Q9UII2 (ATIF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATPase inhibitor, mitochondrial

Alternative name(s):
Inhibitor of F(1)F(o)-ATPase
Short name=IF(1)
Short name=IF1

Gene names

Name:	ATPIF1
Synonyms:	ATPI

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Ref.13
Subunit structure	Homodimer at a pH value below 6.5, inactive homotetramer above By similarity.
Subcellular location	Mitochondrion.
Post-translational modification	Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
Sequence similarities	Belongs to the ATPase inhibitor family.
Sequence caution	The sequence AAH04955.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transit peptide
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	angiogenesis Traceable author statement. Source: UniProtKB generation of precursor metabolites and energy Traceable author statement. Source: ProtInc negative regulation of endothelial cell proliferation Inferred from direct assay. Source: UniProtKB negative regulation of hydrolase activity Inferred from direct assay. Source: UniProtKB negative regulation of nucleotide metabolic process Inferred from electronic annotation. Source: InterPro protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cell surface Inferred from direct assay. Source: UniProtKB mitochondrion Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase binding Inferred from direct assay. Source: UniProtKB ATPase inhibitor activity Inferred from direct assay. Source: UniProtKB angiostatin binding Inferred from direct assay. Source: UniProtKB calmodulin binding Inferred from sequence or structural similarity. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UII2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UII2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     61-106: AQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD → HYRLCFEISLG

Isoform 3 (identifier: Q9UII2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     61-106: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Ref.12
	Chain	26 – 106	81	ATPase inhibitor, mitochondrial		PRO_0000002547
Regions
	Coiled coil	63 – 106	44	Potential
Natural variations
	Alternative sequence	61 – 106	46	AQSRE…LKHDD → HYRLCFEISLG in isoform 2.		VSP_041417
	Alternative sequence	61 – 106	46	Missing in isoform 3.		VSP_041418
Experimental info
	Sequence conflict	60	1	R → RR in CAI46227. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A6144431125D5A86	FASTA	106	12,249
	10 20 30 40 50 60 MAVTALAART WLGVWGVRTM QARGFGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR 70 80 90 100 AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD
	Isoform 2 [UniParc]. Checksum: D7E481A83975E005	FASTA	71	7,912
	10 20 30 40 50 60 MAVTALAART WLGVWGVRTM QARGFGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR 70 HYRLCFEISL G
	Isoform 3 [UniParc]. Checksum: 151FBFCD72F13D1C	FASTA	60	6,592
	10 20 30 40 50 60 MAVTALAART WLGVWGVRTM QARGFGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of cDNA coding the mitochondrial precursor protein of the ATPase inhibitor from humans." Ichikawa N., Ushida S., Kawabata M., Masazumi Y. Biosci. Biotechnol. Biochem. 63:2225-2227(1999) [PubMed: 10664857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Cloning and sequencing of a novel human cDNA homologous to bovine ATP synthase inhibitor protein mRNA." Dai F.Y., Yu L., Yang J., Zheng L.H., Wang X.K., Zhao S.Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"Cloning and characterization of a novel human gene ASI." Yu L., Zhang P., Gao J. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[6]	Li W.B., Gruber C., Jessee J., Polayes D. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Fetal liver.
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Uterus.
[12]	"Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini." Xu G., Shin S.B., Jaffrey S.R. Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009) [PubMed: 19892738] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 26-42, VARIABILITY IN MATURE CHAIN LENGTH. Tissue: Leukemic T-cell.
[13]	"IF(1): setting the pace of the F(1)F(o)-ATP synthase." Campanella M., Parker N., Tan C.H., Hall A.M., Duchen M.R. Trends Biochem. Sci. 34:343-350(2009) [PubMed: 19559621] [Abstract] Cited for: FUNCTION.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB029042 mRNA. Translation: BAA88422.1. AK316600 mRNA. Translation: BAG38187.1. DB030607 mRNA. No translation available. AL050386 mRNA. Translation: CAI46227.1. AF114836 mRNA. Translation: AAP97235.1. AY005470 Genomic DNA. Translation: AAF97495.1. AL583540 mRNA. No translation available. BT009849 mRNA. Translation: AAP88851.1. CR457097 mRNA. Translation: CAG33378.1. AL353622 Genomic DNA. Translation: CAI19134.1. CH471059 Genomic DNA. Translation: EAX07704.1. BC004955 mRNA. Translation: AAH04955.1. Different initiation. BC009677 mRNA. Translation: AAH09677.1.
IPI	IPI00258804. IPI00377040. IPI00553153.
PIR	JC7175.
RefSeq	NP_057395.1. NM_016311.4. NP_835497.1. NM_178190.2. NP_835498.1. NM_178191.2.
UniGene	Hs.726089.
3D structure databases
ProteinModelPortal	Q9UII2.
SMR	Q9UII2. Positions 45-103.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UII2. 8 interactions.
MINT	MINT-2878001.
STRING	Q9UII2.
PTM databases
PhosphoSite	Q9UII2.
Polymorphism databases
DMDM	12585262.
Proteomic databases
PRIDE	Q9UII2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335514; ENSP00000335203; ENSG00000130770.
GeneID	93974.
KEGG	hsa:93974.
UCSC	uc001bpq.1. human.
Organism-specific databases
CTD	93974.
GeneCards	GC01P028562.
H-InvDB	HIX0000295.
HGNC	HGNC:871. ATPIF1.
HPA	HPA027999.
neXtProt	NX_Q9UII2.
PharmGKB	PA25173.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000006264.
HOVERGEN	HBG061381.
InParanoid	Q9UII2.
OMA	RLGVWGV.
OrthoDB	EOG4X0MTZ.
PhylomeDB	Q9UII2.
Gene expression databases
ArrayExpress	Q9UII2.
Bgee	Q9UII2.
CleanEx	HS_ATPIF1.
Genevestigator	Q9UII2.
GermOnline	ENSG00000130770. Homo sapiens.
Family and domain databases
InterPro	IPR007648. ATPase_inhibitor_IATP_mt. [Graphical view]
Pfam	PF04568. IATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	78232.

Entry information

Entry name

ATIF1_HUMAN

Accession

Primary (citable) accession number: Q9UII2
Secondary accession number(s): Q5JXL8, Q6IAQ7, Q9BSL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.