Reviewed,
UniProtKB/Swiss-Prot Q9UI14 (PRAF1_HUMAN)
Last modified
June 16, 2009.
Version 55.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Prenylated Rab acceptor protein 1 Alternative name(s): PRA1 family protein 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 185 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI By similarity. |
| Subunit structure | Homodimer. Interacts with VAMP2 (synaptobrevin-2), GDI1, and PCLO By similarity. Interacts specifically with prenylated Rab proteins; strongly with RAB4B, RAB5A and RAB5C, and weakly with RAB4A, RAB6, RAB7, RAB17 and RAB22. |
| Subcellular location | Cell membrane; Multi-pass membrane protein By similarity. Cytoplasm By similarity. Golgi apparatus By similarity. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle By similarity. Note: According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles By similarity. |
| Tissue specificity | Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach. Ref.7 |
| Developmental stage | In fetal tissues, it is more abundant in kidney and lung. Ref.7 |
| Sequence similarities | Belongs to the PRA1 family. |
| Caution | In contrast to the mouse ortholog, it does not interact with Ras. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane Synapse |
| Domain | Transmembrane |
| Gene Ontology (GO) | |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell junctionInferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-KW secretory granuleInferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicleInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | identical protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 185 | 185 | Prenylated Rab acceptor protein 1 | PRO_0000220878 | |||||
Regions | |||||||||
| Topological domain | 1 – 78 | 78 | Cytoplasmic By similarity | ||||||
| Transmembrane | 79 – 94 | 16 | By similarity | ||||||
| Transmembrane | 95 – 112 | 18 | By similarity | ||||||
| Topological domain | 113 – 131 | 19 | Cytoplasmic By similarity | ||||||
| Transmembrane | 132 – 148 | 17 | By similarity | ||||||
| Transmembrane | 149 – 165 | 17 | By similarity | ||||||
| Topological domain | 166 – 185 | 20 | Cytoplasmic By similarity | ||||||
| Region | 30 – 54 | 25 | Required for interaction with prenylated RAB3A and VAMP2 By similarity | ||||||
| Region | 165 – 185 | 21 | Required for interaction with GDI1 By similarity | ||||||
| Region | 175 – 185 | 11 | Homodimerization By similarity | ||||||
| Region | 175 – 185 | 11 | Required for interaction with prenylated RAB3A and VAMP2 By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 2 | 1 | Missing Ref.3 | ||||||
| Sequence conflict | 40 – 41 | 2 | AT → GP in AAP97229. Ref.3 | ||||||
| Sequence conflict | 129 | 1 | A → E in CAB43107. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)." Bucci C., Chiariello M., Lattero D., Maiorano M., Bruni C.B. Biochem. Biophys. Res. Commun. 258:657-662(1999) [PubMed: 10329441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB GTPASES, SUBCELLULAR LOCATION. Tissue: Brain and Placenta. |
| [2] | "A novel gene expressed in human hypothalamus." Jin W., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Jiang C., Li Y., Han Z., Wang Y., Chen Z., Fu G. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hypothalamus. |
| [3] | "Cloning and characterization of a new human cDNA homologous to Rattus norvegicus prenylated rab acceptor 1 (PRA1) mRNA." Fu Q., Yu L., Yue P., Dai F.Y., Wang X.K., Zhao S.Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
| [7] | "Expression analysis and chromosomal assignment of PRA1 and RILP genes." Bucci C., De Gregorio L., Bruni C.B. Biochem. Biophys. Res. Commun. 286:815-819(2001) [PubMed: 11520070] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| [8] | "Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)." Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S. Gene 371:154-165(2006) [PubMed: 16481131] [Abstract] Cited for: NOMENCLATURE. |
| [9] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AJ133534 mRNA. Translation: CAB43107.1. AF112202 mRNA. Translation: AAF17190.1. AF112996 mRNA. Translation: AAP97229.1. CR457214 mRNA. Translation: CAG33495.1. CR542116 mRNA. Translation: CAG46913.1. BT019964 mRNA. Translation: AAV38767.1. BC008950 mRNA. Translation: AAH08950.1. | |
| IPI | IPI00007909. |
| RefSeq | NP_006414.2. |
| UniGene | Hs.11417 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9UI14. 18 interactions. |
Proteomic databases | |
| PRIDE | Q9UI14. |
Genome annotation databases | |
| Ensembl | ENSG00000105404. Homo sapiens. [Contig view] |
| GeneID | 10567. |
| KEGG | hsa:10567. |
Organism-specific databases | |
| GeneCards | GC19M047152. |
| H-InvDB | HIX0015164. |
| HGNC | HGNC:9794. RABAC1. |
| HPA | CAB019320. |
| MIM | 604925. gene. |
| PharmGKB | PA34155. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9UI14. |
| HOVERGEN | Q9UI14. |
| OMA | Q9UI14. AGGISFP. |
Gene expression databases | |
| ArrayExpress | Q9UI14. |
| Bgee | Q9UI14. |
| CleanEx | HS_RABAC1. |
| GermOnline | ENSG00000105404. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR004895. Prenylated_rab_accept_PRA1. [Graphical view] |
| Pfam | PF03208. PRA1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 40105. |
| SOURCE | Search... |
Entry information
| Entry name | PRAF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9UI14 Secondary accession number(s): Q7Z4Y2, Q9Y3R1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


