Q9UHF0 (TKNK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tachykinin-3

Alternative name(s):
ZNEUROK1

Cleaved into the following chain:

1. Neurokinin-B
   Short name=NKB
   Alternative name(s):
   Neuromedin-K

Gene names

Name:	TAC3
Synonyms:	NKNB
ORF Names:	UNQ585/PRO1155

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles By similarity.
Subcellular location	Secreted.
Developmental stage	In pregnancy, the expression of NKB is confined to the outer syncytiotrophoblast of the placenta, significant concentrations of NKB can be detected in plasma as early as week 9, and plasma concentrations of NKB are grossly elevated in pregnancy-induced hypertension and pre-eclampsia.
Sequence similarities	Belongs to the tachykinin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	female pregnancy Traceable author statement. Source: ProtInc neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW tachykinin receptor signaling pathway Traceable author statement. Source: ProtInc
Cellular component	extracellular space Traceable author statement. Source: ProtInc soluble fraction Traceable author statement. Source: ProtInc
Molecular function	receptor binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UHF0-1)

Also known as: Beta tachykinin 3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHF0-2)

Also known as: Alpha tachykinin 3;

The sequence of this isoform differs from the canonical sequence as follows:
     98-121: DSPTDVNQENVPSFGILKYPPRAE → EGKTGPFLPSVRVPRPLHPNQLGSTGKSSLGTEEQRPL

Isoform 3 (identifier: Q9UHF0-3)

Also known as: Gamma tachykinin 3;

The sequence of this isoform differs from the canonical sequence as follows:
     80-98: RDMHDFFVGLMGKRSVQPD → H

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Potential

Propeptide

17 – 78

62

By similarity

PRO_0000033565

Peptide

81 – 90

10

Neurokinin-B

PRO_0000033566

Propeptide

94 – 121

28

By similarity

PRO_0000033567

Amino acid modifications

Modified residue

90

1

Methionine amide By similarity

Natural variations

Alternative sequence

80 – 98

19

RDMHD…SVQPD → H in isoform 3.

VSP_013186

Alternative sequence

98 – 121

24

DSPTD…PPRAE → EGKTGPFLPSVRVPRPLHPN QLGSTGKSSLGTEEQRPL in isoform 2.

VSP_013187

Experimental info

Sequence conflict

121

1

E → D in CAG33474. Ref.5

Secondary structure

1

.

.

.

121

Helix Strand Turn

Details...

Helix

82 – 89

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Beta tachykinin 3) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 14C9AFE2EE9EDECA	FASTA	121	13,438
	10 20 30 40 50 60 MRIMLLFTAI LAFSLAQSFG AVCKEPQEEV VPGGGRSKRD PDLYQLLQRL FKSHSSLEGL 70 80 90 100 110 120 LKALSQASTD PKESTSPEKR DMHDFFVGLM GKRSVQPDSP TDVNQENVPS FGILKYPPRA E
	Isoform 2 (Alpha tachykinin 3) [UniParc]. Checksum: 4C6DBBDBE30851F3	FASTA	135	14,833
	10 20 30 40 50 60 MRIMLLFTAI LAFSLAQSFG AVCKEPQEEV VPGGGRSKRD PDLYQLLQRL FKSHSSLEGL 70 80 90 100 110 120 LKALSQASTD PKESTSPEKR DMHDFFVGLM GKRSVQPEGK TGPFLPSVRV PRPLHPNQLG 130 STGKSSLGTE EQRPL
	Isoform 3 (Gamma tachykinin 3) [UniParc]. Checksum: 2D0026B586B03FC6	FASTA	103	11,358
	10 20 30 40 50 60 MRIMLLFTAI LAFSLAQSFG AVCKEPQEEV VPGGGRSKRD PDLYQLLQRL FKSHSSLEGL 70 80 90 100 LKALSQASTD PKESTSPEKH SPTDVNQENV PSFGILKYPP RAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Excessive placental secretion of neurokinin B during the third trimester causes pre-eclampsia." Page N.M., Woods R.J., Gardiner S.M., Lomthiasong K., Gladwell R.T., Butlin D.J., Manyonda I.T., Lowry P.J. Nature 405:797-800(2000) [PubMed: 10866201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[3]	"The peripheral expression of neurokinin B, its splice variants and its receptors." Bell N.J., Page N.M. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF186112 mRNA. Translation: AAF01430.1.
AF216586 mRNA. Translation: AAF76980.1.
AF537113 mRNA. Translation: AAQ10783.1.
AF537114 mRNA. Translation: AAQ10784.1.
AF537115 mRNA. Translation: AAQ10785.1.
AF537116 mRNA. Translation: AAQ10786.1.
AF537117 mRNA. Translation: AAQ10787.1.
AF537118 mRNA. Translation: AAQ10788.1.
AF537119 mRNA. Translation: AAQ10789.1.
AF537120 mRNA. Translation: AAQ10790.1.
AF537121 mRNA. Translation: AAQ10791.1.
AY358679 mRNA. Translation: AAQ89042.1.
CR457193 mRNA. Translation: CAG33474.1.
BC032145 mRNA. Translation: AAH32145.1.

IPI

IPI00001674.
IPI00431183.
IPI00554743.

RefSeq

NP_001171525.1. NM_001178054.1.
NP_037383.1. NM_013251.3.

UniGene

Hs.9730.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1P9F	NMR	-	A	81-90	[»]

ProteinModelPortal

Q9UHF0.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UHF0. 6 interactions.

MINT

MINT-1381829.

STRING

Q9UHF0.

Polymorphism databases

DMDM

18203501.

Proteomic databases

PRIDE

Q9UHF0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000300108; ENSP00000300108; ENSG00000166863.
ENST00000415231; ENSP00000402995; ENSG00000166863.
ENST00000458521; ENSP00000404056; ENSG00000166863.

GeneID

6866.

KEGG

hsa:6866.

UCSC

uc001smm.1. human.
uc001smn.1. human.
uc001smo.1. human.

Organism-specific databases

CTD

6866.

GeneCards

GC12M057403.

H-InvDB

HIX0010742.

HGNC

HGNC:11521. TAC3.

HPA

HPA045919.

MIM

162330. gene.

neXtProt

NX_Q9UHF0.

Orphanet

432. Normosmic congenital hypogonadotropic hypogonadism.

PharmGKB

PA36298.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000000335.

HOVERGEN

HBG016587.

OMA

TDVNQEN.

OrthoDB

EOG4HDSW3.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

Q9UHF0.

Bgee

Q9UHF0.

CleanEx

HS_TAC3.

Genevestigator

Q9UHF0.

GermOnline

ENSG00000166863. Homo sapiens.

Family and domain databases

InterPro

IPR003635. Neurokinin.
IPR013055. Tachy_Neuro_lke_CS.
[Graphical view]

KO

K05240.

PANTHER

PTHR15536. Neurokinin. 1 hit.

Pfam

PF03823. Neurokinin_B. 1 hit.
[Graphical view]

PIRSF

PIRSF001843. Neurokinin. 1 hit.

PRINTS

PR01828. NEUROKININB.

PROSITE

PS00267. TACHYKININ. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

26797.

PMAP-CutDB

Q9UHF0.

SOURCE

Search...

Entry information

Entry name

TKNK_HUMAN

Accession

Primary (citable) accession number: Q9UHF0
Secondary accession number(s): Q6IAG2, Q71BC6, Q71BC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.