Q9UHQ1 (NARF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear prelamin A recognition factor

Alternative name(s):
Iron-only hydrogenase-like protein 2
Short name=IOP2

Gene names

Name:

NARF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with LMNA and binds to the farnesylated C-terminal domain. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. Ref.1
Sequence similarities	Belongs to the NARF family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	lamin filament Inferred from direct assay Ref.1. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UHQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: S → SEISQAWWCTPVITATREAAARESLEPGRQRLQRDKIAPLDSSLGGG

Isoform 3 (identifier: Q9UHQ1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     38-85: Missing.

Isoform 4 (identifier: Q9UHQ1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Nuclear prelamin A recognition factor		PRO_0000288479
Natural variations
	Alternative sequence	1 – 59	59	Missing in isoform 4.		VSP_046371
	Alternative sequence	38 – 85	48	Missing in isoform 3.		VSP_025691
	Alternative sequence	256	1	S → SEISQAWWCTPVITATREAA ARESLEPGRQRLQRDKIAPL DSSLGGG in isoform 2.		VSP_025690
Experimental info
	Sequence conflict	154	1	R → L in BAG51834. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F615D812E7FC7D0F	FASTA	456	51,156
	10 20 30 40 50 60 MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL SDCLACDSCM 70 80 90 100 110 120 TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP QSLPYFAAKF NLSVTDASRR 130 140 150 160 170 180 LCGFLKSLGV HYVFDTTIAA DFSILESQKE FVRRYRQHSE EERTLPMLTS ACPGWVRYAE 190 200 210 220 230 240 RVLGRPITAH LCTAKSPQQV MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL 250 260 270 280 290 300 PPALHGSRGA DCVLTSGEIA QIMEQGDLSV RDAAVDTLFG DLKEDKVTRH DGASSDGHLA 310 320 330 340 350 360 HIFRHAAKEL FNEDVEEVTY RALRNKDFQE VTLEKNGEVV LRFAAAYGFR NIQNMILKLK 370 380 390 400 410 420 KGKFPFHFVE VLACAGGCLN GRGQAQTPDG HADKALLRQM EGIYADIPVR RPESSAHVQE 430 440 450 LYQEWLEGIN SPKAREVLHT TYQSQERGTH SLDIKW
	Isoform 2 [UniParc]. Checksum: 79A6F299C2CAAEF1	FASTA	502	56,161
	10 20 30 40 50 60 MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL SDCLACDSCM 70 80 90 100 110 120 TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP QSLPYFAAKF NLSVTDASRR 130 140 150 160 170 180 LCGFLKSLGV HYVFDTTIAA DFSILESQKE FVRRYRQHSE EERTLPMLTS ACPGWVRYAE 190 200 210 220 230 240 RVLGRPITAH LCTAKSPQQV MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL 250 260 270 280 290 300 PPALHGSRGA DCVLTSEISQ AWWCTPVITA TREAAARESL EPGRQRLQRD KIAPLDSSLG 310 320 330 340 350 360 GGGEIAQIME QGDLSVRDAA VDTLFGDLKE DKVTRHDGAS SDGHLAHIFR HAAKELFNED 370 380 390 400 410 420 VEEVTYRALR NKDFQEVTLE KNGEVVLRFA AAYGFRNIQN MILKLKKGKF PFHFVEVLAC 430 440 450 460 470 480 AGGCLNGRGQ AQTPDGHADK ALLRQMEGIY ADIPVRRPES SAHVQELYQE WLEGINSPKA 490 500 REVLHTTYQS QERGTHSLDI KW
	Isoform 3 [UniParc]. Checksum: 42AD67C382D83D7C	FASTA	408	45,796
	10 20 30 40 50 60 MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKCDT SKHKVLVVSV CPQSLPYFAA 70 80 90 100 110 120 KFNLSVTDAS RRLCGFLKSL GVHYVFDTTI AADFSILESQ KEFVRRYRQH SEEERTLPML 130 140 150 160 170 180 TSACPGWVRY AERVLGRPIT AHLCTAKSPQ QVMGSLVKDY FARQQNLSPE KIFHVIVAPC 190 200 210 220 230 240 YDKKLEALQE SLPPALHGSR GADCVLTSGE IAQIMEQGDL SVRDAAVDTL FGDLKEDKVT 250 260 270 280 290 300 RHDGASSDGH LAHIFRHAAK ELFNEDVEEV TYRALRNKDF QEVTLEKNGE VVLRFAAAYG 310 320 330 340 350 360 FRNIQNMILK LKKGKFPFHF VEVLACAGGC LNGRGQAQTP DGHADKALLR QMEGIYADIP 370 380 390 400 VRRPESSAHV QELYQEWLEG INSPKAREVL HTTYQSQERG THSLDIKW
	Isoform 4 [UniParc]. Checksum: F7CC7AE3834362FA	FASTA	397	44,681
	10 20 30 40 50 60 MTAEEGVQLS QQNAKDFFRV LNLNKKCDTS KHKVLVVSVC PQSLPYFAAK FNLSVTDASR 70 80 90 100 110 120 RLCGFLKSLG VHYVFDTTIA ADFSILESQK EFVRRYRQHS EEERTLPMLT SACPGWVRYA 130 140 150 160 170 180 ERVLGRPITA HLCTAKSPQQ VMGSLVKDYF ARQQNLSPEK IFHVIVAPCY DKKLEALQES 190 200 210 220 230 240 LPPALHGSRG ADCVLTSGEI AQIMEQGDLS VRDAAVDTLF GDLKEDKVTR HDGASSDGHL 250 260 270 280 290 300 AHIFRHAAKE LFNEDVEEVT YRALRNKDFQ EVTLEKNGEV VLRFAAAYGF RNIQNMILKL 310 320 330 340 350 360 KKGKFPFHFV EVLACAGGCL NGRGQAQTPD GHADKALLRQ MEGIYADIPV RRPESSAHVQ 370 380 390 ELYQEWLEGI NSPKAREVLH TTYQSQERGT HSLDIKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prenylated prelamin A interacts with Narf, a novel nuclear protein." Barton R.M., Worman H.J. J. Biol. Chem. 274:30008-30018(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH LMNA.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4). Tissue: Embryo and Skeletal muscle.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Lung and Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF128406 mRNA. Translation: AAD51446.1. AK000929 mRNA. Translation: BAA91432.1. AK056966 mRNA. Translation: BAG51834.1. AC124287 Genomic DNA. No translation available. AC132938 Genomic DNA. No translation available. CH471099 Genomic DNA. Translation: EAW89794.1. CH471099 Genomic DNA. Translation: EAW89798.1. BC000438 mRNA. Translation: AAH00438.1. BC016440 mRNA. Translation: AAH16440.1.
IPI	IPI00011237. IPI00185459. IPI00744719.
RefSeq	NP_001033707.1. NM_001038618.2. NP_001077077.1. NM_001083608.1. NP_036468.1. NM_012336.3. NP_114174.1. NM_031968.2.
UniGene	Hs.256526. Hs.600304.
3D structure databases
ProteinModelPortal	Q9UHQ1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UHQ1. 2 interactions.
MINT	MINT-8330046.
STRING	9606.ENSP00000309899.
PTM databases
PhosphoSite	Q9UHQ1.
Polymorphism databases
DMDM	74735021.
Proteomic databases
PaxDb	Q9UHQ1.
PRIDE	Q9UHQ1.
Protocols and materials databases
DNASU	26502.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000309794; ENSP00000309899; ENSG00000141562. ENST00000345415; ENSP00000283996; ENSG00000141562. ENST00000390006; ENSP00000374656; ENSG00000141562.
GeneID	26502.
KEGG	hsa:26502.
UCSC	uc002kff.4. human. uc002kfj.4. human. uc010dit.3. human.
Organism-specific databases
CTD	26502.
GeneCards	GC17P080416.
HGNC	HGNC:29916. NARF.
MIM	605349. gene.
neXtProt	NX_Q9UHQ1.
PharmGKB	PA142671294.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4624.
HOGENOM	HOG000191744.
HOVERGEN	HBG055005.
Gene expression databases
ArrayExpress	Q9UHQ1.
Bgee	Q9UHQ1.
CleanEx	HS_NARF.
Genevestigator	Q9UHQ1.
Family and domain databases
Gene3D	4.10.260.20. 1 hit.
InterPro	IPR009016. Fe_hydrogenase. IPR004108. Fe_hydrogenase_lsu_C. IPR003149. Fe_hydrogenase_ssu-like. [Graphical view]
Pfam	PF02906. Fe_hyd_lg_C. 1 hit. PF02256. Fe_hyd_SSU. 1 hit. [Graphical view]
SMART	SM00902. Fe_hyd_SSU. 1 hit. [Graphical view]
SUPFAM	SSF53920. Fe_hydrog. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	26502.
NextBio	48768.
SOURCE	Search...

Entry information

Entry name

NARF_HUMAN

Accession

Primary (citable) accession number: Q9UHQ1
Secondary accession number(s): A6NCJ3 , B3KPX2, K4DI98, Q96AY9, Q9BWC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.