Reviewed,
UniProtKB/Swiss-Prot Q9UJX5 (APC4_HUMAN)
Last modified
December 15, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Anaphase-promoting complex subunit 4 Short name=APC4 Alternative name(s): Cyclosome subunit 4 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 808 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
| Pathway | |
| Subunit structure | The APC/C is composed of at least 12 subunits. |
| Sequence similarities | Belongs to the APC4 family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9UJX5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9UJX5-2) The sequence of this isoform differs from the canonical sequence as follows: 542-551: DVIGKSMNQA → VSLKEMHVFV 552-808: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 808 | 808 | Anaphase-promoting complex subunit 4 | PRO_0000064595 | |||||
Amino acid modifications | |||||||||
| Modified residue | 469 | 1 | Phosphotyrosine Ref.6 | ||||||
| Modified residue | 757 | 1 | Phosphoserine | ||||||
| Modified residue | 758 | 1 | Phosphoserine | ||||||
| Modified residue | 777 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 779 | 1 | Phosphoserine Ref.6 Ref.8 | ||||||
Natural variations | |||||||||
| Alternative sequence | 542 – 551 | 10 | DVIGKSMNQA → VSLKEMHVFV in isoform 2. | VSP_008464 | |||||
| Alternative sequence | 552 – 808 | 257 | Missing in isoform 2. | VSP_008465 | |||||
| Natural variant | 155 | 1 | I → V in a colorectal cancer sample; somatic mutation. Ref.12 | VAR_035792 | |||||
| Natural variant | 465 | 1 | R → Q: dbSNP rs34811474. | VAR_054044 | |||||
| Natural variant | 800 | 1 | E → G: dbSNP rs11550697. | VAR_054045 | |||||
Experimental info | |||||||||
| Sequence conflict | 286 | 1 | R → C in AAF05752. Ref.1 | ||||||
| Sequence conflict | 293 – 295 | 3 | EKN → GKD in AAF05752. Ref.1 | ||||||
| Sequence conflict | 756 | 1 | E → G in AAH59383. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a cullin homology region in a subunit of the anaphase-promoting complex." Yu H., Peters J.-M., King R.W., Page A.M., Hieter P., Kirschner M.W. Science 279:1219-1222(1998) [PubMed: 9469815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta. |
| [5] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 434-808 (ISOFORM 2). Tissue: Amygdala. |
| [6] | "Mitotic regulation of the human anaphase-promoting complex by phosphorylation." Kraft C., Herzog F., Gieffers C., Mechtler K., Hagting A., Pines J., Peters J.-M. EMBO J. 22:6598-6609(2003) [PubMed: 14657031] [Abstract] Cited for: PHOSPHORYLATION AT TYR-469 AND SER-779. |
| [7] | "Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex." Jin L., Williamson A., Banerjee S., Philipp I., Rape M. Cell 133:653-665(2008) [PubMed: 18485873] [Abstract] Cited for: FUNCTION OF THE APC/C. |
| [8] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-777 AND SER-779, MASS SPECTROMETRY. |
| [9] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-779, MASS SPECTROMETRY. |
| [10] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-757 AND SER-758, MASS SPECTROMETRY. Tissue: T-cell. |
| [11] | "Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C." Dube P., Herzog F., Gieffers C., Sander B., Riedel D., Mueller S.A., Engel A., Peters J.-M., Stark H. Mol. Cell 20:867-879(2005) [PubMed: 16364912] [Abstract] Cited for: ELECTRON MICROSCOPY OF THE APC/C. |
| [12] | "The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-155. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF191338 mRNA. Translation: AAF05752.1. AK292336 mRNA. Translation: BAF85025.1. CH471069 Genomic DNA. Translation: EAW92839.1. BC059383 mRNA. Translation: AAH59383.1. AL353932 mRNA. Translation: CAB89245.1. | |
| IPI | IPI00002551. IPI00419201. |
| PIR | T48682. |
| RefSeq | NP_037499.2. |
| UniGene | Hs.152173 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9UJX5. |
PTM databases | |
| PhosphoSite | Q9UJX5. |
Proteomic databases | |
| PRIDE | Q9UJX5. |
Genome annotation databases | |
| Ensembl | ENST00000315368; ENSP00000318775; ENSG00000053900; Homo sapiens. [Genome view] |
| GeneID | 29945. |
| KEGG | hsa:29945. |
Organism-specific databases | |
| CTD | 29945. |
| GeneCards | GC04P025055. |
| H-InvDB | HIX0020484. |
| HGNC | HGNC:19990. ANAPC4. |
| MIM | 606947. gene. |
| PharmGKB | PA134894250. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG714578. |
| HOVERGEN | Q9UJX5. |
| InParanoid | Q9UJX5. |
| OMA | FVQEKNT. |
| OrthoDB | EOG9VQD8R. |
Enzyme and pathway databases | |
| Reactome | REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_8017. APC-Cdc20 mediated degradation of Nek2A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| ArrayExpress | Q9UJX5. |
| Bgee | Q9UJX5. |
| CleanEx | HS_ANAPC4. |
| Genevestigator | Q9UJX5. |
| GermOnline | ENSG00000053900. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR017169. APC4. IPR015943. WD40/YVTN_repeat-like_dom. IPR011046. WD40_repeat-like_dom. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| PIRSF | PIRSF037303. APC4. 1 hit. |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | APC4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9UJX5 Secondary accession number(s): A8K8H1, Q6PCC6, Q9NSH6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 4 Human chromosome 4: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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