Q9UHB9 (SRP68_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle subunit SRP68
Short name=SRP68

Alternative name(s):
Signal recognition particle 68 kDa protein

Gene names

Name:

SRP68

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Subunit structure	Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Subcellular location	Cytoplasm. Nucleus › nucleolus.
Domain	The RNA-binding domain is located near the N-terminus.
Sequence similarities	Belongs to the SRP68 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Signal recognition particle
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome response to drug Inferred from direct assay PubMed 18089836. Source: UniProtKB translation Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome endoplasmic reticulum Traceable author statement Ref.1. Source: ProtInc nucleolus Traceable author statement Ref.1. Source: ProtInc ribosome Traceable author statement Ref.1. Source: ProtInc signal recognition particle, endoplasmic reticulum targeting Inferred from direct assay PubMed 18089836. Source: UniProtKB
Molecular_function	7S RNA binding Inferred from electronic annotation. Source: InterPro endoplasmic reticulum signal peptide binding Inferred from electronic annotation. Source: InterPro signal recognition particle binding Inferred from physical interaction PubMed 17254600. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UHB9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UHB9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-286: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9UHB9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-339: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 627	627	Signal recognition particle subunit SRP68		PRO_0000135227
Regions
	Compositional bias	9 – 24	16	Poly-Gly
Amino acid modifications
	Modified residue	48	1	Phosphoserine Ref.5
	Modified residue	452	1	N6-acetyllysine Ref.7
Natural variations
	Alternative sequence	1 – 339	339	Missing in isoform 3.		VSP_045132
	Alternative sequence	256 – 286	31	Missing in isoform 2.		VSP_008347
Experimental info
	Sequence conflict	15 – 19	5	Missing in AAF24308. Ref.1
	Sequence conflict	27 – 29	3	Missing in AAF24308. Ref.1
	Sequence conflict	174	1	E → K in AAF24308. Ref.1
	Sequence conflict	534 – 535	2	DA → ES in AAF24308. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: 6EF582892D300B2D	FASTA	627	70,730
	10 20 30 40 50 60 MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL 70 80 90 100 110 120 EILQIIKESQ QQHGLRHGDF QRYRGYCSRR QRRLRKTLNF KMGNRHKFTG KKVTEELLTD 130 140 150 160 170 180 NRYLLLVLMD AERAWSYAMQ LKQEANTEPR KRFHLLSRLR KAVKHAEELE RLCESNRVDA 190 200 210 220 230 240 KTKLEAQAYT AYLSGMLRFE HQEWKAAIEA FNKCKTIYEK LASAFTEEQA VLYNQRVEEI 250 260 270 280 290 300 SPNIRYCAYN IGDQSAINEL MQMRLRSGGT EGLLAEKLEA LITQTRAKQA ATMSEVEWRG 310 320 330 340 350 360 RTVPVKIDKV RIFLLGLADN EAAIVQAESE ETKERLFESM LSECRDAIQV VREELKPDQK 370 380 390 400 410 420 QRDYILEGEP GKVSNLQYLH SYLTYIKLST AIKRNENMAK GLQRALLQQQ PEDDSKRSPR 430 440 450 460 470 480 PQDLIRLYDI ILQNLVELLQ LPGLEEDKAF QKEIGLKTLV FKAYRCFFIA QSYVLVKKWS 490 500 510 520 530 540 EALVLYDRVL KYANEVNSDA GAFKNSLKDL PDVQELITQV RSEKCSLQAA AILDANDAHQ 550 560 570 580 590 600 TETSSSQVKD NKPLVERFET FCLDPSLVTK QANLVHFPPG FQPIPCKPLF FDLALNHVAF 610 620 PPLEDKLEQK TKSGLTGYIK GIFGFRS
	Isoform 2 [UniParc]. Checksum: 025BF536B2452299	FASTA	596	67,304
	10 20 30 40 50 60 MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL 70 80 90 100 110 120 EILQIIKESQ QQHGLRHGDF QRYRGYCSRR QRRLRKTLNF KMGNRHKFTG KKVTEELLTD 130 140 150 160 170 180 NRYLLLVLMD AERAWSYAMQ LKQEANTEPR KRFHLLSRLR KAVKHAEELE RLCESNRVDA 190 200 210 220 230 240 KTKLEAQAYT AYLSGMLRFE HQEWKAAIEA FNKCKTIYEK LASAFTEEQA VLYNQRVEEI 250 260 270 280 290 300 SPNIRYCAYN IGDQSAKQAA TMSEVEWRGR TVPVKIDKVR IFLLGLADNE AAIVQAESEE 310 320 330 340 350 360 TKERLFESML SECRDAIQVV REELKPDQKQ RDYILEGEPG KVSNLQYLHS YLTYIKLSTA 370 380 390 400 410 420 IKRNENMAKG LQRALLQQQP EDDSKRSPRP QDLIRLYDII LQNLVELLQL PGLEEDKAFQ 430 440 450 460 470 480 KEIGLKTLVF KAYRCFFIAQ SYVLVKKWSE ALVLYDRVLK YANEVNSDAG AFKNSLKDLP 490 500 510 520 530 540 DVQELITQVR SEKCSLQAAA ILDANDAHQT ETSSSQVKDN KPLVERFETF CLDPSLVTKQ 550 560 570 580 590 ANLVHFPPGF QPIPCKPLFF DLALNHVAFP PLEDKLEQKT KSGLTGYIKG IFGFRS
	Isoform 3 [UniParc]. Checksum: FF3915ED8952AE0D	FASTA	288	32,851
	10 20 30 40 50 60 MLSECRDAIQ VVREELKPDQ KQRDYILEGE PGKVSNLQYL HSYLTYIKLS TAIKRNENMA 70 80 90 100 110 120 KGLQRALLQQ QPEDDSKRSP RPQDLIRLYD IILQNLVELL QLPGLEEDKA FQKEIGLKTL 130 140 150 160 170 180 VFKAYRCFFI AQSYVLVKKW SEALVLYDRV LKYANEVNSD AGAFKNSLKD LPDVQELITQ 190 200 210 220 230 240 VRSEKCSLQA AAILDANDAH QTETSSSQVK DNKPLVERFE TFCLDPSLVT KQANLVHFPP 250 260 270 280 GFQPIPCKPL FFDLALNHVA FPPLEDKLEQ KTKSGLTGYI KGIFGFRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Signal recognition particle components in the nucleolus." Politz J.C., Yarovoi S., Kilroy S.M., Gowda K., Zwieb C., Pederson T. Proc. Natl. Acad. Sci. U.S.A. 97:55-60(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Mammary gland and Thymus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skin.
[5]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48, MASS SPECTROMETRY. Tissue: Platelet.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-452, MASS SPECTROMETRY.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF195951 mRNA. Translation: AAF24308.1. AK074698 mRNA. Translation: BAC11145.1. AK097962 mRNA. Translation: BAG53557.1. CH471099 Genomic DNA. Translation: EAW89360.1. BC020238 mRNA. Translation: AAH20238.1.
IPI	IPI00102936. IPI00168388.
RefSeq	NP_001247432.1. NM_001260503.1. NP_055045.2. NM_014230.3.
UniGene	Hs.514495.
3D structure databases
ProteinModelPortal	Q9UHB9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UHB9. 4 interactions.
MINT	MINT-3080223.
STRING	9606.ENSP00000312066.
Protein family/group databases
TCDB	3.A.5.9.1. general secretory pathway (Sec) family.
PTM databases
PhosphoSite	Q9UHB9.
Polymorphism databases
DMDM	37154869.
Proteomic databases
PaxDb	Q9UHB9.
PeptideAtlas	Q9UHB9.
PRIDE	Q9UHB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307877; ENSP00000312066; ENSG00000167881.
GeneID	6730.
KEGG	hsa:6730.
UCSC	uc002jqj.1. human. uc002jqk.1. human.
Organism-specific databases
CTD	6730.
GeneCards	GC17M074035.
HGNC	HGNC:11302. SRP68.
HPA	HPA023303.
MIM	604858. gene.
neXtProt	NX_Q9UHB9.
PharmGKB	PA36126.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG325632.
HOGENOM	HOG000231195.
HOVERGEN	HBG059793.
InParanoid	Q9UHB9.
KO	K03107.
OMA	KAAMEAF.
OrthoDB	EOG4V9TQB.
PhylomeDB	Q9UHB9.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9UHB9.
Bgee	Q9UHB9.
CleanEx	HS_SRP68.
Genevestigator	Q9UHB9.
GermOnline	ENSG00000167881. Homo sapiens.
Family and domain databases
InterPro	IPR026258. SRP68. [Graphical view]
PIRSF	PIRSF038995. SRP68. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	Srp68. human.
GenomeRNAi	6730.
NextBio	26254.
SOURCE	Search...

Entry information

Entry name

SRP68_HUMAN

Accession

Primary (citable) accession number: Q9UHB9
Secondary accession number(s): B3KUU5, Q8NCJ4, Q8WUK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	September 26, 2003
Last modified:	May 29, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.