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Q9UHE5

- NAT8_HUMAN

UniProt

Q9UHE5 - NAT8_HUMAN

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Protein

N-acetyltransferase 8

Gene

NAT8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed:20392701). This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617). May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).3 Publications

Catalytic activityi

Acetyl-CoA + an L-cysteine-S-conjugate = CoA + an N-acetyl L-cysteine-S-conjugate.1 Publication

Kineticsi

A Vmax of 4.4 nmol/min/mg enzyme toward S-benzyl-L-cysteine was estimated using crude cell extracts. A Vmax of 3.1 nmol/min/mg enzyme toward acetyl-CoA was estimated using crude cell extracts.

  1. KM=64 µM for S-benzyl-L-cysteine (at 37 degrees Celsius)1 Publication
  2. KM=23 µM for acetyl-CoA (at 37 degrees Celsius)1 Publication

Pathwayi

GO - Molecular functioni

  1. cysteine-S-conjugate N-acetyltransferase activity Source: UniProtKB
  2. lysine N-acetyltransferase activity, acting on acetyl phosphate as donor Source: UniProtKB

GO - Biological processi

  1. beta-amyloid metabolic process Source: UniProtKB
  2. glutathione metabolic process Source: UniProtKB
  3. negative regulation of apoptotic process Source: UniProtKB
  4. peptidyl-lysine N6-acetylation Source: UniProtKB
  5. positive regulation of gene expression Source: UniProtKB
  6. response to drug Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00204.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyltransferase 8 (EC:2.3.1.-)
Alternative name(s):
Acetyltransferase 2
Short name:
ATase2
Camello-like protein 1
Cysteinyl-conjugate N-acetyltransferase (EC:2.3.1.80)
Short name:
CCNAT
Gene namesi
Name:NAT8Imported
Synonyms:CML1Imported, GLAImported, TSC501Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:18069. NAT8.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  2. endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB
  3. endoplasmic reticulum membrane Source: UniProtKB
  4. extracellular vesicular exosome Source: UniProt
  5. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi149 – 1491R → K: Loss of the cysteine S-conjugate N-acetyltransferase activity. No effect on protein expression. 1 Publication

Organism-specific databases

PharmGKBiPA31450.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227N-acetyltransferase 8PRO_0000284684Add
BLAST

Proteomic databases

MaxQBiQ9UHE5.
PaxDbiQ9UHE5.
PRIDEiQ9UHE5.

Expressioni

Tissue specificityi

Preferentially expressed in liver and kidney. Also detected in brain (at protein level).2 Publications

Inductioni

Up-regulated by ceramides.1 Publication

Gene expression databases

BgeeiQ9UHE5.
CleanExiHS_GLA.
HS_NAT8.
GenevestigatoriQ9UHE5.

Organism-specific databases

HPAiCAB045990.

Interactioni

Subunit structurei

Interacts with PROM1. Interacts with BACE1.2 Publications

Protein-protein interaction databases

IntActiQ9UHE5. 1 interaction.
STRINGi9606.ENSP00000272425.

Structurei

3D structure databases

ProteinModelPortaliQ9UHE5.
SMRiQ9UHE5. Positions 86-195.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini64 – 227164LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei43 – 6321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 220160N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the camello family.1 Publication
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG236460.
GeneTreeiENSGT00390000009559.
HOGENOMiHOG000261685.
HOVERGENiHBG060476.
InParanoidiQ9UHE5.
OMAiWFLAKKP.
OrthoDBiEOG73Z2W2.
PhylomeDBiQ9UHE5.
TreeFamiTF324687.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UHE5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPCHIRKYQ ESDRQWVVGL LSRGMAEHAP ATFRQLLKLP RTLILLLGGP
60 70 80 90 100
LALLLVSGSW LLALVFSISL FPALWFLAKK PWTEYVDMTL CTDMSDITKS
110 120 130 140 150
YLSERGSCFW VAESEEKVVG MVGALPVDDP TLREKRLQLF HLFVDSEHRR
160 170 180 190 200
QGIAKALVRT VLQFARDQGY SEVILDTGTI QLSAMALYQS MGFKKTGQSF
210 220
FCVWARLVAL HTVHFIYHLP SSKVGSL
Length:227
Mass (Da):25,619
Last modified:April 17, 2007 - v2
Checksum:iB6CEAC98EFB0D6C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021C → R in CAG28538. 1 PublicationCurated
Sequence conflicti212 – 2121T → K in BAA34386. 1 PublicationCurated
Sequence conflicti227 – 2271L → Q in AAH12626. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti104 – 1041E → K No effect on the cysteine S-conjugate N-acetyltransferase activity.
Corresponds to variant rs13424561 [ dbSNP | Ensembl ].
VAR_053886
Natural varianti143 – 1431F → S No effect on the cysteine S-conjugate N-acetyltransferase activity. 3 Publications
Corresponds to variant rs13538 [ dbSNP | Ensembl ].
VAR_031805

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013094 mRNA. Translation: BAA33679.1.
AF187813 mRNA. Translation: AAF22303.1.
AB019551 mRNA. Translation: BAA34386.1.
CR407610 mRNA. Translation: CAG28538.1.
BC012626 mRNA. Translation: AAH12626.1.
CCDSiCCDS1926.1.
PIRiT44342.
RefSeqiNP_003951.3. NM_003960.3.
UniGeneiHs.14637.

Genome annotation databases

EnsembliENST00000272425; ENSP00000272425; ENSG00000144035.
GeneIDi9027.
KEGGihsa:9027.
UCSCiuc002sji.1. human.

Polymorphism databases

DMDMi145566894.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB013094 mRNA. Translation: BAA33679.1 .
AF187813 mRNA. Translation: AAF22303.1 .
AB019551 mRNA. Translation: BAA34386.1 .
CR407610 mRNA. Translation: CAG28538.1 .
BC012626 mRNA. Translation: AAH12626.1 .
CCDSi CCDS1926.1.
PIRi T44342.
RefSeqi NP_003951.3. NM_003960.3.
UniGenei Hs.14637.

3D structure databases

ProteinModelPortali Q9UHE5.
SMRi Q9UHE5. Positions 86-195.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9UHE5. 1 interaction.
STRINGi 9606.ENSP00000272425.

Polymorphism databases

DMDMi 145566894.

Proteomic databases

MaxQBi Q9UHE5.
PaxDbi Q9UHE5.
PRIDEi Q9UHE5.

Protocols and materials databases

DNASUi 9027.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000272425 ; ENSP00000272425 ; ENSG00000144035 .
GeneIDi 9027.
KEGGi hsa:9027.
UCSCi uc002sji.1. human.

Organism-specific databases

CTDi 9027.
GeneCardsi GC02M073779.
HGNCi HGNC:18069. NAT8.
HPAi CAB045990.
MIMi 606716. gene.
neXtProti NX_Q9UHE5.
PharmGKBi PA31450.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG236460.
GeneTreei ENSGT00390000009559.
HOGENOMi HOG000261685.
HOVERGENi HBG060476.
InParanoidi Q9UHE5.
OMAi WFLAKKP.
OrthoDBi EOG73Z2W2.
PhylomeDBi Q9UHE5.
TreeFami TF324687.

Enzyme and pathway databases

UniPathwayi UPA00204 .

Miscellaneous databases

GeneWikii NAT8.
GenomeRNAii 9027.
NextBioi 33825.
PROi Q9UHE5.
SOURCEi Search...

Gene expression databases

Bgeei Q9UHE5.
CleanExi HS_GLA.
HS_NAT8.
Genevestigatori Q9UHE5.

Family and domain databases

Gene3Di 3.40.630.30. 2 hits.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view ]
Pfami PF00583. Acetyltransf_1. 1 hit.
[Graphical view ]
SUPFAMi SSF55729. SSF55729. 1 hit.
PROSITEi PS51186. GNAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and mapping of a novel human kidney- and liver-specific gene homologous to the bacterial acetyltransferases."
    Ozaki K., Fujiwara T., Nakamura Y., Takahashi E.
    J. Hum. Genet. 43:255-258(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Kidney1 Publication.
  2. "Overexpression of camello, a member of a novel protein family, reduces blastomere adhesion and inhibits gastrulation in Xenopus laevis."
    Popsueva A.E., Luchinskaya N.N., Ludwig A.V., Zinovjeva O.Y., Poteryaev D.A., Feigelman M.M., Ponomarev M.B., Berekelya L., Belyavsky A.V.
    Dev. Biol. 234:483-496(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-143.
  3. "GLA, a kidney specific membrane protein highly expressed in renal tubular cells; possible involvement in the chronic renal failure."
    Yamamoto H., Takahashi M., Yoshimoto M., Hara H., Kitamura K., Nakagawa J.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-143.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-143.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: KidneyImported.
  6. "Two endoplasmic reticulum (ER)/ER Golgi intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels."
    Ko M.H., Puglielli L.
    J. Biol. Chem. 284:2482-2492(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BACE1, SUBCELLULAR LOCATION, INDUCTION BY CERAMIDES.
  7. "Molecular identification of NAT8 as the enzyme that acetylates cysteine S-conjugates to mercapturic acids."
    Veiga-da-Cunha M., Tyteca D., Stroobant V., Courtoy P.J., Opperdoes F.R., Van Schaftingen E.
    J. Biol. Chem. 285:18888-18898(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, PATHWAY, CHARACTERIZATION OF VARIANTS LYS-104 AND SER-143, MUTAGENESIS OF ARG-149.
  8. "Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces beta-secretase (BACE1) levels and Abeta generation."
    Ding Y., Ko M.H., Pehar M., Kotch F., Peters N.R., Luo Y., Salamat S.M., Puglielli L.
    J. Biol. Chem. 287:8424-8433(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "Post-translational regulation of CD133 by ATase1/ATase2-mediated lysine acetylation."
    Mak A.B., Pehar M., Nixon A.M., Williams R.A., Uetrecht A.C., Puglielli L., Moffat J.
    J. Mol. Biol. 426:2175-2182(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PROM1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNAT8_HUMAN
AccessioniPrimary (citable) accession number: Q9UHE5
Secondary accession number(s): O75839
, Q6LEU4, Q96QI8, Q9UQ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3