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Q9UI40 (NCKX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/potassium/calcium exchanger 2
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 2
Retinal cone Na-Ca+K exchanger
Solute carrier family 24 member 2
Gene names
Name:SLC24A2
Synonyms:NCKX2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.

Ontologies

Keywords
   Biological processAntiport
Calcium transport
Ion transport
Potassium transport
Sensory transduction
Sodium transport
Symport
Transport
Vision
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandCalcium
Potassium
Sodium
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

ion transmembrane transport

Inferred from direct assay PubMed 18166528. Source: MGI

ion transport

Traceable author statement. Source: Reactome

learning

Inferred from electronic annotation. Source: Ensembl

long term synaptic depression

Inferred from electronic annotation. Source: Ensembl

long-term synaptic potentiation

Inferred from electronic annotation. Source: Ensembl

memory

Inferred from electronic annotation. Source: Ensembl

sodium ion transmembrane transport

Inferred from direct assay PubMed 18166528. Source: GOC

transmembrane transport

Traceable author statement. Source: Reactome

visual perception

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intrinsic component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functioncadmium ion binding

Inferred from electronic annotation. Source: Ensembl

calcium channel activity

Inferred from electronic annotation. Source: Ensembl

calcium ion binding

Inferred from electronic annotation. Source: Ensembl

calcium, potassium:sodium antiporter activity

Inferred from direct assay PubMed 18166528. Source: MGI

manganese ion binding

Inferred from electronic annotation. Source: Ensembl

nickel cation binding

Inferred from electronic annotation. Source: Ensembl

potassium ion binding

Inferred from electronic annotation. Source: Ensembl

sodium ion binding

Inferred from electronic annotation. Source: Ensembl

symporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UI40-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UI40-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-376: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661Sodium/potassium/calcium exchanger 2
PRO_0000019367

Regions

Topological domain1 – 3838Cytoplasmic Potential
Transmembrane39 – 5921Helical; Potential
Topological domain60 – 13273Extracellular Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 17825Cytoplasmic Potential
Transmembrane179 – 19921Helical; Potential
Topological domain200 – 2045Extracellular Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 24318Cytoplasmic Potential
Transmembrane244 – 26421Helical; Potential
Topological domain2651Extracellular Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 497211Cytoplasmic Potential
Transmembrane498 – 51821Helical; Potential
Topological domain519 – 53315Extracellular Potential
Transmembrane534 – 55421Helical; Potential
Topological domain555 – 56915Cytoplasmic Potential
Transmembrane570 – 59021Helical; Potential
Topological domain591 – 60212Extracellular Potential
Transmembrane603 – 62321Helical; Potential
Topological domain624 – 6307Cytoplasmic Potential
Transmembrane631 – 65121Helical; Potential
Topological domain652 – 66110Extracellular Potential
Repeat174 – 21441Alpha-1
Repeat541 – 57232Alpha-2

Amino acid modifications

Glycosylation1111N-linked (GlcNAc...) Ref.4

Natural variations

Alternative sequence360 – 37617Missing in isoform 2.
VSP_006164

Experimental info

Mutagenesis1111N → D: Loss of N-glycosylation. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: E6359C1F95C3AB3E

FASTA66173,664
        10         20         30         40         50         60 
MDLQQSTTIT SLEKWCLDES LSGCRRHYSV KKKLKLIRVL GLFMGLVAIS TVSFSISAFS 

        70         80         90        100        110        120 
ETDTQSTGEA SVVSGPRVAQ GYHQRTLLDL NDKILDYTPQ PPLSKEGESE NSTDHAQGDY 

       130        140        150        160        170        180 
PKDIFSLEER RKGAIILHVI GMIYMFIALA IVCDEFFVPS LTVITEKLGI SDDVAGATFM 

       190        200        210        220        230        240 
AAGGSAPELF TSLIGVFIAH SNVGIGTIVG SAVFNILFVI GMCALFSREI LNLTWWPLFR 

       250        260        270        280        290        300 
DVSFYIVDLI MLIIFFLDNV IMWWESLLLL TAYFCYVVFM KFNVQVEKWV KQMINRNKVV 

       310        320        330        340        350        360 
KVTAPEAQAK PSAARDKDEP TLPAKPRLQR GGSSASLHNS LMRNSIFQLM IHTLDPLAEE 

       370        380        390        400        410        420 
LGSYGKLKYY DTMTEEGRFR EKASILHKIA KKKCHVDENE RQNGAANHVE KIELPNSTST 

       430        440        450        460        470        480 
DVEMTPSSDA SEPVQNGNLS HNIEGAEAQT ADEEEDQPLS LAWPSETRKQ VTFLIVFPIV 

       490        500        510        520        530        540 
FPLWITLPDV RKPSSRKFFP ITFFGSITWI AVFSYLMVWW AHQVGETIGI SEEIMGLTIL 

       550        560        570        580        590        600 
AAGTSIPDLI TSVIVARKGL GDMAVSSSVG SNIFDITVGL PLPWLLYTVI HRFQPVAVSS 

       610        620        630        640        650        660 
NGLFCAIVLL FIMLLFVILS IALCKWRMNK ILGFIMFGLY FVFLVVSVLL EDRILTCPVS 


I 

« Hide

Isoform 2 [UniParc].

Checksum: 1DFB85DFA82A3F4B
Show »

FASTA64471,655

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression of the potassium-dependent sodium-calcium exchanger from human and chicken retinal cone photoreceptors."
Prinsen C.F.M., Szerencsei R.T., Schnetkamp P.P.M.
J. Neurosci. 20:1424-1434(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Retina.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[4]"Topology of the retinal cone NCKX2 Na/Ca-K exchanger."
Kinjo T.G., Szerencsei R.T., Winkfein R.J., Kang K., Schnetkamp P.P.
Biochemistry 42:2485-2491(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY, MUTAGENESIS OF ASN-111, GLYCOSYLATION AT ASN-111.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF097366 mRNA. Translation: AAF21810.1.
AF177987 mRNA. Translation: AAF25811.1.
AL158077, AL133281, AL158206 Genomic DNA. Translation: CAH74108.1.
AL158206, AL133281, AL158077 Genomic DNA. Translation: CAH73020.1.
AL133281, AL158077, AL158206 Genomic DNA. Translation: CAH71736.1.
AL158077, AL133281, AL158206 Genomic DNA. Translation: CAH74109.1.
AL158206, AL133281, AL158077 Genomic DNA. Translation: CAH73021.1.
AL133281, AL158077, AL158206 Genomic DNA. Translation: CAH71737.1.
BC069622 mRNA. Translation: AAH69622.1.
BC143889 mRNA. Translation: AAI43890.1.
RefSeqNP_001180217.1. NM_001193288.2.
NP_065077.1. NM_020344.3.
XP_005251482.1. XM_005251425.1.
XP_005251483.1. XM_005251426.2.
UniGeneHs.283014.
Hs.590750.
Hs.738749.

3D structure databases

ProteinModelPortalQ9UI40.
SMRQ9UI40. Positions 155-277, 471-576.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.19.4.11. the ca(2+):cation antiporter (caca) family.

PTM databases

PhosphoSiteQ9UI40.

Polymorphism databases

DMDM17865516.

Proteomic databases

PaxDbQ9UI40.
PRIDEQ9UI40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000286344; ENSP00000286344; ENSG00000155886. [Q9UI40-2]
ENST00000341998; ENSP00000344801; ENSG00000155886. [Q9UI40-1]
GeneID25769.
KEGGhsa:25769.
UCSCuc003zoa.2. human. [Q9UI40-1]
uc003zob.2. human. [Q9UI40-2]

Organism-specific databases

CTD25769.
GeneCardsGC09M019507.
HGNCHGNC:10976. SLC24A2.
HPAHPA057278.
MIM609838. gene.
neXtProtNX_Q9UI40.
PharmGKBPA35852.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0530.
HOGENOMHOG000231933.
HOVERGENHBG054881.
InParanoidQ9UI40.
KOK13750.
OMAGYHQRTL.
OrthoDBEOG776SP8.
PhylomeDBQ9UI40.
TreeFamTF318759.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

BgeeQ9UI40.
CleanExHS_SLC24A2.
GenevestigatorQ9UI40.

Family and domain databases

InterProIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
[Graphical view]
PANTHERPTHR10846. PTHR10846. 1 hit.
PfamPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsTIGR00367. TIGR00367. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC24A2. human.
GenomeRNAi25769.
NextBio46893.
PROQ9UI40.
SOURCESearch...

Entry information

Entry nameNCKX2_HUMAN
AccessionPrimary (citable) accession number: Q9UI40
Secondary accession number(s): B7ZLL8, Q9NTN5, Q9NZQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM