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Q9UHM6 (OPN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanopsin
Alternative name(s):
Opsin-4
Gene names
Name:OPN4
Synonyms:MOP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal By similarity. Ref.1 Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells By similarity.

Tissue specificity

Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Sequence caution

The sequence BAC05951.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UHM6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UHM6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-97: R → RAVLRGVTVMMQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Melanopsin
PRO_0000197815

Regions

Topological domain1 – 7272Extracellular Potential
Transmembrane73 – 9321Helical; Name=1; Potential
Topological domain94 – 10714Cytoplasmic Potential
Transmembrane108 – 12821Helical; Name=2; Potential
Topological domain129 – 14416Extracellular Potential
Transmembrane145 – 16521Helical; Name=3; Potential
Topological domain166 – 18823Cytoplasmic Potential
Transmembrane189 – 20921Helical; Name=4; Potential
Topological domain210 – 23829Extracellular Potential
Transmembrane239 – 25921Helical; Name=5; Potential
Topological domain260 – 29637Cytoplasmic Potential
Transmembrane297 – 31721Helical; Name=6; Potential
Topological domain318 – 33215Extracellular Potential
Transmembrane333 – 35321Helical; Name=7; Potential
Topological domain354 – 478125Cytoplasmic Potential

Amino acid modifications

Modified residue3401N6-(retinylidene)lysine
Disulfide bond143 ↔ 221 Potential

Natural variations

Alternative sequence971R → RAVLRGVTVMMQ in isoform 2.
VSP_041123
Natural variant101P → L. Ref.3 Ref.4
Corresponds to variant rs2675703 [ dbSNP | Ensembl ].
VAR_029774
Natural variant3941T → I. Ref.2
Corresponds to variant rs1079610 [ dbSNP | Ensembl ].
VAR_020430
Natural variant4441G → D.
Corresponds to variant rs12262894 [ dbSNP | Ensembl ].
VAR_029775

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: A8022F5B5AA1198F

FASTA47852,635
        10         20         30         40         50         60 
MNPPSGPRVP PSPTQEPSCM ATPAPPSWWD SSQSSISSLG RLPSISPTAP GTWAAAWVPL 

        70         80         90        100        110        120 
PTVDVPDHAH YTLGTVILLV GLTGMLGNLT VIYTFCRSRS LRTPANMFII NLAVSDFLMS 

       130        140        150        160        170        180 
FTQAPVFFTS SLYKQWLFGE TGCEFYAFCG ALFGISSMIT LTAIALDRYL VITRPLATFG 

       190        200        210        220        230        240 
VASKRRAAFV LLGVWLYALA WSLPPFFGWS AYVPEGLLTS CSWDYMSFTP AVRAYTMLLC 

       250        260        270        280        290        300 
CFVFFLPLLI IIYCYIFIFR AIRETGRALQ TFGACKGNGE SLWQRQRLQS ECKMAKIMLL 

       310        320        330        340        350        360 
VILLFVLSWA PYSAVALVAF AGYAHVLTPY MSSVPAVIAK ASAIHNPIIY AITHPKYRVA 

       370        380        390        400        410        420 
IAQHLPCLGV LLGVSRRHSR PYPSYRSTHR STLTSHTSNL SWISIRRRQE SLGSESEVGW 

       430        440        450        460        470 
THMEAAAVWG AAQQANGRSL YGQGLEDLEA KAPPRPQGHE AETPGKTKGL IPSQDPRM 

« Hide

Isoform 2 [UniParc].

Checksum: 48D2D86479D9EC72
Show »

FASTA48953,822

References

« Hide 'large scale' references
[1]"A novel human opsin in the inner retina."
Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F., Rollag M.D.
J. Neurosci. 20:600-605(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
[2]SeattleSNPs variation discovery resource
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ILE-394.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT LEU-10.
Tissue: Brain and Brain cortex.
[4]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-418, VARIANT LEU-10.
[5]"Addition of human melanopsin renders mammalian cells photoresponsive."
Melyan Z., Tarttelin E.E., Bellingham J., Lucas R.J., Hankins M.W.
Nature 433:741-745(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF147788 Genomic DNA. Translation: AAF24978.1.
DQ314804 Genomic DNA. Translation: ABC40723.1.
BC113558 mRNA. Translation: AAI13559.1.
BC143688 mRNA. Translation: AAI43689.1.
AB065730 Genomic DNA. Translation: BAC05951.1. Sequence problems.
CCDSCCDS31237.1. [Q9UHM6-2]
CCDS7376.1. [Q9UHM6-1]
RefSeqNP_001025186.1. NM_001030015.2. [Q9UHM6-2]
NP_150598.1. NM_033282.3. [Q9UHM6-1]
UniGeneHs.283922.

3D structure databases

ProteinModelPortalQ9UHM6.
SMRQ9UHM6. Positions 65-366.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000361141.

Protein family/group databases

TCDB9.A.14.1.8. the g-protein-coupled receptor (gpcr) family.
GPCRDBSearch...

Polymorphism databases

DMDM13632136.

Proteomic databases

PaxDbQ9UHM6.
PRIDEQ9UHM6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000241891; ENSP00000241891; ENSG00000122375. [Q9UHM6-1]
ENST00000372071; ENSP00000361141; ENSG00000122375. [Q9UHM6-2]
GeneID94233.
KEGGhsa:94233.
UCSCuc001kdp.3. human. [Q9UHM6-2]
uc001kdq.3. human. [Q9UHM6-1]

Organism-specific databases

CTD94233.
GeneCardsGC10P088417.
HGNCHGNC:14449. OPN4.
HPAHPA039147.
MIM606665. gene.
neXtProtNX_Q9UHM6.
PharmGKBPA31940.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG255465.
HOGENOMHOG000264261.
HOVERGENHBG007871.
KOK04255.
OrthoDBEOG7CNZG2.
PhylomeDBQ9UHM6.
TreeFamTF324998.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ9UHM6.
BgeeQ9UHM6.
CleanExHS_OPN4.
GenevestigatorQ9UHM6.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMelanopsin.
GenomeRNAi94233.
NextBio78466.
PROQ9UHM6.
SOURCESearch...

Entry information

Entry nameOPN4_HUMAN
AccessionPrimary (citable) accession number: Q9UHM6
Secondary accession number(s): B7ZLB3 expand/collapse secondary AC list , Q14D01, Q2PP22, Q8NGQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries