Q9UID3 (VPS51_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 51 homolog

Alternative name(s):
Another new gene 2 protein
Protein fat-free homolog

Gene names

Name:	VPS51
Synonyms:	ANG2, C11orf2, C11orf3, FFR
ORF Names:	PP5382

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi networkl (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Ref.8
Subunit structure	Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts with STX6. Ref.8
Subcellular location	Golgi apparatus › trans-Golgi network Ref.8.
Sequence similarities	Belongs to the VPS51 family.

Ontologies

Keywords
Biological process	Lipid transport Protein transport Transport
Cellular component	Golgi apparatus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from mutant phenotype Ref.8. Source: UniProtKB lipid transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from direct assay Ref.8. Source: UniProtKB
Cellular_component	GARP complex Inferred from direct assay Ref.8. Source: UniProtKB integral to membrane Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UID3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UID3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-124: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 782	782	Vacuolar protein sorting-associated protein 51 homolog		PRO_0000089831
Regions
	Coiled coil	116 – 147	32	Potential
	Coiled coil	270 – 292	23	Potential
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 124	124	Missing in isoform 2.		VSP_014700

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 2. Checksum: 0E858672E4C656DD	FASTA	782	86,042
	10 20 30 40 50 60 MAAAAAAGPS PGSGPGDSPE GPEGEAPERR RKAHGMLKLY YGLSEGEAAG RPAGPDPLDP 70 80 90 100 110 120 TDLNGAHFDP EVYLDKLRRE CPLAQLMDSE TDMVRQIRAL DSDMQTLVYE NYNKFISATD 130 140 150 160 170 180 TIRKMKNDFR KMEDEMDRLA TNMAVITDFS ARISATLQDR HERITKLAGV HALLRKLQFL 190 200 210 220 230 240 FELPSRLTKC VELGAYGQAV RYQGRAQAVL QQYQHLPSFR AIQDDCQVIT ARLAQQLRQR 250 260 270 280 290 300 FREGGSGAPE QAECVELLLA LGEPAEELCE EFLAHARGRL EKELRNLEAE LGPSPPAPDV 310 320 330 340 350 360 LEFTDHGGSG FVGGLCQVAA AYQELFAAQG PAGAEKLAAF ARQLGSRYFA LVERRLAQEQ 370 380 390 400 410 420 GGGDNSLLVR ALDRFHRRLR APGALLAAAG LADAATEIVE RVARERLGHH LQGLRAAFLG 430 440 450 460 470 480 CLTDVRQALA APRVAGKEGP GLAELLANVA SSILSHIKAS LAAVHLFTAK EVSFSNKPYF 490 500 510 520 530 540 RGEFCSQGVR EGLIVGFVHS MCQTAQSFCD SPGEKGGATP PALLLLLSRL CLDYETATIS 550 560 570 580 590 600 YILTLTDEQF LVQDQFPVTP VSTLCAEARE TARRLLTHYV KVQGLVISQM LRKSVETRDW 610 620 630 640 650 660 LSTLEPRNVR AVMKRVVEDT TAIDVQVGLL YEEGVRKAQS SDSSKRTFSV YSSSRQQGRY 670 680 690 700 710 720 APSYTPSAPM DTNLLSNIQK LFSERIDVFS PVEFNKVSVL TGIIKISLKT LLECVRLRTF 730 740 750 760 770 780 GRFGLQQVQV DCHFLQLYLW RFVADEELVH LLLDEVVASA ALRCPDPVPM EPSVVEVICE RG
	Isoform 2 [UniParc]. Checksum: A887B9E4D045BAE6	FASTA	658	72,655
	10 20 30 40 50 60 MKNDFRKMED EMDRLATNMA VITDFSARIS ATLQDRHERI TKLAGVHALL RKLQFLFELP 70 80 90 100 110 120 SRLTKCVELG AYGQAVRYQG RAQAVLQQYQ HLPSFRAIQD DCQVITARLA QQLRQRFREG 130 140 150 160 170 180 GSGAPEQAEC VELLLALGEP AEELCEEFLA HARGRLEKEL RNLEAELGPS PPAPDVLEFT 190 200 210 220 230 240 DHGGSGFVGG LCQVAAAYQE LFAAQGPAGA EKLAAFARQL GSRYFALVER RLAQEQGGGD 250 260 270 280 290 300 NSLLVRALDR FHRRLRAPGA LLAAAGLADA ATEIVERVAR ERLGHHLQGL RAAFLGCLTD 310 320 330 340 350 360 VRQALAAPRV AGKEGPGLAE LLANVASSIL SHIKASLAAV HLFTAKEVSF SNKPYFRGEF 370 380 390 400 410 420 CSQGVREGLI VGFVHSMCQT AQSFCDSPGE KGGATPPALL LLLSRLCLDY ETATISYILT 430 440 450 460 470 480 LTDEQFLVQD QFPVTPVSTL CAEARETARR LLTHYVKVQG LVISQMLRKS VETRDWLSTL 490 500 510 520 530 540 EPRNVRAVMK RVVEDTTAID VQVGLLYEEG VRKAQSSDSS KRTFSVYSSS RQQGRYAPSY 550 560 570 580 590 600 TPSAPMDTNL LSNIQKLFSE RIDVFSPVEF NKVSVLTGII KISLKTLLEC VRLRTFGRFG 610 620 630 640 650 LQQVQVDCHF LQLYLWRFVA DEELVHLLLD EVVASAALRC PDPVPMEPSV VEVICERG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of TM7SF2 in the FAUNA gene cluster on human chromosome 11q13." Lemmens I.H., Kas K., Merregaert J., Van de Ven W.J.M. Genomics 49:437-442(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	Lemmens I.H., Kas K., Merregaert J., Van de Ven W.J.M. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix, Eye, Lung and Muscle.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 120-782 (ISOFORM 1). Tissue: Testis.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Ang2/fat-free is a conserved subunit of the Golgi-associated retrograde protein complex." Perez-Victoria F.J., Schindler C., Magadan J.G., Mardones G.A., Delevoye C., Romao M., Raposo G., Bonifacino J.S. Mol. Biol. Cell 21:3386-3395(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH VPS52; VPS53; VPS54 AND STX6.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF024631 mRNA. Translation: AAF21627.2. AF289557 mRNA. Translation: AAL55741.1. AL833818 mRNA. Translation: CAD38681.2. AP003068 Genomic DNA. No translation available. BC006555 mRNA. Translation: AAH06555.2. BC007198 mRNA. Translation: AAH07198.1. BC009285 mRNA. Translation: AAH09285.2. BC010540 mRNA. Translation: AAH10540.1. BC017438 mRNA. Translation: AAH17438.1.
IPI	IPI00001710. IPI00607717.
RefSeq	NP_037397.2. NM_013265.3.
UniGene	Hs.277517. Hs.732951.
3D structure databases
ProteinModelPortal	Q9UID3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UID3. 4 interactions.
STRING	9606.ENSP00000279281.
PTM databases
PhosphoSite	Q9UID3.
Polymorphism databases
DMDM	71153003.
Proteomic databases
PaxDb	Q9UID3.
PRIDE	Q9UID3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000279281; ENSP00000279281; ENSG00000149823.
GeneID	738.
KEGG	hsa:738.
UCSC	uc001ocr.1. human.
Organism-specific databases
CTD	738.
GeneCards	GC11P064856.
HGNC	HGNC:1172. VPS51.
HPA	HPA039650.
neXtProt	NX_Q9UID3.
PharmGKB	PA25485.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG127717.
HOGENOM	HOG000046877.
HOVERGEN	HBG107916.
InParanoid	Q9UID3.
OMA	NMAVITD.
OrthoDB	EOG49W2DW.
PhylomeDB	Q9UID3.
Gene expression databases
ArrayExpress	Q9UID3.
Bgee	Q9UID3.
CleanEx	HS_C11orf2.
Genevestigator	Q9UID3.
GermOnline	ENSG00000149823. Homo sapiens.
Family and domain databases
InterPro	IPR016159. Cullin_repeat-like_dom. IPR014812. Vps51. [Graphical view]
Pfam	PF08700. Vps51. 1 hit. [Graphical view]
SUPFAM	SSF74788. Cullin_repeat-like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	C11orf2. human.
GenomeRNAi	738.
NextBio	2996.

Entry information

Entry name

VPS51_HUMAN

Accession

Primary (citable) accession number: Q9UID3
Secondary accession number(s): Q6PJV5 , Q7L8A6, Q8WZ35, Q96DF4, Q96GR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.