Q9UI14 (PRAF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prenylated Rab acceptor protein 1

Alternative name(s):
PRA1 family protein 1

Gene names

Name:	RABAC1
Synonyms:	PRA1, PRAF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI By similarity.
Subunit structure	Homodimer. Interacts with VAMP2 (synaptobrevin-2), GDI1, and PCLO By similarity. Interacts specifically with prenylated Rab proteins; strongly with RAB4B, RAB5A and RAB5C, and weakly with RAB4A, RAB6, RAB7A, RAB17 and RAB22. Interacts with NDRG1. Ref.1 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Cytoplasm By similarity. Golgi apparatus By similarity. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle By similarity. Note: According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles By similarity. Ref.1
Tissue specificity	Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach. Ref.7
Developmental stage	In fetal tissues, it is more abundant in kidney and lung. Ref.7
Sequence similarities	Belongs to the PRA1 family.
Caution	In contrast to the mouse ortholog, it does not interact with Ras.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane Synapse
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-712367,EBI-712367
FXR2	P51116	3	EBI-712367,EBI-740459
NIF3L1	Q9GZT8	3	EBI-712367,EBI-740897
NUDT18	Q6ZVK8	3	EBI-712367,EBI-740486
PLEKHF2	Q9H8W4	3	EBI-712367,EBI-742388
RAB33A	Q14088	3	EBI-712367,EBI-744685
SMCR7	Q96C03	3	EBI-712367,EBI-750153
SNCA	P37840	4	EBI-712367,EBI-985879
WDYHV1	Q96HA8	3	EBI-712367,EBI-741158

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Prenylated Rab acceptor protein 1		PRO_0000220878
Regions
	Topological domain	1 – 78	78	Cytoplasmic By similarity
	Transmembrane	79 – 94	16	Helical; By similarity
	Transmembrane	95 – 112	18	Helical; By similarity
	Topological domain	113 – 131	19	Cytoplasmic By similarity
	Transmembrane	132 – 148	17	Helical; By similarity
	Transmembrane	149 – 165	17	Helical; By similarity
	Topological domain	166 – 185	20	Cytoplasmic By similarity
	Region	30 – 54	25	Required for interaction with prenylated RAB3A and VAMP2 By similarity
	Region	165 – 185	21	Required for interaction with GDI1 By similarity
	Region	175 – 185	11	Homodimerization By similarity
	Region	175 – 185	11	Required for interaction with prenylated RAB3A and VAMP2 By similarity
Experimental info
	Sequence conflict	2	1	Missing in AAP97229. Ref.3
	Sequence conflict	40 – 41	2	AT → GP in AAP97229. Ref.3
	Sequence conflict	129	1	A → E in CAB43107. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9UI14 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A85BD5BDCF6C23E4	FASTA	185	20,648
	10 20 30 40 50 60 MAAQKDQQKD AEAEGLSGTT LLPKLIPSGA GREWLERRRA TIRPWSTFVD QQRFSRPRNL 70 80 90 100 110 120 GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL AVFFGACYIL YLRTLESKLV 130 140 150 160 170 180 LFGREVSPAH QYALAGGISF PFFWLAGAGS AVFWVLGATL VVIGSHAAFH QIEAVDGEEL QMEPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)." Bucci C., Chiariello M., Lattero D., Maiorano M., Bruni C.B. Biochem. Biophys. Res. Commun. 258:657-662(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB GTPASES, SUBCELLULAR LOCATION. Tissue: Brain and Placenta.
[2]	"A novel gene expressed in human hypothalamus." Jin W., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Jiang C., Li Y., Han Z., Wang Y., Chen Z., Fu G. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hypothalamus.
[3]	"Cloning and characterization of a new human cDNA homologous to Rattus norvegicus prenylated rab acceptor 1 (PRA1) mRNA." Fu Q., Yu L., Yue P., Dai F.Y., Wang X.K., Zhao S.Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[7]	"Expression analysis and chromosomal assignment of PRA1 and RILP genes." Bucci C., De Gregorio L., Bruni C.B. Biochem. Biophys. Res. Commun. 286:815-819(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]	"NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24." Hunter M., Angelicheva D., Tournev I., Ingley E., Chan D.C., Watts G.F., Kremensky I., Kalaydjieva L. Biochem. Biophys. Res. Commun. 332:982-992(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NDRG1.
[9]	"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)." Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S. Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ133534 mRNA. Translation: CAB43107.1. AF112202 mRNA. Translation: AAF17190.1. AF112996 mRNA. Translation: AAP97229.1. CR457214 mRNA. Translation: CAG33495.1. CR542116 mRNA. Translation: CAG46913.1. BT019964 mRNA. Translation: AAV38767.1. BC008950 mRNA. Translation: AAH08950.1.
IPI	IPI00007909.
RefSeq	NP_006414.2. NM_006423.2.
UniGene	Hs.11417.
3D structure databases
ProteinModelPortal	Q9UI14.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UI14. 32 interactions.
MINT	MINT-199369.
STRING	9606.ENSP00000222008.
PTM databases
PhosphoSite	Q9UI14.
Polymorphism databases
DMDM	56404978.
Proteomic databases
PaxDb	Q9UI14.
PRIDE	Q9UI14.
Protocols and materials databases
DNASU	10567.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000222008; ENSP00000222008; ENSG00000105404.
GeneID	10567.
KEGG	hsa:10567.
UCSC	uc002osf.3. human.
Organism-specific databases
CTD	10567.
GeneCards	GC19M042460.
HGNC	HGNC:9794. RABAC1.
HPA	CAB019320. HPA029171.
MIM	604925. gene.
neXtProt	NX_Q9UI14.
PharmGKB	PA34155.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG129644.
HOGENOM	HOG000230621.
HOVERGEN	HBG053660.
InParanoid	Q9UI14.
OMA	LGASCFV.
OrthoDB	EOG4NCMDT.
PhylomeDB	Q9UI14.
Gene expression databases
Bgee	Q9UI14.
CleanEx	HS_RABAC1.
Genevestigator	Q9UI14.
GermOnline	ENSG00000105404. Homo sapiens.
Family and domain databases
InterPro	IPR004895. Prenylated_rab_accept_PRA1. [Graphical view]
Pfam	PF03208. PRA1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10567.
NextBio	40105.
SOURCE	Search...

Entry information

Entry name

PRAF1_HUMAN

Accession

Primary (citable) accession number: Q9UI14
Secondary accession number(s): Q7Z4Y2, Q9Y3R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.