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Q9UHG3 (PCYOX_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prenylcysteine oxidase 1

EC=1.8.3.5
Alternative name(s):
Prenylcysteine lyase
Gene names
Name:PCYOX1
Synonyms:KIAA0908, PCL1
ORF Names:UNQ597/PRO1183
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.

Catalytic activity

An S-prenyl-L-cysteine + O2 + H2O = a prenal + L-cysteine + H2O2.

Cofactor

FAD.

Subcellular location

Lysosome.

Tissue specificity

Ubiquitous.

Post-translational modification

The protein is glycosylated at one or more potential N-glycosylation sites.

Sequence similarities

Belongs to the prenylcysteine oxidase family.

Caution

Was originally (Ref.1) thought to be a lyase and was therefore termed prenylcysteine lyase.

Sequence caution

The sequence BAA74931.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 505478Prenylcysteine oxidase 1
PRO_0000023298

Amino acid modifications

Glycosylation1961N-linked (GlcNAc...) Ref.8 Ref.10
Glycosylation3231N-linked (GlcNAc...) Ref.8
Glycosylation3531N-linked (GlcNAc...) Ref.8 Ref.10
Cross-link162Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9

Natural variations

Natural variant1491S → F. Ref.2
Corresponds to variant rs2706762 [ dbSNP | Ensembl ].
VAR_050469
Natural variant4141T → S.
Corresponds to variant rs17005441 [ dbSNP | Ensembl ].
VAR_050470
Natural variant4651S → G.
Corresponds to variant rs34041544 [ dbSNP | Ensembl ].
VAR_050471

Experimental info

Sequence conflict3411Q → H in AAF16937. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9UHG3 [UniParc].

Last modified September 19, 2006. Version 3.
Checksum: FB388AF70AF229F5

FASTA50556,640
        10         20         30         40         50         60 
MGRVVAELVS SLLGLWLLLC SCGCPEGAEL RAPPDKIAII GAGIGGTSAA YYLRQKFGKD 

        70         80         90        100        110        120 
VKIDLFEREE VGGRLATMMV QGQEYEAGGS VIHPLNLHMK RFVKDLGLSA VQASGGLLGI 

       130        140        150        160        170        180 
YNGETLVFEE SNWFIINVIK LVWRYGFQSL RMHMWVEDVL DKFMRIYRYQ SHDYAFSSVE 

       190        200        210        220        230        240 
KLLHALGGDD FLGMLNRTLL ETLQKAGFSE KFLNEMIAPV MRVNYGQSTD INAFVGAVSL 

       250        260        270        280        290        300 
SCSDSGLWAV EGGNKLVCSG LLQASKSNLI SGSVMYIEEK TKTKYTGNPT KMYEVVYQIG 

       310        320        330        340        350        360 
TETRSDFYDI VLVATPLNRK MSNITFLNFD PPIEEFHQYY QHIVTTLVKG ELNTSIFSSR 

       370        380        390        400        410        420 
PIDKFGLNTV LTTDNSDLFI NSIGIVPSVR EKEDPEPSTD GTYVWKIFSQ ETLTKAQILK 

       430        440        450        460        470        480 
LFLSYDYAVK KPWLAYPHYK PPEKCPSIIL HDRLYYLNGI ECAASAMEMS AIAAHNAALL 

       490        500 
AYHRWNGHTD MIDQDGLYEK LKTEL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and cellular localization of a human prenylcysteine lyase."
Tschantz W.R., Zhang L., Casey P.J.
J. Biol. Chem. 274:35802-35808(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PHE-149.
Tissue: Brain.
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart and Lung.
[7]Bienvenut W.V.
Submitted (JUN-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 37-54; 256-266 AND 421-430, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[8]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-196; ASN-323 AND ASN-353.
Tissue: Plasma.
[9]"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-162.
Tissue: Mammary cancer.
[10]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-196 AND ASN-353.
Tissue: Liver.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF181490 mRNA. Translation: AAF16937.1.
AB020715 mRNA. Translation: BAA74931.1. Different initiation.
AY359063 mRNA. Translation: AAQ89422.1.
AK314453 mRNA. Translation: BAG37061.1.
CH471053 Genomic DNA. Translation: EAW99819.1.
BC007029 mRNA. Translation: AAH07029.1.
BC033815 mRNA. Translation: AAH33815.1.
BC051891 mRNA. Translation: AAH51891.1.
CCDSCCDS1902.1.
RefSeqNP_057381.3. NM_016297.3.
UniGeneHs.567502.

3D structure databases

ProteinModelPortalQ9UHG3.
SMRQ9UHG3. Positions 35-110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119547. 4 interactions.
IntActQ9UHG3. 2 interactions.
STRING9606.ENSP00000387654.

PTM databases

PhosphoSiteQ9UHG3.

Polymorphism databases

DMDM115311617.

Proteomic databases

MaxQBQ9UHG3.
PaxDbQ9UHG3.
PeptideAtlasQ9UHG3.
PRIDEQ9UHG3.

Protocols and materials databases

DNASU51449.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000433351; ENSP00000387654; ENSG00000116005.
GeneID51449.
KEGGhsa:51449.
UCSCuc002sgn.4. human.

Organism-specific databases

CTD51449.
GeneCardsGC02P070484.
HGNCHGNC:20588. PCYOX1.
HPAHPA035193.
MIM610995. gene.
neXtProtNX_Q9UHG3.
PharmGKBPA134959852.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG73316.
HOGENOMHOG000241149.
HOVERGENHBG053532.
InParanoidQ9UHG3.
KOK05906.
OMANGIECAA.
PhylomeDBQ9UHG3.
TreeFamTF329001.

Gene expression databases

ArrayExpressQ9UHG3.
BgeeQ9UHG3.
CleanExHS_PCYOX1.
GenevestigatorQ9UHG3.

Family and domain databases

InterProIPR010795. Prenylcys_lyase.
IPR017046. Prenylcysteine_Oxase.
[Graphical view]
PfamPF07156. Prenylcys_lyase. 1 hit.
[Graphical view]
PIRSFPIRSF036292. Prenylcysteine_oxidase. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPCYOX1. human.
GeneWikiPCYOX1.
GenomeRNAi51449.
NextBio55051.
PROQ9UHG3.
SOURCESearch...

Entry information

Entry namePCYOX_HUMAN
AccessionPrimary (citable) accession number: Q9UHG3
Secondary accession number(s): B2RB14 expand/collapse secondary AC list , O94982, Q8N4N5, Q96QM8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 19, 2006
Last modified: July 9, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM