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Protein

Prenylated Rab acceptor protein 1

Gene

RABAC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI.By similarity

GO - Molecular functioni

  • identical protein binding Source: IntAct
Complete GO annotation...

Protein family/group databases

TCDBi9.A.49.1.1. the preylated rab acceptor protein 1 (pra1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Prenylated Rab acceptor protein 1
Alternative name(s):
PRA1 family protein 1
Gene namesi
Name:RABAC1
Synonyms:PRA1, PRAF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9794. RABAC1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cytoplasm By similarity
  • Golgi apparatus By similarity
  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle By similarity

  • Note: According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878CytoplasmicBy similarityAdd
BLAST
Transmembranei79 – 9416HelicalBy similarityAdd
BLAST
Transmembranei95 – 11218HelicalBy similarityAdd
BLAST
Topological domaini113 – 13119CytoplasmicBy similarityAdd
BLAST
Transmembranei132 – 14817HelicalBy similarityAdd
BLAST
Transmembranei149 – 16517HelicalBy similarityAdd
BLAST
Topological domaini166 – 18520CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • Golgi apparatus Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • synaptic vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34155.

Polymorphism and mutation databases

BioMutaiRABAC1.
DMDMi56404978.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Prenylated Rab acceptor protein 1PRO_0000220878Add
BLAST

Proteomic databases

EPDiQ9UI14.
MaxQBiQ9UI14.
PaxDbiQ9UI14.
PRIDEiQ9UI14.
TopDownProteomicsiQ9UI14.

PTM databases

iPTMnetiQ9UI14.
PhosphoSiteiQ9UI14.

Expressioni

Tissue specificityi

Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach.1 Publication

Developmental stagei

In fetal tissues, it is more abundant in kidney and lung.1 Publication

Gene expression databases

BgeeiQ9UI14.
CleanExiHS_RABAC1.
ExpressionAtlasiQ9UI14. baseline and differential.
GenevisibleiQ9UI14. HS.

Organism-specific databases

HPAiCAB019320.
HPA029171.

Interactioni

Subunit structurei

Homodimer. Interacts with VAMP2 (synaptobrevin-2), GDI1, and PCLO (By similarity). Interacts specifically with prenylated Rab proteins; strongly with RAB4B, RAB5A and RAB5C, and weakly with RAB4A, RAB6, RAB7A, RAB17 and RAB22. Interacts with NDRG1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-712367,EBI-712367
ADCK3Q8NI606EBI-712367,EBI-745535
DUSP12Q9UNI64EBI-712367,EBI-715161
FXR2P511163EBI-712367,EBI-740459
LMP1P032309EBI-712367,EBI-6973030From a different organism.
MIEF2Q96C033EBI-712367,EBI-750153
NDRG2Q9UN36-17EBI-712367,EBI-8084503
NDRG4Q9ULP03EBI-712367,EBI-10323810
NIF3L1Q9GZT83EBI-712367,EBI-740897
NUDT18Q6ZVK83EBI-712367,EBI-740486
PLEKHF2Q9H8W43EBI-712367,EBI-742388
RAB33AQ140883EBI-712367,EBI-744685
RIC8AQ9NPQ8-43EBI-712367,EBI-9091816
SNCAP378404EBI-712367,EBI-985879
SNX1Q135963EBI-712367,EBI-2822329
SNX10Q8N5Z33EBI-712367,EBI-10266928
SNX11Q9Y5W93EBI-712367,EBI-10329449
SNX15Q9NRS64EBI-712367,EBI-725924
SPG21Q9NZD84EBI-712367,EBI-742688
SYT16Q17RD73EBI-712367,EBI-10238936
TRIM32Q130493EBI-712367,EBI-742790
WDYHV1Q96HA86EBI-712367,EBI-741158
WIPI2Q9Y4P83EBI-712367,EBI-719396
ZFYVE21Q9BQ243EBI-712367,EBI-2849569

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi115818. 55 interactions.
IntActiQ9UI14. 52 interactions.
MINTiMINT-199369.
STRINGi9606.ENSP00000222008.

Structurei

3D structure databases

ProteinModelPortaliQ9UI14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 5425Required for interaction with prenylated RAB3A and VAMP2By similarityAdd
BLAST
Regioni165 – 18521Required for interaction with GDI1By similarityAdd
BLAST
Regioni175 – 18511HomodimerizationBy similarityAdd
BLAST
Regioni175 – 18511Required for interaction with prenylated RAB3A and VAMP2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiQ9UI14.
OMAiVVFIGWI.
PhylomeDBiQ9UI14.
TreeFamiTF324857.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UI14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQKDQQKD AEAEGLSGTT LLPKLIPSGA GREWLERRRA TIRPWSTFVD
60 70 80 90 100
QQRFSRPRNL GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL
110 120 130 140 150
AVFFGACYIL YLRTLESKLV LFGREVSPAH QYALAGGISF PFFWLAGAGS
160 170 180
AVFWVLGATL VVIGSHAAFH QIEAVDGEEL QMEPV
Length:185
Mass (Da):20,648
Last modified:May 1, 2000 - v1
Checksum:iA85BD5BDCF6C23E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21Missing in AAP97229 (Ref. 3) Curated
Sequence conflicti40 – 412AT → GP in AAP97229 (Ref. 3) Curated
Sequence conflicti129 – 1291A → E in CAB43107 (PubMed:10329441).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133534 mRNA. Translation: CAB43107.1.
AF112202 mRNA. Translation: AAF17190.1.
AF112996 mRNA. Translation: AAP97229.1.
CR457214 mRNA. Translation: CAG33495.1.
CR542116 mRNA. Translation: CAG46913.1.
BT019964 mRNA. Translation: AAV38767.1.
BC008950 mRNA. Translation: AAH08950.1.
CCDSiCCDS12593.1.
RefSeqiNP_006414.2. NM_006423.2.
UniGeneiHs.11417.

Genome annotation databases

EnsembliENST00000222008; ENSP00000222008; ENSG00000105404.
GeneIDi10567.
KEGGihsa:10567.
UCSCiuc002osf.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133534 mRNA. Translation: CAB43107.1.
AF112202 mRNA. Translation: AAF17190.1.
AF112996 mRNA. Translation: AAP97229.1.
CR457214 mRNA. Translation: CAG33495.1.
CR542116 mRNA. Translation: CAG46913.1.
BT019964 mRNA. Translation: AAV38767.1.
BC008950 mRNA. Translation: AAH08950.1.
CCDSiCCDS12593.1.
RefSeqiNP_006414.2. NM_006423.2.
UniGeneiHs.11417.

3D structure databases

ProteinModelPortaliQ9UI14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115818. 55 interactions.
IntActiQ9UI14. 52 interactions.
MINTiMINT-199369.
STRINGi9606.ENSP00000222008.

Protein family/group databases

TCDBi9.A.49.1.1. the preylated rab acceptor protein 1 (pra1) family.

PTM databases

iPTMnetiQ9UI14.
PhosphoSiteiQ9UI14.

Polymorphism and mutation databases

BioMutaiRABAC1.
DMDMi56404978.

Proteomic databases

EPDiQ9UI14.
MaxQBiQ9UI14.
PaxDbiQ9UI14.
PRIDEiQ9UI14.
TopDownProteomicsiQ9UI14.

Protocols and materials databases

DNASUi10567.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222008; ENSP00000222008; ENSG00000105404.
GeneIDi10567.
KEGGihsa:10567.
UCSCiuc002osf.4. human.

Organism-specific databases

CTDi10567.
GeneCardsiRABAC1.
HGNCiHGNC:9794. RABAC1.
HPAiCAB019320.
HPA029171.
MIMi604925. gene.
neXtProtiNX_Q9UI14.
PharmGKBiPA34155.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiQ9UI14.
OMAiVVFIGWI.
PhylomeDBiQ9UI14.
TreeFamiTF324857.

Miscellaneous databases

GeneWikiiRABAC1.
GenomeRNAii10567.
PROiQ9UI14.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UI14.
CleanExiHS_RABAC1.
ExpressionAtlasiQ9UI14. baseline and differential.
GenevisibleiQ9UI14. HS.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)."
    Bucci C., Chiariello M., Lattero D., Maiorano M., Bruni C.B.
    Biochem. Biophys. Res. Commun. 258:657-662(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB GTPASES, SUBCELLULAR LOCATION.
    Tissue: Brain and Placenta.
  2. "A novel gene expressed in human hypothalamus."
    Jin W., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Jiang C., Li Y., Han Z., Wang Y., Chen Z., Fu G.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Hypothalamus.
  3. "Cloning and characterization of a new human cDNA homologous to Rattus norvegicus prenylated rab acceptor 1 (PRA1) mRNA."
    Fu Q., Yu L., Yue P., Dai F.Y., Wang X.K., Zhao S.Y.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  7. "Expression analysis and chromosomal assignment of PRA1 and RILP genes."
    Bucci C., De Gregorio L., Bruni C.B.
    Biochem. Biophys. Res. Commun. 286:815-819(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24."
    Hunter M., Angelicheva D., Tournev I., Ingley E., Chan D.C., Watts G.F., Kremensky I., Kalaydjieva L.
    Biochem. Biophys. Res. Commun. 332:982-992(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDRG1.
  9. "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
    Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
    Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRAF1_HUMAN
AccessioniPrimary (citable) accession number: Q9UI14
Secondary accession number(s): Q7Z4Y2, Q9Y3R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

In contrast to the mouse ortholog, it does not interact with Ras.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.