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Protein

Enolase-phosphatase E1

Gene

ENOPH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).UniRule annotation1 Publication

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 and 4 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16MagnesiumUniRule annotation1 Publication1
Metal bindingi18Magnesium; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei187Substrate1
Metal bindingi212MagnesiumUniRule annotation1 Publication1

GO - Molecular functioni

  • acireductone synthase activity Source: UniProtKB
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS07242-MONOMER.
BRENDAi3.1.3.77. 2681.
ReactomeiR-HSA-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1UniRule annotation (EC:3.1.3.77UniRule annotation)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphataseUniRule annotation
MASA homologUniRule annotation
Gene namesi
Name:ENOPH1UniRule annotation
Synonyms:MASAUniRule annotation
ORF Names:MSTP145
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:24599. ENOPH1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi58478.
OpenTargetsiENSG00000145293.
PharmGKBiPA162385052.

Polymorphism and mutation databases

BioMutaiENOPH1.
DMDMi74735024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002540071 – 261Enolase-phosphatase E1Add BLAST261

Proteomic databases

EPDiQ9UHY7.
PaxDbiQ9UHY7.
PeptideAtlasiQ9UHY7.
PRIDEiQ9UHY7.

PTM databases

DEPODiQ9UHY7.
iPTMnetiQ9UHY7.
PhosphoSitePlusiQ9UHY7.

Expressioni

Gene expression databases

BgeeiENSG00000145293.
CleanExiHS_ENOPH1.
ExpressionAtlasiQ9UHY7. baseline and differential.
GenevisibleiQ9UHY7. HS.

Organism-specific databases

HPAiCAB004985.
HPA044607.
HPA050480.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF41Q9H4P44EBI-726969,EBI-2130266

Protein-protein interaction databases

BioGridi121811. 26 interactors.
IntActiQ9UHY7. 11 interactors.
MINTiMINT-1415620.
STRINGi9606.ENSP00000273920.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Turni19 – 21Combined sources3
Helixi24 – 29Combined sources6
Helixi31 – 46Combined sources16
Helixi50 – 65Combined sources16
Turni66 – 68Combined sources3
Helixi83 – 103Combined sources21
Helixi108 – 123Combined sources16
Helixi135 – 144Combined sources10
Beta strandi148 – 152Combined sources5
Helixi157 – 165Combined sources9
Helixi173 – 175Combined sources3
Beta strandi177 – 180Combined sources4
Helixi182 – 184Combined sources3
Helixi190 – 200Combined sources11
Helixi204 – 206Combined sources3
Beta strandi207 – 212Combined sources6
Helixi214 – 222Combined sources9
Beta strandi226 – 230Combined sources5
Helixi240 – 245Combined sources6
Beta strandi248 – 251Combined sources4
Helixi252 – 254Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YNSX-ray1.70A1-261[»]
1ZS9X-ray1.70A1-261[»]
ProteinModelPortaliQ9UHY7.
SMRiQ9UHY7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UHY7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 154Substrate binding2

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2630. Eukaryota.
COG4229. LUCA.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
HOVERGENiHBG054539.
InParanoidiQ9UHY7.
KOiK09880.
OMAiPYAKQNV.
OrthoDBiEOG091G0IX4.
PhylomeDBiQ9UHY7.
TreeFamiTF105939.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_01681. Salvage_MtnC. 1 hit.
MF_03117. Salvage_MtnC_euk. 1 hit.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UHY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE
60 70 80 90 100
ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ
110 120 130 140 150
MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY
160 170 180 190 200
IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI
210 220 230 240 250
GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT
260
SFSELYLPSS T
Length:261
Mass (Da):28,933
Last modified:May 1, 2000 - v1
Checksum:i12B3F73463907E2C
GO
Isoform 2 (identifier: Q9UHY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: Missing.

Show »
Length:115
Mass (Da):12,552
Checksum:i43E2FD7570657AFC
GO

Sequence cautioni

The sequence AAQ13671 differs from that shown. Reason: Frameshift at position 235.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211601 – 146Missing in isoform 2. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113125 mRNA. Translation: AAF14866.1.
AK022656 mRNA. Translation: BAB14160.1.
CR457141 mRNA. Translation: CAG33422.1.
BC001317 mRNA. Translation: AAH01317.1.
BC065815 mRNA. Translation: AAH65815.1.
AF177286 mRNA. Translation: AAQ13671.1. Sequence problems.
CCDSiCCDS3594.1. [Q9UHY7-1]
RefSeqiNP_001278946.1. NM_001292017.1.
NP_067027.1. NM_021204.4. [Q9UHY7-1]
UniGeneiHs.18442.

Genome annotation databases

EnsembliENST00000273920; ENSP00000273920; ENSG00000145293. [Q9UHY7-1]
ENST00000505846; ENSP00000427209; ENSG00000145293. [Q9UHY7-2]
GeneIDi58478.
KEGGihsa:58478.
UCSCiuc003hmv.4. human. [Q9UHY7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113125 mRNA. Translation: AAF14866.1.
AK022656 mRNA. Translation: BAB14160.1.
CR457141 mRNA. Translation: CAG33422.1.
BC001317 mRNA. Translation: AAH01317.1.
BC065815 mRNA. Translation: AAH65815.1.
AF177286 mRNA. Translation: AAQ13671.1. Sequence problems.
CCDSiCCDS3594.1. [Q9UHY7-1]
RefSeqiNP_001278946.1. NM_001292017.1.
NP_067027.1. NM_021204.4. [Q9UHY7-1]
UniGeneiHs.18442.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YNSX-ray1.70A1-261[»]
1ZS9X-ray1.70A1-261[»]
ProteinModelPortaliQ9UHY7.
SMRiQ9UHY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121811. 26 interactors.
IntActiQ9UHY7. 11 interactors.
MINTiMINT-1415620.
STRINGi9606.ENSP00000273920.

PTM databases

DEPODiQ9UHY7.
iPTMnetiQ9UHY7.
PhosphoSitePlusiQ9UHY7.

Polymorphism and mutation databases

BioMutaiENOPH1.
DMDMi74735024.

Proteomic databases

EPDiQ9UHY7.
PaxDbiQ9UHY7.
PeptideAtlasiQ9UHY7.
PRIDEiQ9UHY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273920; ENSP00000273920; ENSG00000145293. [Q9UHY7-1]
ENST00000505846; ENSP00000427209; ENSG00000145293. [Q9UHY7-2]
GeneIDi58478.
KEGGihsa:58478.
UCSCiuc003hmv.4. human. [Q9UHY7-1]

Organism-specific databases

CTDi58478.
DisGeNETi58478.
GeneCardsiENOPH1.
HGNCiHGNC:24599. ENOPH1.
HPAiCAB004985.
HPA044607.
HPA050480.
neXtProtiNX_Q9UHY7.
OpenTargetsiENSG00000145293.
PharmGKBiPA162385052.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2630. Eukaryota.
COG4229. LUCA.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
HOVERGENiHBG054539.
InParanoidiQ9UHY7.
KOiK09880.
OMAiPYAKQNV.
OrthoDBiEOG091G0IX4.
PhylomeDBiQ9UHY7.
TreeFamiTF105939.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.
BioCyciZFISH:HS07242-MONOMER.
BRENDAi3.1.3.77. 2681.
ReactomeiR-HSA-1237112. Methionine salvage pathway.

Miscellaneous databases

ChiTaRSiENOPH1. human.
EvolutionaryTraceiQ9UHY7.
GenomeRNAii58478.
PROiQ9UHY7.

Gene expression databases

BgeeiENSG00000145293.
CleanExiHS_ENOPH1.
ExpressionAtlasiQ9UHY7. baseline and differential.
GenevisibleiQ9UHY7. HS.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_01681. Salvage_MtnC. 1 hit.
MF_03117. Salvage_MtnC_euk. 1 hit.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
IPR006439. HAD-SF_hydro_IA.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.
TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENOPH_HUMAN
AccessioniPrimary (citable) accession number: Q9UHY7
Secondary accession number(s): Q7Z4C5, Q9BVC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.