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Protein

Large neutral amino acids transporter small subunit 2

Gene

SLC7A8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.8 Publications

Kineticsi

  1. KM=221 µM for L-leucine4 Publications
  2. KM=64 µM for MeHg-L-cysteine4 Publications
  3. KM=161 µM for methionine4 Publications
  4. KM=978 µM for L-alanine4 Publications
  5. KM=89.35 µM for L-phenylalanine4 Publications
  6. KM=57.3 µM for L-tryptophan4 Publications
  7. KM=48.8 µM for L-tyrosine4 Publications

    GO - Molecular functioni

    • amino acid transmembrane transporter activity Source: UniProtKB
    • antiporter activity Source: GO_Central
    • L-amino acid transmembrane transporter activity Source: GO_Central
    • neutral amino acid transmembrane transporter activity Source: ProtInc
    • organic cation transmembrane transporter activity Source: UniProtKB
    • peptide antigen binding Source: UniProtKB
    • toxin transporter activity Source: UniProtKB

    GO - Biological processi

    • amino acid transmembrane transport Source: GO_Central
    • amino acid transport Source: UniProtKB
    • blood coagulation Source: Reactome
    • cellular amino acid metabolic process Source: ProtInc
    • ion transport Source: Reactome
    • leukocyte migration Source: Reactome
    • metal ion homeostasis Source: UniProtKB
    • neutral amino acid transport Source: UniProtKB
    • organic cation transport Source: GOC
    • response to toxic substance Source: UniProtKB
    • toxin transport Source: GOC
    • transmembrane transport Source: Reactome
    • transport Source: ProtInc
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000092068-MONOMER.
    ReactomeiREACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RKQ9UHI5.

    Protein family/group databases

    TCDBi2.A.3.8.20. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 2
    Alternative name(s):
    L-type amino acid transporter 2
    Short name:
    hLAT2
    Solute carrier family 7 member 8
    Gene namesi
    Name:SLC7A8
    Synonyms:LAT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:11066. SLC7A8.

    Subcellular locationi

    • Cytoplasm
    • Basolateral cell membrane; Multi-pass membrane protein

    • Note: Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei72 – 9221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei155 – 17521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei231 – 25121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei268 – 28821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 33021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei388 – 40821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei424 – 44421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei447 – 46721HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • basolateral plasma membrane Source: UniProtKB-SubCell
    • cytoplasm Source: UniProtKB-SubCell
    • extracellular exosome Source: UniProtKB
    • integral component of plasma membrane Source: ProtInc
    • plasma membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35926.

    Chemistry

    DrugBankiDB00160. L-Alanine.
    DB01235. L-DOPA.
    DB00130. L-Glutamine.
    DB00120. L-Phenylalanine.

    Polymorphism and mutation databases

    BioMutaiSLC7A8.
    DMDMi12643348.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Large neutral amino acids transporter small subunit 2PRO_0000054273Add
    BLAST

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ9UHI5.
    PRIDEiQ9UHI5.

    PTM databases

    PhosphoSiteiQ9UHI5.

    Expressioni

    Tissue specificityi

    Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2.4 Publications

    Inductioni

    Activity in polarized intestinal cells is regulated by the association between SLC3A2/4F2 (in the SLC3A2/4F2-LAT2 heterodimer) and ICAM1.1 Publication

    Gene expression databases

    BgeeiQ9UHI5.
    CleanExiHS_LAT2.
    HS_SLC7A8.
    ExpressionAtlasiQ9UHI5. baseline and differential.
    GenevisibleiQ9UHI5. HS.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.6 Publications

    Protein-protein interaction databases

    BioGridi116996. 2 interactions.
    STRINGi9606.ENSP00000320378.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UHI5.
    SMRiQ9UHI5. Positions 39-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9UHI5.
    KOiK13781.
    OMAiEVYGEFW.
    PhylomeDBiQ9UHI5.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9UHI5-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MEEGARHRNN TEKKHPGGGE SDASPEAGSG GGGVALKKEI GLVSACGIIV
    60 70 80 90 100
    GNIIGSGIFV SPKGVLENAG SVGLALIVWI VTGFITVVGA LCYAELGVTI
    110 120 130 140 150
    PKSGGDYSYV KDIFGGLAGF LRLWIAVLVI YPTNQAVIAL TFSNYVLQPL
    160 170 180 190 200
    FPTCFPPESG LRLLAAICLL LLTWVNCSSV RWATRVQDIF TAGKLLALAL
    210 220 230 240 250
    IIIMGIVQIC KGEYFWLEPK NAFENFQEPD IGLVALAFLQ GSFAYGGWNF
    260 270 280 290 300
    LNYVTEELVD PYKNLPRAIF ISIPLVTFVY VFANVAYVTA MSPQELLASN
    310 320 330 340 350
    AVAVTFGEKL LGVMAWIMPI SVALSTFGGV NGSLFTSSRL FFAGAREGHL
    360 370 380 390 400
    PSVLAMIHVK RCTPIPALLF TCISTLLMLV TSDMYTLINY VGFINYLFYG
    410 420 430 440 450
    VTVAGQIVLR WKKPDIPRPI KINLLFPIIY LLFWAFLLVF SLWSEPVVCG
    460 470 480 490 500
    IGLAIMLTGV PVYFLGVYWQ HKPKCFSDFI ELLTLVSQKM CVVVYPEVER
    510 520 530
    GSGTEEANED MEEQQQPMYQ PTPTKDKDVA GQPQP
    Length:535
    Mass (Da):58,382
    Last modified:May 1, 2000 - v1
    Checksum:iAC129146353F1E47
    GO
    Isoform 2 (identifier: Q9UHI5-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-203: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:332
    Mass (Da):37,184
    Checksum:iE6DD8F71252E064D
    GO
    Isoform 3 (identifier: Q9UHI5-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-263: MEEGARHRNN...VTEELVDPYK → MGQLFQCAVG...HHGDCTDMQR

    Note: No experimental confirmation available.
    Show »
    Length:311
    Mass (Da):34,602
    Checksum:i4CF5A20AD230155C
    GO
    Isoform 4 (identifier: Q9UHI5-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-169: MEEGARHRNN...GLRLLAAICL → MGQYGQELSW...LEGVPRFLKR

    Note: No experimental confirmation available.
    Show »
    Length:430
    Mass (Da):48,283
    Checksum:i4F7293A07EBFE696
    GO

    Sequence cautioni

    The sequence CAD62616.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti225 – 2251N → D in CAB40137 (PubMed:10080183).Curated
    Sequence conflicti401 – 4011V → G in CAB40137 (PubMed:10080183).Curated
    Sequence conflicti503 – 5031G → R in AAF05696 (PubMed:10391915).Curated
    Sequence conflicti503 – 5031G → R in AAF05697 (PubMed:10391915).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 263263MEEGA…VDPYK → MGQLFQCAVGHPGSRHLHSW EAPGLGPDYHHGDCTDMQR in isoform 3. 1 PublicationVSP_046945Add
    BLAST
    Alternative sequencei1 – 203203Missing in isoform 2. 1 PublicationVSP_046946Add
    BLAST
    Alternative sequencei1 – 169169MEEGA…AAICL → MGQYGQELSWKCLVKAVCLQ EHSQPSQLLCTLLLCWCVLG RERPFRKAQSTSSPLEGVPR FLKR in isoform 4. 1 PublicationVSP_046947Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF135828 mRNA. Translation: AAF05695.1.
    AF135829 mRNA. Translation: AAF05696.1.
    AF135830 mRNA. Translation: AAF05697.1.
    AF171669 mRNA. Translation: AAF20381.1.
    Y18483 mRNA. Translation: CAB40137.1.
    AB037669 mRNA. Translation: BAB21519.1.
    BX248288 mRNA. Translation: CAD62616.1. Different initiation.
    AK296702 mRNA. Translation: BAG59296.1.
    AK300384 mRNA. Translation: BAG62118.1.
    AK313465 mRNA. Translation: BAG36251.1.
    AL117258 Genomic DNA. No translation available.
    CH471078 Genomic DNA. Translation: EAW66181.1.
    CH471078 Genomic DNA. Translation: EAW66182.1.
    BC052250 mRNA. Translation: AAH52250.1.
    CCDSiCCDS41924.1. [Q9UHI5-2]
    CCDS58304.1. [Q9UHI5-3]
    CCDS58305.1. [Q9UHI5-4]
    CCDS9590.1. [Q9UHI5-1]
    RefSeqiNP_001253965.1. NM_001267036.1. [Q9UHI5-4]
    NP_001253966.1. NM_001267037.1. [Q9UHI5-3]
    NP_036376.2. NM_012244.3. [Q9UHI5-1]
    NP_877392.1. NM_182728.2. [Q9UHI5-2]
    UniGeneiHs.596643.

    Genome annotation databases

    EnsembliENST00000316902; ENSP00000320378; ENSG00000092068. [Q9UHI5-1]
    ENST00000422941; ENSP00000416398; ENSG00000092068. [Q9UHI5-3]
    ENST00000453702; ENSP00000391577; ENSG00000092068. [Q9UHI5-2]
    ENST00000529705; ENSP00000434345; ENSG00000092068. [Q9UHI5-4]
    GeneIDi23428.
    KEGGihsa:23428.
    UCSCiuc001wix.4. human. [Q9UHI5-1]
    uc010tnk.3. human.
    uc010tnl.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF135828 mRNA. Translation: AAF05695.1.
    AF135829 mRNA. Translation: AAF05696.1.
    AF135830 mRNA. Translation: AAF05697.1.
    AF171669 mRNA. Translation: AAF20381.1.
    Y18483 mRNA. Translation: CAB40137.1.
    AB037669 mRNA. Translation: BAB21519.1.
    BX248288 mRNA. Translation: CAD62616.1. Different initiation.
    AK296702 mRNA. Translation: BAG59296.1.
    AK300384 mRNA. Translation: BAG62118.1.
    AK313465 mRNA. Translation: BAG36251.1.
    AL117258 Genomic DNA. No translation available.
    CH471078 Genomic DNA. Translation: EAW66181.1.
    CH471078 Genomic DNA. Translation: EAW66182.1.
    BC052250 mRNA. Translation: AAH52250.1.
    CCDSiCCDS41924.1. [Q9UHI5-2]
    CCDS58304.1. [Q9UHI5-3]
    CCDS58305.1. [Q9UHI5-4]
    CCDS9590.1. [Q9UHI5-1]
    RefSeqiNP_001253965.1. NM_001267036.1. [Q9UHI5-4]
    NP_001253966.1. NM_001267037.1. [Q9UHI5-3]
    NP_036376.2. NM_012244.3. [Q9UHI5-1]
    NP_877392.1. NM_182728.2. [Q9UHI5-2]
    UniGeneiHs.596643.

    3D structure databases

    ProteinModelPortaliQ9UHI5.
    SMRiQ9UHI5. Positions 39-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi116996. 2 interactions.
    STRINGi9606.ENSP00000320378.

    Chemistry

    DrugBankiDB00160. L-Alanine.
    DB01235. L-DOPA.
    DB00130. L-Glutamine.
    DB00120. L-Phenylalanine.

    Protein family/group databases

    TCDBi2.A.3.8.20. the amino acid-polyamine-organocation (apc) family.

    PTM databases

    PhosphoSiteiQ9UHI5.

    Polymorphism and mutation databases

    BioMutaiSLC7A8.
    DMDMi12643348.

    Proteomic databases

    PaxDbiQ9UHI5.
    PRIDEiQ9UHI5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000316902; ENSP00000320378; ENSG00000092068. [Q9UHI5-1]
    ENST00000422941; ENSP00000416398; ENSG00000092068. [Q9UHI5-3]
    ENST00000453702; ENSP00000391577; ENSG00000092068. [Q9UHI5-2]
    ENST00000529705; ENSP00000434345; ENSG00000092068. [Q9UHI5-4]
    GeneIDi23428.
    KEGGihsa:23428.
    UCSCiuc001wix.4. human. [Q9UHI5-1]
    uc010tnk.3. human.
    uc010tnl.3. human.

    Organism-specific databases

    CTDi23428.
    GeneCardsiGC14M023594.
    HGNCiHGNC:11066. SLC7A8.
    MIMi604235. gene.
    neXtProtiNX_Q9UHI5.
    PharmGKBiPA35926.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9UHI5.
    KOiK13781.
    OMAiEVYGEFW.
    PhylomeDBiQ9UHI5.
    TreeFamiTF313355.

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000092068-MONOMER.
    ReactomeiREACT_12560. Basigin interactions.
    REACT_13796. Amino acid transport across the plasma membrane.
    SABIO-RKQ9UHI5.

    Miscellaneous databases

    ChiTaRSiSLC7A8. human.
    GeneWikiiSLC7A8.
    GenomeRNAii23428.
    NextBioi45663.
    PROiQ9UHI5.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9UHI5.
    CleanExiHS_LAT2.
    HS_SLC7A8.
    ExpressionAtlasiQ9UHI5. baseline and differential.
    GenevisibleiQ9UHI5. HS.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids."
      Pineda M., Fernandez E., Torrents D., Estevez R., Lopez C., Camps M., Lloberas J., Zorzano A., Palacin M.
      J. Biol. Chem. 274:19738-19744(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.
    2. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
      Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
      J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT.
      Tissue: Melanocyte.
    3. "SLC7A7, encoding a putative permease-related protein, is mutated in patients with lysinuric protein intolerance."
      Borsani G., Bassi M.T., Sperandeo M.P., De Grandi A., Buoninconti A., Riboni M., Manzoni M., Incerti B., Pepe A., Andria G., Ballabio A., Sebastio G.
      Nat. Genet. 21:297-301(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Placenta.
    4. "Reabsorption of neutral amino acids mediated by amino acid transporter LAT2 and TAT1 in the basolateral membrane of proximal tubule."
      Park S.Y., Kim J.-K., Kim I.J., Choi B.K., Jung K.Y., Lee S., Park K.J., Chairoungdua A., Kanai Y., Endou H., Kim D.K.
      Arch. Pharm. Res. 28:421-432(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INHIBITION.
      Tissue: Kidney.
    5. "Full-length cDNA libraries and normalization."
      Li W.B., Gruber C., Jessee J., Polayes D.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Placenta.
    6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
      Tissue: Hippocampus, Placenta and Tongue.
    7. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    10. "Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
      Broeer A., Friedrich B., Wagner C.A., Fillon S., Ganapathy V., Lang F., Broeer S.
      Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    11. "Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
      Simmons-Willis T.A., Koh A.S., Clarkson T.W., Ballatori N.
      Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION.
    12. "CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia."
      Liu X., Charrier L., Gewirtz A., Sitaraman S., Merlin D.
      J. Biol. Chem. 278:23672-23677(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INDUCTION.
    13. "Identification and functional characterization of a Na(+)-independent large neutral amino acid transporter (LAT2) on ARPE-19 cells."
      Gandhi M.D., Pal D., Mitra A.K.
      Int. J. Pharm. 275:189-200(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INHIBITION.
    14. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
      Fraga S., Pinho M.J., Soares-da-Silva P.
      Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    15. "Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
      Li S., Whorton A.R.
      J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INHIBITION.

    Entry informationi

    Entry nameiLAT2_HUMAN
    AccessioniPrimary (citable) accession number: Q9UHI5
    Secondary accession number(s): B2R8Q4
    , B4DKT4, B4DTV6, D3DS46, F2Z2J4, Q86U05, Q9UKQ6, Q9UKQ7, Q9UKQ8, Q9Y445
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: May 1, 2000
    Last modified: June 24, 2015
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    L-leucine transport activity inhibited by small zwitterionic amino acids (i.e. glycine, alanine, serine, threonine asparginine, glutamine, methionine, leucine, isoleucine, valine, phenylalanine, tyrosine, tryptophan, histidine and cysteine) and by glutamine and asparginine. Methionine uptake was inhibited by the L-system substrates L-leucine, 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), L-cysteine and by the MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.