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Q9UJW2 (TINAG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tubulointerstitial nephritis antigen

Short name=TIN-Ag
Gene names
Name:TINAG
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. This is a non catalytic peptidase C1 family protein. Ref.9

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane Ref.2 Ref.7.

Tissue specificity

Expressed in the kidney cortex, small intestine and cornea. Ref.1 Ref.2 Ref.7

Developmental stage

Initially observed in the Bowman capsule during early glomerular capillary loop formation in the kidney. In more developmentally mature glomeruli, following transition from early to mid-capillary loop stage, expression is higher in the proximal tubular basement membrane than in the distal basement membrane and Bowman capsule. Ref.10

Post-translational modification

It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus 2 alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Miscellaneous

Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.

Sequence similarities

Belongs to the peptidase C1 family.

Contains 1 SMB (somatomedin-B) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UJW2-1)

Also known as: TIN1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Major isoform.
Isoform 2 (identifier: Q9UJW2-2)

Also known as: TIN2;

The sequence of this isoform differs from the canonical sequence as follows:
     119-169: Missing.
     209-300: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Tubulointerstitial nephritis antigen
PRO_0000050600

Regions

Domain59 – 10749SMB

Sites

Site49 – 502Cleavage; by furin Potential Ref.2

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential
Disulfide bond63 ↔ 70Alternate By similarity
Disulfide bond70 ↔ 102Alternate By similarity
Disulfide bond81 ↔ 95Alternate By similarity
Disulfide bond81 ↔ 83Alternate By similarity
Disulfide bond87 ↔ 94 By similarity
Disulfide bond95 ↔ 102Alternate By similarity

Natural variations

Alternative sequence119 – 16951Missing in isoform 2. Ref.2
VSP_050567
Alternative sequence209 – 30092Missing in isoform 2. Ref.2
VSP_050568
Natural variant31T → A.
Corresponds to variant rs16885197 [ dbSNP | Ensembl ].
VAR_047091
Natural variant221Q → R.
Corresponds to variant rs2297980 [ dbSNP | Ensembl ].
VAR_047092
Natural variant1581S → P. Ref.1 Ref.2
Corresponds to variant rs1058768 [ dbSNP | Ensembl ].
VAR_047093
Natural variant4131V → I.
Corresponds to variant rs34011963 [ dbSNP | Ensembl ].
VAR_047094
Natural variant4331I → L.
Corresponds to variant rs3736352 [ dbSNP | Ensembl ].
VAR_047095

Experimental info

Sequence conflict7 – 82IL → FS in AAF08931. Ref.2
Sequence conflict7 – 82IL → FS in AAF08932. Ref.2
Sequence conflict1751N → I in AAF08932. Ref.2
Sequence conflict1991P → L in BAA84949. Ref.1
Sequence conflict3331H → D in AAF08931. Ref.2
Sequence conflict3331H → D in AAF08932. Ref.2
Sequence conflict3811R → H in BAA84949. Ref.1
Sequence conflict4211L → R in AAF08932. Ref.2
Sequence conflict4371S → F in AAF08931. Ref.2
Sequence conflict4371S → F in AAF08932. Ref.2
Sequence conflict4631I → V in AAF08931. Ref.2
Sequence conflict4631I → V in AAF08932. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (TIN1) [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: 6D90C734DAC3A5AE

FASTA47654,605
        10         20         30         40         50         60 
MWTGYKILIF SYLTTEIWME KQYLSQREVD LEAYFTRNHT VLQGTRFKRA IFQGQYCRNF 

        70         80         90        100        110        120 
GCCEDRDDGC VTEFYAANAL CYCDKFCDRE NSDCCPDYKS FCREEKEWPP HTQPWYPEGC 

       130        140        150        160        170        180 
FKDGQHYEEG SVIKENCNSC TCSGQQWKCS QHVCLVRSEL IEQVNKGDYG WTAQNYSQFW 

       190        200        210        220        230        240 
GMTLEDGFKF RLGTLPPSPM LLSMNEMTAS LPATTDLPEF FVASYKWPGW THGPLDQKNC 

       250        260        270        280        290        300 
AASWAFSTAS VAADRIAIQS KGRYTANLSP QNLISCCAKN RHGCNSGSID RAWWYLRKRG 

       310        320        330        340        350        360 
LVSHACYPLF KDQNATNNGC AMASRSDGRG KRHATKPCPN NVEKSNRIYQ CSPPYRVSSN 

       370        380        390        400        410        420 
ETEIMKEIMQ NGPVQAIMQV REDFFHYKTG IYRHVTSTNK ESEKYRKLQT HAVKLTGWGT 

       430        440        450        460        470 
LRGAQGQKEK FWIAANSWGK SWGENGYFRI LRGVNESDIE KLIIAAWGQL TSSDEP 

« Hide

Isoform 2 (TIN2) [UniParc].

Checksum: E708C6B55D9DBF9A
Show »

FASTA33338,724

References

« Hide 'large scale' references
[1]"Molecular cloning, expression, and chromosomal localization of a human tubulointerstitial nephritis antigen."
Ikeda M., Takemura T., Hino S., Yoshioka K.
Biochem. Biophys. Res. Commun. 268:225-230(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT PRO-158.
Tissue: Kidney.
[2]"Identification of two alternatively spliced forms of human tubulointerstitial nephritis antigen (TIN-Ag)."
Zhou B., Nelson T.R., Kashtan C., Gleason B., Michael A.F., Vlassi M., Charonis A.S.
J. Am. Soc. Nephrol. 11:658-668(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, VARIANT PRO-158.
Tissue: Kidney.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Identification of a target antigen in human anti-tubular basement membrane nephritis."
Fliger F.D., Wieslander J., Brentjens J.R., Andres G.A., Butkowski R.J.
Kidney Int. 31:800-807(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, INVOLVEMENT IN TUBULOINTERSTITIAL NEPHRITIS.
Tissue: Kidney.
[7]"Distribution of tubulointerstitial nephritis antigen and evidence for multiple forms."
Butkowski R.J., Kleppel M.M., Katz A., Michael A.F., Fish A.J.
Kidney Int. 40:838-846(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[8]"Role of antibodies to tubulointerstitial nephritis antigen in human anti-tubular basement membrane nephritis associated with membranous nephropathy."
Katz A., Fish A.J., Santamaria P., Nevins T.E., Kim Y., Butkowski R.J.
Am. J. Med. 93:691-698(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DISEASE.
[9]"Receptors in proximal tubular epithelial cells for tubulointerstitial nephritis antigen."
Chen Y., Krishnamurti U., Wayner E.A., Michael A.F., Charonis A.S.
Kidney Int. 49:153-157(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Tubulointerstitial nephritis antigen (TIN-ag) is expressed in distinct segments of the developing human nephron."
Nelson T.R., Kim Y., Michael A.F., Butkowski R.J., Charonis A.S.
Connect. Tissue Res. 37:53-60(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB022277 mRNA. Translation: BAA84949.1.
AF195116 mRNA. Translation: AAF08931.1.
AF195117 mRNA. Translation: AAF08932.1.
AK312918 mRNA. Translation: BAG35763.1.
AL589946, AL359380 Genomic DNA. Translation: CAH70302.1.
AL359380, AL589946 Genomic DNA. Translation: CAI16272.1.
CH471081 Genomic DNA. Translation: EAX04437.1.
PIRJC7189.
RefSeqNP_055279.3. NM_014464.3.
UniGeneHs.127011.

3D structure databases

ProteinModelPortalQ9UJW2.
SMRQ9UJW2. Positions 154-471.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118107. 2 interactions.
IntActQ9UJW2. 2 interactions.
STRING9606.ENSP00000259782.

Protein family/group databases

MEROPSC01.973.

PTM databases

PhosphoSiteQ9UJW2.

Polymorphism databases

DMDM317373501.

Proteomic databases

PaxDbQ9UJW2.
PRIDEQ9UJW2.

Protocols and materials databases

DNASU27283.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000259782; ENSP00000259782; ENSG00000137251. [Q9UJW2-1]
GeneID27283.
KEGGhsa:27283.
UCSCuc003pcj.2. human. [Q9UJW2-1]

Organism-specific databases

CTD27283.
GeneCardsGC06P054220.
H-InvDBHIX0025004.
HGNCHGNC:14599. TINAG.
HPAHPA035427.
MIM606749. gene.
neXtProtNX_Q9UJW2.
PharmGKBPA37905.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG310046.
HOGENOMHOG000241342.
HOVERGENHBG053961.
InParanoidQ9UJW2.
OMAWGQLTSS.
PhylomeDBQ9UJW2.
TreeFamTF313765.

Gene expression databases

ArrayExpressQ9UJW2.
BgeeQ9UJW2.
CleanExHS_TINAG.
GenevestigatorQ9UJW2.

Family and domain databases

InterProIPR025661. Pept_asp_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR001212. Somatomedin_B_dom.
[Graphical view]
PANTHERPTHR12411. PTHR12411. 1 hit.
PfamPF00112. Peptidase_C1. 1 hit.
PF01033. Somatomedin_B. 1 hit.
[Graphical view]
SMARTSM00645. Pept_C1. 1 hit.
SM00201. SO. 1 hit.
[Graphical view]
PROSITEPS00524. SMB_1. 1 hit.
PS50958. SMB_2. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTINAG.
GenomeRNAi27283.
NextBio50212.
PROQ9UJW2.
SOURCESearch...

Entry information

Entry nameTINAG_HUMAN
AccessionPrimary (citable) accession number: Q9UJW2
Secondary accession number(s): Q5T467, Q9UJW1, Q9ULZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 11, 2011
Last modified: April 16, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM