Q9UJP4 (KLH21_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kelch-like protein 21

Gene names

Name:	KLHL21
Synonyms:	KIAA0469

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. Ref.8 Ref.9
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1. Ref.9
Subcellular location	Cytoplasm › cytoskeleton › spindle. Note: Localizes to the spindle midzone and targets CUL3 to this region. Ref.9
Sequence similarities	Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 6 Kelch repeats.
Sequence caution	The sequence BAA32314.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Kelch repeat Repeat
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	anaphase Inferred from mutant phenotype Ref.9. Source: UniProtKB cytokinesis Inferred from mutant phenotype Ref.9. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from direct assay Ref.9. Source: UniProtKB
Cellular_component	Cul3-RING ubiquitin ligase complex Inferred from direct assay Ref.9. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW polar microtubule Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UJP4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UJP4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     502-539: HVGGSLAVLG...VEAYDPETRA → NFQAGQHWKH...TAMMGGSHLN
     540-597: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Kelch-like protein 21		PRO_0000119125
Regions
	Domain	35 – 103	69	BTB
	Domain	138 – 239	102	BACK
	Repeat	287 – 335	49	Kelch 1
	Repeat	336 – 382	47	Kelch 2
	Repeat	384 – 422	39	Kelch 3
	Repeat	423 – 470	48	Kelch 4
	Repeat	472 – 512	41	Kelch 5
	Repeat	513 – 560	48	Kelch 6
Natural variations
	Alternative sequence	502 – 539	38	HVGGS…PETRA → NFQAGQHWKHRLVLILQPKC HRDECLGSTAMMGGSHLN in isoform 2.		VSP_032563
	Alternative sequence	540 – 597	58	Missing in isoform 2.		VSP_032564
Experimental info
	Mutagenesis	114 – 117	4	DLLQ → AALA: Abolishes interaction with CUL3. Ref.9
	Sequence conflict	183	1	R → C in BAC03453. Ref.6
	Sequence conflict	380	1	L → P in BAG52104. Ref.2
Isoform 2:
	Sequence conflict	534	1	G → D in BAA32314. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 4. Checksum: D3E682609F284645	FASTA	597	66,617
	10 20 30 40 50 60 MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY 70 80 90 100 110 120 FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA 130 140 150 160 170 180 VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR 190 200 210 220 230 240 LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAAHWPQL LEAVRLPFVR RFYLLAHVEA 250 260 270 280 290 300 EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE 310 320 330 340 350 360 LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN 370 380 390 400 410 420 EWAEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHTTD SWEALQPMTY PMDNCSTTAC 430 440 450 460 470 480 RGRLYAIGSL AGKETMVMQC YDPDTDLWSL VDCGQLPPWS FAPKTATLNG LMYFVRDDSA 490 500 510 520 530 540 EVDVYNPTRN EWDKIPSMNQ VHVGGSLAVL GGKLYVSGGY DNTFELSDVV EAYDPETRAW 550 560 570 580 590 SVVGRLPEPT FWHGSVSIFR QFMPQTFSGG RGFELDSGSD DMDPGRPRPP RDPDELH
	Isoform 2 [UniParc]. Checksum: DDE88D184C05A945	FASTA	539	60,378
	10 20 30 40 50 60 MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY 70 80 90 100 110 120 FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA 130 140 150 160 170 180 VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR 190 200 210 220 230 240 LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAAHWPQL LEAVRLPFVR RFYLLAHVEA 250 260 270 280 290 300 EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE 310 320 330 340 350 360 LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN 370 380 390 400 410 420 EWAEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHTTD SWEALQPMTY PMDNCSTTAC 430 440 450 460 470 480 RGRLYAIGSL AGKETMVMQC YDPDTDLWSL VDCGQLPPWS FAPKTATLNG LMYFVRDDSA 490 500 510 520 530 EVDVYNPTRN EWDKIPSMNQ VNFQAGQHWK HRLVLILQPK CHRDECLGST AMMGGSHLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hippocampus and Lung carcinoma.
[6]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-597 (ISOFORM 1). Tissue: Spleen.
[7]	Bienvenut W.V., Dhillon A.S., Kolch W. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 52-62; 99-122 AND 424-433 (ISOFORMS 1/2), PROTEIN SEQUENCE OF 561-571 (ISOFORM 1), MASS SPECTROMETRY. Tissue: Hepatoma.
[8]	"Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases." Furukawa M., He Y.J., Borchers C., Xiong Y. Nat. Cell Biol. 5:1001-1007(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
[9]	"The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis." Maerki S., Olma M.H., Staubli T., Steigemann P., Gerlich D.W., Quadroni M., Sumara I., Peter M. J. Cell Biol. 187:791-800(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, MUTAGENESIS OF 114-ASP--GLN-117.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007938 mRNA. Translation: BAA32314.2. Different initiation. AK075305 mRNA. Translation: BAG52104.1. AL591866 Genomic DNA. Translation: CAI16080.1. AL591866 Genomic DNA. Translation: CAI16082.1. CH471130 Genomic DNA. Translation: EAW71559.1. BC034039 mRNA. Translation: AAH34039.3. BC091648 mRNA. Translation: AAH91648.1. AK090472 mRNA. Translation: BAC03453.1.
IPI	IPI00006994. IPI00402021.
RefSeq	NP_055666.2. NM_014851.2.
UniGene	Hs.7764.
3D structure databases
ProteinModelPortal	Q9UJP4.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000366886.
PTM databases
PhosphoSite	Q9UJP4.
Polymorphism databases
DMDM	172044863.
Proteomic databases
PaxDb	Q9UJP4.
PRIDE	Q9UJP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000377658; ENSP00000366886; ENSG00000162413. ENST00000377663; ENSP00000366891; ENSG00000162413.
GeneID	9903.
KEGG	hsa:9903.
UCSC	uc001anz.1. human. uc001aoa.3. human.
Organism-specific databases
CTD	9903.
GeneCards	GC01M006586.
HGNC	HGNC:29041. KLHL21.
HPA	HPA051364.
neXtProt	NX_Q9UJP4.
PharmGKB	PA134989246.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG264826.
HOGENOM	HOG000261671.
HOVERGEN	HBG101644.
InParanoid	Q9UJP4.
KO	K10458.
OMA	WVRADPP.
OrthoDB	EOG45HRX2.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9UJP4.
Bgee	Q9UJP4.
CleanEx	HS_KLHL21.
Genevestigator	Q9UJP4.
GermOnline	ENSG00000162413. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.80. 1 hit. 3.30.710.10. 1 hit.
InterPro	IPR011705. BACK. IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR015916. Gal_Oxidase_b-propeller. IPR017096. Kelch-like_gigaxonin. IPR006652. Kelch_1. [Graphical view]
Pfam	PF07707. BACK. 1 hit. PF00651. BTB. 1 hit. PF01344. Kelch_1. 3 hits. [Graphical view]
PIRSF	PIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMART	SM00875. BACK. 1 hit. SM00225. BTB. 1 hit. SM00612. Kelch. 5 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	KLHL21. human.
GenomeRNAi	9903.
NextBio	37341.

Entry information

Entry name

KLH21_HUMAN

Accession

Primary (citable) accession number: Q9UJP4
Secondary accession number(s): B3KQP2 , O75057, Q5SY26, Q5SY28, Q8N4I6, Q8NF10

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 102 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.