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Protein

ATP-dependent RNA helicase DDX25

Gene

DDX25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 150ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000109832-MONOMER.
BRENDAi3.6.4.13. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:DDX25
Synonyms:GRTH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:18698. DDX25.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus By similarity

  • Note: Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi29118.
OpenTargetsiENSG00000109832.
PharmGKBiPA38644.

Polymorphism and mutation databases

BioMutaiDDX25.
DMDMi61222937.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000308131 – 483ATP-dependent RNA helicase DDX25Add BLAST483

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UHL0.
PaxDbiQ9UHL0.
PeptideAtlasiQ9UHL0.
PRIDEiQ9UHL0.

PTM databases

iPTMnetiQ9UHL0.
PhosphoSitePlusiQ9UHL0.

Expressioni

Tissue specificityi

Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus.2 Publications

Inductioni

Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell.1 Publication

Gene expression databases

BgeeiENSG00000109832.
CleanExiHS_DDX25.
ExpressionAtlasiQ9UHL0. baseline and differential.
GenevisibleiQ9UHL0. HS.

Organism-specific databases

HPAiHPA020090.
HPA020137.

Interactioni

Protein-protein interaction databases

BioGridi118884. 1 interactor.
IntActiQ9UHL0. 1 interactor.
STRINGi9606.ENSP00000263576.

Structurei

Secondary structure

1483
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi312 – 318Combined sources7
Helixi322 – 333Combined sources12
Beta strandi339 – 344Combined sources6
Helixi348 – 359Combined sources12
Turni360 – 362Combined sources3
Beta strandi365 – 368Combined sources4
Helixi374 – 385Combined sources12
Beta strandi390 – 394Combined sources5
Turni400 – 402Combined sources3
Beta strandi407 – 414Combined sources8
Helixi425 – 432Combined sources8
Beta strandi442 – 448Combined sources7
Helixi450 – 452Combined sources3
Helixi453 – 463Combined sources11
Beta strandi468 – 470Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RB4X-ray2.80A/B307-479[»]
ProteinModelPortaliQ9UHL0.
SMRiQ9UHL0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UHL0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 300Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini311 – 478Helicase C-terminalPROSITE-ProRule annotationAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 74Nuclear export signalBy similarityAdd BLAST14
Motifi97 – 125Q motifAdd BLAST29
Motifi100 – 114Nuclear localization signalBy similarityAdd BLAST15
Motifi247 – 250DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9UHL0.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG091G03PS.
PhylomeDBiQ9UHL0.
TreeFamiTF314957.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9UHL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLSQPRK NLWGIKSTAV RNIDGSINNI
60 70 80 90 100
NEDDEEDVVD LAANSLLNKL IHQSLVESSH RVEVLQKDPS SPLYSVKTFE
110 120 130 140 150
ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT
160 170 180 190 200
AAFVLAMLSR VNALELFPQC LCLAPTYELA LQTGRVVEQM GKFCVDVQVM
210 220 230 240 250
YAIRGNRIPR GTDITKQIII GTPGTVLDWC FKLKLIDLTK IRVFVLDEAD
260 270 280 290 300
VMIDTQGFSD HSIRIQRALP SECQMLLFSA TFEDSVWHFA ERIIPDPNVI
310 320 330 340 350
KLRKEELTLN NIRQYYVLCE HRKDKYQALC NIYGSITIGQ AIIFCQTRRN
360 370 380 390 400
AKWLTVEMIQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR
410 420 430 440 450
GIDVKQVTIV VNFDLPVKQG EEPDYETYLH RIGRTGRFGK KGLAFNMIEV
460 470 480
DELPSLMKIQ DHFNSSIKQL NAEDMDEIEK IDY
Length:483
Mass (Da):54,692
Last modified:October 10, 2003 - v2
Checksum:i32247CDDCA177F48
GO
Isoform 2 (identifier: Q9UHL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Show »
Length:369
Mass (Da):42,034
Checksum:iB1CB13391FA4B54E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21H → N in AAF21371 (PubMed:10608860).Curated1
Sequence conflicti21H → N in AAU84667 (PubMed:10608860).Curated1
Sequence conflicti381I → T in AAF21371 (PubMed:10608860).Curated1
Sequence conflicti381I → T in AAU84667 (PubMed:10608860).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188751 – 114Missing in isoform 2. 2 PublicationsAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155140 mRNA. Translation: AAF21371.2.
AY735312
, AY735301, AY735302, AY735303, AY735304, AY735305, AY735306, AY735307, AY735308, AY735309, AY735310, AY735311 Genomic DNA. Translation: AAU84667.1.
AK312772 mRNA. Translation: BAG35637.1.
CH471065 Genomic DNA. Translation: EAW67669.1.
BC035388 mRNA. Translation: AAH35388.2.
BC050360 mRNA. Translation: AAH50360.2.
CCDSiCCDS44766.1. [Q9UHL0-1]
CCDS81646.1. [Q9UHL0-2]
RefSeqiNP_001317367.1. NM_001330438.1.
NP_037396.3. NM_013264.4. [Q9UHL0-1]
UniGeneiHs.420263.

Genome annotation databases

EnsembliENST00000263576; ENSP00000263576; ENSG00000109832. [Q9UHL0-1]
ENST00000525943; ENSP00000490224; ENSG00000109832. [Q9UHL0-2]
GeneIDi29118.
KEGGihsa:29118.
UCSCiuc001qcz.6. human. [Q9UHL0-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155140 mRNA. Translation: AAF21371.2.
AY735312
, AY735301, AY735302, AY735303, AY735304, AY735305, AY735306, AY735307, AY735308, AY735309, AY735310, AY735311 Genomic DNA. Translation: AAU84667.1.
AK312772 mRNA. Translation: BAG35637.1.
CH471065 Genomic DNA. Translation: EAW67669.1.
BC035388 mRNA. Translation: AAH35388.2.
BC050360 mRNA. Translation: AAH50360.2.
CCDSiCCDS44766.1. [Q9UHL0-1]
CCDS81646.1. [Q9UHL0-2]
RefSeqiNP_001317367.1. NM_001330438.1.
NP_037396.3. NM_013264.4. [Q9UHL0-1]
UniGeneiHs.420263.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RB4X-ray2.80A/B307-479[»]
ProteinModelPortaliQ9UHL0.
SMRiQ9UHL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118884. 1 interactor.
IntActiQ9UHL0. 1 interactor.
STRINGi9606.ENSP00000263576.

PTM databases

iPTMnetiQ9UHL0.
PhosphoSitePlusiQ9UHL0.

Polymorphism and mutation databases

BioMutaiDDX25.
DMDMi61222937.

Proteomic databases

EPDiQ9UHL0.
PaxDbiQ9UHL0.
PeptideAtlasiQ9UHL0.
PRIDEiQ9UHL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263576; ENSP00000263576; ENSG00000109832. [Q9UHL0-1]
ENST00000525943; ENSP00000490224; ENSG00000109832. [Q9UHL0-2]
GeneIDi29118.
KEGGihsa:29118.
UCSCiuc001qcz.6. human. [Q9UHL0-1]

Organism-specific databases

CTDi29118.
DisGeNETi29118.
GeneCardsiDDX25.
HGNCiHGNC:18698. DDX25.
HPAiHPA020090.
HPA020137.
MIMi607663. gene.
neXtProtiNX_Q9UHL0.
OpenTargetsiENSG00000109832.
PharmGKBiPA38644.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9UHL0.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG091G03PS.
PhylomeDBiQ9UHL0.
TreeFamiTF314957.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000109832-MONOMER.
BRENDAi3.6.4.13. 2681.

Miscellaneous databases

ChiTaRSiDDX25. human.
EvolutionaryTraceiQ9UHL0.
GenomeRNAii29118.
PROiQ9UHL0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109832.
CleanExiHS_DDX25.
ExpressionAtlasiQ9UHL0. baseline and differential.
GenevisibleiQ9UHL0. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX25_HUMAN
AccessioniPrimary (citable) accession number: Q9UHL0
Secondary accession number(s): B2R6Z0
, Q5XVN2, Q86W81, Q8IYP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.