Q9UK41 (VPS28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 28 homolog
Short name=H-Vps28

Alternative name(s):
ESCRT-I complex subunit VPS28

Gene names

Name:

VPS28

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Ref.4
Subunit structure	Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Cell membrane. Late endosome membrane; Peripheral membrane protein Ref.4.
Sequence similarities	Belongs to the VPS28 family. Contains 1 VPS28 C-terminal domain. Contains 1 VPS28 N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Endosome Membrane
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane organization Traceable author statement. Source: Reactome egress of virus within host cell Traceable author statement. Source: Reactome endosomal transport Traceable author statement. Source: Reactome negative regulation of protein ubiquitination Inferred from direct assay PubMed 18077552. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW viral protein processing Traceable author statement. Source: Reactome virion assembly Traceable author statement. Source: Reactome
Cellular_component	cytosol Inferred from direct assay PubMed 11134028. Source: MGI endosome membrane Traceable author statement. Source: Reactome late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TSG101	Q99816	3	EBI-727424,EBI-346882

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UK41-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UK41-2)

The sequence of this isoform differs from the canonical sequence as follows:
     184-221: LQTLSGMSAS...YNAFNRFLHA → WVSLPARQSP...ADPERHVGVR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Vacuolar protein sorting-associated protein 28 homolog		PRO_0000120951
Regions
	Domain	13 – 120	108	VPS28 N-terminal
	Domain	124 – 220	97	VPS28 C-terminal
Natural variations
	Alternative sequence	184 – 221	38	LQTLS…RFLHA → WVSLPARQSPAVPETLPARR SPAVPLRPSAPTCPVLHSQA ADPERHVGVR in isoform 2.		VSP_042028

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B2E1697B82D02AB8	FASTA	221	25,425
	10 20 30 40 50 60 MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC 70 80 90 100 110 120 VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD 130 140 150 160 170 180 DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV 190 200 210 220 SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A
	Isoform 2 [UniParc]. Checksum: 3EE28CC0503FCBF6	FASTA	233	26,463
	10 20 30 40 50 60 MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC 70 80 90 100 110 120 VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD 130 140 150 160 170 180 DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV 190 200 210 220 230 SQWWVSLPAR QSPAVPETLP ARRSPAVPLR PSAPTCPVLH SQAADPERHV GVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"H-vps28, a human homolog of yeast class E protein Vps28p localizes to an abnormal compartment in I-cell fibroblasts." Hunt P.R., Pevsner J. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[4]	"Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates." Bishop N., Horman A., Woodman P. J. Cell Biol. 157:91-101(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"The protein network of HIV budding." von Schwedler U.K., Stuchell M., Mueller B., Ward D.M., Chung H.-Y., Morita E., Wang H.E., Davis T., He G.P., Cimbora D.M., Scott A., Kraeusslich H.-G., Kaplan J., Morham S.G., Sundquist W.I. Cell 114:701-713(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TSG101.
[6]	"The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding." Stuchell M.D., Garrus J.E., Mueller B., Stray K.M., Ghaffarian S., McKinnon R., Kraeusslich H.-G., Morham S.G., Sundquist W.I. J. Biol. Chem. 279:36059-36071(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VPS37B.
[7]	"Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding." Morita E., Sandrin V., Alam S.L., Eckert D.M., Gygi S.P., Sundquist W.I. Cell Host Microbe 2:41-53(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TSG101; VPS37B; VPS37C; MVB12A AND MVB12B, RECONSTITUTION OF THE ESCRT-I COMPLEX.
[8]	"Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis." Morita E., Sandrin V., Chung H.Y., Morham S.G., Gygi S.P., Rodesch C.K., Sundquist W.I. EMBO J. 26:4215-4227(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CEP55.
[9]	"Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex." Im Y.J., Hurley J.H. Dev. Cell 14:902-913(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VPS36; SNF8 AND VPS25.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF182844 mRNA. Translation: AAF00499.1. AF205589 Genomic DNA. No translation available. BC006485 mRNA. Translation: AAH06485.1. BC019321 mRNA. Translation: AAH19321.1. BC050713 mRNA. Translation: AAH50713.1.
IPI	IPI00007155.
RefSeq	NP_057292.1. NM_016208.2. NP_898880.1. NM_183057.1.
UniGene	Hs.418175.
3D structure databases
ProteinModelPortal	Q9UK41.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UK41. 7 interactions.
MINT	MINT-234404.
STRING	9606.ENSP00000366565.
PTM databases
PhosphoSite	Q9UK41.
Polymorphism databases
DMDM	13124619.
Proteomic databases
PaxDb	Q9UK41.
PRIDE	Q9UK41.
Protocols and materials databases
DNASU	51160.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000292510; ENSP00000292510; ENSG00000160948. ENST00000377348; ENSP00000366565; ENSG00000160948. ENST00000526054; ENSP00000434064; ENSG00000160948. ENST00000529182; ENSP00000434556; ENSG00000160948. ENST00000562786; ENSP00000457919; ENSG00000260313. ENST00000565984; ENSP00000456843; ENSG00000260313.
GeneID	51160.
KEGG	hsa:51160.
UCSC	uc003zcs.1. human. uc003zct.1. human.
Organism-specific databases
CTD	51160.
GeneCards	GC08M145649.
HGNC	HGNC:18178. VPS28.
HPA	HPA024688. HPA024745.
MIM	611952. gene.
neXtProt	NX_Q9UK41.
PharmGKB	PA38512.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG276841.
HOGENOM	HOG000203818.
HOVERGEN	HBG054248.
KO	K12184.
OMA	FITIMDK.
OrthoDB	EOG4TQMB1.
PhylomeDB	Q9UK41.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking. REACT_116125. Disease.
Gene expression databases
ArrayExpress	Q9UK41.
Bgee	Q9UK41.
CleanEx	HS_VPS28.
Genevestigator	Q9UK41.
GermOnline	ENSG00000160948. Homo sapiens.
Family and domain databases
InterPro	IPR007143. VPS28. IPR017899. VPS28_C. IPR017898. VPS28_N. [Graphical view]
PANTHER	PTHR12937. PTHR12937. 1 hit.
Pfam	PF03997. VPS28. 1 hit. [Graphical view]
PIRSF	PIRSF017535. VPS28. 1 hit.
PROSITE	PS51310. VPS28_C. 1 hit. PS51313. VPS28_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	VPS28. human.
GenomeRNAi	51160.
NextBio	54073.
SOURCE	Search...

Entry information

Entry name

VPS28_HUMAN

Accession

Primary (citable) accession number: Q9UK41
Secondary accession number(s): Q86VK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.