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Protein

Ras GTPase-activating protein nGAP

Gene

RASAL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway.

Caution

GO - Molecular functioni

GO - Biological processi

  • MAPK cascade Source: Reactome
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-5658442 Regulation of RAS by GAPs
R-HSA-6802949 Signaling by RAS mutants

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein nGAP
Alternative name(s):
RAS protein activator-like 2
Gene namesi
Name:RASAL2
Synonyms:NGAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000075391.16
HGNCiHGNC:9874 RASAL2
MIMi606136 gene
neXtProtiNX_Q9UJF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi9462
OpenTargetsiENSG00000075391
PharmGKBiPA34235

Polymorphism and mutation databases

BioMutaiRASAL2
DMDMi13959419

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566531 – 1139Ras GTPase-activating protein nGAPAdd BLAST1139

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei620PhosphothreonineCombined sources1
Modified residuei663PhosphoserineCombined sources1
Modified residuei864PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UJF2
PaxDbiQ9UJF2
PeptideAtlasiQ9UJF2
PRIDEiQ9UJF2

PTM databases

iPTMnetiQ9UJF2
PhosphoSitePlusiQ9UJF2

Expressioni

Gene expression databases

BgeeiENSG00000075391
CleanExiHS_RASAL2
ExpressionAtlasiQ9UJF2 baseline and differential
GenevisibleiQ9UJF2 HS

Organism-specific databases

HPAiHPA018805
HPA020453

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi11484865 interactors.
CORUMiQ9UJF2
DIPiDIP-27570N
IntActiQ9UJF2 67 interactors.
MINTiQ9UJF2
STRINGi9606.ENSP00000356621

Structurei

3D structure databases

ProteinModelPortaliQ9UJF2
SMRiQ9UJF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 158PHAdd BLAST118
Domaini170 – 266C2Add BLAST97
Domaini327 – 519Ras-GAPPROSITE-ProRule annotationAdd BLAST193

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi237 – 243Poly-Lys7
Compositional biasi922 – 925Poly-Ser4

Phylogenomic databases

eggNOGiKOG3508 Eukaryota
ENOG410XPU1 LUCA
GeneTreeiENSGT00760000119092
HOGENOMiHOG000231979
HOVERGENiHBG006492
InParanoidiQ9UJF2
KOiK17633
OMAiQSNSEDF
OrthoDBiEOG091G00ZZ
PhylomeDBiQ9UJF2
TreeFamiTF105303

Family and domain databases

Gene3Di2.60.40.1501 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR021887 DUF3498
IPR001849 PH_domain
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF12004 DUF3498, 1 hit
PF00616 RasGAP, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00233 PH, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJF2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTPEVPAER SPRRRSISGT STSEKPNSMD TANTSPFKVP GFFSKRLKGS
60 70 80 90 100
IKRTKSQSKL DRNTSFRLPS LRSTDDRSRG LPKLKESRSH ESLLSPCSTV
110 120 130 140 150
ECLDLGRGEP VSVKPLHSSI LGQDFCFEVT YLSGSKCFSC NSASERDKWM
160 170 180 190 200
ENLRRTVQPN KDNCRRAENV LRLWIIEAKD LAPKKKYFCE LCLDDTLFAR
210 220 230 240 250
TTSKTKADNI FWGEHFEFFS LPPLHSITVH IYKDVEKKKK KDKNNYVGLV
260 270 280 290 300
NIPTASVTGR QFVEKWYPVS TPTPNKGKTG GPSIRIKSRF QTITILPMEQ
310 320 330 340 350
YKEFAEFVTS NYTMLCSVLE PVISVRNKEE LACALVHILQ STGRAKDFLT
360 370 380 390 400
DLVMSEVDRC GEHDVLIFRE NTIATKSIEE YLKLVGQQYL HDALGEFIKA
410 420 430 440 450
LYESDENCEV DPSKCSSSEL IDHQSNLKMC CELAFCKIIN SYCVFPRELK
460 470 480 490 500
EVFASWKQQC LNRGKQDISE RLISASLFLR FLCPAIMSPS LFNLMQEYPD
510 520 530 540 550
DRTSRTLTLI AKVIQNLANF AKFGNKEEYM AFMNDFLEHE WGGMKRFLLE
560 570 580 590 600
ISNPDTISNT PGFDGYIDLG RELSVLHSLL WEVVSQLDKG ENSFLQATVA
610 620 630 640 650
KLGPLPRVLA DITKSLTNPT PIQQQLRRFT EHNSSPNVSG SLSSGLQKIF
660 670 680 690 700
EDPTDSDLHK LKSPSQDNTD SYFRGKTLLL VQQASSQSMT YSEKDERESS
710 720 730 740 750
LPNGRSVSLM DLQDTHAAQV EHASVMLDVP IRLTGSQLSI TQVASIKQLR
760 770 780 790 800
ETQSTPQSAP QVRRPLHPAL NQPGGLQPLS FQNPVYHLNN PIPAMPKASI
810 820 830 840 850
DSSLENLSTA SSRSQSNSED FKLSGPSNSS MEDFTKRSTQ SEDFSRRHTV
860 870 880 890 900
PDRHIPLALP RQNSTGQAQI RKVDQGGLGA RAKAPPSLPH SASLRSTGSM
910 920 930 940 950
SVVSAALVAE PVQNGSRSRQ QSSSSRESPV PKVRAIQRQQ TQQVQSPVDS
960 970 980 990 1000
ATMSPVERTA AWVLNNGQYE EDVEETEQNL DEAKHAEKYE QEITKLKERL
1010 1020 1030 1040 1050
RVSSRRLEEY ERRLLVQEQQ MQKLLLEYKA RLEDSEERLR RQQEEKDSQM
1060 1070 1080 1090 1100
KSIISRLMAV EEELKKDHAE MQAVIDAKQK IIDAQEKRIV SLDSANTRLM
1110 1120 1130
SALTQVKERY SMQVRNGISP TNPTKLSITE NGEFKNSSC
Length:1,139
Mass (Da):128,558
Last modified:May 4, 2001 - v2
Checksum:i4A65C8243E1259A3
GO
Isoform 2 (identifier: Q9UJF2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MQTP → MELSPSSGGA...EYPPEGATKL
     590-596: Missing.

Note: No experimental confirmation available.
Show »
Length:1,280
Mass (Da):143,899
Checksum:iD80E7D066C699560
GO

Sequence cautioni

The sequence AAI10612 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035541165R → W in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs765646105Ensembl.1
Natural variantiVAR_035542379E → D in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0457771 – 4MQTP → MELSPSSGGAAEALSWPEMF PALESDSPLPPEDLDAVVPV SGAVAGGMLDRILLESVCQQ QSWVRVYDVKGPPTHRLSCG QSPYTETTTWERKYCILTDS QLVLLNKEKEIPVEGGQEQQ TDSTKGRCLRRTVSVPSEGQ FPEYPPEGATKL in isoform 2. 1 Publication4
Alternative sequenceiVSP_045778590 – 596Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047711 mRNA Translation: AAD04814.1
AL035702 Genomic DNA No translation available.
AL365357 Genomic DNA No translation available.
AL160281 Genomic DNA No translation available.
AL499617 Genomic DNA No translation available.
BC110611 mRNA Translation: AAI10612.1 Different initiation.
CCDSiCCDS1321.2 [Q9UJF2-2]
CCDS1322.1 [Q9UJF2-1]
RefSeqiNP_004832.1, NM_004841.3 [Q9UJF2-1]
NP_733793.2, NM_170692.2 [Q9UJF2-2]
UniGeneiHs.496139

Genome annotation databases

EnsembliENST00000367649; ENSP00000356621; ENSG00000075391 [Q9UJF2-2]
ENST00000462775; ENSP00000420558; ENSG00000075391 [Q9UJF2-1]
GeneIDi9462
KEGGihsa:9462
UCSCiuc001glq.4 human [Q9UJF2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNGAP_HUMAN
AccessioniPrimary (citable) accession number: Q9UJF2
Secondary accession number(s): F8W755
, O95174, Q2TB22, Q5TFU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: March 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome