Reviewed, UniProtKB/Swiss-Prot Q9UJX3 (APC7_HUMAN)

Names and origin

Protein names

Recommended name:
    Anaphase-promoting complex subunit 7
      Short name=APC7
Alternative name(s):
    Cyclosome subunit 7

Gene names

Name:	ANAPC7
Synonyms:	APC7

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 11 subunits. Ref.1
Sequence similarities	Belongs to the APC7 family. Contains 7 TPR repeats.
Sequence caution	The sequence AAH17316.2 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Domain	Repeat TPR repeat
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 565

565

Anaphase-promoting complex subunit 7

PRO_0000106261

Regions

Repeat

43 – 76

34

TPR 1

Repeat

135 – 168

34

TPR 2

Repeat

237 – 270

34

TPR 3

Repeat

339 – 372

34

TPR 4

Repeat

407 – 441

35

TPR 5

Repeat

442 – 474

33

TPR 6

Repeat

475 – 508

34

TPR 7

Experimental info

Sequence conflict

150

1

Q → R in AAF05754. Ref.1

Sequence conflict

268

1

P → L in AAF05754. Ref.1

Sequence conflict

503

1

S → R in CAB70828. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

565

Helix Strand Turn

Details...

Helix

3 – 12

10

Helix

16 – 32

17

Helix

39 – 55

17

Helix

59 – 74

16

Helix

101 – 114

14

Helix

118 – 125

8

Helix

130 – 132

3

Helix

135 – 144

10

Sequences

	Sequence		Length	Mass (Da)
	Q9UJX3-1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: B86B7145E9E03EC3	FASTA	565	63,133
	10 20 30 40 50 60 MNVIDHVRDM AAAGLHSNVR LLSSLLLTMS NNNPELFSPP QKYQLLVYHA DSLFHDKEYR 70 80 90 100 110 120 NAVSKYTMAL QQKKALSKTS KVRPSTGNSA STPQSQCLPS EIEVKYKMAE CYTMLKQDKD 130 140 150 160 170 180 AIAILDGIPS RQRTPKINMM LANLYKKAGQ ERPSVTSYKE VLRQCPLALD AILGLLSLSV 190 200 210 220 230 240 KGAEVASMTM NVIQTVPNLD WLSVWIKAYA FVHTGDNSRA ISTICSLEKK SLLRDNVDLL 250 260 270 280 290 300 GSLADLYFRA GDNKNSVLKF EQAQMLDPYL IKGMDVYGYL LAREGRLEDV ENLGCRLFNI 310 320 330 340 350 360 SDQHAEPWVV SGCHSFYSKR YSRALYLGAK AIQLNSNSVQ ALLLKGAALR NMGRVQEAII 370 380 390 400 410 420 HFREAIRLAP CRLDCYEGLI ECYLASNSIR EAMVMANNVY KTLGANAQTL TLLATVCLED 430 440 450 460 470 480 PVTQEKAKTL LDKALTQRPD YIKAVVKKAE LLSREQKYED GIALLRNALA NQSDCVLHRI 490 500 510 520 530 540 LGDFLVAVNE YQEAMDQYSI ALSLDPNDQK SLEGMQKMEK EESPTDATQE EDVDDMEGSG 550 560 EEGDLEGSDS EAAQWADQEQ WFGMQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cullin homology region in a subunit of the anaphase-promoting complex." Yu H., Peters J.-M., King R.W., Page A.M., Hieter P., Kirschner M.W. Science 279:1219-1222(1998) [PubMed: 9469815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 110-565. Tissue: Ovary and Skin.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 282-503. Tissue: Testis.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C." Dube P., Herzog F., Gieffers C., Sander B., Riedel D., Mueller S.A., Engel A., Peters J.-M., Stark H. Mol. Cell 20:867-879(2005) [PubMed: 16364912] [Abstract] Cited for: ELECTRON MICROSCOPY OF THE APC/C.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF191340 mRNA. Translation: AAF05754.1.
BC002941 mRNA. Translation: AAH02941.2.
BC009498 mRNA. Translation: AAH09498.2.
BC017316 mRNA. Translation: AAH17316.2. Sequence problems.
AL137586 mRNA. Translation: CAB70828.1.

IPI

IPI00939488.

PIR

T46297.

UniGene

Hs.529280

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FFL	X-ray	2.50	A/B/C/D	1-147	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q9UJX3.

PTM databases

PhosphoSite

Q9UJX3.

Proteomic databases

PeptideAtlas

Q9UJX3.

PRIDE

Q9UJX3.

Genome annotation databases

Ensembl

ENST00000354390; ENSP00000346363; ENSG00000196510; Homo sapiens. [Genome view]
ENST00000455511; ENSP00000394394; ENSG00000196510; Homo sapiens. [Genome view]

NMPDR

fig|9606.3.peg.8242.

Organism-specific databases

GeneCards

GC12M109295.

HGNC

HGNC:17380. ANAPC7.

HPA

CAB009567.

MIM

606949. gene.

PharmGKB

PA134901290.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9UJX3.

HOVERGEN

Q9UJX3.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

ArrayExpress

Q9UJX3.

Bgee

Q9UJX3.

CleanEx

HS_ANAPC7.

Genevestigator

Q9UJX3.

GermOnline

ENSG00000196510. Homo sapiens.

Family and domain databases

InterPro

IPR001440. TPR-1.
IPR011990. TPR-like_helical.
IPR013026. TPR_region.
IPR019734. TPR_repeat.
[Graphical view]

Gene3D

G3DSA:1.25.40.10. TPR-like_helical. 2 hits.

Pfam

PF00515. TPR_1. 3 hits.
[Graphical view]

SMART

SM00028. TPR. 4 hits.
[Graphical view]

PROSITE

PS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

55005.

SOURCE

Search...

Entry information

Entry name

APC7_HUMAN

Accession

Primary (citable) accession number: Q9UJX3
Secondary accession number(s): Q96AC4 , Q96GF4, Q9BU24, Q9NT16

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	October 17, 2006
Last modified:	November 24, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.