Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9UJW0 (DCTN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dynactin subunit 4

Short name=Dyn4
Alternative name(s):
Dynactin subunit p62
Gene names
Name:DCTN4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.

Subunit structure

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 By similarity. Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres By similarity. Ref.1 Ref.6

Subcellular location

Cytoplasmcytoskeleton. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Note: In skeletal muscles, demonstrates a punctate distribution along costameres By similarity. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels. Ref.1

Sequence similarities

Belongs to the dynactin subunit 4 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ANK2Q014841EBI-2134033,EBI-941975

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UJW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UJW0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
Isoform 3 (identifier: Q9UJW0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: S → SQHTIHVV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 460459Dynactin subunit 4
PRO_0000079823

Regions

Coiled coil152 – 17221 Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.7 Ref.9 Ref.10
Modified residue4071Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 5757Missing in isoform 2.
VSP_041306
Alternative sequence1791S → SQHTIHVV in isoform 3.
VSP_041307
Natural variant951P → T. Ref.5
Corresponds to variant rs11550931 [ dbSNP | Ensembl ].
VAR_054037
Natural variant2631Y → C.
Corresponds to variant rs35772018 [ dbSNP | Ensembl ].
VAR_033847
Natural variant3421F → L. Ref.2
Corresponds to variant rs11954652 [ dbSNP | Ensembl ].
VAR_024336
Natural variant4381S → N.
Corresponds to variant rs3733923 [ dbSNP | Ensembl ].
VAR_024337

Experimental info

Mutagenesis301C → S: Loss of ATP7B-binding; when associated with S-33; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis331C → S: Loss of ATP7B-binding; when associated with S-30; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis511C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis541C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis701C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis731C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-76; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis761C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-79; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis791C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-111; S-114; S-277 and S-280. Ref.6
Mutagenesis1111C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-114; S-277 and S-280. Ref.6
Mutagenesis1141C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-277 and S-280. Ref.6
Mutagenesis2771C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-280. Ref.6
Mutagenesis2801C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-277. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 2105D6C0A713B11D

FASTA46052,337
        10         20         30         40         50         60 
MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY CPSCLENMPS 

        70         80         90        100        110        120 
AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK TTMKKAYYLA CGFCRWTSRD 

       130        140        150        160        170        180 
VGMADKSVAS GGWQEPENPH TQRMNKLIEY YQQLAQKEKV ERDRKKLARR RNYMPLAFSD 

       190        200        210        220        230        240 
KYGLGTRLQR PRAGASISTL AGLSLKEGED QKEIKIEPAQ AVDEVEPLPE DYYTRPVNLT 

       250        260        270        280        290        300 
EVTTLQQRLL QPDFQPVCAS QLYPRHKHLL IKRSLRCRKC EHNLSKPEFN PTSIKFKIQL 

       310        320        330        340        350        360 
VAVNYIPEVR IMSIPNLRYM KESQVLLTLT NPVENLTHVT LFECEEGDPD DINSTAKVVV 

       370        380        390        400        410        420 
PPKELVLAGK DAAAEYDELA EPQDFQDDPD IIAFRKANKV GIFIKVTPQR EEGEVTVCFK 

       430        440        450        460 
MKHDFKNLAA PIRPIEESDQ GTEVIWLTQH VELSLGPLLP 

« Hide

Isoform 2 [UniParc].

Checksum: 4919CE2359032038
Show »

FASTA40345,757
Isoform 3 [UniParc].

Checksum: 2D4A445A6B764656
Show »

FASTA46753,152

References

« Hide 'large scale' references
[1]"A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1."
Karki S., Tokito M.K., Holzbaur E.L.F.
J. Biol. Chem. 275:4834-4839(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, SUBCELLULAR LOCATION.
Tissue: Neuron.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-342.
Tissue: Testis.
[3]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-95.
Tissue: Brain.
[6]"Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A."
Lim C.M., Cater M.A., Mercer J.F., La Fontaine S.
J. Biol. Chem. 281:14006-14014(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATP7B, MUTAGENESIS OF CYS-30; CYS-33; CYS-51; CYS-54; CYS-70; CYS-73; CYS-76; CYS-79; CYS-111; CYS-114; CYS-277 AND CYS-280.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF195120 mRNA. Translation: AAF03896.1.
AK125973 mRNA. Translation: BAG54272.1.
AK000299 mRNA. Translation: BAA91066.1.
AC008450 Genomic DNA. No translation available.
AC008453 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61706.1.
CH471062 Genomic DNA. Translation: EAW61707.1.
CH471062 Genomic DNA. Translation: EAW61709.1.
BC026323 mRNA. Translation: AAH26323.1.
CCDSCCDS4310.1. [Q9UJW0-1]
CCDS47310.1. [Q9UJW0-3]
CCDS47311.1. [Q9UJW0-2]
RefSeqNP_001129115.1. NM_001135643.1. [Q9UJW0-3]
NP_001129116.1. NM_001135644.1. [Q9UJW0-2]
NP_057305.1. NM_016221.3. [Q9UJW0-1]
UniGeneHs.675564.

3D structure databases

ProteinModelPortalQ9UJW0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119345. 15 interactions.
IntActQ9UJW0. 9 interactions.
MINTMINT-4535396.
STRING9606.ENSP00000414906.

PTM databases

PhosphoSiteQ9UJW0.

Polymorphism databases

DMDM62900106.

Proteomic databases

MaxQBQ9UJW0.
PaxDbQ9UJW0.
PeptideAtlasQ9UJW0.
PRIDEQ9UJW0.

Protocols and materials databases

DNASU51164.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000424236; ENSP00000411251; ENSG00000132912. [Q9UJW0-2]
ENST00000446090; ENSP00000414906; ENSG00000132912. [Q9UJW0-3]
ENST00000447998; ENSP00000416968; ENSG00000132912. [Q9UJW0-1]
GeneID51164.
KEGGhsa:51164.
UCSCuc003lsu.3. human. [Q9UJW0-1]
uc010jhi.3. human. [Q9UJW0-3]

Organism-specific databases

CTD51164.
GeneCardsGC05M150068.
HGNCHGNC:15518. DCTN4.
HPACAB017532.
MIM614758. gene.
neXtProtNX_Q9UJW0.
Orphanet586. Cystic fibrosis.
PharmGKBPA27183.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG147462.
HOGENOMHOG000007947.
HOVERGENHBG051324.
InParanoidQ9UJW0.
KOK10426.
OMAVGVFIKV.
OrthoDBEOG7SXW2X.
PhylomeDBQ9UJW0.
TreeFamTF105249.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9UJW0.
BgeeQ9UJW0.
CleanExHS_DCTN4.
GenevestigatorQ9UJW0.

Family and domain databases

InterProIPR008603. Dynactin_p62.
[Graphical view]
PANTHERPTHR13034. PTHR13034. 1 hit.
PfamPF05502. Dynactin_p62. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi51164.
NextBio54093.
PROQ9UJW0.
SOURCESearch...

Entry information

Entry nameDCTN4_HUMAN
AccessionPrimary (citable) accession number: Q9UJW0
Secondary accession number(s): B3KWW0 expand/collapse secondary AC list , D3DQH0, E5RGT5, Q8TAN8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM