ID   OPN4_HUMAN              Reviewed;         478 AA.
AC   Q9UHM6; B7ZLB3; Q14D01; Q2PP22; Q8NGQ9;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   24-JAN-2024, entry version 172.
DE   RecName: Full=Melanopsin;
DE   AltName: Full=Opsin-4;
GN   Name=OPN4; Synonyms=MOP;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX   PubMed=10632589; DOI=10.1523/jneurosci.20-02-00600.2000;
RA   Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F.,
RA   Rollag M.D.;
RT   "A novel human opsin in the inner retina.";
RL   J. Neurosci. 20:600-605(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ILE-394.
RG   SeattleSNPs variation discovery resource;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP   LEU-10.
RC   TISSUE=Brain, and Brain cortex;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-418, AND VARIANT LEU-10.
RA   Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA   Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT   "Genome-wide discovery and analysis of human seven transmembrane helix
RT   receptor genes.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION.
RX   PubMed=15674244; DOI=10.1038/nature03344;
RA   Melyan Z., Tarttelin E.E., Bellingham J., Lucas R.J., Hankins M.W.;
RT   "Addition of human melanopsin renders mammalian cells photoresponsive.";
RL   Nature 433:741-745(2005).
CC   -!- FUNCTION: Photoreceptor that binds cis-retinaldehydes
CC       (PubMed:15674244). Contributes to pupillar reflex, photoentrainment and
CC       other non-image forming responses to light (By similarity). May be
CC       involved in the optokinetic visual tracking response (By similarity).
CC       May be involved in the regulation of retinal hyaloid vessel growth and
CC       regression (By similarity). {ECO:0000250|UniProtKB:Q9QXZ9,
CC       ECO:0000269|PubMed:15674244}.
CC   -!- INTERACTION:
CC       Q9UHM6; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-18058356, EBI-947187;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9QXZ9};
CC       Multi-pass membrane protein {ECO:0000255}. Cell projection, axon
CC       {ECO:0000250|UniProtKB:Q9QXZ9}. Cell projection, dendrite
CC       {ECO:0000250|UniProtKB:Q9QXZ9}. Perikaryon
CC       {ECO:0000250|UniProtKB:Q9QXZ9}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9UHM6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9UHM6-2; Sequence=VSP_041123;
CC   -!- TISSUE SPECIFICITY: Expressed in the retina.
CC       {ECO:0000269|PubMed:10632589}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. Opsin
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC05951.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=SeattleSNPs;
CC       URL="http://pga.gs.washington.edu/data/opn4/";
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DR   EMBL; AF147788; AAF24978.1; -; Genomic_DNA.
DR   EMBL; DQ314804; ABC40723.1; -; Genomic_DNA.
DR   EMBL; BC113558; AAI13559.1; -; mRNA.
DR   EMBL; BC143688; AAI43689.1; -; mRNA.
DR   EMBL; AB065730; BAC05951.1; ALT_SEQ; Genomic_DNA.
DR   CCDS; CCDS31237.1; -. [Q9UHM6-2]
DR   CCDS; CCDS7376.1; -. [Q9UHM6-1]
DR   RefSeq; NP_001025186.1; NM_001030015.2. [Q9UHM6-2]
DR   RefSeq; NP_150598.1; NM_033282.3. [Q9UHM6-1]
DR   AlphaFoldDB; Q9UHM6; -.
DR   SMR; Q9UHM6; -.
DR   BioGRID; 125146; 68.
DR   IntAct; Q9UHM6; 4.
DR   STRING; 9606.ENSP00000361141; -.
DR   TCDB; 9.A.14.1.8; the g-protein-coupled receptor (gpcr) family.
DR   iPTMnet; Q9UHM6; -.
DR   PhosphoSitePlus; Q9UHM6; -.
DR   BioMuta; OPN4; -.
DR   DMDM; 13632136; -.
DR   MassIVE; Q9UHM6; -.
DR   PaxDb; 9606-ENSP00000361141; -.
DR   PeptideAtlas; Q9UHM6; -.
DR   Antibodypedia; 30087; 108 antibodies from 23 providers.
DR   DNASU; 94233; -.
DR   Ensembl; ENST00000241891.10; ENSP00000241891.5; ENSG00000122375.13. [Q9UHM6-1]
DR   Ensembl; ENST00000372071.7; ENSP00000361141.2; ENSG00000122375.13. [Q9UHM6-2]
DR   GeneID; 94233; -.
DR   KEGG; hsa:94233; -.
DR   MANE-Select; ENST00000241891.10; ENSP00000241891.5; NM_033282.4; NP_150598.1.
DR   UCSC; uc001kdp.4; human. [Q9UHM6-1]
DR   AGR; HGNC:14449; -.
DR   CTD; 94233; -.
DR   DisGeNET; 94233; -.
DR   GeneCards; OPN4; -.
DR   HGNC; HGNC:14449; OPN4.
DR   HPA; ENSG00000122375; Tissue enhanced (adipose tissue, brain, skeletal muscle).
DR   MIM; 606665; gene.
DR   neXtProt; NX_Q9UHM6; -.
DR   OpenTargets; ENSG00000122375; -.
DR   PharmGKB; PA31940; -.
DR   VEuPathDB; HostDB:ENSG00000122375; -.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT01100000263502; -.
DR   HOGENOM; CLU_009579_3_12_1; -.
DR   InParanoid; Q9UHM6; -.
DR   OMA; WKMAKIV; -.
DR   OrthoDB; 3432893at2759; -.
DR   PhylomeDB; Q9UHM6; -.
DR   TreeFam; TF324998; -.
DR   PathwayCommons; Q9UHM6; -.
DR   Reactome; R-HSA-416476; G alpha (q) signalling events.
DR   Reactome; R-HSA-419771; Opsins.
DR   SignaLink; Q9UHM6; -.
DR   BioGRID-ORCS; 94233; 17 hits in 1134 CRISPR screens.
DR   GeneWiki; Melanopsin; -.
DR   GenomeRNAi; 94233; -.
DR   Pharos; Q9UHM6; Tbio.
DR   PRO; PR:Q9UHM6; -.
DR   Proteomes; UP000005640; Chromosome 10.
DR   RNAct; Q9UHM6; Protein.
DR   Bgee; ENSG00000122375; Expressed in gastrocnemius and 63 other cell types or tissues.
DR   ExpressionAtlas; Q9UHM6; baseline and differential.
DR   GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR   GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; TAS:UniProtKB.
DR   GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR   GO; GO:0097381; C:photoreceptor disc membrane; TAS:Reactome.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:1990913; C:sperm head plasma membrane; IEA:Ensembl.
DR   GO; GO:0005502; F:11-cis retinal binding; IDA:UniProtKB.
DR   GO; GO:0008020; F:G protein-coupled photoreceptor activity; IDA:UniProtKB.
DR   GO; GO:0071482; P:cellular response to light stimulus; IBA:GO_Central.
DR   GO; GO:0050960; P:detection of temperature stimulus involved in thermoception; IEA:Ensembl.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:1990384; P:hyaloid vascular plexus regression; ISS:UniProtKB.
DR   GO; GO:0007634; P:optokinetic behavior; ISS:UniProtKB.
DR   GO; GO:0007602; P:phototransduction; IDA:UniProtKB.
DR   GO; GO:0042752; P:regulation of circadian rhythm; ISS:UniProtKB.
DR   GO; GO:0060041; P:retina development in camera-type eye; IEA:Ensembl.
DR   GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR   GO; GO:0043052; P:thermotaxis; IEA:Ensembl.
DR   GO; GO:0007601; P:visual perception; IEA:InterPro.
DR   CDD; cd15336; 7tmA_Melanopsin; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR001760; Opsin.
DR   InterPro; IPR027430; Retinal_BS.
DR   PANTHER; PTHR24240:SF72; MELANOPSIN; 1.
DR   PANTHER; PTHR24240; OPSIN; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR00238; OPSIN.
DR   SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   PROSITE; PS00238; OPSIN; 1.
DR   Genevisible; Q9UHM6; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Biological rhythms; Cell membrane; Cell projection;
KW   Chromophore; Disulfide bond; G-protein coupled receptor; Membrane;
KW   Photoreceptor protein; Receptor; Reference proteome; Retinal protein;
KW   Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT   CHAIN           1..478
FT                   /note="Melanopsin"
FT                   /id="PRO_0000197815"
FT   TOPO_DOM        1..72
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..107
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        129..144
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        166..188
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        210..238
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        260..296
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        318..332
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        354..478
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          440..478
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         340
FT                   /note="N6-(retinylidene)lysine"
FT   DISULFID        143..221
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VAR_SEQ         97
FT                   /note="R -> RAVLRGVTVMMQ (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_041123"
FT   VARIANT         10
FT                   /note="P -> L (in dbSNP:rs2675703)"
FT                   /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4"
FT                   /id="VAR_029774"
FT   VARIANT         394
FT                   /note="T -> I (in dbSNP:rs1079610)"
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="VAR_020430"
FT   VARIANT         444
FT                   /note="G -> D (in dbSNP:rs12262894)"
FT                   /id="VAR_029775"
SQ   SEQUENCE   478 AA;  52635 MW;  A8022F5B5AA1198F CRC64;
     MNPPSGPRVP PSPTQEPSCM ATPAPPSWWD SSQSSISSLG RLPSISPTAP GTWAAAWVPL
     PTVDVPDHAH YTLGTVILLV GLTGMLGNLT VIYTFCRSRS LRTPANMFII NLAVSDFLMS
     FTQAPVFFTS SLYKQWLFGE TGCEFYAFCG ALFGISSMIT LTAIALDRYL VITRPLATFG
     VASKRRAAFV LLGVWLYALA WSLPPFFGWS AYVPEGLLTS CSWDYMSFTP AVRAYTMLLC
     CFVFFLPLLI IIYCYIFIFR AIRETGRALQ TFGACKGNGE SLWQRQRLQS ECKMAKIMLL
     VILLFVLSWA PYSAVALVAF AGYAHVLTPY MSSVPAVIAK ASAIHNPIIY AITHPKYRVA
     IAQHLPCLGV LLGVSRRHSR PYPSYRSTHR STLTSHTSNL SWISIRRRQE SLGSESEVGW
     THMEAAAVWG AAQQANGRSL YGQGLEDLEA KAPPRPQGHE AETPGKTKGL IPSQDPRM
//