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Protein

ADP-ribosylation factor-binding protein GGA2

Gene

GGA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103365-MONOMER.
ReactomeiR-HSA-977225. Amyloid fiber formation.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-binding protein GGA2
Alternative name(s):
Gamma-adaptin-related protein 2
Golgi-localized, gamma ear-containing, ARF-binding protein 2
VHS domain and ear domain of gamma-adaptin
Short name:
Vear
Gene namesi
Name:GGA2
Synonyms:KIAA1080
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:16064. GGA2.

Subcellular locationi

GO - Cellular componenti

  • clathrin adaptor complex Source: InterPro
  • clathrin-coated vesicle Source: UniProtKB
  • endosome membrane Source: Reactome
  • Golgi apparatus Source: HPA
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi349 – 353LIDLE → AADAA: Partial loss of clathrin-binding. 1 Publication5

Organism-specific databases

OpenTargetsiENSG00000103365.
PharmGKBiPA28658.

Polymorphism and mutation databases

BioMutaiGGA2.
DMDMi143811397.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126821 – 613ADP-ribosylation factor-binding protein GGA2Add BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei400PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9UJY4.
MaxQBiQ9UJY4.
PaxDbiQ9UJY4.
PeptideAtlasiQ9UJY4.
PRIDEiQ9UJY4.
TopDownProteomicsiQ9UJY4.

PTM databases

iPTMnetiQ9UJY4.
PhosphoSitePlusiQ9UJY4.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSG00000103365.
CleanExiHS_GGA2.
ExpressionAtlasiQ9UJY4. baseline and differential.
GenevisibleiQ9UJY4. HS.

Organism-specific databases

HPAiCAB034422.
HPA043313.
HPA063634.

Interactioni

Subunit structurei

Monomer. Interacts with NECAP1, TSG101, UBC and AFTPH/aftiphilin. Interacts with CNST (By similarity). Interacts with GGA1 and GGA3. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds the accessory proteins CCDC91, P200, SYNRG, EPN4 and NECAP2.By similarity14 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CA8P352193EBI-447646,EBI-718700
RABEP1Q152766EBI-447646,EBI-447043
SPG21Q9NZD83EBI-447646,EBI-742688

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116697. 34 interactors.
DIPiDIP-31601N.
IntActiQ9UJY4. 26 interactors.
MINTiMINT-126110.
STRINGi9606.ENSP00000311962.

Structurei

Secondary structure

1613
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 34Combined sources9
Helixi43 – 55Combined sources13
Helixi58 – 71Combined sources14
Helixi76 – 92Combined sources17
Helixi95 – 101Combined sources7
Helixi104 – 112Combined sources9
Helixi125 – 141Combined sources17
Helixi146 – 157Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MHQX-ray2.20A/B25-172[»]
ProteinModelPortaliQ9UJY4.
SMRiQ9UJY4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UJY4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 163VHSPROSITE-ProRule annotationAdd BLAST131
Domaini188 – 315GATPROSITE-ProRule annotationAdd BLAST128
Domaini484 – 605GAEPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni316 – 483Unstructured hingeAdd BLAST168

Domaini

The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (AC-LL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.

Sequence similaritiesi

Belongs to the GGA protein family.Curated
Contains 1 GAE domain.PROSITE-ProRule annotation
Contains 1 GAT domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00860000133703.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ9UJY4.
KOiK12404.
OMAiKVAGQNC.
OrthoDBiEOG091G0BQK.
PhylomeDBiQ9UJY4.
TreeFamiTF318574.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UJY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATAVAAAV AGTESAQGPP GPAASLELWL NKATDPSMSE QDWSAIQNFC
60 70 80 90 100
EQVNTDPNGP THAPWLLAHK IQSPQEKEAL YALTVLEMCM NHCGEKFHSE
110 120 130 140 150
VAKFRFLNEL IKVLSPKYLG SWATGKVKGR VIEILFSWTV WFPEDIKIRD
160 170 180 190 200
AYQMLKKQGI IKQDPKLPVD KILPPPSPWP KSSIFDADEE KSKLLTRLLK
210 220 230 240 250
SNHPEDLQAA NRLIKNLVKE EQEKSEKVSK RVSAVEEVRS HVKVLQEMLS
260 270 280 290 300
MYRRPGQAPP DQEALQVVYE RCEKLRPTLF RLASDTTDDD DALAEILQAN
310 320 330 340 350
DLLTQGVLLY KQVMEGRVTF GNRVTSSLGD IPVSRVFQNP AGCMKTCPLI
360 370 380 390 400
DLEVDNGPAQ MGTVVPSLLH QDLAALGISD APVTGMVSGQ NCCEEKRNPS
410 420 430 440 450
SSTLPGGGVQ NPSADRNLLD LLSAQPAPCP LNYVSQKSVP KEVPPGTKSS
460 470 480 490 500
PGWSWEAGPL APSPSSQNTP LAQVFVPLES VKPSSLPPLI VYDRNGFRIL
510 520 530 540 550
LHFSQTGAPG HPEVQVLLLT MMSTAPQPVW DIMFQVAVPK SMRVKLQPAS
560 570 580 590 600
SSKLPAFSPL MPPAVISQML LLDNPHKEPI RLRYKLTFNQ GGQPFSEVGE
610
VKDFPDLAVL GAA
Length:613
Mass (Da):67,150
Last modified:April 3, 2007 - v3
Checksum:i4B627ABA474A069C
GO

Sequence cautioni

The sequence AAK38634 differs from that shown. Reason: Frameshift at position 193.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281R → W in AAF05708 (PubMed:10749927).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028275424A → P.4 PublicationsCorresponds to variant rs1135045dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190863 mRNA. Translation: AAF05708.1.
AF233522 mRNA. Translation: AAF35394.1.
AF165531 mRNA. Translation: AAF42806.1.
AC002400 Genomic DNA. Translation: AAC05813.1.
CH471145 Genomic DNA. Translation: EAW55827.1.
CH471145 Genomic DNA. Translation: EAW55828.1.
BC000284 mRNA. Translation: AAH00284.1.
AF323754 mRNA. Translation: AAK38634.1. Frameshift.
AB029003 mRNA. Translation: BAA83032.1.
CCDSiCCDS10611.1.
PIRiT00744.
RefSeqiNP_055859.1. NM_015044.4.
UniGeneiHs.460336.

Genome annotation databases

EnsembliENST00000309859; ENSP00000311962; ENSG00000103365.
GeneIDi23062.
KEGGihsa:23062.
UCSCiuc002dlq.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190863 mRNA. Translation: AAF05708.1.
AF233522 mRNA. Translation: AAF35394.1.
AF165531 mRNA. Translation: AAF42806.1.
AC002400 Genomic DNA. Translation: AAC05813.1.
CH471145 Genomic DNA. Translation: EAW55827.1.
CH471145 Genomic DNA. Translation: EAW55828.1.
BC000284 mRNA. Translation: AAH00284.1.
AF323754 mRNA. Translation: AAK38634.1. Frameshift.
AB029003 mRNA. Translation: BAA83032.1.
CCDSiCCDS10611.1.
PIRiT00744.
RefSeqiNP_055859.1. NM_015044.4.
UniGeneiHs.460336.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MHQX-ray2.20A/B25-172[»]
ProteinModelPortaliQ9UJY4.
SMRiQ9UJY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116697. 34 interactors.
DIPiDIP-31601N.
IntActiQ9UJY4. 26 interactors.
MINTiMINT-126110.
STRINGi9606.ENSP00000311962.

PTM databases

iPTMnetiQ9UJY4.
PhosphoSitePlusiQ9UJY4.

Polymorphism and mutation databases

BioMutaiGGA2.
DMDMi143811397.

Proteomic databases

EPDiQ9UJY4.
MaxQBiQ9UJY4.
PaxDbiQ9UJY4.
PeptideAtlasiQ9UJY4.
PRIDEiQ9UJY4.
TopDownProteomicsiQ9UJY4.

Protocols and materials databases

DNASUi23062.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309859; ENSP00000311962; ENSG00000103365.
GeneIDi23062.
KEGGihsa:23062.
UCSCiuc002dlq.3. human.

Organism-specific databases

CTDi23062.
GeneCardsiGGA2.
HGNCiHGNC:16064. GGA2.
HPAiCAB034422.
HPA043313.
HPA063634.
MIMi606005. gene.
neXtProtiNX_Q9UJY4.
OpenTargetsiENSG00000103365.
PharmGKBiPA28658.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1087. Eukaryota.
ENOG410Y26G. LUCA.
GeneTreeiENSGT00860000133703.
HOGENOMiHOG000231169.
HOVERGENiHBG015945.
InParanoidiQ9UJY4.
KOiK12404.
OMAiKVAGQNC.
OrthoDBiEOG091G0BQK.
PhylomeDBiQ9UJY4.
TreeFamiTF318574.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000103365-MONOMER.
ReactomeiR-HSA-977225. Amyloid fiber formation.

Miscellaneous databases

ChiTaRSiGGA2. human.
EvolutionaryTraceiQ9UJY4.
GeneWikiiGGA2.
GenomeRNAii23062.
PROiQ9UJY4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103365.
CleanExiHS_GGA2.
ExpressionAtlasiQ9UJY4. baseline and differential.
GenevisibleiQ9UJY4. HS.

Family and domain databases

Gene3Di1.20.58.160. 1 hit.
1.25.40.90. 1 hit.
2.60.40.1230. 1 hit.
InterProiIPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
IPR008942. ENTH_VHS.
IPR004152. GAT_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF02883. Alpha_adaptinC2. 1 hit.
PF03127. GAT. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
PS50909. GAT. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGA2_HUMAN
AccessioniPrimary (citable) accession number: Q9UJY4
Secondary accession number(s): D3DWF0
, O14564, Q9NYN2, Q9UPS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: April 3, 2007
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.