Q9UII6 (DUS13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 13
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
Dual specificity phosphatase SKRP4
Testis- and skeletal-muscle-specific DSP

Gene names

Name:	DUSP13
Synonyms:	TMDP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. Exhibits intrinsic phosphatase activity towards both phospho-seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Tissue specificity	Most abundantly expressed in the testis. Also found in the skeletal muscle. Testis-specific (at protein level). Ref.7
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative promoter usage Alternative splicing Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Traceable author statement Ref.1. Source: ProtInc peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC spermatogenesis Traceable author statement Ref.1. Source: ProtInc
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9UII6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by alternative promoter usage.

Isoform 3 (identifier: Q9UII6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGLPQPRLPA...TAWARYSHRM

Note: Produced by alternative promoter usage and alternative splicing. No experimental confirmation available.

Isoform 4 (identifier: Q9UII6-4)

Also known as: TMDP-L2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAETSLPELGGEDKATPCPSILELEELLRAGKSSCSRVDEVWPNLFIGDAM

Note: Produced by alternative promoter usage and alternative splicing. No experimental confirmation available.

Isoform 5 (identifier: Q6B8I1-1)

The sequence of this isoform can be found in the external entry Q6B8I1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage.

Isoform 6 (identifier: Q6B8I1-2)

The sequence of this isoform can be found in the external entry Q6B8I1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Isoform 7 (identifier: Q6B8I1-3)

The sequence of this isoform can be found in the external entry Q6B8I1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Isoform 8 (identifier: Q6B8I1-4)

The sequence of this isoform can be found in the external entry Q6B8I1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative promoter usage and alternative splicing. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

Dual specificity protein phosphatase 13

PRO_0000094820

Regions

Domain

114 – 183

70

Tyrosine-protein phosphatase

Sites

Active site

138

1

Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence

1

1

M → MGLPQPRLPAPALQAGPATA GCRPELRGQSWSLLPAMGLC HFATLALILLVLLEALAQAD TQKMVEAQRGVGPRACYSIW LLLAPTPPLSHCLQSPQKQH QVCGDRRLKAGSTNCPSEKC TAWARYSHRM in isoform 3.

VSP_037857

Alternative sequence

1

1

M → MAETSLPELGGEDKATPCPS ILELEELLRAGKSSCSRVDE VWPNLFIGDAM in isoform 4.

VSP_037858

Natural variant

62

1

R → Q.
Corresponds to variant rs16932004 [ dbSNP | Ensembl ].

VAR_057130

Natural variant

156

1

C → Y. Ref.3 Ref.4 Ref.6
Corresponds to variant rs3088142 [ dbSNP | Ensembl ].

VAR_025431

Natural variant

190

1

R → G.
Corresponds to variant rs16931996 [ dbSNP | Ensembl ].

VAR_057131

Experimental info

Sequence conflict

82

1

K → E in CAG33375. Ref.4

Secondary structure

1

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198

Helix Strand Turn

Details...

Helix

28 – 37

10

Beta strand

43 – 50

8

Beta strand

53 – 56

4

Helix

58 – 62

5

Helix

64 – 70

7

Beta strand

74 – 77

4

Helix

88 – 91

4

Turn

92 – 94

3

Beta strand

98 – 101

4

Helix

112 – 115

4

Helix

116 – 127

12

Beta strand

129 – 131

3

Beta strand

134 – 137

4

Beta strand

139 – 143

5

Helix

144 – 156

13

Helix

161 – 168

8

Turn

169 – 171

3

Helix

178 – 192

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 3. Checksum: 82EF05AB74E031EB	FASTA	198	22,149
	10 20 30 40 50 60 MDSLQKQDLR RPKIHGAVQA SPYQPPTLAS LQRLLWVRQA ATLNHIDEVW PSLFLGDAYA 70 80 90 100 110 120 ARDKSKLIQL GITHVVNAAA GKFQVDTGAK FYRGMSLEYY GIEADDNPFF DLSVYFLPVA 130 140 150 160 170 180 RYIRAALSVP QGRVLVHCAM GVSRSATLVL AFLMICENMT LVEAIQTVQA HRNICPNSGF 190 LRQLQVLDNR LGRETGRF
	Isoform 3 [UniParc]. Checksum: 8642DCF04F35334F	FASTA	327	36,079
	10 20 30 40 50 60 MGLPQPRLPA PALQAGPATA GCRPELRGQS WSLLPAMGLC HFATLALILL VLLEALAQAD 70 80 90 100 110 120 TQKMVEAQRG VGPRACYSIW LLLAPTPPLS HCLQSPQKQH QVCGDRRLKA GSTNCPSEKC 130 140 150 160 170 180 TAWARYSHRM DSLQKQDLRR PKIHGAVQAS PYQPPTLASL QRLLWVRQAA TLNHIDEVWP 190 200 210 220 230 240 SLFLGDAYAA RDKSKLIQLG ITHVVNAAAG KFQVDTGAKF YRGMSLEYYG IEADDNPFFD 250 260 270 280 290 300 LSVYFLPVAR YIRAALSVPQ GRVLVHCAMG VSRSATLVLA FLMICENMTL VEAIQTVQAH 310 320 RNICPNSGFL RQLQVLDNRL GRETGRF
	Isoform 4 (TMDP-L2) [UniParc]. Checksum: A92045D8C086F866	FASTA	248	27,507
	10 20 30 40 50 60 MAETSLPELG GEDKATPCPS ILELEELLRA GKSSCSRVDE VWPNLFIGDA MDSLQKQDLR 70 80 90 100 110 120 RPKIHGAVQA SPYQPPTLAS LQRLLWVRQA ATLNHIDEVW PSLFLGDAYA ARDKSKLIQL 130 140 150 160 170 180 GITHVVNAAA GKFQVDTGAK FYRGMSLEYY GIEADDNPFF DLSVYFLPVA RYIRAALSVP 190 200 210 220 230 240 QGRVLVHCAM GVSRSATLVL AFLMICENMT LVEAIQTVQA HRNICPNSGF LRQLQVLDNR LGRETGRF
	Isoform 5 [UniParc]. See Q6B8I1.		–	–
	Isoform 6 [UniParc]. See Q6B8I1.		–	–
	Isoform 7 [UniParc]. See Q6B8I1.		–	–
	Isoform 8 [UniParc]. See Q6B8I1.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel dual-specificity protein phosphatase possibly involved in spermatogenesis." Nakamura K., Shima H., Watanabe M., Haneji T., Kikuchi K. Biochem. J. 344:819-825(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION. Tissue: Skeletal muscle.
[2]	"Identification of a novel dual specificity phosphatase, SKRP4." Zama T., Aoki R., Murata M., Ikeda Y. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT TYR-156. Tissue: Skeletal muscle.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TYR-156.
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TYR-156. Tissue: Bone marrow.
[7]	"Characterization of two distinct dual specificity phosphatases encoded in alternative open reading frames of a single gene located on human chromosome 10q22.2." Chen H.-H., Luche R., Wei B., Tonks N.K. J. Biol. Chem. 279:41404-41413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.
[8]	"Crystal structure of human TMDP, a testis-specific dual specificity protein phosphatase: implications for substrate specificity." Kim S.J., Jeong D.G., Yoon T.S., Son J.H., Cho S.K., Ryu S.E., Kim J.H. Proteins 66:239-245(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB027004 mRNA. Translation: BAA89412.1.
AB103375 mRNA. Translation: BAD91014.1.
AK057012 mRNA. Translation: BAG51843.1.
AK300679 mRNA. Translation: BAG62362.1.
CR457094 mRNA. Translation: CAG33375.1.
AL392111 Genomic DNA. Translation: CAI40905.1.
BC009778 mRNA. Translation: AAH09778.1.

IPI

IPI00514257.
IPI00514507.
IPI00973092.
IPI01018872.

RefSeq

NP_001007272.1. NM_001007271.1.
NP_001007273.1. NM_001007272.1.
NP_001007274.1. NM_001007273.1.
NP_057448.3. NM_016364.3.

UniGene

Hs.178170.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GWO	X-ray	2.40	A/B/C/D	1-198	[»]
2PQ5	X-ray	2.30	A/B/C/D	1-198	[»]

ProteinModelPortal

Q9UII6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UII6. 1 interaction.

PTM databases

PhosphoSite

Q9UII6.

Polymorphism databases

DMDM

257051044.

Proteomic databases

PaxDb

Q9UII6.

PRIDE

Q9UII6.

Protocols and materials databases

DNASU

51207.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000308475; ENSP00000311051; ENSG00000079393.
ENST00000372700; ENSP00000361785; ENSG00000079393.
ENST00000394707; ENSP00000452702; ENSG00000079393.
ENST00000472493; ENSP00000444580; ENSG00000079393.

GeneID

51207.

KEGG

hsa:51207.

UCSC

uc001jwr.3. human.
uc001jwt.3. human.
uc001jww.3. human.

Organism-specific databases

CTD

51207.

GeneCards

GC10M076854.

HGNC

HGNC:19681. DUSP13.

HPA

HPA024162.

MIM

613191. gene.

neXtProt

NX_Q9UII6.

PharmGKB

PA134939640.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2453.

HOVERGEN

HBG001524.

InParanoid

Q9UII6.

KO

K14165.

OrthoDB

EOG402WSP.

PhylomeDB

Q9UII6.

Gene expression databases

ArrayExpress

Q9UII6.

Bgee

Q9UII6.

CleanEx

HS_DUSP13.

Genevestigator

Q9UII6.

GermOnline

ENSG00000079393. Homo sapiens.

Family and domain databases

InterPro

IPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]

PANTHER

PTHR10159. PTHR10159. 1 hit.

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

PRINTS

PR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9UII6.

GenomeRNAi

51207.

NextBio

54264.

SOURCE

Search...

Entry information

Entry name

DUS13_HUMAN

Accession

Primary (citable) accession number: Q9UII6
Secondary accession number(s): A8K776 , A8K782, B3KPY1, B3KXT0, B4DUK0, Q5JSC6, Q6IAR0, Q96GC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.