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Protein

Nocturnin

Gene

NOCT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Circadian deadenylase which plays an important role in post-transcriptional regulation of metabolic genes under circadian control. Degrades poly(A) tails of specific target mRNAs leading to their degradation and suppression of translation. Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis. Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance. Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation. Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone. Critical for proper development of early embryos (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei195 – 1951By similarity
Metal bindingi195 – 1951Magnesium 1By similarity
Metal bindingi324 – 3241Magnesium 2By similarity
Metal bindingi326 – 3261Magnesium 2By similarity
Active sitei377 – 3771By similarity
Active sitei414 – 4141By similarity
Metal bindingi414 – 4141Magnesium 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Biological rhythms, mRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-1368108. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
NocturninBy similarityImported (EC:3.1.13.4)
Alternative name(s):
Carbon catabolite repression 4-like protein
Circadian deadenylase NOC
Gene namesi
Name:NOCTImported
Synonyms:CCR4, CCRN4L, NOC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:14254. NOCT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26176.

Polymorphism and mutation databases

DMDMi212276446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431NocturninPRO_0000218568Add
BLAST

Proteomic databases

EPDiQ9UK39.
MaxQBiQ9UK39.
PaxDbiQ9UK39.
PeptideAtlasiQ9UK39.
PRIDEiQ9UK39.

PTM databases

iPTMnetiQ9UK39.
PhosphoSiteiQ9UK39.

Expressioni

Tissue specificityi

Adipose tissue. Expression is higher in subcutaneous adipose tissue as compared to visceral adipose tissue.1 Publication

Gene expression databases

BgeeiQ9UK39.
CleanExiHS_CCR4.
HS_CCRN4L.
ExpressionAtlasiQ9UK39. baseline and differential.
GenevisibleiQ9UK39. HS.

Organism-specific databases

HPAiHPA012287.
HPA053714.

Interactioni

Subunit structurei

Interacts with PPARG.By similarity

Protein-protein interaction databases

BioGridi117347. 28 interactions.
IntActiQ9UK39. 2 interactions.
STRINGi9606.ENSP00000280614.

Structurei

3D structure databases

ProteinModelPortaliQ9UK39.
SMRiQ9UK39. Positions 145-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 35311Interaction with PPARGBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000237357.
HOVERGENiHBG006512.
InParanoidiQ9UK39.
KOiK18764.
OMAiMFQSPRR.
OrthoDBiEOG7Z95MC.
PhylomeDBiQ9UK39.
TreeFamiTF323175.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UK39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHSPRRLCS ALLQRDAPGL RRLPAPGLRR PLSPPAAVPR PASPRLLAAA
60 70 80 90 100
SAASGAARSC SRTVCSMGTG TSRLYSALAK TLNSSAASQH PEYLVSPDPE
110 120 130 140 150
HLEPIDPKEL LEECRAVLHT RPPRFQRDFV DLRTDCPSTH PPIRVMQWNI
160 170 180 190 200
LAQALGEGKD NFVQCPVEAL KWEERKCLIL EEILAYQPDI LCLQEVDHYF
210 220 230 240 250
DTFQPLLSRL GYQGTFFPKP WSPCLDVEHN NGPDGCALFF LQNRFKLVNS
260 270 280 290 300
ANIRLTAMTL KTNQVAIAQT LECKESGRQF CIAVTHLKAR TGWERFRSAQ
310 320 330 340 350
GCDLLQNLQN ITQGAKIPLI VCGDFNAEPT EEVYKHFASS SLNLNSAYKL
360 370 380 390 400
LSADGQSEPP YTTWKIRTSG ECRHTLDYIW YSKHALNVRS ALDLLTEEQI
410 420 430
GPNRLPSFNY PSDHLSLVCD FSFTEESDGL S
Length:431
Mass (Da):48,196
Last modified:November 4, 2008 - v2
Checksum:i5BDEFC43173B2D0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161D → G in AAD56548 (PubMed:10521507).Curated
Sequence conflicti69 – 691T → N in AAG01387 (Ref. 4) Curated
Sequence conflicti77 – 771A → G in AAD56548 (PubMed:10521507).Curated
Sequence conflicti266 – 2661A → T in AAG01389 (Ref. 4) Curated
Sequence conflicti341 – 3411S → N in AAD56548 (PubMed:10521507).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti140 – 1401H → Y.
Corresponds to variant rs2271777 [ dbSNP | Ensembl ].
VAR_047096

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF183961 mRNA. Translation: AAD56548.1.
CH471056 Genomic DNA. Translation: EAX05129.1.
CH471056 Genomic DNA. Translation: EAX05131.1.
BC113494 mRNA. Translation: AAI13495.1.
BC113500 mRNA. Translation: AAI13501.1.
AF199492 Genomic DNA. Translation: AAG01387.1.
AF199493 mRNA. Translation: AAG01388.1.
AF199494 Transcribed RNA. Translation: AAG01389.1.
CCDSiCCDS3743.1.
RefSeqiNP_036250.2. NM_012118.3.
UniGeneiHs.639842.
Hs.656047.

Genome annotation databases

EnsembliENST00000280614; ENSP00000280614; ENSG00000151014.
GeneIDi25819.
KEGGihsa:25819.
UCSCiuc003ihl.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF183961 mRNA. Translation: AAD56548.1.
CH471056 Genomic DNA. Translation: EAX05129.1.
CH471056 Genomic DNA. Translation: EAX05131.1.
BC113494 mRNA. Translation: AAI13495.1.
BC113500 mRNA. Translation: AAI13501.1.
AF199492 Genomic DNA. Translation: AAG01387.1.
AF199493 mRNA. Translation: AAG01388.1.
AF199494 Transcribed RNA. Translation: AAG01389.1.
CCDSiCCDS3743.1.
RefSeqiNP_036250.2. NM_012118.3.
UniGeneiHs.639842.
Hs.656047.

3D structure databases

ProteinModelPortaliQ9UK39.
SMRiQ9UK39. Positions 145-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117347. 28 interactions.
IntActiQ9UK39. 2 interactions.
STRINGi9606.ENSP00000280614.

PTM databases

iPTMnetiQ9UK39.
PhosphoSiteiQ9UK39.

Polymorphism and mutation databases

DMDMi212276446.

Proteomic databases

EPDiQ9UK39.
MaxQBiQ9UK39.
PaxDbiQ9UK39.
PeptideAtlasiQ9UK39.
PRIDEiQ9UK39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000280614; ENSP00000280614; ENSG00000151014.
GeneIDi25819.
KEGGihsa:25819.
UCSCiuc003ihl.5. human.

Organism-specific databases

CTDi25819.
GeneCardsiCCRN4L.
H-InvDBHIX0004503.
HGNCiHGNC:14254. NOCT.
HPAiHPA012287.
HPA053714.
MIMi608468. gene.
neXtProtiNX_Q9UK39.
PharmGKBiPA26176.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000237357.
HOVERGENiHBG006512.
InParanoidiQ9UK39.
KOiK18764.
OMAiMFQSPRR.
OrthoDBiEOG7Z95MC.
PhylomeDBiQ9UK39.
TreeFamiTF323175.

Enzyme and pathway databases

ReactomeiR-HSA-1368108. BMAL1:CLOCK,NPAS2 activates circadian gene expression.

Miscellaneous databases

ChiTaRSiCCRN4L. human.
GenomeRNAii25819.
PROiQ9UK39.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UK39.
CleanExiHS_CCR4.
HS_CCRN4L.
ExpressionAtlasiQ9UK39. baseline and differential.
GenevisibleiQ9UK39. HS.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a mammalian gene related to the yeast CCR4 general transcription factor and revealed by transposon insertion."
    Dupressoir A., Barbot W., Loireau M.-P., Heidmann T.
    J. Biol. Chem. 274:31068-31075(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Mammalian homologs of Xenopus nocturnin: conservation of structure and circadian regulation."
    Wang Y., Osterbur D.L., Green C.B., Besharse J.C.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 67-431.
  5. "The role of nocturnin in early adipogenesis and modulation of systemic insulin resistance in human."
    Hee S.W., Tsai S.H., Chang Y.C., Chang C.J., Yu I.S., Lee P.C., Lee W.J., Yun-Chia Chang E., Chuang L.M.
    Obesity 20:1558-1565(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Nocturnin: at the crossroads of clocks and metabolism."
    Stubblefield J.J., Terrien J., Green C.B.
    Trends Endocrinol. Metab. 23:326-333(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiNOCT_HUMAN
AccessioniPrimary (citable) accession number: Q9UK39
Secondary accession number(s): D3DNY5
, Q14D51, Q9HD93, Q9HD94, Q9HD95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 4, 2008
Last modified: July 6, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.