Reviewed, UniProtKB/Swiss-Prot Q9UHJ9 (PGAP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Post-GPI attachment to proteins factor 2
Alternative name(s):
    FGF receptor-activating protein 1

Gene names

Name:	PGAP2
Synonyms:	FRAG1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the lipid remodeling steps of GPI-anchor maturation. Required for stable expression of GPI-anchored proteins at the cell surface By similarity.
Subunit structure	Interacts with PGAP2IP By similarity.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Ubiquitously expressed, with highest levels in testis and pancreas. Ref.1
Sequence similarities	Belongs to the PGAP2 family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein transporter activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UHJ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHJ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     56-116: Missing.

Isoform 3 (identifier: Q9UHJ9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     56-116: Missing.
     233-236: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Post-GPI attachment to proteins factor 2		PRO_0000326094
Regions
	Transmembrane	24 – 44	21	Potential
	Transmembrane	85 – 105	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	205 – 225	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	271 – 291	21	Potential
Natural variations
	Alternative sequence	56 – 116	61	Missing in isoform 2 and isoform 3.		VSP_032550
	Alternative sequence	233 – 236	4	Missing in isoform 3.		VSP_032551
Experimental info
	Sequence conflict	134	1	Q → H in AAF19156. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 64F89733A1F6270E	FASTA	315	36,417
	10 20 30 40 50 60 MYQVPLPLDR DGTLVRLRFT MVALVTVCCP LVAFLFCILW SLLFHFKETT ATHCGATPCR 70 80 90 100 110 120 MFSAASQPLD PDGTLFRLRF TAMVWWAITF PVFGFFFCII WSLVFHFEYT VATDCGVPNY 130 140 150 160 170 180 LPSVSSAIGG EVPQRYVWRF CIGLHSAPRF LVAFAYWNHY LSCTSPCSCY RPLCRLNFGL 190 200 210 220 230 240 NVVENLALLV LTYVSSSEDF TIHENAFIVF IASSLGHMLL TCILWRLTKK HTVSQEDRKS 250 260 270 280 290 300 YSWKQRLFII NFISFFSALA VYFRHNMYCE AGVYTIFAIL EYTVVLTNMA FHMTAWWDFG 310 NKELLITSQP EEKRF
	Isoform 2. Checksum: 2FB502D505DCCF64	FASTA	254	29,400
	10 20 30 40 50 60 MYQVPLPLDR DGTLVRLRFT MVALVTVCCP LVAFLFCILW SLLFHFKETT ATHCGVPNYL 70 80 90 100 110 120 PSVSSAIGGE VPQRYVWRFC IGLHSAPRFL VAFAYWNHYL SCTSPCSCYR PLCRLNFGLN 130 140 150 160 170 180 VVENLALLVL TYVSSSEDFT IHENAFIVFI ASSLGHMLLT CILWRLTKKH TVSQEDRKSY 190 200 210 220 230 240 SWKQRLFIIN FISFFSALAV YFRHNMYCEA GVYTIFAILE YTVVLTNMAF HMTAWWDFGN 250 KELLITSQPE EKRF
	Isoform 3. Checksum: 6B8A396B2A362942	FASTA	250	28,957
	10 20 30 40 50 60 MYQVPLPLDR DGTLVRLRFT MVALVTVCCP LVAFLFCILW SLLFHFKETT ATHCGVPNYL 70 80 90 100 110 120 PSVSSAIGGE VPQRYVWRFC IGLHSAPRFL VAFAYWNHYL SCTSPCSCYR PLCRLNFGLN 130 140 150 160 170 180 VVENLALLVL TYVSSSEDFT IHENAFIVFI ASSLGHMLLT CILWRLTKKH TDRKSYSWKQ 190 200 210 220 230 240 RLFIINFISF FSALAVYFRH NMYCEAGVYT IFAILEYTVV LTNMAFHMTA WWDFGNKELL 250 ITSQPEEKRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human FRAG1 encodes a novel membrane-spanning protein that localizes to chromosome 11p15.5, a region of frequent loss of heterozygosity in cancer." Lorenzi M.V., Castagnino P., Aaronson D.C., Lieb D.C., Lee C.C., Keck C.L., Popescu N.C., Miki T. Genomics 62:59-66(1999) [PubMed: 10585768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	Zhou G., Yu R., Zhong G., Li H., Shen C., Zheng G., Ke R., Lin L., Yang S. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Kidney.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-55. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF159615 mRNA. Translation: AAF12755.1. AY373030 mRNA. Translation: AAQ75733.1. AF159621 , AF159616, AF159617, AF159618, AF159619, AF159620 Genomic DNA. Translation: AAF19156.1. AK292181 mRNA. Translation: BAF84870.1. CH471158 Genomic DNA. Translation: EAX02569.1. CH471158 Genomic DNA. Translation: EAX02568.1. CH471158 Genomic DNA. Translation: EAX02571.1. BC009930 mRNA. Translation: AAH09930.1. AL096753 mRNA. Translation: CAB46428.3.
IPI	IPI00062297. IPI00383797. IPI00888091.
PIR	T12505.
RefSeq	NP_001138910.1. NP_055304.1.
UniGene	Hs.133968
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000148985. Homo sapiens. [Contig view]
GeneID	27315.
KEGG	hsa:27315.
Organism-specific databases
GeneCards	GC11P003775.
Phylogenomic databases
HOVERGEN	Q9UHJ9.
OMA	Q9UHJ9. THCGVPN.
Gene expression databases
Bgee	Q9UHJ9.
Family and domain databases
InterPro	IPR019402. Frag1/DRAM/Sfk1. [Graphical view]
Pfam	PF10277. Frag1. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	50319.

Entry information

Entry name

PGAP2_HUMAN

Accession

Primary (citable) accession number: Q9UHJ9
Secondary accession number(s): Q6UC77 , Q96G66, Q9UF01, Q9Y4N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)