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Q9UJF2

- NGAP_HUMAN

UniProt

Q9UJF2 - NGAP_HUMAN

Protein

Ras GTPase-activating protein nGAP

Gene

RASAL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (04 May 2001)
      Previous versions | rss
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    Functioni

    Inhibitory regulator of the Ras-cyclic AMP pathway.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Ras GTPase activator activity Source: RefGenome

    GO - Biological processi

    1. negative regulation of Ras protein signal transduction Source: RefGenome
    2. positive regulation of Ras GTPase activity Source: RefGenome
    3. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    GTPase activation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras GTPase-activating protein nGAP
    Alternative name(s):
    RAS protein activator-like 2
    Gene namesi
    Name:RASAL2
    Synonyms:NGAP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:9874. RASAL2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. intrinsic component of the cytoplasmic side of the plasma membrane Source: RefGenome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34235.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11391139Ras GTPase-activating protein nGAPPRO_0000056653Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei16 – 161Phosphoserine1 Publication
    Modified residuei89 – 891Phosphoserine1 Publication
    Modified residuei663 – 6631Phosphoserine1 Publication
    Modified residuei864 – 8641Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9UJF2.
    PaxDbiQ9UJF2.
    PRIDEiQ9UJF2.

    PTM databases

    PhosphoSiteiQ9UJF2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UJF2.
    BgeeiQ9UJF2.
    CleanExiHS_RASAL2.
    GenevestigatoriQ9UJF2.

    Organism-specific databases

    HPAiHPA018805.
    HPA020453.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q9WMX22EBI-359444,EBI-6863741From a different organism.

    Protein-protein interaction databases

    BioGridi114848. 8 interactions.
    DIPiDIP-27570N.
    IntActiQ9UJF2. 8 interactions.
    MINTiMINT-1137307.
    STRINGi9606.ENSP00000263528.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UJF2.
    SMRiQ9UJF2. Positions 285-625.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 158118PHAdd
    BLAST
    Domaini170 – 26697C2Add
    BLAST
    Domaini327 – 519193Ras-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi237 – 2437Poly-Lys
    Compositional biasi922 – 9254Poly-Ser

    Sequence similaritiesi

    Contains 1 C2 domain.Curated
    Contains 1 PH domain.Curated
    Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG245428.
    HOGENOMiHOG000231979.
    HOVERGENiHBG006492.
    KOiK17633.
    OMAiPSNSSME.
    OrthoDBiEOG74XS5P.
    PhylomeDBiQ9UJF2.
    TreeFamiTF105303.

    Family and domain databases

    Gene3Di1.10.506.10. 1 hit.
    2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR021887. DUF3498.
    IPR001849. PH_domain.
    IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF12004. DUF3498. 1 hit.
    PF00616. RasGAP. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00323. RasGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF49562. SSF49562. 1 hit.
    PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UJF2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQTPEVPAER SPRRRSISGT STSEKPNSMD TANTSPFKVP GFFSKRLKGS     50
    IKRTKSQSKL DRNTSFRLPS LRSTDDRSRG LPKLKESRSH ESLLSPCSTV 100
    ECLDLGRGEP VSVKPLHSSI LGQDFCFEVT YLSGSKCFSC NSASERDKWM 150
    ENLRRTVQPN KDNCRRAENV LRLWIIEAKD LAPKKKYFCE LCLDDTLFAR 200
    TTSKTKADNI FWGEHFEFFS LPPLHSITVH IYKDVEKKKK KDKNNYVGLV 250
    NIPTASVTGR QFVEKWYPVS TPTPNKGKTG GPSIRIKSRF QTITILPMEQ 300
    YKEFAEFVTS NYTMLCSVLE PVISVRNKEE LACALVHILQ STGRAKDFLT 350
    DLVMSEVDRC GEHDVLIFRE NTIATKSIEE YLKLVGQQYL HDALGEFIKA 400
    LYESDENCEV DPSKCSSSEL IDHQSNLKMC CELAFCKIIN SYCVFPRELK 450
    EVFASWKQQC LNRGKQDISE RLISASLFLR FLCPAIMSPS LFNLMQEYPD 500
    DRTSRTLTLI AKVIQNLANF AKFGNKEEYM AFMNDFLEHE WGGMKRFLLE 550
    ISNPDTISNT PGFDGYIDLG RELSVLHSLL WEVVSQLDKG ENSFLQATVA 600
    KLGPLPRVLA DITKSLTNPT PIQQQLRRFT EHNSSPNVSG SLSSGLQKIF 650
    EDPTDSDLHK LKSPSQDNTD SYFRGKTLLL VQQASSQSMT YSEKDERESS 700
    LPNGRSVSLM DLQDTHAAQV EHASVMLDVP IRLTGSQLSI TQVASIKQLR 750
    ETQSTPQSAP QVRRPLHPAL NQPGGLQPLS FQNPVYHLNN PIPAMPKASI 800
    DSSLENLSTA SSRSQSNSED FKLSGPSNSS MEDFTKRSTQ SEDFSRRHTV 850
    PDRHIPLALP RQNSTGQAQI RKVDQGGLGA RAKAPPSLPH SASLRSTGSM 900
    SVVSAALVAE PVQNGSRSRQ QSSSSRESPV PKVRAIQRQQ TQQVQSPVDS 950
    ATMSPVERTA AWVLNNGQYE EDVEETEQNL DEAKHAEKYE QEITKLKERL 1000
    RVSSRRLEEY ERRLLVQEQQ MQKLLLEYKA RLEDSEERLR RQQEEKDSQM 1050
    KSIISRLMAV EEELKKDHAE MQAVIDAKQK IIDAQEKRIV SLDSANTRLM 1100
    SALTQVKERY SMQVRNGISP TNPTKLSITE NGEFKNSSC 1139
    Length:1,139
    Mass (Da):128,558
    Last modified:May 4, 2001 - v2
    Checksum:i4A65C8243E1259A3
    GO
    Isoform 2 (identifier: Q9UJF2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-4: MQTP → MELSPSSGGA...EYPPEGATKL
         590-596: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,280
    Mass (Da):143,899
    Checksum:iD80E7D066C699560
    GO

    Sequence cautioni

    The sequence AAI10612.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti165 – 1651R → W in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035541
    Natural varianti379 – 3791E → D in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035542

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 44MQTP → MELSPSSGGAAEALSWPEMF PALESDSPLPPEDLDAVVPV SGAVAGGMLDRILLESVCQQ QSWVRVYDVKGPPTHRLSCG QSPYTETTTWERKYCILTDS QLVLLNKEKEIPVEGGQEQQ TDSTKGRCLRRTVSVPSEGQ FPEYPPEGATKL in isoform 2. 1 PublicationVSP_045777
    Alternative sequencei590 – 5967Missing in isoform 2. 1 PublicationVSP_045778

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047711 mRNA. Translation: AAD04814.1.
    AL035702, AL365357 Genomic DNA. Translation: CAI21912.1.
    AL365357, AL035702 Genomic DNA. Translation: CAI22693.1.
    AL160281 Genomic DNA. No translation available.
    AL499617 Genomic DNA. No translation available.
    BC110611 mRNA. Translation: AAI10612.1. Different initiation.
    CCDSiCCDS1321.2. [Q9UJF2-2]
    CCDS1322.1. [Q9UJF2-1]
    RefSeqiNP_004832.1. NM_004841.3. [Q9UJF2-1]
    NP_733793.2. NM_170692.2. [Q9UJF2-2]
    UniGeneiHs.496139.

    Genome annotation databases

    EnsembliENST00000367649; ENSP00000356621; ENSG00000075391. [Q9UJF2-2]
    ENST00000462775; ENSP00000420558; ENSG00000075391. [Q9UJF2-1]
    GeneIDi9462.
    KEGGihsa:9462.
    UCSCiuc001glr.3. human. [Q9UJF2-1]

    Polymorphism databases

    DMDMi13959419.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF047711 mRNA. Translation: AAD04814.1 .
    AL035702 , AL365357 Genomic DNA. Translation: CAI21912.1 .
    AL365357 , AL035702 Genomic DNA. Translation: CAI22693.1 .
    AL160281 Genomic DNA. No translation available.
    AL499617 Genomic DNA. No translation available.
    BC110611 mRNA. Translation: AAI10612.1 . Different initiation.
    CCDSi CCDS1321.2. [Q9UJF2-2 ]
    CCDS1322.1. [Q9UJF2-1 ]
    RefSeqi NP_004832.1. NM_004841.3. [Q9UJF2-1 ]
    NP_733793.2. NM_170692.2. [Q9UJF2-2 ]
    UniGenei Hs.496139.

    3D structure databases

    ProteinModelPortali Q9UJF2.
    SMRi Q9UJF2. Positions 285-625.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114848. 8 interactions.
    DIPi DIP-27570N.
    IntActi Q9UJF2. 8 interactions.
    MINTi MINT-1137307.
    STRINGi 9606.ENSP00000263528.

    PTM databases

    PhosphoSitei Q9UJF2.

    Polymorphism databases

    DMDMi 13959419.

    Proteomic databases

    MaxQBi Q9UJF2.
    PaxDbi Q9UJF2.
    PRIDEi Q9UJF2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000367649 ; ENSP00000356621 ; ENSG00000075391 . [Q9UJF2-2 ]
    ENST00000462775 ; ENSP00000420558 ; ENSG00000075391 . [Q9UJF2-1 ]
    GeneIDi 9462.
    KEGGi hsa:9462.
    UCSCi uc001glr.3. human. [Q9UJF2-1 ]

    Organism-specific databases

    CTDi 9462.
    GeneCardsi GC01P178063.
    HGNCi HGNC:9874. RASAL2.
    HPAi HPA018805.
    HPA020453.
    MIMi 606136. gene.
    neXtProti NX_Q9UJF2.
    PharmGKBi PA34235.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG245428.
    HOGENOMi HOG000231979.
    HOVERGENi HBG006492.
    KOi K17633.
    OMAi PSNSSME.
    OrthoDBi EOG74XS5P.
    PhylomeDBi Q9UJF2.
    TreeFami TF105303.

    Miscellaneous databases

    ChiTaRSi RASAL2. human.
    GeneWikii RASAL2.
    GenomeRNAii 9462.
    NextBioi 35446.
    PROi Q9UJF2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UJF2.
    Bgeei Q9UJF2.
    CleanExi HS_RASAL2.
    Genevestigatori Q9UJF2.

    Family and domain databases

    Gene3Di 1.10.506.10. 1 hit.
    2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR021887. DUF3498.
    IPR001849. PH_domain.
    IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF12004. DUF3498. 1 hit.
    PF00616. RasGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00233. PH. 1 hit.
    SM00323. RasGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF49562. SSF49562. 1 hit.
    PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel human RasGAP-like gene that maps within the prostate cancer susceptibility locus at chromosome 1q25."
      Noto S., Maeda T., Hattori S., Inazawa J., Imamura M., Asaka M., Hatakeyama M.
      FEBS Lett. 441:127-131(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Heart.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1139 (ISOFORM 2).
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-89 AND SER-663, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-165 AND ASP-379.

    Entry informationi

    Entry nameiNGAP_HUMAN
    AccessioniPrimary (citable) accession number: Q9UJF2
    Secondary accession number(s): F8W755
    , O95174, Q2TB22, Q5TFU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3