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Protein

Kelch-like protein 21

Gene

KLHL21

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.2 Publications

Pathwayi

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. chromosome passenger complex localization to spindle midzone Source: UniProtKB
  3. mitotic nuclear division Source: UniProtKB-KW
  4. protein ubiquitination Source: UniProtKB
  5. regulation of cytokinesis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 21
Gene namesi
Name:KLHL21
Synonyms:KIAA0469
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:29041. KLHL21.

Subcellular locationi

Cytoplasmcytoskeletonspindle 1 Publication
Note: Localizes to the spindle midzone and targets CUL3 to this region.

GO - Cellular componenti

  1. centrosome Source: HPA
  2. Cul3-RING ubiquitin ligase complex Source: UniProtKB
  3. cytoplasm Source: UniProtKB-KW
  4. polar microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi114 – 1174DLLQ → AALA: Abolishes interaction with CUL3. 1 Publication

Organism-specific databases

PharmGKBiPA134989246.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Kelch-like protein 21PRO_0000119125Add
BLAST

Proteomic databases

MaxQBiQ9UJP4.
PaxDbiQ9UJP4.
PRIDEiQ9UJP4.

PTM databases

PhosphoSiteiQ9UJP4.

Expressioni

Gene expression databases

BgeeiQ9UJP4.
CleanExiHS_KLHL21.
ExpressionAtlasiQ9UJP4. baseline and differential.
GenevestigatoriQ9UJP4.

Organism-specific databases

HPAiHPA051364.
HPA059307.

Interactioni

Subunit structurei

Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1.1 Publication

Protein-protein interaction databases

BioGridi115232. 10 interactions.
STRINGi9606.ENSP00000366886.

Structurei

3D structure databases

ProteinModelPortaliQ9UJP4.
SMRiQ9UJP4. Positions 12-544.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 10369BTBPROSITE-ProRule annotationAdd
BLAST
Domaini138 – 239102BACKAdd
BLAST
Repeati287 – 33549Kelch 1Add
BLAST
Repeati336 – 38247Kelch 2Add
BLAST
Repeati384 – 42239Kelch 3Add
BLAST
Repeati423 – 47048Kelch 4Add
BLAST
Repeati472 – 51241Kelch 5Add
BLAST
Repeati513 – 56048Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG264826.
GeneTreeiENSGT00770000120450.
HOGENOMiHOG000261671.
HOVERGENiHBG101644.
InParanoidiQ9UJP4.
KOiK10458.
OMAiWVRADPP.
OrthoDBiEOG7WHH8X.
PhylomeDBiQ9UJP4.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR015916. Gal_Oxidase_b-propeller.
IPR017096. Kelch-like_gigaxonin-typ.
IPR006652. Kelch_1.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 3 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 5 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJP4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH
60 70 80 90 100
RAVLAAASPY FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA
110 120 130 140 150
VSGDNAEPLL RAADLLQFPA VKEACGAFLQ QQLDLANCLD MQDFAEAFSC
160 170 180 190 200
SGLASAAQRF ILRHVGELGA EQLERLPLAR LLRYLRDDGL CVPKEEAAYQ
210 220 230 240 250
LALRWVRADP PRRAAHWPQL LEAVRLPFVR RFYLLAHVEA EPLVARCPPC
260 270 280 290 300
LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE
310 320 330 340 350
LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD
360 370 380 390 400
CVWRYNSSVN EWAEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHTTD
410 420 430 440 450
SWEALQPMTY PMDNCSTTAC RGRLYAIGSL AGKETMVMQC YDPDTDLWSL
460 470 480 490 500
VDCGQLPPWS FAPKTATLNG LMYFVRDDSA EVDVYNPTRN EWDKIPSMNQ
510 520 530 540 550
VHVGGSLAVL GGKLYVSGGY DNTFELSDVV EAYDPETRAW SVVGRLPEPT
560 570 580 590
FWHGSVSIFR QFMPQTFSGG RGFELDSGSD DMDPGRPRPP RDPDELH
Length:597
Mass (Da):66,617
Last modified:March 18, 2008 - v4
Checksum:iD3E682609F284645
GO
Isoform 2 (identifier: Q9UJP4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     502-539: HVGGSLAVLG...VEAYDPETRA → NFQAGQHWKH...TAMMGGSHLN
     540-597: Missing.

Show »
Length:539
Mass (Da):60,378
Checksum:iDDE88D184C05A945
GO

Sequence cautioni

The sequence BAA32314.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831R → C in BAC03453 (Ref. 6) Curated
Sequence conflicti380 – 3801L → P in BAG52104 (PubMed:16303743).Curated
Isoform 2 (identifier: Q9UJP4-2)
Sequence conflicti534 – 5341G → D in BAA32314 (PubMed:9455484).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei502 – 53938HVGGS…PETRA → NFQAGQHWKHRLVLILQPKC HRDECLGSTAMMGGSHLN in isoform 2. 1 PublicationVSP_032563Add
BLAST
Alternative sequencei540 – 59758Missing in isoform 2. 1 PublicationVSP_032564Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007938 mRNA. Translation: BAA32314.2. Different initiation.
AK075305 mRNA. Translation: BAG52104.1.
AL591866 Genomic DNA. Translation: CAI16080.1.
AL591866 Genomic DNA. Translation: CAI16082.1.
CH471130 Genomic DNA. Translation: EAW71559.1.
BC034039 mRNA. Translation: AAH34039.3.
BC091648 mRNA. Translation: AAH91648.1.
AK090472 mRNA. Translation: BAC03453.1.
CCDSiCCDS30575.1. [Q9UJP4-1]
RefSeqiNP_055666.2. NM_014851.3. [Q9UJP4-1]
UniGeneiHs.7764.

Genome annotation databases

EnsembliENST00000377658; ENSP00000366886; ENSG00000162413. [Q9UJP4-1]
ENST00000377663; ENSP00000366891; ENSG00000162413. [Q9UJP4-2]
GeneIDi9903.
KEGGihsa:9903.
UCSCiuc001anz.1. human. [Q9UJP4-2]
uc001aoa.3. human. [Q9UJP4-1]

Polymorphism databases

DMDMi172044863.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007938 mRNA. Translation: BAA32314.2. Different initiation.
AK075305 mRNA. Translation: BAG52104.1.
AL591866 Genomic DNA. Translation: CAI16080.1.
AL591866 Genomic DNA. Translation: CAI16082.1.
CH471130 Genomic DNA. Translation: EAW71559.1.
BC034039 mRNA. Translation: AAH34039.3.
BC091648 mRNA. Translation: AAH91648.1.
AK090472 mRNA. Translation: BAC03453.1.
CCDSiCCDS30575.1. [Q9UJP4-1]
RefSeqiNP_055666.2. NM_014851.3. [Q9UJP4-1]
UniGeneiHs.7764.

3D structure databases

ProteinModelPortaliQ9UJP4.
SMRiQ9UJP4. Positions 12-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115232. 10 interactions.
STRINGi9606.ENSP00000366886.

PTM databases

PhosphoSiteiQ9UJP4.

Polymorphism databases

DMDMi172044863.

Proteomic databases

MaxQBiQ9UJP4.
PaxDbiQ9UJP4.
PRIDEiQ9UJP4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377658; ENSP00000366886; ENSG00000162413. [Q9UJP4-1]
ENST00000377663; ENSP00000366891; ENSG00000162413. [Q9UJP4-2]
GeneIDi9903.
KEGGihsa:9903.
UCSCiuc001anz.1. human. [Q9UJP4-2]
uc001aoa.3. human. [Q9UJP4-1]

Organism-specific databases

CTDi9903.
GeneCardsiGC01M006650.
HGNCiHGNC:29041. KLHL21.
HPAiHPA051364.
HPA059307.
neXtProtiNX_Q9UJP4.
PharmGKBiPA134989246.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG264826.
GeneTreeiENSGT00770000120450.
HOGENOMiHOG000261671.
HOVERGENiHBG101644.
InParanoidiQ9UJP4.
KOiK10458.
OMAiWVRADPP.
OrthoDBiEOG7WHH8X.
PhylomeDBiQ9UJP4.
TreeFamiTF329218.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiKLHL21. human.
GenomeRNAii9903.
NextBioi37341.
PROiQ9UJP4.

Gene expression databases

BgeeiQ9UJP4.
CleanExiHS_KLHL21.
ExpressionAtlasiQ9UJP4. baseline and differential.
GenevestigatoriQ9UJP4.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR015916. Gal_Oxidase_b-propeller.
IPR017096. Kelch-like_gigaxonin-typ.
IPR006652. Kelch_1.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 3 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 5 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
    Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O.
    DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hippocampus and Lung carcinoma.
  6. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 51-597 (ISOFORM 1).
    Tissue: Spleen.
  7. Bienvenut W.V., Dhillon A.S., Kolch W.
    Submitted (FEB-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 52-62; 99-122 AND 424-433 (ISOFORMS 1/2), PROTEIN SEQUENCE OF 561-571 (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hepatoma.
  8. "Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases."
    Furukawa M., He Y.J., Borchers C., Xiong Y.
    Nat. Cell Biol. 5:1001-1007(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
  9. "The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis."
    Maerki S., Olma M.H., Staubli T., Steigemann P., Gerlich D.W., Quadroni M., Sumara I., Peter M.
    J. Cell Biol. 187:791-800(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, MUTAGENESIS OF 114-ASP--GLN-117.

Entry informationi

Entry nameiKLH21_HUMAN
AccessioniPrimary (citable) accession number: Q9UJP4
Secondary accession number(s): B3KQP2
, O75057, Q5SY26, Q5SY28, Q8N4I6, Q8NF10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 18, 2008
Last modified: March 4, 2015
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.