Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear prelamin A recognition factor

Gene

NARF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • lamin binding Source: HGNC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear prelamin A recognition factor
Alternative name(s):
Iron-only hydrogenase-like protein 2
Short name:
IOP2
Gene namesi
Name:NARF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:29916. NARF.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • lamin filament Source: HGNC
  • nuclear lamina Source: ProtInc
  • nuclear lumen Source: HGNC
  • nucleolus Source: HPA
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671294.

Polymorphism and mutation databases

BioMutaiNARF.
DMDMi74735021.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Nuclear prelamin A recognition factorPRO_0000288479Add
BLAST

Proteomic databases

MaxQBiQ9UHQ1.
PaxDbiQ9UHQ1.
PRIDEiQ9UHQ1.

PTM databases

PhosphoSiteiQ9UHQ1.

Expressioni

Tissue specificityi

Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.1 Publication

Gene expression databases

BgeeiQ9UHQ1.
CleanExiHS_NARF.
ExpressionAtlasiQ9UHQ1. baseline and differential.
GenevestigatoriQ9UHQ1.

Interactioni

Subunit structurei

Interacts with LMNA and binds to the farnesylated C-terminal domain.1 Publication

Protein-protein interaction databases

BioGridi117709. 9 interactions.
IntActiQ9UHQ1. 4 interactions.
MINTiMINT-8330046.
STRINGi9606.ENSP00000309899.

Structurei

3D structure databases

ProteinModelPortaliQ9UHQ1.
SMRiQ9UHQ1. Positions 35-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NARF family.Curated

Phylogenomic databases

eggNOGiCOG4624.
GeneTreeiENSGT00390000017231.
HOGENOMiHOG000191744.
HOVERGENiHBG055005.
InParanoidiQ9UHQ1.
PhylomeDBiQ9UHQ1.
TreeFamiTF106273.

Family and domain databases

Gene3Di4.10.260.20. 1 hit.
InterProiIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu-like.
[Graphical view]
PfamiPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTiSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMiSSF53920. SSF53920. 2 hits.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UHQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL
60 70 80 90 100
SDCLACDSCM TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP
110 120 130 140 150
QSLPYFAAKF NLSVTDASRR LCGFLKSLGV HYVFDTTIAA DFSILESQKE
160 170 180 190 200
FVRRYRQHSE EERTLPMLTS ACPGWVRYAE RVLGRPITAH LCTAKSPQQV
210 220 230 240 250
MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL PPALHGSRGA
260 270 280 290 300
DCVLTSGEIA QIMEQGDLSV RDAAVDTLFG DLKEDKVTRH DGASSDGHLA
310 320 330 340 350
HIFRHAAKEL FNEDVEEVTY RALRNKDFQE VTLEKNGEVV LRFAAAYGFR
360 370 380 390 400
NIQNMILKLK KGKFPFHFVE VLACAGGCLN GRGQAQTPDG HADKALLRQM
410 420 430 440 450
EGIYADIPVR RPESSAHVQE LYQEWLEGIN SPKAREVLHT TYQSQERGTH

SLDIKW
Length:456
Mass (Da):51,156
Last modified:May 1, 2000 - v1
Checksum:iF615D812E7FC7D0F
GO
Isoform 2 (identifier: Q9UHQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: S → SEISQAWWCTPVITATREAAARESLEPGRQRLQRDKIAPLDSSLGGG

Show »
Length:502
Mass (Da):56,161
Checksum:i79A6F299C2CAAEF1
GO
Isoform 3 (identifier: Q9UHQ1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-85: Missing.

Show »
Length:408
Mass (Da):45,796
Checksum:i42AD67C382D83D7C
GO
Isoform 4 (identifier: Q9UHQ1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Note: No experimental confirmation available.

Show »
Length:397
Mass (Da):44,681
Checksum:iF7CC7AE3834362FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti154 – 1541R → L in BAG51834 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5959Missing in isoform 4. 1 PublicationVSP_046371Add
BLAST
Alternative sequencei38 – 8548Missing in isoform 3. 1 PublicationVSP_025691Add
BLAST
Alternative sequencei256 – 2561S → SEISQAWWCTPVITATREAA ARESLEPGRQRLQRDKIAPL DSSLGGG in isoform 2. 1 PublicationVSP_025690

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128406 mRNA. Translation: AAD51446.1.
AK000929 mRNA. Translation: BAA91432.1.
AK056966 mRNA. Translation: BAG51834.1.
AC124287 Genomic DNA. No translation available.
AC132938 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89794.1.
CH471099 Genomic DNA. Translation: EAW89798.1.
BC000438 mRNA. Translation: AAH00438.1.
BC016440 mRNA. Translation: AAH16440.1.
CCDSiCCDS32777.1. [Q9UHQ1-1]
CCDS42403.1. [Q9UHQ1-3]
CCDS42404.1. [Q9UHQ1-4]
RefSeqiNP_001033707.1. NM_001038618.2. [Q9UHQ1-4]
NP_001077077.1. NM_001083608.1. [Q9UHQ1-3]
NP_036468.1. NM_012336.3. [Q9UHQ1-1]
NP_114174.1. NM_031968.2. [Q9UHQ1-2]
XP_005256397.1. XM_005256340.2. [Q9UHQ1-4]
UniGeneiHs.256526.
Hs.600304.

Genome annotation databases

EnsembliENST00000309794; ENSP00000309899; ENSG00000141562. [Q9UHQ1-1]
ENST00000345415; ENSP00000283996; ENSG00000141562. [Q9UHQ1-3]
ENST00000390006; ENSP00000374656; ENSG00000141562. [Q9UHQ1-4]
GeneIDi26502.
KEGGihsa:26502.
UCSCiuc002kff.4. human. [Q9UHQ1-1]
uc002kfj.4. human. [Q9UHQ1-3]
uc010dit.3. human. [Q9UHQ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128406 mRNA. Translation: AAD51446.1.
AK000929 mRNA. Translation: BAA91432.1.
AK056966 mRNA. Translation: BAG51834.1.
AC124287 Genomic DNA. No translation available.
AC132938 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89794.1.
CH471099 Genomic DNA. Translation: EAW89798.1.
BC000438 mRNA. Translation: AAH00438.1.
BC016440 mRNA. Translation: AAH16440.1.
CCDSiCCDS32777.1. [Q9UHQ1-1]
CCDS42403.1. [Q9UHQ1-3]
CCDS42404.1. [Q9UHQ1-4]
RefSeqiNP_001033707.1. NM_001038618.2. [Q9UHQ1-4]
NP_001077077.1. NM_001083608.1. [Q9UHQ1-3]
NP_036468.1. NM_012336.3. [Q9UHQ1-1]
NP_114174.1. NM_031968.2. [Q9UHQ1-2]
XP_005256397.1. XM_005256340.2. [Q9UHQ1-4]
UniGeneiHs.256526.
Hs.600304.

3D structure databases

ProteinModelPortaliQ9UHQ1.
SMRiQ9UHQ1. Positions 35-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117709. 9 interactions.
IntActiQ9UHQ1. 4 interactions.
MINTiMINT-8330046.
STRINGi9606.ENSP00000309899.

PTM databases

PhosphoSiteiQ9UHQ1.

Polymorphism and mutation databases

BioMutaiNARF.
DMDMi74735021.

Proteomic databases

MaxQBiQ9UHQ1.
PaxDbiQ9UHQ1.
PRIDEiQ9UHQ1.

Protocols and materials databases

DNASUi26502.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309794; ENSP00000309899; ENSG00000141562. [Q9UHQ1-1]
ENST00000345415; ENSP00000283996; ENSG00000141562. [Q9UHQ1-3]
ENST00000390006; ENSP00000374656; ENSG00000141562. [Q9UHQ1-4]
GeneIDi26502.
KEGGihsa:26502.
UCSCiuc002kff.4. human. [Q9UHQ1-1]
uc002kfj.4. human. [Q9UHQ1-3]
uc010dit.3. human. [Q9UHQ1-2]

Organism-specific databases

CTDi26502.
GeneCardsiGC17P080416.
HGNCiHGNC:29916. NARF.
MIMi605349. gene.
neXtProtiNX_Q9UHQ1.
PharmGKBiPA142671294.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG4624.
GeneTreeiENSGT00390000017231.
HOGENOMiHOG000191744.
HOVERGENiHBG055005.
InParanoidiQ9UHQ1.
PhylomeDBiQ9UHQ1.
TreeFamiTF106273.

Miscellaneous databases

GeneWikiiNARF.
GenomeRNAii26502.
NextBioi48768.
PROiQ9UHQ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UHQ1.
CleanExiHS_NARF.
ExpressionAtlasiQ9UHQ1. baseline and differential.
GenevestigatoriQ9UHQ1.

Family and domain databases

Gene3Di4.10.260.20. 1 hit.
InterProiIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu-like.
[Graphical view]
PfamiPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTiSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMiSSF53920. SSF53920. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prenylated prelamin A interacts with Narf, a novel nuclear protein."
    Barton R.M., Worman H.J.
    J. Biol. Chem. 274:30008-30018(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH LMNA.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Embryo and Skeletal muscle.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Lung and Placenta.

Entry informationi

Entry nameiNARF_HUMAN
AccessioniPrimary (citable) accession number: Q9UHQ1
Secondary accession number(s): A6NCJ3
, B3KPX2, K4DI98, Q96AY9, Q9BWC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.