Q9UHI7 (S23A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 23 member 1

Alternative name(s):
Na(+)/L-ascorbic acid transporter 1
Sodium-dependent vitamin C transporter 1
Short name=hSVCT1
Yolk sac permease-like molecule 3

Gene names

Name:	SLC23A1
Synonyms:	SVCT1, YSPL3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each ascorbate.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Highly expressed in adult small intestine, kidney, thymus, ovary, colon, prostate and liver, and in fetal kidney, liver and thymus.
Post-translational modification	Phosphorylated Probable.
Miscellaneous	Treatment with the protein kinase C stimulator PMA results in a 10-fold decrease in ascorbate accumulation in transfected cells.
Sequence similarities	Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from sequence or structural similarity. Source: UniProtKB nucleobase-containing compound metabolic process Traceable author statement. Source: ProtInc response to toxin Inferred from direct assay. Source: UniProtKB transepithelial L-ascorbic acid transport Inferred from direct assay. Source: UniProtKB water-soluble vitamin metabolic process Traceable author statement. Source: Reactome
Cellular component	apical plasma membrane Inferred from direct assay. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Traceable author statement. Source: ProtInc intracellular organelle Inferred from direct assay. Source: UniProtKB membrane fraction Traceable author statement. Source: ProtInc
Molecular function	L-ascorbate:sodium symporter activity Inferred from direct assay. Source: UniProtKB dehydroascorbic acid transporter activity Inferred from mutant phenotype. Source: UniProtKB nucleobase transmembrane transporter activity Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct sodium-dependent L-ascorbate transmembrane transporter activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCK1	P16333	2	EBI-1759386,EBI-389883

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q9UHI7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHI7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     156-156: V → VGLHV

Note: Inactive.

Isoform 3 (identifier: Q9UHI7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     92-430: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Solute carrier family 23 member 1		PRO_0000165975
Regions
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Transmembrane	104 – 124	21	Helical; Potential
	Transmembrane	160 – 180	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	359 – 379	21	Helical; Potential
	Transmembrane	403 – 423	21	Helical; Potential
	Transmembrane	427 – 447	21	Helical; Potential
	Transmembrane	458 – 478	21	Helical; Potential
	Transmembrane	491 – 511	21	Helical; Potential
Amino acid modifications
	Modified residue	596	1	Phosphothreonine By similarity
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	92 – 430	339	Missing in isoform 3.		VSP_006813
	Alternative sequence	156	1	V → VGLHV in isoform 2.		VSP_006814
	Natural variant	218	1	I → V. Corresponds to variant rs34521685 [ dbSNP | Ensembl ].		VAR_053451
	Natural variant	258	1	M → V. Corresponds to variant rs35817838 [ dbSNP | Ensembl ].		VAR_053452
	Natural variant	264	1	V → M. Corresponds to variant rs33972313 [ dbSNP | Ensembl ].		VAR_053453
	Natural variant	421	1	S → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8 Corresponds to variant rs6596474 [ dbSNP | Ensembl ].		VAR_062111
Experimental info
	Sequence conflict	11	1	T → A in AAF24759. Ref.2
	Sequence conflict	52 – 57	6	YLTCFS → IHDCLR in AAC78804. Ref.1
	Sequence conflict	75 – 80	6	DQHMVS → SQTLHC in AAC78804. Ref.1
	Sequence conflict	139	1	W → S in AAC78804. Ref.1
	Sequence conflict	153	1	I → N in AAC78804. Ref.1
	Sequence conflict	155	1	E → D in AAF22490. Ref.2
	Sequence conflict	182 – 183	2	YI → SL in AAC78804. Ref.1
	Sequence conflict	207	1	A → P in AAC78804. Ref.1
	Sequence conflict	269	1	Y → I in AAC78804. Ref.1
	Sequence conflict	275	1	D → E in AAF22490. Ref.2
	Sequence conflict	284	1	Y → I in AAC78804. Ref.1
	Sequence conflict	434	1	T → S in AAC78804. Ref.1
	Sequence conflict	451 – 452	2	DM → AL in AAC78804. Ref.1
	Sequence conflict	476 – 477	2	ES → SP in AAF22490. Ref.2
	Sequence conflict	548	1	I → F in AAC78804. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 3. Checksum: 1191B2B43CE76FD6	FASTA	598	64,831
	10 20 30 40 50 60 MRAQEDLEGR TQHETTRDPS TPLPTEPKFD MLYKIEDVPP WYLCILLGFQ HYLTCFSGTI 70 80 90 100 110 120 AVPFLLAEAL CVGHDQHMVS QLIGTIFTCV GITTLIQTTV GIRLPLFQAS AFAFLVPAKA 130 140 150 160 170 180 ILALERWKCP PEEEIYGNWS LPLNTSHIWH PRIREVQGAI MVSSVVEVVI GLLGLPGALL 190 200 210 220 230 240 NYIGPLTVTP TVSLIGLSVF QAAGDRAGSH WGISACSILL IILFSQYLRN LTFLLPVYRW 250 260 270 280 290 300 GKGLTLLRIQ IFKMFPIMLA IMTVWLLCYV LTLTDVLPTD PKAYGFQART DARGDIMAIA 310 320 330 340 350 360 PWIRIPYPCQ WGLPTVTAAA VLGMFSATLA GIIESIGDYY ACARLAGAPP PPVHAINRGI 370 380 390 400 410 420 FTEGICCIIA GLLGTGNGST SSSPNIGVLG ITKVGSRRVV QYGAAIMLVL GTIGKFTALF 430 440 450 460 470 480 SSLPDPILGG MFCTLFGMIT AVGLSNLQFV DMNSSRNLFV LGFSMFFGLT LPNYLESNPG 490 500 510 520 530 540 AINTGILEVD QILIVLLTTE MFVGGCLAFI LDNTVPGSPE ERGLIQWKAG AHANSDMSSS 550 560 570 580 590 LKSYDFPIGM GIVKRITFLK YIPICPVFKG FSSSSKDQIA IPEDTPENTE TASVCTKV
	Isoform 2 [UniParc]. Checksum: 0D03E3405E2E1EB3	FASTA	602	65,238
	10 20 30 40 50 60 MRAQEDLEGR TQHETTRDPS TPLPTEPKFD MLYKIEDVPP WYLCILLGFQ HYLTCFSGTI 70 80 90 100 110 120 AVPFLLAEAL CVGHDQHMVS QLIGTIFTCV GITTLIQTTV GIRLPLFQAS AFAFLVPAKA 130 140 150 160 170 180 ILALERWKCP PEEEIYGNWS LPLNTSHIWH PRIREVGLHV QGAIMVSSVV EVVIGLLGLP 190 200 210 220 230 240 GALLNYIGPL TVTPTVSLIG LSVFQAAGDR AGSHWGISAC SILLIILFSQ YLRNLTFLLP 250 260 270 280 290 300 VYRWGKGLTL LRIQIFKMFP IMLAIMTVWL LCYVLTLTDV LPTDPKAYGF QARTDARGDI 310 320 330 340 350 360 MAIAPWIRIP YPCQWGLPTV TAAAVLGMFS ATLAGIIESI GDYYACARLA GAPPPPVHAI 370 380 390 400 410 420 NRGIFTEGIC CIIAGLLGTG NGSTSSSPNI GVLGITKVGS RRVVQYGAAI MLVLGTIGKF 430 440 450 460 470 480 TALFSSLPDP ILGGMFCTLF GMITAVGLSN LQFVDMNSSR NLFVLGFSMF FGLTLPNYLE 490 500 510 520 530 540 SNPGAINTGI LEVDQILIVL LTTEMFVGGC LAFILDNTVP GSPEERGLIQ WKAGAHANSD 550 560 570 580 590 600 MSSSLKSYDF PIGMGIVKRI TFLKYIPICP VFKGFSSSSK DQIAIPEDTP ENTETASVCT KV
	Isoform 3 [UniParc]. Checksum: 1F422EEA2AE6A2C5	FASTA	259	28,531
	10 20 30 40 50 60 MRAQEDLEGR TQHETTRDPS TPLPTEPKFD MLYKIEDVPP WYLCILLGFQ HYLTCFSGTI 70 80 90 100 110 120 AVPFLLAEAL CVGHDQHMVS QLIGTIFTCV GMFCTLFGMI TAVGLSNLQF VDMNSSRNLF 130 140 150 160 170 180 VLGFSMFFGL TLPNYLESNP GAINTGILEV DQILIVLLTT EMFVGGCLAF ILDNTVPGSP 190 200 210 220 230 240 EERGLIQWKA GAHANSDMSS SLKSYDFPIG MGIVKRITFL KYIPICPVFK GFSSSSKDQI 250 AIPEDTPENT ETASVCTKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters." Faaland C.A., Race J.E., Ricken G., Warner F.J., Williams W.J., Holtzman E.J. Biochim. Biophys. Acta 1442:353-360(1998) [PubMed: 9804989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421. Tissue: Fetal liver and Kidney.
[2]	"Human Na(+)-dependent vitamin C transporter 1 (hSVCT1): primary structure, functional characteristics and evidence for a non-functional splice variant." Wang H., Dutta B., Huang W., Devoe L.D., Leibach F.H., Ganapathy V., Prasad P.D. Biochim. Biophys. Acta 1461:1-9(1999) [PubMed: 10556483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-421. Tissue: Intestinal epithelium.
[3]	"Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2." Daruwala R.C., Song J., Koh W.S., Rumsey S.C., Levine M. FEBS Lett. 460:480-484(1999) [PubMed: 10556521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421. Tissue: Kidney.
[4]	"Human vitamin C (L-ascorbic acid) transporter SVCT1." Wang Y., Mackenzie B., Tsukaguchi H., Weremowicz S., Morton C.C., Hediger M.A. Biochem. Biophys. Res. Commun. 267:488-494(2000) [PubMed: 10631088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421. Tissue: Kidney.
[5]	"Characterization of the genomic structure of the human vitamin C transporter SVCT1 (SLC23A2)." Erichsen H.C., Eck P., Levine M., Chanock S. J. Nutr. 131:2623-2627(2001) [PubMed: 11584081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), VARIANT ALA-421.
[6]	"Vitamin C transport systems of mammalian cells." Liang W.J., Johnson D., Jarvis S.M. Mol. Membr. Biol. 18:87-95(2001) [PubMed: 11396616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421. Tissue: Liver cancer.
[7]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT ALA-421. Tissue: Colon and Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058317 mRNA. Translation: AAC78804.1. AF170911 mRNA. Translation: AAF24759.1. AJ269477 mRNA. Translation: CAB58119.1. AF098277 mRNA. Translation: AAF22490.1. AF375875 Genomic DNA. Translation: AAK97398.1. AJ250807 mRNA. Translation: CAC15384.1. AC135457 Genomic DNA. No translation available. BC019225 mRNA. Translation: AAH19225.1. BC050261 mRNA. Translation: AAH50261.1.
IPI	IPI00220368. IPI00220369. IPI00303368.
PIR	JC7182.
RefSeq	NP_005838.3. NM_005847.4. NP_689898.2. NM_152685.3.
UniGene	Hs.643467.
3D structure databases
ProteinModelPortal	Q9UHI7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UHI7. 4 interactions.
MINT	MINT-6945553.
STRING	Q9UHI7.
PTM databases
PhosphoSite	Q9UHI7.
Polymorphism databases
DMDM	296452969.
Proteomic databases
PRIDE	Q9UHI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000348729; ENSP00000302701; ENSG00000170482.
GeneID	9963.
KEGG	hsa:9963.
UCSC	uc003leg.1. human. uc003leh.1. human.
Organism-specific databases
CTD	9963.
GeneCards	GC05M138702.
HGNC	HGNC:10974. SLC23A1.
MIM	603790. gene.
neXtProt	NX_Q9UHI7.
PharmGKB	PA35850.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14278.
GeneTree	ENSGT00390000015686.
HOVERGEN	HBG056256.
OMA	PKAYGFQ.
OrthoDB	EOG4C2H99.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9UHI7.
Bgee	Q9UHI7.
CleanEx	HS_SLC23A1.
Genevestigator	Q9UHI7.
GermOnline	ENSG00000170482. Homo sapiens.
Family and domain databases
InterPro	IPR006043. Xant/urac/vitC. [Graphical view]
KO	K14611.
PANTHER	PTHR11119. Xant/urac/vitC. 1 hit.
Pfam	PF00860. Xan_ur_permease. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00126. Vitamin C.
NextBio	37598.
SOURCE	Search...

Entry information

Entry name

S23A1_HUMAN

Accession

Primary (citable) accession number: Q9UHI7
Secondary accession number(s): O95191 , Q8WWB6, Q9UGH4, Q9UI39

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.