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Q9UHI7 (S23A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 23 member 1
Alternative name(s):
Na(+)/L-ascorbic acid transporter 1
Sodium-dependent vitamin C transporter 1
Short name=hSVCT1
Yolk sac permease-like molecule 3
Gene names
Name:SLC23A1
Synonyms:SVCT1, YSPL3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each ascorbate.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.9.

Tissue specificity

Highly expressed in adult small intestine, kidney, thymus, ovary, colon, prostate and liver, and in fetal kidney, liver and thymus.

Post-translational modification

Phosphorylated Probable.

Miscellaneous

Treatment with the protein kinase C stimulator PMA results in a 10-fold decrease in ascorbate accumulation in transfected cells.

Sequence similarities

Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Sodium transport
Symport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-ascorbic acid metabolic process

Traceable author statement. Source: Reactome

L-ascorbic acid transport

Inferred from direct assay Ref.4PubMed 11895172PubMed 18247577PubMed 18417304PubMed 19216494. Source: UniProtKB

brain development

Inferred from sequence or structural similarity. Source: UniProtKB

dehydroascorbic acid transport

Inferred from mutant phenotype PubMed 18247577. Source: UniProtKB

lung development

Inferred from electronic annotation. Source: Ensembl

nucleobase transport

Traceable author statement PubMed 10395795. Source: ProtInc

nucleobase-containing compound metabolic process

Traceable author statement Ref.1. Source: ProtInc

response to toxic substance

Inferred from direct assay PubMed 17575980. Source: UniProtKB

small molecule metabolic process

Traceable author statement. Source: Reactome

sodium ion transmembrane transport

Inferred from direct assay PubMed 18094143. Source: GOC

sodium ion transport

Inferred from direct assay PubMed 18094143. Source: UniProtKB

transepithelial L-ascorbic acid transport

Inferred from direct assay PubMed 18417304. Source: UniProtKB

vitamin metabolic process

Traceable author statement. Source: Reactome

vitamin transmembrane transport

Inferred from direct assay Ref.4PubMed 11895172PubMed 15993839PubMed 18094143. Source: GOC

water-soluble vitamin metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 15993839PubMed 18094143PubMed 18247577PubMed 18417304PubMed 19216494. Source: UniProtKB

basal plasma membrane

Inferred from electronic annotation. Source: Ensembl

brush border

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

integral component of plasma membrane

Traceable author statement PubMed 10395795. Source: ProtInc

intracellular organelle

Inferred from direct assay PubMed 15993839. Source: UniProtKB

membrane

Traceable author statement Ref.1. Source: ProtInc

plasma membrane

Inferred from direct assay Ref.4. Source: UniProtKB

   Molecular_functionL-ascorbate:sodium symporter activity

Inferred from direct assay PubMed 18094143. Source: UniProtKB

L-ascorbic acid transporter activity

Inferred from direct assay Ref.4PubMed 11895172PubMed 18247577PubMed 18417304PubMed 19216494. Source: UniProtKB

dehydroascorbic acid transporter activity

Inferred from mutant phenotype PubMed 18247577. Source: UniProtKB

nucleobase transmembrane transporter activity

Traceable author statement PubMed 10395795. Source: ProtInc

sodium ion transmembrane transporter activity

Inferred from direct assay PubMed 18094143. Source: UniProtKB

sodium-dependent L-ascorbate transmembrane transporter activity

Inferred from direct assay Ref.4PubMed 11895172PubMed 15993839. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NCK1P163332EBI-1759386,EBI-389883

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q9UHI7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UHI7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     156-156: V → VGLHV
Note: Inactive.
Isoform 3 (identifier: Q9UHI7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     92-430: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Solute carrier family 23 member 1
PRO_0000165975

Regions

Topological domain1 – 5252Cytoplasmic Potential
Transmembrane53 – 7321Helical; Potential
Topological domain74 – 818Extracellular Potential
Transmembrane82 – 10221Helical; Potential
Topological domain1031Cytoplasmic Potential
Transmembrane104 – 12421Helical; Potential
Topological domain125 – 15935Extracellular Potential
Transmembrane160 – 18021Helical; Potential
Topological domain181 – 20727Cytoplasmic Potential
Transmembrane208 – 22518Helical; Potential
Topological domain226 – 2294Extracellular Potential
Intramembrane230 – 24314Helical; Potential
Topological domain244 – 2507Extracellular Potential
Transmembrane251 – 27121Helical; Potential
Topological domain272 – 31241Cytoplasmic Potential
Transmembrane313 – 33321Helical; Potential
Topological domain334 – 35825Extracellular Potential
Transmembrane359 – 37921Helical; Potential
Topological domain380 – 40223Cytoplasmic Potential
Transmembrane403 – 42321Helical; Potential
Topological domain424 – 4263Extracellular Potential
Transmembrane427 – 44721Helical; Potential
Topological domain448 – 45710Cytoplasmic Potential
Transmembrane458 – 47821Helical; Potential
Topological domain479 – 49012Extracellular Potential
Transmembrane491 – 51121Helical; Potential
Topological domain512 – 59887Cytoplasmic Potential

Amino acid modifications

Modified residue5961Phosphothreonine By similarity
Glycosylation1381N-linked (GlcNAc...) Ref.9
Glycosylation1441N-linked (GlcNAc...) Ref.9

Natural variations

Alternative sequence92 – 430339Missing in isoform 3.
VSP_006813
Alternative sequence1561V → VGLHV in isoform 2.
VSP_006814
Natural variant2181I → V.
Corresponds to variant rs34521685 [ dbSNP | Ensembl ].
VAR_053451
Natural variant2581M → V.
Corresponds to variant rs35817838 [ dbSNP | Ensembl ].
VAR_053452
Natural variant2641V → M.
Corresponds to variant rs33972313 [ dbSNP | Ensembl ].
VAR_053453
Natural variant4211S → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.8
Corresponds to variant rs6596474 [ dbSNP | Ensembl ].
VAR_062111

Experimental info

Sequence conflict111T → A in AAF24759. Ref.2
Sequence conflict52 – 576YLTCFS → IHDCLR in AAC78804. Ref.1
Sequence conflict75 – 806DQHMVS → SQTLHC in AAC78804. Ref.1
Sequence conflict1391W → S in AAC78804. Ref.1
Sequence conflict1531I → N in AAC78804. Ref.1
Sequence conflict1551E → D in AAF22490. Ref.2
Sequence conflict182 – 1832YI → SL in AAC78804. Ref.1
Sequence conflict2071A → P in AAC78804. Ref.1
Sequence conflict2691Y → I in AAC78804. Ref.1
Sequence conflict2751D → E in AAF22490. Ref.2
Sequence conflict2841Y → I in AAC78804. Ref.1
Sequence conflict4341T → S in AAC78804. Ref.1
Sequence conflict451 – 4522DM → AL in AAC78804. Ref.1
Sequence conflict476 – 4772ES → SP in AAF22490. Ref.2
Sequence conflict5481I → F in AAC78804. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 1191B2B43CE76FD6

FASTA59864,831
        10         20         30         40         50         60 
MRAQEDLEGR TQHETTRDPS TPLPTEPKFD MLYKIEDVPP WYLCILLGFQ HYLTCFSGTI 

        70         80         90        100        110        120 
AVPFLLAEAL CVGHDQHMVS QLIGTIFTCV GITTLIQTTV GIRLPLFQAS AFAFLVPAKA 

       130        140        150        160        170        180 
ILALERWKCP PEEEIYGNWS LPLNTSHIWH PRIREVQGAI MVSSVVEVVI GLLGLPGALL 

       190        200        210        220        230        240 
NYIGPLTVTP TVSLIGLSVF QAAGDRAGSH WGISACSILL IILFSQYLRN LTFLLPVYRW 

       250        260        270        280        290        300 
GKGLTLLRIQ IFKMFPIMLA IMTVWLLCYV LTLTDVLPTD PKAYGFQART DARGDIMAIA 

       310        320        330        340        350        360 
PWIRIPYPCQ WGLPTVTAAA VLGMFSATLA GIIESIGDYY ACARLAGAPP PPVHAINRGI 

       370        380        390        400        410        420 
FTEGICCIIA GLLGTGNGST SSSPNIGVLG ITKVGSRRVV QYGAAIMLVL GTIGKFTALF 

       430        440        450        460        470        480 
SSLPDPILGG MFCTLFGMIT AVGLSNLQFV DMNSSRNLFV LGFSMFFGLT LPNYLESNPG 

       490        500        510        520        530        540 
AINTGILEVD QILIVLLTTE MFVGGCLAFI LDNTVPGSPE ERGLIQWKAG AHANSDMSSS 

       550        560        570        580        590 
LKSYDFPIGM GIVKRITFLK YIPICPVFKG FSSSSKDQIA IPEDTPENTE TASVCTKV 

« Hide

Isoform 2 [UniParc].

Checksum: 0D03E3405E2E1EB3
Show »

FASTA60265,238
Isoform 3 [UniParc].

Checksum: 1F422EEA2AE6A2C5
Show »

FASTA25928,531

References

« Hide 'large scale' references
[1]"Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters."
Faaland C.A., Race J.E., Ricken G., Warner F.J., Williams W.J., Holtzman E.J.
Biochim. Biophys. Acta 1442:353-360(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421.
Tissue: Fetal liver and Kidney.
[2]"Human Na(+)-dependent vitamin C transporter 1 (hSVCT1): primary structure, functional characteristics and evidence for a non-functional splice variant."
Wang H., Dutta B., Huang W., Devoe L.D., Leibach F.H., Ganapathy V., Prasad P.D.
Biochim. Biophys. Acta 1461:1-9(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-421.
Tissue: Intestinal epithelium.
[3]"Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2."
Daruwala R.C., Song J., Koh W.S., Rumsey S.C., Levine M.
FEBS Lett. 460:480-484(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421.
Tissue: Kidney.
[4]"Human vitamin C (L-ascorbic acid) transporter SVCT1."
Wang Y., Mackenzie B., Tsukaguchi H., Weremowicz S., Morton C.C., Hediger M.A.
Biochem. Biophys. Res. Commun. 267:488-494(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421.
Tissue: Kidney.
[5]"Characterization of the genomic structure of the human vitamin C transporter SVCT1 (SLC23A2)."
Erichsen H.C., Eck P., Levine M., Chanock S.
J. Nutr. 131:2623-2627(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), VARIANT ALA-421.
[6]"Vitamin C transport systems of mammalian cells."
Liang W.J., Johnson D., Jarvis S.M.
Mol. Membr. Biol. 18:87-95(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-421.
Tissue: Liver cancer.
[7]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT ALA-421.
Tissue: Colon and Kidney.
[9]"Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting."
Velho A.M., Jarvis S.M.
Exp. Cell Res. 315:2312-2321(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-138 AND ASN-144, SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF058317 mRNA. Translation: AAC78804.1.
AF170911 mRNA. Translation: AAF24759.1.
AJ269477 mRNA. Translation: CAB58119.1.
AF098277 mRNA. Translation: AAF22490.1.
AF375875 Genomic DNA. Translation: AAK97398.1.
AJ250807 mRNA. Translation: CAC15384.1.
AC135457 Genomic DNA. No translation available.
BC019225 mRNA. Translation: AAH19225.1.
BC050261 mRNA. Translation: AAH50261.1.
PIRJC7182.
RefSeqNP_005838.3. NM_005847.4.
NP_689898.2. NM_152685.3.
UniGeneHs.643467.

3D structure databases

ProteinModelPortalQ9UHI7.
SMRQ9UHI7. Positions 35-470.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9UHI7. 4 interactions.
STRING9606.ENSP00000302851.

Chemistry

DrugBankDB00126. Vitamin C.
GuidetoPHARMACOLOGY1041.

Protein family/group databases

TCDB2.A.40.6.5. the nucleobase:cation symporter-2 (ncs2) family.

PTM databases

PhosphoSiteQ9UHI7.

Polymorphism databases

DMDM296452969.

Proteomic databases

PaxDbQ9UHI7.
PRIDEQ9UHI7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000348729; ENSP00000302701; ENSG00000170482. [Q9UHI7-1]
ENST00000353963; ENSP00000302851; ENSG00000170482. [Q9UHI7-2]
GeneID9963.
KEGGhsa:9963.
UCSCuc003leh.3. human. [Q9UHI7-1]

Organism-specific databases

CTD9963.
GeneCardsGC05M138702.
HGNCHGNC:10974. SLC23A1.
HPAHPA047612.
MIM603790. gene.
neXtProtNX_Q9UHI7.
PharmGKBPA35850.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2233.
HOGENOMHOG000038201.
HOVERGENHBG056256.
KOK14611.
OMALDRWKCP.
OrthoDBEOG7R56RZ.
PhylomeDBQ9UHI7.
TreeFamTF313272.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.

Gene expression databases

ArrayExpressQ9UHI7.
BgeeQ9UHI7.
CleanExHS_SLC23A1.
GenevestigatorQ9UHI7.

Family and domain databases

InterProIPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERPTHR11119. PTHR11119. 1 hit.
PfamPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSLC23A1.
GenomeRNAi9963.
NextBio37598.
PROQ9UHI7.
SOURCESearch...

Entry information

Entry nameS23A1_HUMAN
AccessionPrimary (citable) accession number: Q9UHI7
Secondary accession number(s): O95191 expand/collapse secondary AC list , Q8WWB6, Q9UGH4, Q9UI39
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM