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Protein

Gamma-glutamyltransferase 7

Gene

GGT7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves glutathione conjugates.By similarity

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
Glutathione + H2O = L-cysteinylglycine + L-glutamate.

Pathwayi

GO - Molecular functioni

  1. gamma-glutamyltransferase activity Source: UniProtKB
  2. glutathione hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutathione biosynthetic process Source: UniProtKB-KW
  2. glutathione metabolic process Source: UniProtKB
  3. leukotriene biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Glutathione biosynthesis

Enzyme and pathway databases

ReactomeiREACT_228214. Aflatoxin activation and detoxification.
REACT_6960. Glutathione synthesis and recycling.
UniPathwayiUPA00204.

Protein family/group databases

MEROPSiT03.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyltransferase 7 (EC:2.3.2.2)
Short name:
GGT 7
Alternative name(s):
Gamma-glutamyltransferase-like 3
Gamma-glutamyltransferase-like 5
Gamma-glutamyltranspeptidase 7
Glutathione hydrolase 7 (EC:3.4.19.13)
Cleaved into the following 2 chains:
Gene namesi
Name:GGT7
Synonyms:GGTL3, GGTL5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:4259. GGT7.

Subcellular locationi

Membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 106106CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei107 – 12721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini128 – 662535ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28669.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Gamma-glutamyltransferase 7 heavy chainBy similarityPRO_0000011066Add
BLAST
Chaini473 – 662190Gamma-glutamyltransferase 7 light chainBy similarityPRO_0000011067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi586 – 5861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9UJ14.
PaxDbiQ9UJ14.
PRIDEiQ9UJ14.

PTM databases

PhosphoSiteiQ9UJ14.

Expressioni

Tissue specificityi

Widely expressed, but at low level, except in the airway epithelial cells. Detected in brain, heart, kidney, liver, lung, spleen, testis and trachea.

Gene expression databases

BgeeiQ9UJ14.
CleanExiHS_GGT7.
ExpressionAtlasiQ9UJ14. baseline and differential.
GenevestigatoriQ9UJ14.

Organism-specific databases

HPAiHPA013204.

Interactioni

Subunit structurei

Heterodimer composed of the light and heavy chains. The active site is located in the light chain (By similarity). Isoform 3 interacts with FAM57A.By similarity1 Publication

Protein-protein interaction databases

BioGridi108953. 7 interactions.
IntActiQ9UJ14. 1 interaction.
STRINGi9606.ENSP00000338964.

Structurei

3D structure databases

ProteinModelPortaliQ9UJ14.
SMRiQ9UJ14. Positions 133-655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0405.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000231793.
HOVERGENiHBG039468.
InParanoidiQ9UJ14.
KOiK00681.
OMAiMLVHDIR.
OrthoDBiEOG7DNNTR.
PhylomeDBiQ9UJ14.
TreeFamiTF333329.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UJ14-1) [UniParc]FASTAAdd to Basket

Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAENEASQE SALGAYSPVD YMSITSFPRL PEDEPAPAAP LRGRKDEDAF
60 70 80 90 100
LGDPDTDPDS FLKSARLQRL PSSSSEMGSQ DGSPLRETRK DPFSAAAAEC
110 120 130 140 150
SCRQDGLTVI VTACLTFATG VTVALVMQIY FGDPQIFQQG AVVTDAARCT
160 170 180 190 200
SLGIEVLSKQ GSSVDAAVAA ALCLGIVAPH SSGLGGGGVM LVHDIRRNES
210 220 230 240 250
HLIDFRESAP GALREETLQR SWETKPGLLV GVPGMVKGLH EAHQLYGRLP
260 270 280 290 300
WSQVLAFAAA VAQDGFNVTH DLARALAEQL PPNMSERFRE TFLPSGRPPL
310 320 330 340 350
PGSLLHRPDL AEVLDVLGTS GPAAFYAGGN LTLEMVAEAQ HAGGVITEED
360 370 380 390 400
FSNYSALVEK PVCGVYRGHL VLSPPPPHTG PALISALNIL EGFNLTSLVS
410 420 430 440 450
REQALHWVAE TLKIALALAS RLGDPVYDST ITESMDDMLS KVEAAYLRGH
460 470 480 490 500
INDSQAAPAP LLPVYELDGA PTAAQVLIMG PDDFIVAMVS SLNQPFGSGL
510 520 530 540 550
ITPSGILLNS QMLDFSWPNR TANHSAPSLE NSVQPGKRPL SFLLPTVVRP
560 570 580 590 600
AEGLCGTYLA LGANGAARGL SGLTQVLLNV LTLNRNLSDS LARGRLHPDL
610 620 630 640 650
QSNLLQVDSE FTEEEIEFLE ARGHHVEKVD VLSWVHGSRR TNNFIIAVKD
660
PRSPDAAGAT IL

Note: Gene prediction confirmed by EST data.

Length:662
Mass (Da):70,467
Last modified:July 10, 2007 - v2
Checksum:i29DDBC719CADD195
GO
Isoform 2 (identifier: Q9UJ14-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.
     273-289: ARALAEQLPPNMSERFR → GQWGLGIWERHEVDGEG
     290-662: Missing.

Note: No experimental confirmation available.

Show »
Length:213
Mass (Da):22,494
Checksum:i830752C08248C1DA
GO
Isoform 3 (identifier: Q9UJ14-5) [UniParc]FASTAAdd to Basket

Also known as: GGTL3B, B

The sequence of this isoform differs from the canonical sequence as follows:
     226-251: PGLLVGVPGMVKGLHEAHQLYGRLPW → VGTLVRRESSGESLFIALLLTQALIC
     252-662: Missing.

Show »
Length:251
Mass (Da):26,578
Checksum:i3A8BBC5A2B81311C
GO
Isoform 4 (identifier: Q9UJ14-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-283: Missing.
     369-425: HLVLSPPPPH...LALASRLGDP → DLSPGSQGPP...QTRVSPPSEK
     426-662: Missing.

Note: No experimental confirmation available.

Show »
Length:142
Mass (Da):15,236
Checksum:i15B48378E9DCA3A5
GO

Sequence cautioni

The sequence BAC03394.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51N → K in AAN15928. (PubMed:12270127)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 283283Missing in isoform 4. 1 PublicationVSP_008133Add
BLAST
Alternative sequencei1 – 7676Missing in isoform 2. 1 PublicationVSP_008134Add
BLAST
Alternative sequencei226 – 25126PGLLV…GRLPW → VGTLVRRESSGESLFIALLL TQALIC in isoform 3. 1 PublicationVSP_008136Add
BLAST
Alternative sequencei252 – 662411Missing in isoform 3. 1 PublicationVSP_008137Add
BLAST
Alternative sequencei273 – 28917ARALA…SERFR → GQWGLGIWERHEVDGEG in isoform 2. 1 PublicationVSP_008138Add
BLAST
Alternative sequencei290 – 662373Missing in isoform 2. 1 PublicationVSP_008139Add
BLAST
Alternative sequencei369 – 42557HLVLS…RLGDP → DLSPGSQGPPSGEASQSMAT SFWPRDSSPFHRRETKAVSY LGSQLLKQTRVSPPSEK in isoform 4. 1 PublicationVSP_008140Add
BLAST
Alternative sequencei426 – 662237Missing in isoform 4. 1 PublicationVSP_008141Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY138815 mRNA. Translation: AAN15928.1.
AK097100 mRNA. Translation: BAC04950.1.
AK090413 mRNA. Translation: BAC03394.1. Different initiation.
AL049709 Genomic DNA. Translation: CAB61787.2.
CCDSiCCDS13242.2. [Q9UJ14-1]
RefSeqiNP_821158.2. NM_178026.2. [Q9UJ14-1]
UniGeneiHs.433738.

Genome annotation databases

EnsembliENST00000336431; ENSP00000338964; ENSG00000131067. [Q9UJ14-1]
GeneIDi2686.
KEGGihsa:2686.
UCSCiuc002xay.3. human. [Q9UJ14-1]
uc002xba.1. human. [Q9UJ14-5]

Polymorphism databases

DMDMi152031612.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY138815 mRNA. Translation: AAN15928.1.
AK097100 mRNA. Translation: BAC04950.1.
AK090413 mRNA. Translation: BAC03394.1. Different initiation.
AL049709 Genomic DNA. Translation: CAB61787.2.
CCDSiCCDS13242.2. [Q9UJ14-1]
RefSeqiNP_821158.2. NM_178026.2. [Q9UJ14-1]
UniGeneiHs.433738.

3D structure databases

ProteinModelPortaliQ9UJ14.
SMRiQ9UJ14. Positions 133-655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108953. 7 interactions.
IntActiQ9UJ14. 1 interaction.
STRINGi9606.ENSP00000338964.

Protein family/group databases

MEROPSiT03.017.

PTM databases

PhosphoSiteiQ9UJ14.

Polymorphism databases

DMDMi152031612.

Proteomic databases

MaxQBiQ9UJ14.
PaxDbiQ9UJ14.
PRIDEiQ9UJ14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336431; ENSP00000338964; ENSG00000131067. [Q9UJ14-1]
GeneIDi2686.
KEGGihsa:2686.
UCSCiuc002xay.3. human. [Q9UJ14-1]
uc002xba.1. human. [Q9UJ14-5]

Organism-specific databases

CTDi2686.
GeneCardsiGC20M033435.
H-InvDBHIX0203047.
HGNCiHGNC:4259. GGT7.
HPAiHPA013204.
MIMi612342. gene.
neXtProtiNX_Q9UJ14.
PharmGKBiPA28669.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0405.
GeneTreeiENSGT00550000074591.
HOGENOMiHOG000231793.
HOVERGENiHBG039468.
InParanoidiQ9UJ14.
KOiK00681.
OMAiMLVHDIR.
OrthoDBiEOG7DNNTR.
PhylomeDBiQ9UJ14.
TreeFamiTF333329.

Enzyme and pathway databases

UniPathwayiUPA00204.
ReactomeiREACT_228214. Aflatoxin activation and detoxification.
REACT_6960. Glutathione synthesis and recycling.

Miscellaneous databases

ChiTaRSiGGT7. human.
GeneWikiiGGTL3.
GenomeRNAii2686.
NextBioi10602.
PROiQ9UJ14.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UJ14.
CleanExiHS_GGT7.
ExpressionAtlasiQ9UJ14. baseline and differential.
GenevestigatoriQ9UJ14.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of CT120, a novel membrane-associated gene involved in amino acid transport and glutathione metabolism."
    He X.H., Di Y., Li J., Xie Y., Tang Y., Zhang F., Wei L., Zhang Y., Qin W.X., Huo K., Li Y., Wan D.F., Gu J.R.
    Biochem. Biophys. Res. Commun. 297:528-536(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH FAM57A.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Spleen.
  3. "Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones."
    Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N., Ohara O.
    DNA Res. 10:49-57(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Spleen.
  4. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NOMENCLATURE.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGGT7_HUMAN
AccessioniPrimary (citable) accession number: Q9UJ14
Secondary accession number(s): Q8N899
, Q8NF66, Q9BYP5, Q9BYP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: July 10, 2007
Last modified: February 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.