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Q9UI14 (PRAF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prenylated Rab acceptor protein 1
Alternative name(s):
PRA1 family protein 1
Gene names
Name:RABAC1
Synonyms:PRA1, PRAF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI By similarity.

Subunit structure

Homodimer. Interacts with VAMP2 (synaptobrevin-2), GDI1, and PCLO By similarity. Interacts specifically with prenylated Rab proteins; strongly with RAB4B, RAB5A and RAB5C, and weakly with RAB4A, RAB6, RAB7A, RAB17 and RAB22. Interacts with NDRG1. Ref.1 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Cytoplasm By similarity. Golgi apparatus By similarity. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle By similarity. Note: According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles By similarity. Ref.1

Tissue specificity

Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach. Ref.7

Developmental stage

In fetal tissues, it is more abundant in kidney and lung. Ref.7

Sequence similarities

Belongs to the PRA1 family.

Caution

In contrast to the mouse ortholog, it does not interact with Ras.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Prenylated Rab acceptor protein 1
PRO_0000220878

Regions

Topological domain1 – 7878Cytoplasmic By similarity
Transmembrane79 – 9416Helical; By similarity
Transmembrane95 – 11218Helical; By similarity
Topological domain113 – 13119Cytoplasmic By similarity
Transmembrane132 – 14817Helical; By similarity
Transmembrane149 – 16517Helical; By similarity
Topological domain166 – 18520Cytoplasmic By similarity
Region30 – 5425Required for interaction with prenylated RAB3A and VAMP2 By similarity
Region165 – 18521Required for interaction with GDI1 By similarity
Region175 – 18511Homodimerization By similarity
Region175 – 18511Required for interaction with prenylated RAB3A and VAMP2 By similarity

Experimental info

Sequence conflict21Missing in AAP97229. Ref.3
Sequence conflict40 – 412AT → GP in AAP97229. Ref.3
Sequence conflict1291A → E in CAB43107. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9UI14 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: A85BD5BDCF6C23E4

FASTA18520,648
        10         20         30         40         50         60 
MAAQKDQQKD AEAEGLSGTT LLPKLIPSGA GREWLERRRA TIRPWSTFVD QQRFSRPRNL 

        70         80         90        100        110        120 
GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL AVFFGACYIL YLRTLESKLV 

       130        140        150        160        170        180 
LFGREVSPAH QYALAGGISF PFFWLAGAGS AVFWVLGATL VVIGSHAAFH QIEAVDGEEL 


QMEPV 

« Hide

References

« Hide 'large scale' references
[1]"Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)."
Bucci C., Chiariello M., Lattero D., Maiorano M., Bruni C.B.
Biochem. Biophys. Res. Commun. 258:657-662(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB GTPASES, SUBCELLULAR LOCATION.
Tissue: Brain and Placenta.
[2]"A novel gene expressed in human hypothalamus."
Jin W., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Jiang C., Li Y., Han Z., Wang Y., Chen Z., Fu G.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Hypothalamus.
[3]"Cloning and characterization of a new human cDNA homologous to Rattus norvegicus prenylated rab acceptor 1 (PRA1) mRNA."
Fu Q., Yu L., Yue P., Dai F.Y., Wang X.K., Zhao S.Y.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[7]"Expression analysis and chromosomal assignment of PRA1 and RILP genes."
Bucci C., De Gregorio L., Bruni C.B.
Biochem. Biophys. Res. Commun. 286:815-819(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]"NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24."
Hunter M., Angelicheva D., Tournev I., Ingley E., Chan D.C., Watts G.F., Kremensky I., Kalaydjieva L.
Biochem. Biophys. Res. Commun. 332:982-992(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDRG1.
[9]"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)."
Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S.
Gene 371:154-165(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ133534 mRNA. Translation: CAB43107.1.
AF112202 mRNA. Translation: AAF17190.1.
AF112996 mRNA. Translation: AAP97229.1.
CR457214 mRNA. Translation: CAG33495.1.
CR542116 mRNA. Translation: CAG46913.1.
BT019964 mRNA. Translation: AAV38767.1.
BC008950 mRNA. Translation: AAH08950.1.
IPIIPI00007909.
RefSeqNP_006414.2. NM_006423.2.
UniGeneHs.11417.

3D structure databases

ProteinModelPortalQ9UI14.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9UI14. 32 interactions.
MINTMINT-199369.
STRING9606.ENSP00000222008.

PTM databases

PhosphoSiteQ9UI14.

Polymorphism databases

DMDM56404978.

Proteomic databases

PaxDbQ9UI14.
PRIDEQ9UI14.

Protocols and materials databases

DNASU10567.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222008; ENSP00000222008; ENSG00000105404.
GeneID10567.
KEGGhsa:10567.
UCSCuc002osf.3. human.

Organism-specific databases

CTD10567.
GeneCardsGC19M042460.
HGNCHGNC:9794. RABAC1.
HPACAB019320.
HPA029171.
MIM604925. gene.
neXtProtNX_Q9UI14.
PharmGKBPA34155.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG129644.
HOGENOMHOG000230621.
HOVERGENHBG053660.
InParanoidQ9UI14.
OMALGASCFV.
OrthoDBEOG4NCMDT.
PhylomeDBQ9UI14.

Gene expression databases

BgeeQ9UI14.
CleanExHS_RABAC1.
GenevestigatorQ9UI14.
GermOnlineENSG00000105404. Homo sapiens.

Family and domain databases

InterProIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi10567.
NextBio40105.
SOURCESearch...

Entry information

Entry namePRAF1_HUMAN
AccessionPrimary (citable) accession number: Q9UI14
Secondary accession number(s): Q7Z4Y2, Q9Y3R1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 1, 2000
Last modified: May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families