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Protein

Probable threonine protease PRSS50

Gene

PRSS50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in proteolysis through its threonine endopeptidase activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531Charge relay systemPROSITE-ProRule annotation
Active sitei206 – 2061Charge relay systemPROSITE-ProRule annotation
Active sitei310 – 3101Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • threonine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease homolog

Protein family/group databases

MEROPSiS01.993.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable threonine protease PRSS50 (EC:3.4.25.-)
Alternative name(s):
Cancer/testis antigen 20
Serine protease 50
Testis-specific protease-like protein 50
Gene namesi
Name:PRSS50
Synonyms:CT20, TSP50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17910. PRSS50.

Subcellular locationi

  • Endoplasmic reticulum 1 Publication

  • Note: May also localize to cytoplasmic membranes.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi310 – 3101T → A: Loss of catalytic activity. 1 Publication

Organism-specific databases

PharmGKBiPA165698443.

Polymorphism and mutation databases

BioMutaiPRSS50.
DMDMi37089988.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939Sequence analysisAdd
BLAST
Chaini40 – 385346Probable threonine protease PRSS50PRO_0000028492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi138 ↔ 154PROSITE-ProRule annotation
Disulfide bondi240 ↔ 316PROSITE-ProRule annotation
Disulfide bondi273 ↔ 296PROSITE-ProRule annotation
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis
Disulfide bondi306 ↔ 334PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9UI38.
PeptideAtlasiQ9UI38.
PRIDEiQ9UI38.

PTM databases

iPTMnetiQ9UI38.
PhosphoSiteiQ9UI38.

Expressioni

Tissue specificityi

Testis specific. Differentially expressed in some breast cancer tissues.

Gene expression databases

BgeeiQ9UI38.
GenevisibleiQ9UI38. HS.

Organism-specific databases

HPAiCAB008977.
HPA040768.

Interactioni

Protein-protein interaction databases

BioGridi118887. 24 interactions.
IntActiQ9UI38. 6 interactions.
STRINGi9606.ENSP00000326598.

Structurei

3D structure databases

ProteinModelPortaliQ9UI38.
SMRiQ9UI38. Positions 65-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 358266Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000065742.
HOVERGENiHBG068181.
InParanoidiQ9UI38.
OMAiMWPQFRT.
OrthoDBiEOG7RJPRS.
PhylomeDBiQ9UI38.
TreeFamiTF351676.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UI38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRWCQTVAR GQRPRTSAPS RAGALLLLLL LLRSAGCWGA GEAPGALSTA
60 70 80 90 100
DPADQSVQCV PKATCPSSRP RLLWQTPTTQ TLPSTTMETQ FPVSEGKVDP
110 120 130 140 150
YRSCGFSYEQ DPTLRDPEAV ARRWPWMVSV RANGTHICAG TIIASQWVLT
160 170 180 190 200
VAHCLIWRDV IYSVRVGSPW IDQMTQTASD VPVLQVIMHS RYRAQRFWSW
210 220 230 240 250
VGQANDIGLL KLKQELKYSN YVRPICLPGT DYVLKDHSRC TVTGWGLSKA
260 270 280 290 300
DGMWPQFRTI QEKEVIILNN KECDNFYHNF TKIPTLVQII KSQMMCAEDT
310 320 330 340 350
HREKFCYELT GEPLVCSMEG TWYLVGLVSW GAGCQKSEAP PIYLQVSSYQ
360 370 380
HWIWDCLNGQ ALALPAPSRT LLLALPLPLS LLAAL
Length:385
Mass (Da):43,088
Last modified:May 1, 2000 - v1
Checksum:iF5B39D6E12798AE5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751Q → P.
Corresponds to variant rs34788938 [ dbSNP | Ensembl ].
VAR_051859
Natural varianti98 – 981V → I.
Corresponds to variant rs35866901 [ dbSNP | Ensembl ].
VAR_051860

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100707 mRNA. Translation: AAF22500.1.
BC033016 mRNA. Translation: AAH33016.1.
BC037775 mRNA. Translation: AAH37775.1.
CCDSiCCDS2745.1.
RefSeqiNP_037402.1. NM_013270.4.
UniGeneiHs.120365.

Genome annotation databases

EnsembliENST00000315170; ENSP00000326598; ENSG00000206549.
ENST00000460241; ENSP00000418875; ENSG00000206549.
GeneIDi29122.
KEGGihsa:29122.
UCSCiuc003cqe.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100707 mRNA. Translation: AAF22500.1.
BC033016 mRNA. Translation: AAH33016.1.
BC037775 mRNA. Translation: AAH37775.1.
CCDSiCCDS2745.1.
RefSeqiNP_037402.1. NM_013270.4.
UniGeneiHs.120365.

3D structure databases

ProteinModelPortaliQ9UI38.
SMRiQ9UI38. Positions 65-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118887. 24 interactions.
IntActiQ9UI38. 6 interactions.
STRINGi9606.ENSP00000326598.

Protein family/group databases

MEROPSiS01.993.

PTM databases

iPTMnetiQ9UI38.
PhosphoSiteiQ9UI38.

Polymorphism and mutation databases

BioMutaiPRSS50.
DMDMi37089988.

Proteomic databases

PaxDbiQ9UI38.
PeptideAtlasiQ9UI38.
PRIDEiQ9UI38.

Protocols and materials databases

DNASUi29122.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315170; ENSP00000326598; ENSG00000206549.
ENST00000460241; ENSP00000418875; ENSG00000206549.
GeneIDi29122.
KEGGihsa:29122.
UCSCiuc003cqe.2. human.

Organism-specific databases

CTDi29122.
GeneCardsiPRSS50.
HGNCiHGNC:17910. PRSS50.
HPAiCAB008977.
HPA040768.
MIMi607950. gene.
neXtProtiNX_Q9UI38.
PharmGKBiPA165698443.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOGENOMiHOG000065742.
HOVERGENiHBG068181.
InParanoidiQ9UI38.
OMAiMWPQFRT.
OrthoDBiEOG7RJPRS.
PhylomeDBiQ9UI38.
TreeFamiTF351676.

Miscellaneous databases

GenomeRNAii29122.
PROiQ9UI38.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UI38.
GenevisibleiQ9UI38. HS.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a novel gene, TSP50, by a hypomethylated DNA fragment in human breast cancer."
    Yuan L., Shan J., De Risi D., Broome J., Lovecchio J., Gal D., Vinciguerra V., Xu H.-P.
    Cancer Res. 59:3215-3221(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary cancer.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "TSP50 encodes a testis-specific protease and is negatively regulated by p53."
    Xu H., Shan J., Jurukovski V., Yuan L., Li J., Tian K.
    Cancer Res. 67:1239-1245(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-310.

Entry informationi

Entry nameiTSP50_HUMAN
AccessioniPrimary (citable) accession number: Q9UI38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

DNA hypomethylation is accompanied by the expression of the gene in the testis.

Caution

Although related to peptidase S1 family, lacks the conserved active Ser residue in position 310 which is replaced by a Thr.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.