TF313364.

    Enzyme and pathway databases

    Reactomei REACT_115893. Membrane binding and targetting of GAG proteins.
    REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    REACT_6359. Budding and maturation of HIV virion.

    Miscellaneous databases

    ChiTaRSi VPS28. human.
    GeneWikii VPS28.
    GenomeRNAii 51160.
    NextBioi 54073.
    PROi Q9UK41.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UK41.
    Bgeei Q9UK41.
    CleanExi HS_VPS28.
    Genevestigatori Q9UK41.

    Family and domain databases

    InterProi IPR007143. VPS28.
    IPR017899. VPS28_C.
    IPR017898. VPS28_N.
    [Graphical view ]
    PANTHERi PTHR12937. PTHR12937. 1 hit.
    Pfami PF03997. VPS28. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017535. VPS28. 1 hit.
    PROSITEi PS51310. VPS28_C. 1 hit.
    PS51313. VPS28_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "H-vps28, a human homolog of yeast class E protein Vps28p localizes to an abnormal compartment in I-cell fibroblasts."
      Hunt P.R., Pevsner J.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung.
    4. "Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates."
      Bishop N., Horman A., Woodman P.
      J. Cell Biol. 157:91-101(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: INTERACTION WITH TSG101.
    6. "The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding."
      Stuchell M.D., Garrus J.E., Mueller B., Stray K.M., Ghaffarian S., McKinnon R., Kraeusslich H.-G., Morham S.G., Sundquist W.I.
      J. Biol. Chem. 279:36059-36071(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VPS37B.
    7. "Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding."
      Morita E., Sandrin V., Alam S.L., Eckert D.M., Gygi S.P., Sundquist W.I.
      Cell Host Microbe 2:41-53(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TSG101; VPS37B; VPS37C; MVB12A AND MVB12B, RECONSTITUTION OF THE ESCRT-I COMPLEX.
    8. "Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis."
      Morita E., Sandrin V., Chung H.Y., Morham S.G., Gygi S.P., Rodesch C.K., Sundquist W.I.
      EMBO J. 26:4215-4227(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CEP55.
    9. "Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex."
      Im Y.J., Hurley J.H.
      Dev. Cell 14:902-913(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VPS36; SNF8 AND VPS25.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting."
      Stefani F., Zhang L., Taylor S., Donovan J., Rollinson S., Doyotte A., Brownhill K., Bennion J., Pickering-Brown S., Woodman P.
      Curr. Biol. 21:1245-1250(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN ESCRT-I COMPLEX WITH UBAP1, SUBUNIT.

    Entry informationi

    Entry nameiVPS28_HUMAN
    AccessioniPrimary (citable) accession number: Q9UK41
    Secondary accession number(s): Q86VK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3