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Protein

Kinesin-like protein KIF25

Gene

KIF25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Minus-end microtubule-dependent motor protein (By similarity). Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase (PubMed:28263957). Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis (PubMed:28263957). May also act as a negative regulator of amino acid starvation-induced autophagy (PubMed:22354037).By similarity2 Publications

Caution

In contrast to the ortholog protein in primates, human KIF25 protein is shorter at the N-terminus. While the kinesin motor domain is intact, it is unknown whether the absence of the N-terminus affects the microtubule-dependent motor activity.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 72ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • establishment of spindle orientation Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • mitotic sister chromatid segregation Source: ProtInc
  • negative regulation of autophagy Source: BHF-UCL
  • negative regulation of mitotic centrosome separation Source: UniProtKB
  • nucleus localization Source: UniProtKB
  • organelle organization Source: ProtInc
  • protein homotetramerization Source: UniProtKB

Keywordsi

Molecular functionMotor protein
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF25Curated
Alternative name(s):
Kinesin-like protein 31 Publication
Gene namesi
Name:KIF25Imported
Synonyms:KNSL31 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000125337.16
HGNCiHGNC:6390 KIF25
MIMi603815 gene
neXtProtiNX_Q9UIL4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000125337
PharmGKBiPA30179

Polymorphism and mutation databases

BioMutaiKIF25
DMDMi20138788

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001254351 – 384Kinesin-like protein KIF25Add BLAST384

Proteomic databases

PaxDbiQ9UIL4
PeptideAtlasiQ9UIL4
PRIDEiQ9UIL4

PTM databases

iPTMnetiQ9UIL4
PhosphoSitePlusiQ9UIL4

Expressioni

Gene expression databases

BgeeiENSG00000125337
CleanExiHS_KIF25
ExpressionAtlasiQ9UIL4 baseline and differential
GenevisibleiQ9UIL4 HS

Organism-specific databases

HPAiHPA046153

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110032, 1 interactor
IntActiQ9UIL4, 1 interactor
STRINGi9606.ENSP00000346401

Structurei

3D structure databases

ProteinModelPortaliQ9UIL4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 363Kinesin motorPROSITE-ProRule annotationAdd BLAST357

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0239 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00910000144010
HOGENOMiHOG000203153
HOVERGENiHBG052254
InParanoidiQ9UIL4
KOiK18627
OMAiSTADQAC
OrthoDBiEOG091G04L9
PhylomeDBiQ9UIL4
TreeFamiTF105234

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UIL4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTWTSGQLQR EKQARPGSGA VLAFPDDKDL RVYGPAESQS AVFGDVCPLL
60 70 80 90 100
TSLLDGYNVC VMAYGQTGSG KSYTMLGRHS DDGPVLPLDP QSDLGIIPRV
110 120 130 140 150
AEELFRLILE NTSRSPKVEV SIVEVYNNDI FDLLAKDSIA AVSGVKREVV
160 170 180 190 200
TAKDGRTEVA LLASEAVGSA SKLMELVHGG LQLRAKHPTL VHADSSRSHL
210 220 230 240 250
IITVTLTTAS CSDSTADQAC SATLPREQTE AGRAGRSRRA SQGALAPQLV
260 270 280 290 300
PGNPAGHAEQ VQARLQLVDS AGSECVGVSG VTGLALREMA CISRSLAALA
310 320 330 340 350
GVLGALLEHR GHAPYRNSRL THLLQDCLGG DAKLLVILCI SPSQRHLAQT
360 370 380
LQGLGFGIRA RQVQRGPARK KPPSSQTEGK RRPD
Length:384
Mass (Da):40,686
Last modified:January 23, 2002 - v2
Checksum:i7739D463BCFDB429
GO
Isoform 2 (identifier: Q9UIL4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-329: Missing.

Show »
Length:332
Mass (Da):35,235
Checksum:i54CDE14CC3278A5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185A → T in BAF82177 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05936928K → M1 PublicationCorresponds to variant dbSNP:rs4708626Ensembl.1
Natural variantiVAR_06128041A → T. Corresponds to variant dbSNP:rs34049091Ensembl.1
Natural variantiVAR_049687229T → P. Corresponds to variant dbSNP:rs12197062Ensembl.1
Natural variantiVAR_049688255A → T. Corresponds to variant dbSNP:rs2073634Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002867278 – 329Missing in isoform 2. CuratedAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012722 mRNA Translation: BAA36417.1
AK289488 mRNA Translation: BAF82177.1
AB012723 Genomic DNA Translation: BAA36418.1
AL589733 Genomic DNA No translation available.
CCDSiCCDS43530.1 [Q9UIL4-2]
CCDS5305.1 [Q9UIL4-1]
RefSeqiNP_005346.3, NM_005355.3 [Q9UIL4-2]
NP_085118.2, NM_030615.2 [Q9UIL4-1]
XP_011534104.1, XM_011535802.2 [Q9UIL4-1]
XP_011534105.1, XM_011535803.2 [Q9UIL4-2]
UniGeneiHs.150013

Genome annotation databases

EnsembliENST00000351261; ENSP00000252688; ENSG00000125337 [Q9UIL4-2]
ENST00000354419; ENSP00000346401; ENSG00000125337 [Q9UIL4-1]
ENST00000443060; ENSP00000388878; ENSG00000125337 [Q9UIL4-1]
GeneIDi3834
KEGGihsa:3834
UCSCiuc003qwk.1 human [Q9UIL4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKIF25_HUMAN
AccessioniPrimary (citable) accession number: Q9UIL4
Secondary accession number(s): A8K0C3, O94775, Q5SZU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2002
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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