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Protein

N-acetyl-D-glucosamine kinase

Gene

NAGK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway: although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded. Also has ManNAc kinase activity.1 Publication

Catalytic activityi

ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate.1 Publication

Pathwayi: N-acetylneuraminate degradation

This protein is involved in the pathway N-acetylneuraminate degradation, which is part of Amino-sugar metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13ATP; via amide nitrogenCombined sources1 Publication1
Binding sitei36SubstrateCombined sources1 Publication1
Binding sitei107SubstrateCombined sources1 Publication1
Binding sitei127ATPCombined sources1 Publication1
Binding sitei152SubstrateCombined sources1 Publication1
Binding sitei214ATP; via carbonyl oxygenCombined sources1 Publication1
Binding sitei271ATPCombined sources1 Publication1
Binding sitei275ATPCombined sources1 Publication1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • N-acetylglucosamine kinase activity Source: UniProtKB

GO - Biological processi

  • N-acetylglucosamine metabolic process Source: ProtInc
  • N-acetylmannosamine metabolic process Source: ProtInc
  • N-acetylneuraminate catabolic process Source: UniProtKB
  • UDP-N-acetylglucosamine biosynthetic process Source: Reactome

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.59. 2681.
ReactomeiR-HSA-446210. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-D-glucosamine kinase (EC:2.7.1.59)
Short name:
N-acetylglucosamine kinase
Alternative name(s):
GlcNAc kinase
Gene namesi
Name:NAGK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000124357.12.
HGNCiHGNC:17174. NAGK.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi55577.
OpenTargetsiENSG00000124357.
PharmGKBiPA31436.

Chemistry databases

DrugBankiDB00141. N-Acetyl-D-glucosamine.

Polymorphism and mutation databases

BioMutaiNAGK.
DMDMi24638065.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000966962 – 344N-acetyl-D-glucosamine kinaseAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei205Phosphotyrosine1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UJ70.
PaxDbiQ9UJ70.
PeptideAtlasiQ9UJ70.
PRIDEiQ9UJ70.

2D gel databases

OGPiQ9UJ70.

PTM databases

iPTMnetiQ9UJ70.
PhosphoSitePlusiQ9UJ70.
SwissPalmiQ9UJ70.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000124357.
CleanExiHS_NAGK.
ExpressionAtlasiQ9UJ70. baseline and differential.
GenevisibleiQ9UJ70. HS.

Organism-specific databases

HPAiHPA035206.
HPA035207.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi120728. 93 interactors.
IntActiQ9UJ70. 39 interactors.
MINTiMINT-1182067.
STRINGi9606.ENSP00000389087.

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi15 – 21Combined sources7
Beta strandi26 – 32Combined sources7
Helixi37 – 40Combined sources4
Helixi42 – 60Combined sources19
Beta strandi64 – 66Combined sources3
Beta strandi68 – 75Combined sources8
Turni76 – 79Combined sources4
Helixi81 – 94Combined sources14
Beta strandi98 – 100Combined sources3
Beta strandi102 – 106Combined sources5
Helixi107 – 115Combined sources9
Beta strandi120 – 134Combined sources15
Beta strandi136 – 138Combined sources3
Beta strandi140 – 144Combined sources5
Turni148 – 150Combined sources3
Helixi156 – 171Combined sources16
Helixi182 – 192Combined sources11
Helixi197 – 201Combined sources5
Turni202 – 207Combined sources6
Helixi210 – 214Combined sources5
Helixi217 – 225Combined sources9
Helixi229 – 249Combined sources21
Helixi250 – 252Combined sources3
Helixi255 – 258Combined sources4
Beta strandi259 – 262Combined sources4
Beta strandi264 – 270Combined sources7
Helixi271 – 275Combined sources5
Helixi276 – 290Combined sources15
Beta strandi300 – 309Combined sources10
Helixi312 – 321Combined sources10
Turni322 – 324Combined sources3
Helixi331 – 334Combined sources4
Beta strandi335 – 342Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CH5X-ray1.90A/B/C/D1-344[»]
2CH6X-ray2.72A/B/C/D1-344[»]
ProteinModelPortaliQ9UJ70.
SMRiQ9UJ70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UJ70.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 130Substrate bindingCombined sources1 Publication2
Regioni145 – 147Substrate bindingCombined sources1 Publication3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1794. Eukaryota.
COG2971. LUCA.
GeneTreeiENSGT00510000047418.
HOGENOMiHOG000007248.
HOVERGENiHBG052570.
InParanoidiQ9UJ70.
KOiK00884.
OMAiLLPMDYS.
OrthoDBiEOG091G0LXR.
PhylomeDBiQ9UJ70.
TreeFamiTF314158.

Family and domain databases

InterProiView protein in InterPro
IPR002731. ATPase_BadF.
PfamiView protein in Pfam
PF01869. BcrAD_BadFG. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UJ70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAIYGGVEG GGTRSEVLLV SEDGKILAEA DGLSTNHWLI GTDKCVERIN
60 70 80 90 100
EMVNRAKRKA GVDPLVPLRS LGLSLSGGDQ EDAGRILIEE LRDRFPYLSE
110 120 130 140 150
SYLITTDAAG SIATATPDGG VVLISGTGSN CRLINPDGSE SGCGGWGHMM
160 170 180 190 200
GDEGSAYWIA HQAVKIVFDS IDNLEAAPHD IGYVKQAMFH YFQVPDRLGI
210 220 230 240 250
LTHLYRDFDK CRFAGFCRKI AEGAQQGDPL SRYIFRKAGE MLGRHIVAVL
260 270 280 290 300
PEIDPVLFQG KIGLPILCVG SVWKSWELLK EGFLLALTQG REIQAQNFFS
310 320 330 340
SFTLMKLRHS SALGGASLGA RHIGHLLPMD YSANAIAFYS YTFS
Length:344
Mass (Da):37,376
Last modified:January 23, 2007 - v4
Checksum:iFCBB6B328EF4D515
GO
Isoform 2 (identifier: Q9UJ70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRTRTGSQLAAREVTGSGAVPRQLEGRRCQAGRDANGGTSSDGSSSM

Show »
Length:390
Mass (Da):42,038
Checksum:i2B2DD636C8C35ADF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70S → I in CAB61848 (PubMed:10824116).Curated1
Sequence conflicti121V → I in CAB61848 (PubMed:10824116).Curated1
Sequence conflicti211C → Y in BAD96365 (Ref. 4) Curated1
Sequence conflicti286A → V in BAA91923 (PubMed:14702039).Curated1
Sequence conflicti324G → R in BAA91923 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02976338W → R1 PublicationCorresponds to variant dbSNP:rs17856147Ensembl.1
Natural variantiVAR_02976460A → V1 PublicationCorresponds to variant dbSNP:rs17849984Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0445861M → MRTRTGSQLAAREVTGSGAV PRQLEGRRCQAGRDANGGTS SDGSSSM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242910 mRNA. Translation: CAB61848.1.
AK001812 mRNA. Translation: BAA91923.1.
AK297224 mRNA. Translation: BAG59707.1.
CR457271 mRNA. Translation: CAG33552.1.
AK222645 mRNA. Translation: BAD96365.1.
AC007881 Genomic DNA. Translation: AAY14748.1.
CH471053 Genomic DNA. Translation: EAW99780.1.
BC001029 mRNA. Translation: AAH01029.1.
BC005371 mRNA. Translation: AAH05371.1.
CCDSiCCDS33220.2. [Q9UJ70-2]
RefSeqiNP_060037.3. NM_017567.4. [Q9UJ70-2]
UniGeneiHs.7036.

Genome annotation databases

EnsembliENST00000244204; ENSP00000244204; ENSG00000124357. [Q9UJ70-1]
ENST00000455662; ENSP00000389087; ENSG00000124357. [Q9UJ70-2]
ENST00000613852; ENSP00000477639; ENSG00000124357. [Q9UJ70-2]
GeneIDi55577.
KEGGihsa:55577.
UCSCiuc002shp.4. human. [Q9UJ70-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNAGK_HUMAN
AccessioniPrimary (citable) accession number: Q9UJ70
Secondary accession number(s): B4DLZ5
, Q53HD5, Q6IA84, Q9BS29, Q9BVP0, Q9NV37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 155 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families