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Protein

GTP:AMP phosphotransferase AK3, mitochondrial

Gene

AK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.UniRule annotation1 Publication

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38AMPUniRule annotation1
Binding sitei43AMPUniRule annotation1
Binding sitei98AMPUniRule annotation1
Binding sitei128GTPUniRule annotation1
Binding sitei161AMPUniRule annotation1
Binding sitei172AMPUniRule annotation1
Binding sitei201GTP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 22GTPUniRule annotation6
Nucleotide bindingi64 – 66AMPUniRule annotation3
Nucleotide bindingi91 – 94AMPUniRule annotation4
Nucleotide bindingi137 – 138GTPUniRule annotation2

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • nucleoside triphosphate adenylate kinase activity Source: BHF-UCL

GO - Biological processi

  • ADP biosynthetic process Source: UniProtKB-HAMAP
  • AMP metabolic process Source: BHF-UCL
  • blood coagulation Source: Reactome
  • GTP metabolic process Source: BHF-UCL
  • ITP metabolic process Source: BHF-UCL
  • UTP metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS07472-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase AK3, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Adenylate kinase 3 alpha-like 1UniRule annotation
Gene namesi
Name:AK3UniRule annotation
Synonyms:AK3L1, AK6, AKL3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:17376. AK3.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: BHF-UCL
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi50808.
OpenTargetsiENSG00000147853.
PharmGKBiPA164741184.

Polymorphism and mutation databases

BioMutaiAK3.
DMDMi23831297.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001589222 – 227GTP:AMP phosphotransferase AK3, mitochondrialAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20N6-succinyllysineBy similarity1
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei57N6-succinyllysineBy similarity1
Modified residuei64N6-acetyllysine; alternateBy similarity1
Modified residuei64N6-succinyllysine; alternateBy similarity1
Modified residuei80N6-acetyllysine; alternateBy similarity1
Modified residuei80N6-succinyllysine; alternateBy similarity1
Modified residuei174N6-acetyllysine; alternateBy similarity1
Modified residuei174N6-succinyllysine; alternateBy similarity1
Modified residuei189N6-acetyllysine; alternateBy similarity1
Modified residuei189N6-succinyllysine; alternateBy similarity1
Modified residuei203N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UIJ7.
PaxDbiQ9UIJ7.
PeptideAtlasiQ9UIJ7.
PRIDEiQ9UIJ7.
TopDownProteomicsiQ9UIJ7-1. [Q9UIJ7-1]
Q9UIJ7-3. [Q9UIJ7-3]

2D gel databases

OGPiQ9UIJ7.
REPRODUCTION-2DPAGEIPI00465256.
UCD-2DPAGEQ9UIJ7.

PTM databases

iPTMnetiQ9UIJ7.
PhosphoSitePlusiQ9UIJ7.

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle and liver, moderately expressed in pancreas and kidney, and weakly expressed in placenta, brain and lung.1 Publication

Gene expression databases

BgeeiENSG00000147853.
CleanExiHS_AK3.
HS_AK3L1.
GenevisibleiQ9UIJ7. HS.

Organism-specific databases

HPAiHPA063324.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
APPBP2Q926245EBI-3916527,EBI-743771

Protein-protein interaction databases

BioGridi119127. 8 interactors.
IntActiQ9UIJ7. 6 interactors.
STRINGi9606.ENSP00000371230.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 14Combined sources6
Helixi20 – 30Combined sources11
Beta strandi31 – 37Combined sources7
Helixi38 – 48Combined sources11
Helixi51 – 60Combined sources10
Turni61 – 63Combined sources3
Helixi68 – 80Combined sources13
Beta strandi87 – 91Combined sources5
Helixi96 – 103Combined sources8
Beta strandi110 – 115Combined sources6
Helixi118 – 125Combined sources8
Beta strandi128 – 131Combined sources4
Turni132 – 135Combined sources4
Beta strandi136 – 139Combined sources4
Turni140 – 142Combined sources3
Turni152 – 154Combined sources3
Helixi162 – 164Combined sources3
Helixi166 – 189Combined sources24
Beta strandi193 – 197Combined sources5
Helixi201 – 212Combined sources12
Turni213 – 215Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZD8X-ray1.48A1-227[»]
ProteinModelPortaliQ9UIJ7.
SMRiQ9UIJ7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UIJ7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 66NMPbindUniRule annotation1 PublicationAdd BLAST30
Regioni127 – 164LIDUniRule annotation1 PublicationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiQ9UIJ7.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
PhylomeDBiQ9UIJ7.
TreeFamiTF312916.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UIJ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGASARLLRA VIMGAPGSGK GTVSSRITTH FELKHLSSGD LLRDNMLRGT
60 70 80 90 100
EIGVLAKAFI DQGKLIPDDV MTRLALHELK NLTQYSWLLD GFPRTLPQAE
110 120 130 140 150
ALDRAYQIDT VINLNVPFEV IKQRLTARWI HPASGRVYNI EFNPPKTVGI
160 170 180 190 200
DDLTGEPLIQ REDDKPETVI KRLKAYEDQT KPVLEYYQKK GVLETFSGTE
210 220
TNKIWPYVYA FLQTKVPQRS QKASVTP
Length:227
Mass (Da):25,565
Last modified:January 23, 2007 - v4
Checksum:i98A0EDF4FD9C9CEF
GO
Isoform 2 (identifier: Q9UIJ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Show »
Length:157
Mass (Da):18,192
Checksum:iF7D36803A8A241A4
GO
Isoform 3 (identifier: Q9UIJ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-90: Missing.

Note: No experimental confirmation available.
Show »
Length:187
Mass (Da):21,014
Checksum:i107B098298461090
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5A → G in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti38S → R in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti57K → Q in BAA87913 (PubMed:11485571).Curated1
Sequence conflicti69 – 71DVM → YVT in BAA87913 (PubMed:11485571).Curated3
Sequence conflicti181K → E in BAG60470 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0430901 – 70Missing in isoform 2. 2 PublicationsAdd BLAST70
Alternative sequenceiVSP_04487651 – 90Missing in isoform 3. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021870 mRNA. Translation: BAA87913.1.
AF183419 mRNA. Translation: AAG09688.1.
AK001553 mRNA. Translation: BAA91753.1.
AK001951 mRNA. Translation: BAA91996.1.
AK027534 mRNA. Translation: BAB55183.1.
AK098205 mRNA. Translation: BAG53592.1.
AK298200 mRNA. Translation: BAG60470.1.
AL136231, AL353151 Genomic DNA. Translation: CAI41262.1.
AL353151, AL136231 Genomic DNA. Translation: CAH72282.1.
AL136231, AL353151 Genomic DNA. Translation: CAI41260.1.
AL353151, AL136231 Genomic DNA. Translation: CAH72283.1.
CH471071 Genomic DNA. Translation: EAW58779.1.
CH471071 Genomic DNA. Translation: EAW58780.1.
CH471071 Genomic DNA. Translation: EAW58781.1.
BC013771 mRNA. Translation: AAH13771.1.
CCDSiCCDS56561.1. [Q9UIJ7-2]
CCDS56562.1. [Q9UIJ7-3]
CCDS6455.1. [Q9UIJ7-1]
RefSeqiNP_001186781.1. NM_001199852.1. [Q9UIJ7-3]
NP_001186782.1. NM_001199853.1. [Q9UIJ7-2]
NP_001186784.1. NM_001199855.1. [Q9UIJ7-2]
NP_001186785.1. NM_001199856.1. [Q9UIJ7-2]
NP_057366.2. NM_016282.3. [Q9UIJ7-1]
UniGeneiHs.732022.

Genome annotation databases

EnsembliENST00000359883; ENSP00000352948; ENSG00000147853. [Q9UIJ7-2]
ENST00000381809; ENSP00000371230; ENSG00000147853. [Q9UIJ7-1]
ENST00000447596; ENSP00000413933; ENSG00000147853. [Q9UIJ7-3]
ENST00000611749; ENSP00000482308; ENSG00000147853. [Q9UIJ7-2]
GeneIDi50808.
KEGGihsa:50808.
UCSCiuc003ziq.4. human. [Q9UIJ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021870 mRNA. Translation: BAA87913.1.
AF183419 mRNA. Translation: AAG09688.1.
AK001553 mRNA. Translation: BAA91753.1.
AK001951 mRNA. Translation: BAA91996.1.
AK027534 mRNA. Translation: BAB55183.1.
AK098205 mRNA. Translation: BAG53592.1.
AK298200 mRNA. Translation: BAG60470.1.
AL136231, AL353151 Genomic DNA. Translation: CAI41262.1.
AL353151, AL136231 Genomic DNA. Translation: CAH72282.1.
AL136231, AL353151 Genomic DNA. Translation: CAI41260.1.
AL353151, AL136231 Genomic DNA. Translation: CAH72283.1.
CH471071 Genomic DNA. Translation: EAW58779.1.
CH471071 Genomic DNA. Translation: EAW58780.1.
CH471071 Genomic DNA. Translation: EAW58781.1.
BC013771 mRNA. Translation: AAH13771.1.
CCDSiCCDS56561.1. [Q9UIJ7-2]
CCDS56562.1. [Q9UIJ7-3]
CCDS6455.1. [Q9UIJ7-1]
RefSeqiNP_001186781.1. NM_001199852.1. [Q9UIJ7-3]
NP_001186782.1. NM_001199853.1. [Q9UIJ7-2]
NP_001186784.1. NM_001199855.1. [Q9UIJ7-2]
NP_001186785.1. NM_001199856.1. [Q9UIJ7-2]
NP_057366.2. NM_016282.3. [Q9UIJ7-1]
UniGeneiHs.732022.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZD8X-ray1.48A1-227[»]
ProteinModelPortaliQ9UIJ7.
SMRiQ9UIJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119127. 8 interactors.
IntActiQ9UIJ7. 6 interactors.
STRINGi9606.ENSP00000371230.

PTM databases

iPTMnetiQ9UIJ7.
PhosphoSitePlusiQ9UIJ7.

Polymorphism and mutation databases

BioMutaiAK3.
DMDMi23831297.

2D gel databases

OGPiQ9UIJ7.
REPRODUCTION-2DPAGEIPI00465256.
UCD-2DPAGEQ9UIJ7.

Proteomic databases

EPDiQ9UIJ7.
PaxDbiQ9UIJ7.
PeptideAtlasiQ9UIJ7.
PRIDEiQ9UIJ7.
TopDownProteomicsiQ9UIJ7-1. [Q9UIJ7-1]
Q9UIJ7-3. [Q9UIJ7-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359883; ENSP00000352948; ENSG00000147853. [Q9UIJ7-2]
ENST00000381809; ENSP00000371230; ENSG00000147853. [Q9UIJ7-1]
ENST00000447596; ENSP00000413933; ENSG00000147853. [Q9UIJ7-3]
ENST00000611749; ENSP00000482308; ENSG00000147853. [Q9UIJ7-2]
GeneIDi50808.
KEGGihsa:50808.
UCSCiuc003ziq.4. human. [Q9UIJ7-1]

Organism-specific databases

CTDi50808.
DisGeNETi50808.
GeneCardsiAK3.
HGNCiHGNC:17376. AK3.
HPAiHPA063324.
MIMi609290. gene.
neXtProtiNX_Q9UIJ7.
OpenTargetsiENSG00000147853.
PharmGKBiPA164741184.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiQ9UIJ7.
KOiK00944.
OMAiNVPFQTI.
OrthoDBiEOG091G06BH.
PhylomeDBiQ9UIJ7.
TreeFamiTF312916.

Enzyme and pathway databases

BioCyciZFISH:HS07472-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSiAK3. human.
EvolutionaryTraceiQ9UIJ7.
GenomeRNAii50808.
PROiQ9UIJ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147853.
CleanExiHS_AK3.
HS_AK3L1.
GenevisibleiQ9UIJ7. HS.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03169. Adenylate_kinase_AK3. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57774. SSF57774. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD3_HUMAN
AccessioniPrimary (citable) accession number: Q9UIJ7
Secondary accession number(s): B4DP58
, D3DRI1, E7ET30, Q5VYW6, Q9H576, Q9HC01, Q9NPB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 159 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.