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Protein

Leucine carboxyl methyltransferase 1

Gene

LCMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine = S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester.2 Publications

Kineticsi

  1. KM=0.1 µM for PP2AD1 Publication
  2. KM=1.3 µM for AdoMet1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei37S-adenosyl-L-methionineCombined sources1 Publication1
    Binding sitei73S-adenosyl-L-methionineCombined sources1 Publication1
    Binding sitei98S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication1
    Binding sitei122S-adenosyl-L-methionineCombined sources1 Publication1
    Binding sitei198S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • cellular protein modification process Source: ProtInc
    • C-terminal protein methylation Source: MGI
    • G2/M transition of mitotic cell cycle Source: Reactome
    • negative regulation of protein complex assembly Source: MGI
    • protein methylation Source: MGI
    • regulation of apoptotic process Source: MGI
    • regulation of glucose metabolic process Source: Ensembl
    • regulation of mitotic cell cycle spindle assembly checkpoint Source: MGI

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16510
    ReactomeiR-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition
    SABIO-RKiQ9UIC8

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine carboxyl methyltransferase 1 (EC:2.1.1.2332 Publications)
    Alternative name(s):
    Protein-leucine O-methyltransferase
    [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
    Gene namesi
    Name:LCMT1
    Synonyms:LCMT
    ORF Names:CGI-68
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 16

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000205629.11
    HGNCiHGNC:17557 LCMT1
    MIMi610286 gene
    neXtProtiNX_Q9UIC8

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi51451
    OpenTargetsiENSG00000205629
    PharmGKBiPA134928443

    Chemistry databases

    DrugBankiDB00149 L-Leucine

    Polymorphism and mutation databases

    BioMutaiLCMT1
    DMDMi12643251

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002044221 – 334Leucine carboxyl methyltransferase 1Add BLAST334

    Proteomic databases

    EPDiQ9UIC8
    PaxDbiQ9UIC8
    PeptideAtlasiQ9UIC8
    PRIDEiQ9UIC8

    PTM databases

    iPTMnetiQ9UIC8
    PhosphoSitePlusiQ9UIC8

    Expressioni

    Gene expression databases

    BgeeiENSG00000205629
    CleanExiHS_LCMT1
    ExpressionAtlasiQ9UIC8 baseline and differential
    GenevisibleiQ9UIC8 HS

    Organism-specific databases

    HPAiHPA041559
    HPA043530

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FXR2P511162EBI-747632,EBI-740459

    Protein-protein interaction databases

    BioGridi119549, 10 interactors
    IntActiQ9UIC8, 3 interactors
    MINTiQ9UIC8
    STRINGi9606.ENSP00000382021

    Structurei

    Secondary structure

    1334
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi28 – 43Combined sources16
    Helixi51 – 53Combined sources3
    Helixi64 – 87Combined sources24
    Turni88 – 90Combined sources3
    Beta strandi92 – 97Combined sources6
    Helixi104 – 110Combined sources7
    Beta strandi116 – 122Combined sources7
    Helixi124 – 136Combined sources13
    Helixi138 – 147Combined sources10
    Beta strandi148 – 152Combined sources5
    Beta strandi159 – 161Combined sources3
    Beta strandi163 – 169Combined sources7
    Helixi175 – 184Combined sources10
    Beta strandi193 – 199Combined sources7
    Helixi201 – 203Combined sources3
    Helixi206 – 219Combined sources14
    Beta strandi221 – 230Combined sources10
    Helixi236 – 246Combined sources11
    Turni247 – 249Combined sources3
    Helixi255 – 259Combined sources5
    Helixi261 – 269Combined sources9
    Turni270 – 272Combined sources3
    Beta strandi274 – 280Combined sources7
    Helixi281 – 286Combined sources6
    Helixi290 – 299Combined sources10
    Helixi305 – 312Combined sources8
    Beta strandi315 – 323Combined sources9
    Turni325 – 328Combined sources4
    Helixi329 – 331Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3IEIX-ray1.90A/B/C/D/E/F/G/H1-334[»]
    3O7WX-ray2.00A23-232[»]
    A259-334[»]
    3P71X-ray2.70T1-334[»]
    ProteinModelPortaliQ9UIC8
    SMRiQ9UIC8
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UIC8

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni171 – 172S-adenosyl-L-methionine bindingCombined sources1 Publication2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG2918 Eukaryota
    ENOG410YB9K LUCA
    GeneTreeiENSGT00530000063793
    HOVERGENiHBG052313
    InParanoidiQ9UIC8
    KOiK18203
    OMAiPYIQYFV
    OrthoDBiEOG091G0CW2
    PhylomeDBiQ9UIC8
    TreeFamiTF315087

    Family and domain databases

    InterProiView protein in InterPro
    IPR016651 Leu_CO_MeTrfase_LCMT1
    IPR007213 Ppm1/Ppm2/Tcmp
    IPR029063 SAM-dependent_MTases
    PfamiView protein in Pfam
    PF04072 LCM, 1 hit
    PIRSFiPIRSF016305 LCM_mtfrase, 1 hit
    SUPFAMiSSF53335 SSF53335, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9UIC8-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MATRQRESSI TSCCSTSSCD ADDEGVRGTC EDASLCKRFA VSIGYWHDPY
    60 70 80 90 100
    IQHFVRLSKE RKAPEINRGY FARVHGVSQL IKAFLRKTEC HCQIVNLGAG
    110 120 130 140 150
    MDTTFWRLKD EDLLPSKYFE VDFPMIVTRK LHSIKCKPPL SSPILELHSE
    160 170 180 190 200
    DTLQMDGHIL DSKRYAVIGA DLRDLSELEE KLKKCNMNTQ LPTLLIAECV
    210 220 230 240 250
    LVYMTPEQSA NLLKWAANSF ERAMFINYEQ VNMGDRFGQI MIENLRRRQC
    260 270 280 290 300
    DLAGVETCKS LESQKERLLS NGWETASAVD MMELYNRLPR AEVSRIESLE
    310 320 330
    FLDEMELLEQ LMRHYCLCWA TKGGNELGLK EITY
    Length:334
    Mass (Da):38,379
    Last modified:December 1, 2000 - v2
    Checksum:iC8D200118E6F353D
    GO
    Isoform 2 (identifier: Q9UIC8-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-37: MATRQRESSI...GTCEDASLCK → MLPCARELPS...CSHLGLRSGR

    Show »
    Length:357
    Mass (Da):41,106
    Checksum:iFBC25BB4C0B61057
    GO
    Isoform 3 (identifier: Q9UIC8-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         136-190: Missing.

    Show »
    Length:279
    Mass (Da):32,172
    Checksum:i6573F21B2405F64B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti49P → S in BAD97084 (Ref. 4) Curated1
    Sequence conflicti114L → P in BAB15270 (PubMed:14702039).Curated1
    Sequence conflicti248 – 249RQ → PS in AAD34063 (PubMed:10810093).Curated2
    Sequence conflicti251D → E in AAF18267 (PubMed:10600115).Curated1
    Sequence conflicti301F → L in BAB15270 (PubMed:14702039).Curated1
    Sequence conflicti306 – 307EL → DV in AAF18267 (PubMed:10600115).Curated2
    Sequence conflicti312 – 313MR → NA in AAF18267 (PubMed:10600115).Curated2

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0174281 – 37MATRQ…ASLCK → MLPCARELPSAAPDSTLLLT AQHWVANLFILATLIGWTVR SFFLGRMETCCSHLGLRSGR in isoform 2. 3 PublicationsAdd BLAST37
    Alternative sequenceiVSP_041416136 – 190Missing in isoform 3. CuratedAdd BLAST55

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF037601 mRNA Translation: AAF18267.1
    AF151826 mRNA Translation: AAD34063.1
    AK025884 mRNA Translation: BAB15270.1
    AK223364 mRNA Translation: BAD97084.1
    AK291885 mRNA Translation: BAF84574.1
    AK314409 mRNA Translation: BAG37031.1
    AC008741 Genomic DNA No translation available.
    AC133552 Genomic DNA No translation available.
    BC001214 mRNA Translation: AAH01214.1
    BC014217 mRNA Translation: AAH14217.1
    AL137283 mRNA Translation: CAB70677.1
    CCDSiCCDS45445.1 [Q9UIC8-1]
    CCDS45446.1 [Q9UIC8-3]
    PIRiT46352
    RefSeqiNP_001027563.1, NM_001032391.1 [Q9UIC8-3]
    NP_057393.2, NM_016309.2 [Q9UIC8-1]
    XP_011544166.1, XM_011545864.1 [Q9UIC8-2]
    UniGeneiHs.337730

    Genome annotation databases

    EnsembliENST00000380966; ENSP00000370353; ENSG00000205629 [Q9UIC8-3]
    ENST00000399069; ENSP00000382021; ENSG00000205629 [Q9UIC8-1]
    GeneIDi51451
    KEGGihsa:51451
    UCSCiuc002dnx.2 human [Q9UIC8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiLCMT1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UIC8
    Secondary accession number(s): A6NL89
    , A8K770, Q53FC5, Q96CI5, Q9H6I9, Q9NTG4, Q9Y378
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: April 25, 2018
    This is version 151 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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