Q9UHL0 (DDX25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DDX25
EC=3.6.4.13

Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase

Gene names

Name:	DDX25
Synonyms:	GRTH

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development By similarity. Ref.1
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Cytoplasm. Nucleus By similarity. Note: Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids By similarity. Ref.1
Tissue specificity	Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus. Ref.1 Ref.2
Induction	Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell. Ref.1
Post-translational modification	Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Translation regulation Transport mRNA transport
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative initiation
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Developmental protein Helicase Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA export from nucleus Inferred from sequence or structural similarity. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from sequence or structural similarity. Source: UniProtKB spermatid development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	chromatoid body Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB ATP-dependent RNA helicase activity Inferred from sequence or structural similarity. Source: UniProtKB RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q9UHL0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHL0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 483

483

ATP-dependent RNA helicase DDX25

PRO_0000030813

Regions

Domain

130 – 300

171

Helicase ATP-binding

Domain

311 – 478

168

Helicase C-terminal

Nucleotide binding

143 – 150

8

ATP By similarity

Motif

61 – 74

14

Nuclear export signal By similarity

Motif

97 – 125

29

Q motif

Motif

100 – 114

15

Nuclear localization signal By similarity

Motif

247 – 250

4

DEAD box

Natural variations

Alternative sequence

1 – 114

114

Missing in isoform 2.

VSP_018875

Experimental info

Sequence conflict

21

1

H → N in AAF21371. Ref.1

Sequence conflict

21

1

H → N in AAU84667. Ref.1

Sequence conflict

381

1

I → T in AAF21371. Ref.1

Sequence conflict

381

1

I → T in AAU84667. Ref.1

Secondary structure

1

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483

Helix Strand Turn

Details...

Beta strand

312 – 318

7

Helix

322 – 333

12

Beta strand

339 – 344

6

Helix

348 – 359

12

Turn

360 – 362

3

Beta strand

365 – 368

4

Helix

374 – 385

12

Beta strand

390 – 394

5

Turn

400 – 402

3

Beta strand

407 – 414

8

Helix

425 – 432

8

Beta strand

442 – 448

7

Helix

450 – 452

3

Helix

453 – 463

11

Beta strand

468 – 470

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 10, 2003. Version 2. Checksum: 32247CDDCA177F48	FASTA	483	54,692
	10 20 30 40 50 60 MASLLWGGDA GAAESERLNS HFSNLSQPRK NLWGIKSTAV RNIDGSINNI NEDDEEDVVD 70 80 90 100 110 120 LAANSLLNKL IHQSLVESSH RVEVLQKDPS SPLYSVKTFE ELRLKEELLK GIYAMGFNRP 130 140 150 160 170 180 SKIQEMALPM MLAHPPQNLI AQSQSGTGKT AAFVLAMLSR VNALELFPQC LCLAPTYELA 190 200 210 220 230 240 LQTGRVVEQM GKFCVDVQVM YAIRGNRIPR GTDITKQIII GTPGTVLDWC FKLKLIDLTK 250 260 270 280 290 300 IRVFVLDEAD VMIDTQGFSD HSIRIQRALP SECQMLLFSA TFEDSVWHFA ERIIPDPNVI 310 320 330 340 350 360 KLRKEELTLN NIRQYYVLCE HRKDKYQALC NIYGSITIGQ AIIFCQTRRN AKWLTVEMIQ 370 380 390 400 410 420 DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR GIDVKQVTIV VNFDLPVKQG 430 440 450 460 470 480 EEPDYETYLH RIGRTGRFGK KGLAFNMIEV DELPSLMKIQ DHFNSSIKQL NAEDMDEIEK IDY
	Isoform 2 [UniParc]. Checksum: B1CB13391FA4B54E	FASTA	369	42,034
	10 20 30 40 50 60 MGFNRPSKIQ EMALPMMLAH PPQNLIAQSQ SGTGKTAAFV LAMLSRVNAL ELFPQCLCLA 70 80 90 100 110 120 PTYELALQTG RVVEQMGKFC VDVQVMYAIR GNRIPRGTDI TKQIIIGTPG TVLDWCFKLK 130 140 150 160 170 180 LIDLTKIRVF VLDEADVMID TQGFSDHSIR IQRALPSECQ MLLFSATFED SVWHFAERII 190 200 210 220 230 240 PDPNVIKLRK EELTLNNIRQ YYVLCEHRKD KYQALCNIYG SITIGQAIIF CQTRRNAKWL 250 260 270 280 290 300 TVEMIQDGHQ VSLLSGELTV EQRASIIQRF RDGKEKVLIT TNVCARGIDV KQVTIVVNFD 310 320 330 340 350 360 LPVKQGEEPD YETYLHRIGR TGRFGKKGLA FNMIEVDELP SLMKIQDHFN SSIKQLNAED MDEIEKIDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel gonadotropin-regulated testicular RNA helicase: a new member of the DEAD-box family." Tang P.-Z., Tsai-Morris C.-H., Dufau M.L. J. Biol. Chem. 274:37932-37940(1999) [PubMed: 10608860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Tissue: Testis.
[2]	Tang P.-Z., Tsai-Morris C.-H., Dufau M.L. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION, TISSUE SPECIFICITY.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Medulla oblongata.
[6]	"Comparative structural analysis of human DEAD-box RNA helicases." Schutz P., Karlberg T., van den Berg S., Collins R., Lehtio L., Hogbom M., Holmberg-Schiavone L., Tempel W., Park H.W., Hammarstrom M., Moche M., Thorsell A.G., Schuler H. PLoS ONE 5:0-0(2010) [PubMed: 20941364] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 307-479.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF155140 mRNA. Translation: AAF21371.2.
AY735312 , AY735301, AY735302, AY735303, AY735304, AY735305, AY735306, AY735307, AY735308, AY735309, AY735310, AY735311 Genomic DNA. Translation: AAU84667.1.
AK312772 mRNA. Translation: BAG35637.1.
CH471065 Genomic DNA. Translation: EAW67669.1.
BC035388 mRNA. Translation: AAH35388.2.
BC050360 mRNA. Translation: AAH50360.2.

IPI

IPI00296139.
IPI00759454.

RefSeq

NP_037396.3. NM_013264.3.

UniGene

Hs.420263.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RB4	X-ray	2.80	A/B	307-479	[»]

ProteinModelPortal

Q9UHL0.

SMR

Q9UHL0. Positions 80-476.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9UHL0.

PTM databases

PhosphoSite

Q9UHL0.

Polymorphism databases

DMDM

61222937.

Proteomic databases

PRIDE

Q9UHL0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263576; ENSP00000263576; ENSG00000109832.

GeneID

29118.

KEGG

hsa:29118.

UCSC

uc001qcz.2. human.

Organism-specific databases

CTD

29118.

GeneCards

GC11P125806.

H-InvDB

HIX0201693.

HGNC

HGNC:18698. DDX25.

HPA

HPA020090.
HPA020137.

MIM

607663. gene.

neXtProt

NX_Q9UHL0.

PharmGKB

PA38644.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16877.

GeneTree

ENSGT00530000063236.

HOGENOM

HBG737336.

HOVERGEN

HBG107989.

InParanoid

Q9UHL0.

OMA

RGNRIPR.

OrthoDB

EOG4NS3BD.

PhylomeDB

Q9UHL0.

Gene expression databases

ArrayExpress

Q9UHL0.

Bgee

Q9UHL0.

CleanEx

HS_DDX25.

Genevestigator

Q9UHL0.

GermOnline

ENSG00000109832. Homo sapiens.

Family and domain databases

InterPro

IPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001650. Helicase_C.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

PROSITE

PS00039. DEAD_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

52207.

SOURCE

Search...

Entry information

Entry name

DDX25_HUMAN

Accession

Primary (citable) accession number: Q9UHL0
Secondary accession number(s): B2R6Z0 , Q5XVN2, Q86W81, Q8IYP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	October 10, 2003
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.