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Q9UJF2 (NGAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras GTPase-activating protein nGAP
Alternative name(s):
RAS protein activator-like 2
Gene names
Name:RASAL2
Synonyms:NGAP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1139 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitory regulator of the Ras-cyclic AMP pathway.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PH domain.

Contains 1 Ras-GAP domain.

Sequence caution

The sequence AAI10612.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q9WMX22EBI-359444,EBI-6863741From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UJF2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UJF2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MQTP → MELSPSSGGA...EYPPEGATKL
     590-596: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11391139Ras GTPase-activating protein nGAP
PRO_0000056653

Regions

Domain41 – 158118PH
Domain170 – 26697C2
Domain327 – 519193Ras-GAP
Compositional bias237 – 2437Poly-Lys
Compositional bias922 – 9254Poly-Ser

Amino acid modifications

Modified residue161Phosphoserine Ref.4
Modified residue891Phosphoserine Ref.4
Modified residue6631Phosphoserine Ref.4
Modified residue8641Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 44MQTP → MELSPSSGGAAEALSWPEMF PALESDSPLPPEDLDAVVPV SGAVAGGMLDRILLESVCQQ QSWVRVYDVKGPPTHRLSCG QSPYTETTTWERKYCILTDS QLVLLNKEKEIPVEGGQEQQ TDSTKGRCLRRTVSVPSEGQ FPEYPPEGATKL in isoform 2.
VSP_045777
Alternative sequence590 – 5967Missing in isoform 2.
VSP_045778
Natural variant1651R → W in a colorectal cancer sample; somatic mutation. Ref.9
VAR_035541
Natural variant3791E → D in a breast cancer sample; somatic mutation. Ref.9
VAR_035542

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 4, 2001. Version 2.
Checksum: 4A65C8243E1259A3

FASTA1,139128,558
        10         20         30         40         50         60 
MQTPEVPAER SPRRRSISGT STSEKPNSMD TANTSPFKVP GFFSKRLKGS IKRTKSQSKL 

        70         80         90        100        110        120 
DRNTSFRLPS LRSTDDRSRG LPKLKESRSH ESLLSPCSTV ECLDLGRGEP VSVKPLHSSI 

       130        140        150        160        170        180 
LGQDFCFEVT YLSGSKCFSC NSASERDKWM ENLRRTVQPN KDNCRRAENV LRLWIIEAKD 

       190        200        210        220        230        240 
LAPKKKYFCE LCLDDTLFAR TTSKTKADNI FWGEHFEFFS LPPLHSITVH IYKDVEKKKK 

       250        260        270        280        290        300 
KDKNNYVGLV NIPTASVTGR QFVEKWYPVS TPTPNKGKTG GPSIRIKSRF QTITILPMEQ 

       310        320        330        340        350        360 
YKEFAEFVTS NYTMLCSVLE PVISVRNKEE LACALVHILQ STGRAKDFLT DLVMSEVDRC 

       370        380        390        400        410        420 
GEHDVLIFRE NTIATKSIEE YLKLVGQQYL HDALGEFIKA LYESDENCEV DPSKCSSSEL 

       430        440        450        460        470        480 
IDHQSNLKMC CELAFCKIIN SYCVFPRELK EVFASWKQQC LNRGKQDISE RLISASLFLR 

       490        500        510        520        530        540 
FLCPAIMSPS LFNLMQEYPD DRTSRTLTLI AKVIQNLANF AKFGNKEEYM AFMNDFLEHE 

       550        560        570        580        590        600 
WGGMKRFLLE ISNPDTISNT PGFDGYIDLG RELSVLHSLL WEVVSQLDKG ENSFLQATVA 

       610        620        630        640        650        660 
KLGPLPRVLA DITKSLTNPT PIQQQLRRFT EHNSSPNVSG SLSSGLQKIF EDPTDSDLHK 

       670        680        690        700        710        720 
LKSPSQDNTD SYFRGKTLLL VQQASSQSMT YSEKDERESS LPNGRSVSLM DLQDTHAAQV 

       730        740        750        760        770        780 
EHASVMLDVP IRLTGSQLSI TQVASIKQLR ETQSTPQSAP QVRRPLHPAL NQPGGLQPLS 

       790        800        810        820        830        840 
FQNPVYHLNN PIPAMPKASI DSSLENLSTA SSRSQSNSED FKLSGPSNSS MEDFTKRSTQ 

       850        860        870        880        890        900 
SEDFSRRHTV PDRHIPLALP RQNSTGQAQI RKVDQGGLGA RAKAPPSLPH SASLRSTGSM 

       910        920        930        940        950        960 
SVVSAALVAE PVQNGSRSRQ QSSSSRESPV PKVRAIQRQQ TQQVQSPVDS ATMSPVERTA 

       970        980        990       1000       1010       1020 
AWVLNNGQYE EDVEETEQNL DEAKHAEKYE QEITKLKERL RVSSRRLEEY ERRLLVQEQQ 

      1030       1040       1050       1060       1070       1080 
MQKLLLEYKA RLEDSEERLR RQQEEKDSQM KSIISRLMAV EEELKKDHAE MQAVIDAKQK 

      1090       1100       1110       1120       1130 
IIDAQEKRIV SLDSANTRLM SALTQVKERY SMQVRNGISP TNPTKLSITE NGEFKNSSC 

« Hide

Isoform 2 [UniParc].

Checksum: D80E7D066C699560
Show »

FASTA1,280143,899

References

« Hide 'large scale' references
[1]"A novel human RasGAP-like gene that maps within the prostate cancer susceptibility locus at chromosome 1q25."
Noto S., Maeda T., Hattori S., Inazawa J., Imamura M., Asaka M., Hatakeyama M.
FEBS Lett. 441:127-131(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Heart.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1139 (ISOFORM 2).
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-89 AND SER-663, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-864, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-165 AND ASP-379.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF047711 mRNA. Translation: AAD04814.1.
AL035702, AL365357 Genomic DNA. Translation: CAI21912.1.
AL365357, AL035702 Genomic DNA. Translation: CAI22693.1.
AL160281 Genomic DNA. No translation available.
AL499617 Genomic DNA. No translation available.
BC110611 mRNA. Translation: AAI10612.1. Different initiation.
RefSeqNP_004832.1. NM_004841.3.
NP_733793.2. NM_170692.2.
UniGeneHs.496139.

3D structure databases

ProteinModelPortalQ9UJF2.
SMRQ9UJF2. Positions 109-165, 168-625.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114848. 8 interactions.
DIPDIP-27570N.
IntActQ9UJF2. 8 interactions.
MINTMINT-1137307.
STRING9606.ENSP00000263528.

PTM databases

PhosphoSiteQ9UJF2.

Polymorphism databases

DMDM13959419.

Proteomic databases

PaxDbQ9UJF2.
PRIDEQ9UJF2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367649; ENSP00000356621; ENSG00000075391. [Q9UJF2-2]
ENST00000462775; ENSP00000420558; ENSG00000075391. [Q9UJF2-1]
GeneID9462.
KEGGhsa:9462.
UCSCuc001glr.3. human. [Q9UJF2-1]

Organism-specific databases

CTD9462.
GeneCardsGC01P178063.
HGNCHGNC:9874. RASAL2.
HPAHPA018805.
HPA020453.
MIM606136. gene.
neXtProtNX_Q9UJF2.
PharmGKBPA34235.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG245428.
HOGENOMHOG000231979.
HOVERGENHBG006492.
KOK17633.
OMAPSNSSME.
OrthoDBEOG74XS5P.
PhylomeDBQ9UJF2.
TreeFamTF105303.

Gene expression databases

ArrayExpressQ9UJF2.
BgeeQ9UJF2.
CleanExHS_RASAL2.
GenevestigatorQ9UJF2.

Family and domain databases

Gene3D1.10.506.10. 1 hit.
2.60.40.150. 1 hit.
InterProIPR000008. C2_dom.
IPR021887. DUF3498.
IPR001849. Pleckstrin_homology.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamPF00168. C2. 1 hit.
PF12004. DUF3498. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
PROSITEPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRASAL2. human.
GeneWikiRASAL2.
GenomeRNAi9462.
NextBio35446.
PROQ9UJF2.
SOURCESearch...

Entry information

Entry nameNGAP_HUMAN
AccessionPrimary (citable) accession number: Q9UJF2
Secondary accession number(s): F8W755 expand/collapse secondary AC list , O95174, Q2TB22, Q5TFU9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM