Q9UJW0 (DCTN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dynactin subunit 4

Alternative name(s):
Dynactin subunit p62

Gene names

Name:

DCTN4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Subunit structure	Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 By similarity. Binds directly to the ACTR1A subunit of dynactin. Ref.1
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › centrosome. Note: Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels. Ref.1
Sequence similarities	Belongs to the dynactin subunit 4 family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	centrosome Inferred from direct assay. Source: UniProtKB nucleus Traceable author statement. Source: ProtInc
Molecular function	protein N-terminus binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANK2	Q01484	1	EBI-2134033,EBI-941975

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UJW0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UJW0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Isoform 3 (identifier: Q9UJW0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: S → SQHTIHVV

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 460	459	Dynactin subunit 4		PRO_0000079823
Regions
	Coiled coil	152 – 172	21	Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.6
Natural variations
	Alternative sequence	1 – 57	57	Missing in isoform 2.		VSP_041306
	Alternative sequence	179	1	S → SQHTIHVV in isoform 3.		VSP_041307
	Natural variant	95	1	P → T. Ref.5 Corresponds to variant rs11550931 [ dbSNP | Ensembl ].		VAR_054037
	Natural variant	263	1	Y → C. Corresponds to variant rs35772018 [ dbSNP | Ensembl ].		VAR_033847
	Natural variant	342	1	F → L. Ref.2 Corresponds to variant rs11954652 [ dbSNP | Ensembl ].		VAR_024336
	Natural variant	438	1	S → N. Corresponds to variant rs3733923 [ dbSNP | Ensembl ].		VAR_024337

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2105D6C0A713B11D	FASTA	460	52,337
	10 20 30 40 50 60 MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY CPSCLENMPS 70 80 90 100 110 120 AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK TTMKKAYYLA CGFCRWTSRD 130 140 150 160 170 180 VGMADKSVAS GGWQEPENPH TQRMNKLIEY YQQLAQKEKV ERDRKKLARR RNYMPLAFSD 190 200 210 220 230 240 KYGLGTRLQR PRAGASISTL AGLSLKEGED QKEIKIEPAQ AVDEVEPLPE DYYTRPVNLT 250 260 270 280 290 300 EVTTLQQRLL QPDFQPVCAS QLYPRHKHLL IKRSLRCRKC EHNLSKPEFN PTSIKFKIQL 310 320 330 340 350 360 VAVNYIPEVR IMSIPNLRYM KESQVLLTLT NPVENLTHVT LFECEEGDPD DINSTAKVVV 370 380 390 400 410 420 PPKELVLAGK DAAAEYDELA EPQDFQDDPD IIAFRKANKV GIFIKVTPQR EEGEVTVCFK 430 440 450 460 MKHDFKNLAA PIRPIEESDQ GTEVIWLTQH VELSLGPLLP
	Isoform 2 [UniParc]. Checksum: 4919CE2359032038	FASTA	403	45,757
	10 20 30 40 50 60 MPSAEAKLKK NRCANCFDCP GCMHTLSTRA TSISTQLPDD PAKTTMKKAY YLACGFCRWT 70 80 90 100 110 120 SRDVGMADKS VASGGWQEPE NPHTQRMNKL IEYYQQLAQK EKVERDRKKL ARRRNYMPLA 130 140 150 160 170 180 FSDKYGLGTR LQRPRAGASI STLAGLSLKE GEDQKEIKIE PAQAVDEVEP LPEDYYTRPV 190 200 210 220 230 240 NLTEVTTLQQ RLLQPDFQPV CASQLYPRHK HLLIKRSLRC RKCEHNLSKP EFNPTSIKFK 250 260 270 280 290 300 IQLVAVNYIP EVRIMSIPNL RYMKESQVLL TLTNPVENLT HVTLFECEEG DPDDINSTAK 310 320 330 340 350 360 VVVPPKELVL AGKDAAAEYD ELAEPQDFQD DPDIIAFRKA NKVGIFIKVT PQREEGEVTV 370 380 390 400 CFKMKHDFKN LAAPIRPIEE SDQGTEVIWL TQHVELSLGP LLP
	Isoform 3 [UniParc]. Checksum: 2D4A445A6B764656	FASTA	467	53,152
	10 20 30 40 50 60 MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY CPSCLENMPS 70 80 90 100 110 120 AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK TTMKKAYYLA CGFCRWTSRD 130 140 150 160 170 180 VGMADKSVAS GGWQEPENPH TQRMNKLIEY YQQLAQKEKV ERDRKKLARR RNYMPLAFSQ 190 200 210 220 230 240 HTIHVVDKYG LGTRLQRPRA GASISTLAGL SLKEGEDQKE IKIEPAQAVD EVEPLPEDYY 250 260 270 280 290 300 TRPVNLTEVT TLQQRLLQPD FQPVCASQLY PRHKHLLIKR SLRCRKCEHN LSKPEFNPTS 310 320 330 340 350 360 IKFKIQLVAV NYIPEVRIMS IPNLRYMKES QVLLTLTNPV ENLTHVTLFE CEEGDPDDIN 370 380 390 400 410 420 STAKVVVPPK ELVLAGKDAA AEYDELAEPQ DFQDDPDIIA FRKANKVGIF IKVTPQREEG 430 440 450 460 EVTVCFKMKH DFKNLAAPIR PIEESDQGTE VIWLTQHVEL SLGPLLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1." Karki S., Tokito M.K., Holzbaur E.L.F. J. Biol. Chem. 275:4834-4839(2000) [PubMed: 10671518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, SUBCELLULAR LOCATION. Tissue: Neuron.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-342. Tissue: Testis.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-95. Tissue: Brain.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 1), MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF195120 mRNA. Translation: AAF03896.1. AK125973 mRNA. Translation: BAG54272.1. AK000299 mRNA. Translation: BAA91066.1. AC008450 Genomic DNA. No translation available. AC008453 Genomic DNA. No translation available. CH471062 Genomic DNA. Translation: EAW61706.1. CH471062 Genomic DNA. Translation: EAW61707.1. CH471062 Genomic DNA. Translation: EAW61709.1. BC026323 mRNA. Translation: AAH26323.1.
IPI	IPI00550852. IPI00914016. IPI01010751.
RefSeq	NP_001129115.1. NM_001135643.1. NP_001129116.1. NM_001135644.1. NP_057305.1. NM_016221.3.
UniGene	Hs.675564.
3D structure databases
ProteinModelPortal	Q9UJW0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UJW0. 7 interactions.
STRING	Q9UJW0.
PTM databases
PhosphoSite	Q9UJW0.
Polymorphism databases
DMDM	62900106.
Proteomic databases
PeptideAtlas	Q9UJW0.
PRIDE	Q9UJW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000447998; ENSP00000416968; ENSG00000132912.
GeneID	51164.
KEGG	hsa:51164.
UCSC	uc003lsu.1. human.
Organism-specific databases
CTD	51164.
GeneCards	GC05M150068.
H-InvDB	HIX0005320.
HGNC	HGNC:15518. DCTN4.
HPA	CAB017532.
neXtProt	NX_Q9UJW0.
PharmGKB	PA27183.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19744.
GeneTree	ENSGT00390000006954.
HOVERGEN	HBG051324.
InParanoid	Q9UJW0.
OrthoDB	EOG4XKV6T.
PhylomeDB	Q9UJW0.
Gene expression databases
ArrayExpress	Q9UJW0.
Bgee	Q9UJW0.
CleanEx	HS_DCTN4.
Genevestigator	Q9UJW0.
GermOnline	ENSG00000132912. Homo sapiens.
Family and domain databases
InterPro	IPR008603. Dynactin_p62. [Graphical view]
KO	K10426.
PANTHER	PTHR13034. Dynactin_p62. 1 hit.
Pfam	PF05502. Dynactin_p62. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	54093.

Entry information

Entry name

DCTN4_HUMAN

Accession

Primary (citable) accession number: Q9UJW0
Secondary accession number(s): B3KWW0 , D3DQH0, E5RGT5, Q8TAN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.