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Q9UHB9 (SRP68_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle subunit SRP68

Short name=SRP68
Alternative name(s):
Signal recognition particle 68 kDa protein
Gene names
Name:SRP68
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length627 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

Subunit structure

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

Subcellular location

Cytoplasm. Nucleusnucleolus.

Domain

The RNA-binding domain is located near the N-terminus.

Sequence similarities

Belongs to the SRP68 family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UHB9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UHB9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-286: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9UHB9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-339: Missing.
Isoform 4 (identifier: Q9UHB9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     84-121: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 627627Signal recognition particle subunit SRP68
PRO_0000135227

Regions

Compositional bias9 – 2416Poly-Gly

Amino acid modifications

Modified residue481Phosphoserine Ref.6
Modified residue4521N6-acetyllysine Ref.8

Natural variations

Alternative sequence1 – 339339Missing in isoform 3.
VSP_045132
Alternative sequence84 – 12138Missing in isoform 4.
VSP_046944
Alternative sequence256 – 28631Missing in isoform 2.
VSP_008347

Experimental info

Sequence conflict15 – 195Missing in AAF24308. Ref.1
Sequence conflict27 – 293Missing in AAF24308. Ref.1
Sequence conflict1741E → K in AAF24308. Ref.1
Sequence conflict534 – 5352DA → ES in AAF24308. Ref.1
Sequence conflict6061K → E in BAG54299. Ref.2

Secondary structure

..................... 627
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 26, 2003. Version 2.
Checksum: 6EF582892D300B2D

FASTA62770,730
        10         20         30         40         50         60 
MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL 

        70         80         90        100        110        120 
EILQIIKESQ QQHGLRHGDF QRYRGYCSRR QRRLRKTLNF KMGNRHKFTG KKVTEELLTD 

       130        140        150        160        170        180 
NRYLLLVLMD AERAWSYAMQ LKQEANTEPR KRFHLLSRLR KAVKHAEELE RLCESNRVDA 

       190        200        210        220        230        240 
KTKLEAQAYT AYLSGMLRFE HQEWKAAIEA FNKCKTIYEK LASAFTEEQA VLYNQRVEEI 

       250        260        270        280        290        300 
SPNIRYCAYN IGDQSAINEL MQMRLRSGGT EGLLAEKLEA LITQTRAKQA ATMSEVEWRG 

       310        320        330        340        350        360 
RTVPVKIDKV RIFLLGLADN EAAIVQAESE ETKERLFESM LSECRDAIQV VREELKPDQK 

       370        380        390        400        410        420 
QRDYILEGEP GKVSNLQYLH SYLTYIKLST AIKRNENMAK GLQRALLQQQ PEDDSKRSPR 

       430        440        450        460        470        480 
PQDLIRLYDI ILQNLVELLQ LPGLEEDKAF QKEIGLKTLV FKAYRCFFIA QSYVLVKKWS 

       490        500        510        520        530        540 
EALVLYDRVL KYANEVNSDA GAFKNSLKDL PDVQELITQV RSEKCSLQAA AILDANDAHQ 

       550        560        570        580        590        600 
TETSSSQVKD NKPLVERFET FCLDPSLVTK QANLVHFPPG FQPIPCKPLF FDLALNHVAF 

       610        620 
PPLEDKLEQK TKSGLTGYIK GIFGFRS 

« Hide

Isoform 2 [UniParc].

Checksum: 025BF536B2452299
Show »

FASTA59667,304
Isoform 3 [UniParc].

Checksum: FF3915ED8952AE0D
Show »

FASTA28832,851
Isoform 4 [UniParc].

Checksum: C8F8D0CB68D9C34D
Show »

FASTA58966,108

References

« Hide 'large scale' references
[1]"Signal recognition particle components in the nucleolus."
Politz J.C., Yarovoi S., Kilroy S.M., Gowda K., Zwieb C., Pederson T.
Proc. Natl. Acad. Sci. U.S.A. 97:55-60(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
Tissue: Liver, Mammary gland and Thymus.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Skin.
[6]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-452, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Web resources

Wikipedia

Signal recognition particle entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF195951 mRNA. Translation: AAF24308.1.
AK074698 mRNA. Translation: BAC11145.1.
AK097962 mRNA. Translation: BAG53557.1.
AK126258 mRNA. Translation: BAG54299.1.
AC040980 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89360.1.
CH471099 Genomic DNA. Translation: EAW89362.1.
BC020238 mRNA. Translation: AAH20238.1.
CCDSCCDS11738.1. [Q9UHB9-1]
CCDS58600.1. [Q9UHB9-3]
CCDS58601.1. [Q9UHB9-4]
RefSeqNP_001247431.1. NM_001260502.1. [Q9UHB9-4]
NP_001247432.1. NM_001260503.1. [Q9UHB9-3]
NP_055045.2. NM_014230.3. [Q9UHB9-1]
UniGeneHs.514495.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4P3EX-ray3.50C47-254[»]
4P3FX-ray1.70A/B47-254[»]
ProteinModelPortalQ9UHB9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112608. 22 interactions.
IntActQ9UHB9. 6 interactions.
MINTMINT-3080223.
STRING9606.ENSP00000312066.

Protein family/group databases

TCDB3.A.5.9.1. the general secretory pathway (sec) family.

PTM databases

PhosphoSiteQ9UHB9.

Polymorphism databases

DMDM37154869.

Proteomic databases

MaxQBQ9UHB9.
PaxDbQ9UHB9.
PeptideAtlasQ9UHB9.
PRIDEQ9UHB9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307877; ENSP00000312066; ENSG00000167881. [Q9UHB9-1]
ENST00000539137; ENSP00000446136; ENSG00000167881. [Q9UHB9-4]
ENST00000602720; ENSP00000473613; ENSG00000167881. [Q9UHB9-3]
GeneID6730.
KEGGhsa:6730.
UCSCuc002jqj.2. human.
uc002jqk.2. human. [Q9UHB9-1]
uc002jql.2. human.

Organism-specific databases

CTD6730.
GeneCardsGC17M074035.
HGNCHGNC:11302. SRP68.
HPAHPA023303.
MIM604858. gene.
neXtProtNX_Q9UHB9.
PharmGKBPA36126.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG325632.
HOGENOMHOG000231195.
HOVERGENHBG059793.
InParanoidQ9UHB9.
KOK03107.
OMAIGDQSAM.
OrthoDBEOG7C5M7W.
PhylomeDBQ9UHB9.
TreeFamTF105779.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9UHB9.
BgeeQ9UHB9.
CleanExHS_SRP68.
GenevestigatorQ9UHB9.

Family and domain databases

InterProIPR026258. SRP68.
[Graphical view]
PIRSFPIRSF038995. SRP68. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSrp68. human.
GenomeRNAi6730.
NextBio26254.
PROQ9UHB9.
SOURCESearch...

Entry information

Entry nameSRP68_HUMAN
AccessionPrimary (citable) accession number: Q9UHB9
Secondary accession number(s): B3KUU5 expand/collapse secondary AC list , B3KWY7, G3V1U4, Q8NCJ4, Q8WUK2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 26, 2003
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM