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Q9UHQ1 (NARF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear prelamin A recognition factor
Alternative name(s):
Iron-only hydrogenase-like protein 2
Short name=IOP2
Gene names
Name:NARF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with LMNA and binds to the farnesylated C-terminal domain. Ref.1

Subcellular location

Nucleus Ref.1.

Tissue specificity

Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain. Ref.1

Sequence similarities

Belongs to the NARF family.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentlamin filament

Inferred from direct assay Ref.1. Source: HGNC

nuclear lamina

Traceable author statement Ref.1. Source: ProtInc

nuclear lumen

Inferred from direct assay Ref.1. Source: HGNC

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionlamin binding

Inferred from physical interaction Ref.1. Source: HGNC

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UHQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UHQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-256: S → SEISQAWWCTPVITATREAAARESLEPGRQRLQRDKIAPLDSSLGGG
Isoform 3 (identifier: Q9UHQ1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     38-85: Missing.
Isoform 4 (identifier: Q9UHQ1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456Nuclear prelamin A recognition factor
PRO_0000288479

Natural variations

Alternative sequence1 – 5959Missing in isoform 4.
VSP_046371
Alternative sequence38 – 8548Missing in isoform 3.
VSP_025691
Alternative sequence2561S → SEISQAWWCTPVITATREAA ARESLEPGRQRLQRDKIAPL DSSLGGG in isoform 2.
VSP_025690

Experimental info

Sequence conflict1541R → L in BAG51834. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F615D812E7FC7D0F

FASTA45651,156
        10         20         30         40         50         60 
MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL SDCLACDSCM 

        70         80         90        100        110        120 
TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP QSLPYFAAKF NLSVTDASRR 

       130        140        150        160        170        180 
LCGFLKSLGV HYVFDTTIAA DFSILESQKE FVRRYRQHSE EERTLPMLTS ACPGWVRYAE 

       190        200        210        220        230        240 
RVLGRPITAH LCTAKSPQQV MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL 

       250        260        270        280        290        300 
PPALHGSRGA DCVLTSGEIA QIMEQGDLSV RDAAVDTLFG DLKEDKVTRH DGASSDGHLA 

       310        320        330        340        350        360 
HIFRHAAKEL FNEDVEEVTY RALRNKDFQE VTLEKNGEVV LRFAAAYGFR NIQNMILKLK 

       370        380        390        400        410        420 
KGKFPFHFVE VLACAGGCLN GRGQAQTPDG HADKALLRQM EGIYADIPVR RPESSAHVQE 

       430        440        450 
LYQEWLEGIN SPKAREVLHT TYQSQERGTH SLDIKW 

« Hide

Isoform 2 [UniParc].

Checksum: 79A6F299C2CAAEF1
Show »

FASTA50256,161
Isoform 3 [UniParc].

Checksum: 42AD67C382D83D7C
Show »

FASTA40845,796
Isoform 4 [UniParc].

Checksum: F7CC7AE3834362FA
Show »

FASTA39744,681

References

« Hide 'large scale' references
[1]"Prenylated prelamin A interacts with Narf, a novel nuclear protein."
Barton R.M., Worman H.J.
J. Biol. Chem. 274:30008-30018(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH LMNA.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Embryo and Skeletal muscle.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Lung and Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF128406 mRNA. Translation: AAD51446.1.
AK000929 mRNA. Translation: BAA91432.1.
AK056966 mRNA. Translation: BAG51834.1.
AC124287 Genomic DNA. No translation available.
AC132938 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89794.1.
CH471099 Genomic DNA. Translation: EAW89798.1.
BC000438 mRNA. Translation: AAH00438.1.
BC016440 mRNA. Translation: AAH16440.1.
CCDSCCDS32777.1. [Q9UHQ1-1]
CCDS42403.1. [Q9UHQ1-3]
CCDS42404.1. [Q9UHQ1-4]
RefSeqNP_001033707.1. NM_001038618.2. [Q9UHQ1-4]
NP_001077077.1. NM_001083608.1. [Q9UHQ1-3]
NP_036468.1. NM_012336.3. [Q9UHQ1-1]
NP_114174.1. NM_031968.2. [Q9UHQ1-2]
XP_005256397.1. XM_005256340.2. [Q9UHQ1-4]
UniGeneHs.256526.
Hs.600304.

3D structure databases

ProteinModelPortalQ9UHQ1.
SMRQ9UHQ1. Positions 51-443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117709. 9 interactions.
IntActQ9UHQ1. 3 interactions.
MINTMINT-8330046.
STRING9606.ENSP00000309899.

PTM databases

PhosphoSiteQ9UHQ1.

Polymorphism databases

DMDM74735021.

Proteomic databases

MaxQBQ9UHQ1.
PaxDbQ9UHQ1.
PRIDEQ9UHQ1.

Protocols and materials databases

DNASU26502.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309794; ENSP00000309899; ENSG00000141562. [Q9UHQ1-1]
ENST00000345415; ENSP00000283996; ENSG00000141562. [Q9UHQ1-3]
ENST00000390006; ENSP00000374656; ENSG00000141562. [Q9UHQ1-4]
GeneID26502.
KEGGhsa:26502.
UCSCuc002kff.4. human. [Q9UHQ1-1]
uc002kfj.4. human. [Q9UHQ1-3]
uc010dit.3. human. [Q9UHQ1-2]

Organism-specific databases

CTD26502.
GeneCardsGC17P080416.
HGNCHGNC:29916. NARF.
HPAHPA052371.
MIM605349. gene.
neXtProtNX_Q9UHQ1.
PharmGKBPA142671294.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4624.
HOGENOMHOG000191744.
HOVERGENHBG055005.
PhylomeDBQ9UHQ1.
TreeFamTF106273.

Gene expression databases

ArrayExpressQ9UHQ1.
BgeeQ9UHQ1.
CleanExHS_NARF.
GenevestigatorQ9UHQ1.

Family and domain databases

Gene3D4.10.260.20. 1 hit.
InterProIPR009016. Fe_hydrogenase.
IPR004108. Fe_hydrogenase_lsu_C.
IPR003149. Fe_hydrogenase_ssu-like.
[Graphical view]
PfamPF02906. Fe_hyd_lg_C. 1 hit.
PF02256. Fe_hyd_SSU. 1 hit.
[Graphical view]
SMARTSM00902. Fe_hyd_SSU. 1 hit.
[Graphical view]
SUPFAMSSF53920. SSF53920. 2 hits.
ProtoNetSearch...

Other

GeneWikiNARF.
GenomeRNAi26502.
NextBio48768.
PROQ9UHQ1.
SOURCESearch...

Entry information

Entry nameNARF_HUMAN
AccessionPrimary (citable) accession number: Q9UHQ1
Secondary accession number(s): A6NCJ3 expand/collapse secondary AC list , B3KPX2, K4DI98, Q96AY9, Q9BWC6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM