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Protein

ATP-dependent RNA helicase DDX25

Gene

DDX25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 1508ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.4.13. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:DDX25
Synonyms:GRTH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:18698. DDX25.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus By similarity

  • Note: Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38644.

Polymorphism and mutation databases

BioMutaiDDX25.
DMDMi61222937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483ATP-dependent RNA helicase DDX25PRO_0000030813Add
BLAST

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UHL0.
MaxQBiQ9UHL0.
PaxDbiQ9UHL0.
PRIDEiQ9UHL0.

PTM databases

PhosphoSiteiQ9UHL0.

Expressioni

Tissue specificityi

Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus.2 Publications

Inductioni

Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell.1 Publication

Gene expression databases

BgeeiQ9UHL0.
CleanExiHS_DDX25.
ExpressionAtlasiQ9UHL0. baseline and differential.
GenevisibleiQ9UHL0. HS.

Organism-specific databases

HPAiHPA020090.
HPA020137.

Interactioni

Protein-protein interaction databases

BioGridi118884. 1 interaction.
STRINGi9606.ENSP00000263576.

Structurei

Secondary structure

1
483
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi312 – 3187Combined sources
Helixi322 – 33312Combined sources
Beta strandi339 – 3446Combined sources
Helixi348 – 35912Combined sources
Turni360 – 3623Combined sources
Beta strandi365 – 3684Combined sources
Helixi374 – 38512Combined sources
Beta strandi390 – 3945Combined sources
Turni400 – 4023Combined sources
Beta strandi407 – 4148Combined sources
Helixi425 – 4328Combined sources
Beta strandi442 – 4487Combined sources
Helixi450 – 4523Combined sources
Helixi453 – 46311Combined sources
Beta strandi468 – 4703Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RB4X-ray2.80A/B307-479[»]
ProteinModelPortaliQ9UHL0.
SMRiQ9UHL0. Positions 80-476.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UHL0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 300171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini311 – 478168Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7414Nuclear export signalBy similarityAdd
BLAST
Motifi97 – 12529Q motifAdd
BLAST
Motifi100 – 11415Nuclear localization signalBy similarityAdd
BLAST
Motifi247 – 2504DEAD box

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9UHL0.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG7TMZRJ.
PhylomeDBiQ9UHL0.
TreeFamiTF314957.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9UHL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLSQPRK NLWGIKSTAV RNIDGSINNI
60 70 80 90 100
NEDDEEDVVD LAANSLLNKL IHQSLVESSH RVEVLQKDPS SPLYSVKTFE
110 120 130 140 150
ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT
160 170 180 190 200
AAFVLAMLSR VNALELFPQC LCLAPTYELA LQTGRVVEQM GKFCVDVQVM
210 220 230 240 250
YAIRGNRIPR GTDITKQIII GTPGTVLDWC FKLKLIDLTK IRVFVLDEAD
260 270 280 290 300
VMIDTQGFSD HSIRIQRALP SECQMLLFSA TFEDSVWHFA ERIIPDPNVI
310 320 330 340 350
KLRKEELTLN NIRQYYVLCE HRKDKYQALC NIYGSITIGQ AIIFCQTRRN
360 370 380 390 400
AKWLTVEMIQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR
410 420 430 440 450
GIDVKQVTIV VNFDLPVKQG EEPDYETYLH RIGRTGRFGK KGLAFNMIEV
460 470 480
DELPSLMKIQ DHFNSSIKQL NAEDMDEIEK IDY
Length:483
Mass (Da):54,692
Last modified:October 10, 2003 - v2
Checksum:i32247CDDCA177F48
GO
Isoform 2 (identifier: Q9UHL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Show »
Length:369
Mass (Da):42,034
Checksum:iB1CB13391FA4B54E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211H → N in AAF21371 (PubMed:10608860).Curated
Sequence conflicti21 – 211H → N in AAU84667 (PubMed:10608860).Curated
Sequence conflicti381 – 3811I → T in AAF21371 (PubMed:10608860).Curated
Sequence conflicti381 – 3811I → T in AAU84667 (PubMed:10608860).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 114114Missing in isoform 2. 2 PublicationsVSP_018875Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155140 mRNA. Translation: AAF21371.2.
AY735312
, AY735301, AY735302, AY735303, AY735304, AY735305, AY735306, AY735307, AY735308, AY735309, AY735310, AY735311 Genomic DNA. Translation: AAU84667.1.
AK312772 mRNA. Translation: BAG35637.1.
CH471065 Genomic DNA. Translation: EAW67669.1.
BC035388 mRNA. Translation: AAH35388.2.
BC050360 mRNA. Translation: AAH50360.2.
CCDSiCCDS44766.1. [Q9UHL0-1]
RefSeqiNP_037396.3. NM_013264.4. [Q9UHL0-1]
UniGeneiHs.420263.

Genome annotation databases

EnsembliENST00000263576; ENSP00000263576; ENSG00000109832. [Q9UHL0-1]
GeneIDi29118.
KEGGihsa:29118.
UCSCiuc001qcz.6. human. [Q9UHL0-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155140 mRNA. Translation: AAF21371.2.
AY735312
, AY735301, AY735302, AY735303, AY735304, AY735305, AY735306, AY735307, AY735308, AY735309, AY735310, AY735311 Genomic DNA. Translation: AAU84667.1.
AK312772 mRNA. Translation: BAG35637.1.
CH471065 Genomic DNA. Translation: EAW67669.1.
BC035388 mRNA. Translation: AAH35388.2.
BC050360 mRNA. Translation: AAH50360.2.
CCDSiCCDS44766.1. [Q9UHL0-1]
RefSeqiNP_037396.3. NM_013264.4. [Q9UHL0-1]
UniGeneiHs.420263.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RB4X-ray2.80A/B307-479[»]
ProteinModelPortaliQ9UHL0.
SMRiQ9UHL0. Positions 80-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118884. 1 interaction.
STRINGi9606.ENSP00000263576.

PTM databases

PhosphoSiteiQ9UHL0.

Polymorphism and mutation databases

BioMutaiDDX25.
DMDMi61222937.

Proteomic databases

EPDiQ9UHL0.
MaxQBiQ9UHL0.
PaxDbiQ9UHL0.
PRIDEiQ9UHL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263576; ENSP00000263576; ENSG00000109832. [Q9UHL0-1]
GeneIDi29118.
KEGGihsa:29118.
UCSCiuc001qcz.6. human. [Q9UHL0-1]

Organism-specific databases

CTDi29118.
GeneCardsiDDX25.
HGNCiHGNC:18698. DDX25.
HPAiHPA020090.
HPA020137.
MIMi607663. gene.
neXtProtiNX_Q9UHL0.
PharmGKBiPA38644.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9UHL0.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG7TMZRJ.
PhylomeDBiQ9UHL0.
TreeFamiTF314957.

Enzyme and pathway databases

BRENDAi3.6.4.13. 2681.

Miscellaneous databases

ChiTaRSiDDX25. human.
EvolutionaryTraceiQ9UHL0.
GenomeRNAii29118.
NextBioi52207.
PROiQ9UHL0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UHL0.
CleanExiHS_DDX25.
ExpressionAtlasiQ9UHL0. baseline and differential.
GenevisibleiQ9UHL0. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel gonadotropin-regulated testicular RNA helicase: a new member of the DEAD-box family."
    Tang P.-Z., Tsai-Morris C.-H., Dufau M.L.
    J. Biol. Chem. 274:37932-37940(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Testis.
  2. Tang P.-Z., Tsai-Morris C.-H., Dufau M.L.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION, TISSUE SPECIFICITY.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Medulla oblongata.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 307-479.

Entry informationi

Entry nameiDDX25_HUMAN
AccessioniPrimary (citable) accession number: Q9UHL0
Secondary accession number(s): B2R6Z0
, Q5XVN2, Q86W81, Q8IYP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: May 11, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.