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Protein

Anaphase-promoting complex subunit 5

Gene

ANAPC5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein phosphatase binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiQ9UJX4
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 5
Short name:
APC5
Alternative name(s):
Cyclosome subunit 5
Gene namesi
Name:ANAPC5
Synonyms:APC5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000089053.12
HGNCiHGNC:15713 ANAPC5
MIMi606948 gene
neXtProtiNX_Q9UJX4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi51433
OpenTargetsiENSG00000089053
PharmGKBiPA24788

Polymorphism and mutation databases

BioMutaiANAPC5
DMDMi37537861

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000645971 – 755Anaphase-promoting complex subunit 5Add BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195Phosphoserine1 Publication1
Modified residuei232PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UJX4
PaxDbiQ9UJX4
PeptideAtlasiQ9UJX4
PRIDEiQ9UJX4
ProteomicsDBi84684
84685 [Q9UJX4-2]

PTM databases

iPTMnetiQ9UJX4
PhosphoSitePlusiQ9UJX4

Expressioni

Gene expression databases

BgeeiENSG00000089053
CleanExiHS_ANAPC5
ExpressionAtlasiQ9UJX4 baseline and differential
GenevisibleiQ9UJX4 HS

Organism-specific databases

HPAiHPA039457
HPA039801

Interactioni

Subunit structurei

The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.2 Publications

GO - Molecular functioni

  • protein phosphatase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi119537, 78 interactors
CORUMiQ9UJX4
DIPiDIP-32945N
IntActiQ9UJX4, 39 interactors
MINTiQ9UJX4
STRINGi9606.ENSP00000261819

Structurei

Secondary structure

1755
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 44Combined sources13
Turni47 – 49Combined sources3
Helixi54 – 69Combined sources16
Turni75 – 77Combined sources3
Helixi78 – 85Combined sources8
Helixi87 – 102Combined sources16
Helixi105 – 121Combined sources17
Helixi130 – 145Combined sources16
Helixi148 – 166Combined sources19
Helixi209 – 225Combined sources17
Beta strandi227 – 230Combined sources4
Helixi233 – 246Combined sources14
Helixi252 – 264Combined sources13
Helixi267 – 280Combined sources14
Turni289 – 291Combined sources3
Helixi298 – 314Combined sources17
Helixi317 – 334Combined sources18
Helixi337 – 350Combined sources14
Turni351 – 353Combined sources3
Helixi361 – 369Combined sources9
Helixi374 – 390Combined sources17
Helixi403 – 411Combined sources9
Helixi415 – 431Combined sources17
Helixi435 – 447Combined sources13
Helixi463 – 479Combined sources17
Helixi483 – 496Combined sources14
Helixi504 – 522Combined sources19
Helixi525 – 539Combined sources15
Helixi541 – 554Combined sources14
Helixi557 – 574Combined sources18
Helixi577 – 593Combined sources17
Helixi597 – 599Combined sources3
Helixi600 – 614Combined sources15
Helixi617 – 633Combined sources17
Helixi637 – 653Combined sources17
Helixi658 – 677Combined sources20
Helixi683 – 704Combined sources22
Helixi708 – 724Combined sources17
Helixi727 – 742Combined sources16
Turni750 – 754Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UI9electron microscopy3.60O1-755[»]
5A31electron microscopy4.30O1-755[»]
5G04electron microscopy4.00O1-755[»]
5G05electron microscopy3.40O1-755[»]
5KHRelectron microscopy6.10O1-755[»]
5KHUelectron microscopy4.80O1-755[»]
5L9Telectron microscopy6.40O1-755[»]
5L9Uelectron microscopy6.40O1-755[»]
5LCWelectron microscopy4.00O1-755[»]
ProteinModelPortaliQ9UJX4
SMRiQ9UJX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati209 – 249TPR 1Add BLAST41
Repeati250 – 300TPR 2Add BLAST51
Repeati301 – 337TPR 3Add BLAST37
Repeati338 – 378TPR 4Add BLAST41
Repeati379 – 418TPR 5Add BLAST40
Repeati419 – 466TPR 6Add BLAST48
Repeati467 – 500TPR 7Add BLAST34
Repeati501 – 540TPR 8Add BLAST40
Repeati541 – 580TPR 9Add BLAST40
Repeati581 – 620TPR 10Add BLAST40
Repeati621 – 660TPR 11Add BLAST40
Repeati661 – 696TPR 12Add BLAST36
Repeati697 – 736TPR 13Add BLAST40

Domaini

The TPR repeats are six to seven residues longer than a canonical TPR motif.1 Publication

Sequence similaritiesi

Belongs to the APC5 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG4322 Eukaryota
ENOG410YVZN LUCA
GeneTreeiENSGT00390000018674
HOGENOMiHOG000033988
HOVERGENiHBG001285
InParanoidiQ9UJX4
KOiK03352
OMAiSCHTIAL
OrthoDBiEOG091G05K7
PhylomeDBiQ9UJX4
TreeFamiTF105444

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR037679 Apc5
IPR026000 Apc5_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR12830 PTHR12830, 1 hit
PfamiView protein in Pfam
PF12862 ANAPC5, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 4 hits
SUPFAMiSSF48452 SSF48452, 3 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJX4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVHESLYF NPMMTNGVVH ANVFGIKDWV TPYKIAVLVL LNEMSRTGEG
60 70 80 90 100
AVSLMERRRL NQLLLPLLQG PDITLSKLYK LIEESCPQLA NSVQIRIKLM
110 120 130 140 150
AEGELKDMEQ FFDDLSDSFS GTEPEVHKTS VVGLFLRHMI LAYSKLSFSQ
160 170 180 190 200
VFKLYTALQQ YFQNGEKKTV EDADMELTSR DEGERKMEKE ELDVSVREEE
210 220 230 240 250
VSCSGPLSQK QAEFFLSQQA SLLKNDETKA LTPASLQKEL NNLLKFNPDF
260 270 280 290 300
AEAHYLSYLN NLRVQDVFSS THSLLHYFDR LILTGAESKS NGEEGYGRSL
310 320 330 340 350
RYAALNLAAL HCRFGHYQQA ELALQEAIRI AQESNDHVCL QHCLSWLYVL
360 370 380 390 400
GQKRSDSYVL LEHSVKKAVH FGLPYLASLG IQSLVQQRAF AGKTANKLMD
410 420 430 440 450
ALKDSDLLHW KHSLSELIDI SIAQKTAIWR LYGRSTMALQ QAQMLLSMNS
460 470 480 490 500
LEAVNAGVQQ NNTESFAVAL CHLAELHAEQ GCFAAASEVL KHLKERFPPN
510 520 530 540 550
SQHAQLWMLC DQKIQFDRAM NDGKYHLADS LVTGITALNS IEGVYRKAVV
560 570 580 590 600
LQAQNQMSEA HKLLQKLLVH CQKLKNTEMV ISVLLSVAEL YWRSSSPTIA
610 620 630 640 650
LPMLLQALAL SKEYRLQYLA SETVLNLAFA QLILGIPEQA LSLLHMAIEP
660 670 680 690 700
ILADGAILDK GRAMFLVAKC QVASAASYDQ PKKAEALEAA IENLNEAKNY
710 720 730 740 750
FAKVDCKERI RDVVYFQARL YHTLGKTQER NRCAMLFRQL HQELPSHGVP

LINHL
Length:755
Mass (Da):85,077
Last modified:October 3, 2003 - v2
Checksum:i174F68EAB44760EB
GO
Isoform 2 (identifier: Q9UJX4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.
     122-131: TEPEVHKTSV → MLSPCLSSYF
     318-340: QQAELALQEAIRIAQESNDHVCL → TSPPWEYSPLFNRELLLGRRQTS
     341-755: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):25,147
Checksum:iE74D54AFF6C8744E
GO
Isoform 3 (identifier: Q9UJX4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.
     375-387: Missing.

Note: No experimental confirmation available.
Show »
Length:643
Mass (Da):72,526
Checksum:i6245611F5812F7CC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24F → L in AAF05753 (PubMed:9469815).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035793617Q → H in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0084661 – 121Missing in isoform 2. 1 PublicationAdd BLAST121
Alternative sequenceiVSP_0448781 – 99Missing in isoform 3. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_008467122 – 131TEPEVHKTSV → MLSPCLSSYF in isoform 2. 1 Publication10
Alternative sequenceiVSP_008468318 – 340QQAEL…DHVCL → TSPPWEYSPLFNRELLLGRR QTS in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_008469341 – 755Missing in isoform 2. 1 PublicationAdd BLAST415
Alternative sequenceiVSP_044879375 – 387Missing in isoform 3. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191339 mRNA Translation: AAF05753.1
AK025614 mRNA No translation available.
BX537687 mRNA Translation: CAD97812.1
AC048337 Genomic DNA No translation available.
AC069209 Genomic DNA No translation available.
BC001081 mRNA Translation: AAH01081.1
BC001950 mRNA Translation: AAH01950.1
BC006301 mRNA Translation: AAH06301.1
BC034243 mRNA Translation: AAH34243.1
CCDSiCCDS45000.1 [Q9UJX4-3]
CCDS9220.1 [Q9UJX4-1]
RefSeqiNP_001131031.1, NM_001137559.1 [Q9UJX4-3]
NP_001317418.1, NM_001330489.1
NP_057321.2, NM_016237.4 [Q9UJX4-1]
UniGeneiHs.7101

Genome annotation databases

EnsembliENST00000261819; ENSP00000261819; ENSG00000089053 [Q9UJX4-1]
ENST00000441917; ENSP00000415061; ENSG00000089053 [Q9UJX4-3]
GeneIDi51433
KEGGihsa:51433
UCSCiuc001uag.4 human [Q9UJX4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAPC5_HUMAN
AccessioniPrimary (citable) accession number: Q9UJX4
Secondary accession number(s): E9PFB2, Q8N4H7, Q9BQD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: June 20, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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