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Protein

Dynactin subunit 4

Gene

DCTN4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132912-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 4
Short name:
Dyn4
Alternative name(s):
Dynactin subunit p62
Gene namesi
Name:DCTN4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:15518. DCTN4.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome 1 Publication

  • Note: In skeletal muscles, demonstrates a punctate distribution along costameres (By similarity). Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels.By similarity

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: ProtInc
  • cytoplasmic dynein complex Source: GO_Central
  • cytosol Source: Reactome
  • dynactin complex Source: GO_Central
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30C → S: Loss of ATP7B-binding; when associated with S-33; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi33C → S: Loss of ATP7B-binding; when associated with S-30; S-51; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi51C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-54; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi54C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-70, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi70C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-73; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi73C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-76; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi76C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-79; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi79C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-111; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi111C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-114; S-277 and S-280. 1 Publication1
Mutagenesisi114C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-277 and S-280. 1 Publication1
Mutagenesisi277C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-280. 1 Publication1
Mutagenesisi280C → S: Loss of ATP7B-binding; when associated with S-30; S-33; S-51; S-54, S-70; S-73; S-76; S-79; S-111; S-114 and S-277. 1 Publication1

Organism-specific databases

DisGeNETi51164.
MalaCardsiDCTN4.
OpenTargetsiENSG00000132912.
Orphaneti586. Cystic fibrosis.
PharmGKBiPA27183.

Polymorphism and mutation databases

BioMutaiDCTN4.
DMDMi62900106.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000798232 – 460Dynactin subunit 4Add BLAST459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei407PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UJW0.
PaxDbiQ9UJW0.
PeptideAtlasiQ9UJW0.
PRIDEiQ9UJW0.

PTM databases

iPTMnetiQ9UJW0.
PhosphoSitePlusiQ9UJW0.

Expressioni

Gene expression databases

BgeeiENSG00000132912.
CleanExiHS_DCTN4.
ExpressionAtlasiQ9UJW0. baseline and differential.
GenevisibleiQ9UJW0. HS.

Organism-specific databases

HPAiCAB017532.
HPA038026.

Interactioni

Subunit structurei

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131373EBI-2134033,EBI-739580
GOLGA2Q083794EBI-2134033,EBI-618309
MTUS2Q5JR594EBI-2134033,EBI-742948
PNMA1Q8ND903EBI-2134033,EBI-302345

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi119345. 43 interactors.
IntActiQ9UJW0. 19 interactors.
MINTiMINT-4535396.
STRINGi9606.ENSP00000414906.

Structurei

3D structure databases

ProteinModelPortaliQ9UJW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili152 – 172Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the dynactin subunit 4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3896. Eukaryota.
ENOG410ZCK8. LUCA.
GeneTreeiENSGT00390000006954.
HOGENOMiHOG000007947.
HOVERGENiHBG051324.
InParanoidiQ9UJW0.
KOiK10426.
OMAiFFMQYTY.
OrthoDBiEOG091G0FIJ.
PhylomeDBiQ9UJW0.
TreeFamiTF105249.

Family and domain databases

InterProiIPR008603. DCTN4.
[Graphical view]
PANTHERiPTHR13034. PTHR13034. 1 hit.
PfamiPF05502. Dynactin_p62. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLQSDRV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY
60 70 80 90 100
CPSCLENMPS AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK
110 120 130 140 150
TTMKKAYYLA CGFCRWTSRD VGMADKSVAS GGWQEPENPH TQRMNKLIEY
160 170 180 190 200
YQQLAQKEKV ERDRKKLARR RNYMPLAFSD KYGLGTRLQR PRAGASISTL
210 220 230 240 250
AGLSLKEGED QKEIKIEPAQ AVDEVEPLPE DYYTRPVNLT EVTTLQQRLL
260 270 280 290 300
QPDFQPVCAS QLYPRHKHLL IKRSLRCRKC EHNLSKPEFN PTSIKFKIQL
310 320 330 340 350
VAVNYIPEVR IMSIPNLRYM KESQVLLTLT NPVENLTHVT LFECEEGDPD
360 370 380 390 400
DINSTAKVVV PPKELVLAGK DAAAEYDELA EPQDFQDDPD IIAFRKANKV
410 420 430 440 450
GIFIKVTPQR EEGEVTVCFK MKHDFKNLAA PIRPIEESDQ GTEVIWLTQH
460
VELSLGPLLP
Length:460
Mass (Da):52,337
Last modified:May 1, 2000 - v1
Checksum:i2105D6C0A713B11D
GO
Isoform 2 (identifier: Q9UJW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Show »
Length:403
Mass (Da):45,757
Checksum:i4919CE2359032038
GO
Isoform 3 (identifier: Q9UJW0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-179: S → SQHTIHVV

Show »
Length:467
Mass (Da):53,152
Checksum:i2D4A445A6B764656
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05403795P → T.1 PublicationCorresponds to variant rs11550931dbSNPEnsembl.1
Natural variantiVAR_033847263Y → C.Corresponds to variant rs35772018dbSNPEnsembl.1
Natural variantiVAR_024336342F → L.1 PublicationCorresponds to variant rs11954652dbSNPEnsembl.1
Natural variantiVAR_024337438S → N.Corresponds to variant rs3733923dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0413061 – 57Missing in isoform 2. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_041307179S → SQHTIHVV in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195120 mRNA. Translation: AAF03896.1.
AK125973 mRNA. Translation: BAG54272.1.
AK000299 mRNA. Translation: BAA91066.1.
AC008450 Genomic DNA. No translation available.
AC008453 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61706.1.
CH471062 Genomic DNA. Translation: EAW61707.1.
CH471062 Genomic DNA. Translation: EAW61709.1.
BC026323 mRNA. Translation: AAH26323.1.
CCDSiCCDS4310.1. [Q9UJW0-1]
CCDS47310.1. [Q9UJW0-3]
CCDS47311.1. [Q9UJW0-2]
RefSeqiNP_001129115.1. NM_001135643.1. [Q9UJW0-3]
NP_001129116.1. NM_001135644.1. [Q9UJW0-2]
NP_057305.1. NM_016221.3. [Q9UJW0-1]
XP_011535946.1. XM_011537644.1. [Q9UJW0-2]
XP_011535947.1. XM_011537645.1. [Q9UJW0-2]
UniGeneiHs.675564.

Genome annotation databases

EnsembliENST00000424236; ENSP00000411251; ENSG00000132912. [Q9UJW0-2]
ENST00000446090; ENSP00000414906; ENSG00000132912. [Q9UJW0-3]
ENST00000447998; ENSP00000416968; ENSG00000132912. [Q9UJW0-1]
GeneIDi51164.
KEGGihsa:51164.
UCSCiuc003lsu.4. human. [Q9UJW0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195120 mRNA. Translation: AAF03896.1.
AK125973 mRNA. Translation: BAG54272.1.
AK000299 mRNA. Translation: BAA91066.1.
AC008450 Genomic DNA. No translation available.
AC008453 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61706.1.
CH471062 Genomic DNA. Translation: EAW61707.1.
CH471062 Genomic DNA. Translation: EAW61709.1.
BC026323 mRNA. Translation: AAH26323.1.
CCDSiCCDS4310.1. [Q9UJW0-1]
CCDS47310.1. [Q9UJW0-3]
CCDS47311.1. [Q9UJW0-2]
RefSeqiNP_001129115.1. NM_001135643.1. [Q9UJW0-3]
NP_001129116.1. NM_001135644.1. [Q9UJW0-2]
NP_057305.1. NM_016221.3. [Q9UJW0-1]
XP_011535946.1. XM_011537644.1. [Q9UJW0-2]
XP_011535947.1. XM_011537645.1. [Q9UJW0-2]
UniGeneiHs.675564.

3D structure databases

ProteinModelPortaliQ9UJW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119345. 43 interactors.
IntActiQ9UJW0. 19 interactors.
MINTiMINT-4535396.
STRINGi9606.ENSP00000414906.

PTM databases

iPTMnetiQ9UJW0.
PhosphoSitePlusiQ9UJW0.

Polymorphism and mutation databases

BioMutaiDCTN4.
DMDMi62900106.

Proteomic databases

EPDiQ9UJW0.
PaxDbiQ9UJW0.
PeptideAtlasiQ9UJW0.
PRIDEiQ9UJW0.

Protocols and materials databases

DNASUi51164.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000424236; ENSP00000411251; ENSG00000132912. [Q9UJW0-2]
ENST00000446090; ENSP00000414906; ENSG00000132912. [Q9UJW0-3]
ENST00000447998; ENSP00000416968; ENSG00000132912. [Q9UJW0-1]
GeneIDi51164.
KEGGihsa:51164.
UCSCiuc003lsu.4. human. [Q9UJW0-1]

Organism-specific databases

CTDi51164.
DisGeNETi51164.
GeneCardsiDCTN4.
HGNCiHGNC:15518. DCTN4.
HPAiCAB017532.
HPA038026.
MalaCardsiDCTN4.
MIMi614758. gene.
neXtProtiNX_Q9UJW0.
OpenTargetsiENSG00000132912.
Orphaneti586. Cystic fibrosis.
PharmGKBiPA27183.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3896. Eukaryota.
ENOG410ZCK8. LUCA.
GeneTreeiENSGT00390000006954.
HOGENOMiHOG000007947.
HOVERGENiHBG051324.
InParanoidiQ9UJW0.
KOiK10426.
OMAiFFMQYTY.
OrthoDBiEOG091G0FIJ.
PhylomeDBiQ9UJW0.
TreeFamiTF105249.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132912-MONOMER.
ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiDCTN4. human.
GenomeRNAii51164.
PROiQ9UJW0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132912.
CleanExiHS_DCTN4.
ExpressionAtlasiQ9UJW0. baseline and differential.
GenevisibleiQ9UJW0. HS.

Family and domain databases

InterProiIPR008603. DCTN4.
[Graphical view]
PANTHERiPTHR13034. PTHR13034. 1 hit.
PfamiPF05502. Dynactin_p62. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCTN4_HUMAN
AccessioniPrimary (citable) accession number: Q9UJW0
Secondary accession number(s): B3KWW0
, D3DQH0, E5RGT5, Q8TAN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.