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Protein

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6

Gene

TRMT6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508). Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29107537, PubMed:29072297).3 Publications

Caution

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei349SubstrateCombined sources1 Publication1
Binding sitei377SubstrateCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

  • mRNA methylation Source: UniProtKB
  • tRNA methylation Source: InterPro
  • tRNA modification Source: Reactome

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BRENDAi2.1.1.220 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Alternative name(s):
mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM61 Publication
tRNA(m1A58)-methyltransferase subunit TRM6
Short name:
tRNA(m1A58)MTase subunit TRM6
Gene namesi
Name:TRMT6
Synonyms:KIAA1153, TRM6
ORF Names:CGI-09
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000089195.14
HGNCiHGNC:20900 TRMT6
neXtProtiNX_Q9UJA5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi51605
OpenTargetsiENSG00000089195
PharmGKBiPA162407063

Polymorphism and mutation databases

DMDMi74753354

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002330981 – 497tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Add BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107PhosphothreonineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei305PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UJA5
PaxDbiQ9UJA5
PeptideAtlasiQ9UJA5
PRIDEiQ9UJA5

PTM databases

iPTMnetiQ9UJA5
PhosphoSitePlusiQ9UJA5

Expressioni

Tissue specificityi

Expressed in brain, liver, testis and ovary.1 Publication

Gene expression databases

BgeeiENSG00000089195
CleanExiHS_TRMT6
GenevisibleiQ9UJA5 HS

Organism-specific databases

HPAiHPA047032
HPA050408

Interactioni

Subunit structurei

Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.1 Publication1 Publication

Protein-protein interaction databases

BioGridi11963454 interactors.
IntActiQ9UJA5 14 interactors.
MINTiQ9UJA5
STRINGi9606.ENSP00000203001

Structurei

Secondary structure

1497
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 30Combined sources6
Beta strandi33 – 38Combined sources6
Beta strandi44 – 46Combined sources3
Beta strandi51 – 53Combined sources3
Helixi54 – 56Combined sources3
Beta strandi59 – 61Combined sources3
Beta strandi64 – 74Combined sources11
Helixi108 – 116Combined sources9
Helixi122 – 130Combined sources9
Beta strandi132 – 134Combined sources3
Helixi135 – 137Combined sources3
Helixi140 – 154Combined sources15
Beta strandi159 – 161Combined sources3
Helixi165 – 175Combined sources11
Helixi177 – 180Combined sources4
Helixi185 – 194Combined sources10
Beta strandi202 – 207Combined sources6
Helixi212 – 221Combined sources10
Beta strandi225 – 232Combined sources8
Helixi239 – 243Combined sources5
Helixi248 – 251Combined sources4
Beta strandi254 – 258Combined sources5
Helixi259 – 261Combined sources3
Helixi262 – 267Combined sources6
Helixi342 – 365Combined sources24
Beta strandi369 – 375Combined sources7
Helixi380 – 387Combined sources8
Helixi388 – 390Combined sources3
Beta strandi396 – 404Combined sources9
Helixi405 – 417Combined sources13
Beta strandi420 – 434Combined sources15
Beta strandi441 – 446Combined sources6
Beta strandi448 – 450Combined sources3
Beta strandi452 – 458Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CCBX-ray2.00B1-497[»]
5CCXX-ray2.10B1-497[»]
5CD1X-ray3.60B/E1-497[»]
ProteinModelPortaliQ9UJA5
SMRiQ9UJA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 104SubstrateCombined sources1 PublicationAdd BLAST11
Regioni145 – 154SubstrateCombined sources1 Publication10
Regioni175 – 182SubstrateCombined sources1 Publication8
Regioni415 – 423SubstrateCombined sources1 Publication9
Regioni434 – 441SubstrateCombined sources1 Publication8

Sequence similaritiesi

Belongs to the TRM6/GCD10 family.Curated

Phylogenomic databases

eggNOGiKOG1416 Eukaryota
ENOG410XSXD LUCA
GeneTreeiENSGT00390000008327
HOGENOMiHOG000232007
HOVERGENiHBG061231
InParanoidiQ9UJA5
KOiK03256
OMAiRKCPESD
OrthoDBiEOG091G095O
PhylomeDBiQ9UJA5
TreeFamiTF314835

Family and domain databases

InterProiView protein in InterPro
IPR017423 TRM6
PANTHERiPTHR12945 PTHR12945, 1 hit
PfamiView protein in Pfam
PF04189 Gcd10p, 1 hit
PIRSFiPIRSF038170 tRNA_m1A_mtfrase, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSGEQPGP QPQHPGDHRI RDGDFVVLKR EDVFKAVQVQ RRKKVTFEKQ
60 70 80 90 100
WFYLDNVIGH SYGTAFEVTS GGSLQPKKKR EEPTAETKEA GTDNRNIVDD
110 120 130 140 150
GKSQKLTQDD IKALKDKGIK GEEIVQQLIE NSTTFRDKTE FAQDKYIKKK
160 170 180 190 200
KKKYEAIITV VKPSTRILSI MYYAREPGKI NHMRYDTLAQ MLTLGNIRAG
210 220 230 240 250
NKMIVMETCA GLVLGAMMER MGGFGSIIQL YPGGGPVRAA TACFGFPKSF
260 270 280 290 300
LSGLYEFPLN KVDSLLHGTF SAKMLSSEPK DSALVEESNG TLEEKQASEQ
310 320 330 340 350
ENEDSMAEAP ESNHPEDQET METISQDPEH KGPKERGSKK DYIQEKQRRQ
360 370 380 390 400
EEQRKRHLEA AALLSERNAD GLIVASRFHP TPLLLSLLDF VAPSRPFVVY
410 420 430 440 450
CQYKEPLLEC YTKLRERGGV INLRLSETWL RNYQVLPDRS HPKLLMSGGG
460 470 480 490
GYLLSGFTVA MDNLKADTSL KSNASTLESH ETEEPAAKKR KCPESDS
Length:497
Mass (Da):55,799
Last modified:May 1, 2000 - v1
Checksum:i920800D0722A6CBB
GO
Isoform 2 (identifier: Q9UJA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-497: LIVASRFHPT...KKRKCPESDS → CVDGRRQETPQCMWALLQTDWHSLSPCL

Show »
Length:399
Mass (Da):44,963
Checksum:i28C0FB314555F56C
GO
Isoform 3 (identifier: Q9UJA5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     344-365: QEKQRRQEEQRKRHLEAAALLS → SGKNRGRQGRSSGKDFWGCRFA
     366-497: Missing.

Note: No experimental confirmation available.
Show »
Length:365
Mass (Da):40,816
Checksum:i15B0559642D8D087
GO
Isoform 4 (identifier: Q9UJA5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-170: Missing.

Show »
Length:327
Mass (Da):36,439
Checksum:iF01F65B739562EE6
GO

Sequence cautioni

The sequence BAA86467 differs from that shown. Reason: Frameshift at position 319.Curated
The sequence BAA86467 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053789293E → K. Corresponds to variant dbSNP:rs6139876Ensembl.1
Natural variantiVAR_053790299E → G2 PublicationsCorresponds to variant dbSNP:rs451571Ensembl.1
Natural variantiVAR_053791333P → L. Corresponds to variant dbSNP:rs35203742Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0547401 – 170Missing in isoform 4. 1 PublicationAdd BLAST170
Alternative sequenceiVSP_031100344 – 365QEKQR…AALLS → SGKNRGRQGRSSGKDFWGCR FA in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_031101366 – 497Missing in isoform 3. 1 PublicationAdd BLAST132
Alternative sequenceiVSP_018025372 – 497LIVAS…PESDS → CVDGRRQETPQCMWALLQTD WHSLSPCL in isoform 2. 1 PublicationAdd BLAST126

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032979 mRNA Translation: BAA86467.1 Different initiation.
AF132943 mRNA Translation: AAD27718.1 Frameshift.
AK000613 mRNA No translation available.
AK300812 mRNA Translation: BAG62468.1
AL035461 Genomic DNA No translation available.
BC001262 mRNA Translation: AAH01262.1
CCDSiCCDS13093.1 [Q9UJA5-1]
CCDS63225.1 [Q9UJA5-4]
RefSeqiNP_001268396.1, NM_001281467.1 [Q9UJA5-4]
NP_057023.2, NM_015939.4 [Q9UJA5-1]
UniGeneiHs.128791

Genome annotation databases

EnsembliENST00000203001; ENSP00000203001; ENSG00000089195 [Q9UJA5-1]
ENST00000453074; ENSP00000392070; ENSG00000089195 [Q9UJA5-4]
GeneIDi51605
KEGGihsa:51605
UCSCiuc002wmh.3 human [Q9UJA5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTRM6_HUMAN
AccessioniPrimary (citable) accession number: Q9UJA5
Secondary accession number(s): B4DUV6
, Q76P92, Q9BQV5, Q9ULR7, Q9Y2Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: February 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome