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Q9UHB9

- SRP68_HUMAN

UniProt

Q9UHB9 - SRP68_HUMAN

Protein

Signal recognition particle subunit SRP68

Gene

SRP68

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (26 Sep 2003)
      Previous versions | rss
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    Functioni

    Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

    GO - Molecular functioni

    1. 7S RNA binding Source: InterPro
    2. endoplasmic reticulum signal peptide binding Source: InterPro
    3. poly(A) RNA binding Source: UniProtKB
    4. signal recognition particle binding Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. gene expression Source: Reactome
    3. response to drug Source: UniProtKB
    4. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
    5. translation Source: Reactome

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_115902. SRP-dependent cotranslational protein targeting to membrane.

    Protein family/group databases

    TCDBi3.A.5.9.1. the general secretory pathway (sec) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal recognition particle subunit SRP68
    Short name:
    SRP68
    Alternative name(s):
    Signal recognition particle 68 kDa protein
    Gene namesi
    Name:SRP68
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:11302. SRP68.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endoplasmic reticulum Source: ProtInc
    3. nucleolus Source: ProtInc
    4. ribosome Source: ProtInc
    5. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Signal recognition particle

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36126.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 627627Signal recognition particle subunit SRP68PRO_0000135227Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481Phosphoserine1 Publication
    Modified residuei452 – 4521N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9UHB9.
    PaxDbiQ9UHB9.
    PeptideAtlasiQ9UHB9.
    PRIDEiQ9UHB9.

    PTM databases

    PhosphoSiteiQ9UHB9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UHB9.
    BgeeiQ9UHB9.
    CleanExiHS_SRP68.
    GenevestigatoriQ9UHB9.

    Organism-specific databases

    HPAiHPA023303.

    Interactioni

    Subunit structurei

    Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

    Protein-protein interaction databases

    BioGridi112608. 22 interactions.
    IntActiQ9UHB9. 6 interactions.
    MINTiMINT-3080223.
    STRINGi9606.ENSP00000312066.

    Structurei

    Secondary structure

    1
    627
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi62 – 7211
    Helixi75 – 773
    Helixi80 – 10324
    Turni104 – 1063
    Beta strandi119 – 1213
    Helixi122 – 14322
    Turni145 – 1473
    Helixi151 – 17222
    Helixi180 – 20021
    Helixi204 – 22219
    Helixi227 – 25125

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4P3EX-ray3.50C47-254[»]
    4P3FX-ray1.70A/B47-254[»]
    ProteinModelPortaliQ9UHB9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi9 – 2416Poly-GlyAdd
    BLAST

    Domaini

    The RNA-binding domain is located near the N-terminus.

    Sequence similaritiesi

    Belongs to the SRP68 family.Curated

    Phylogenomic databases

    eggNOGiNOG325632.
    HOGENOMiHOG000231195.
    HOVERGENiHBG059793.
    InParanoidiQ9UHB9.
    KOiK03107.
    OMAiIGDQSAM.
    OrthoDBiEOG7C5M7W.
    PhylomeDBiQ9UHB9.
    TreeFamiTF105779.

    Family and domain databases

    InterProiIPR026258. SRP68.
    [Graphical view]
    PIRSFiPIRSF038995. SRP68. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UHB9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA    50
    NKEFGDSLSL EILQIIKESQ QQHGLRHGDF QRYRGYCSRR QRRLRKTLNF 100
    KMGNRHKFTG KKVTEELLTD NRYLLLVLMD AERAWSYAMQ LKQEANTEPR 150
    KRFHLLSRLR KAVKHAEELE RLCESNRVDA KTKLEAQAYT AYLSGMLRFE 200
    HQEWKAAIEA FNKCKTIYEK LASAFTEEQA VLYNQRVEEI SPNIRYCAYN 250
    IGDQSAINEL MQMRLRSGGT EGLLAEKLEA LITQTRAKQA ATMSEVEWRG 300
    RTVPVKIDKV RIFLLGLADN EAAIVQAESE ETKERLFESM LSECRDAIQV 350
    VREELKPDQK QRDYILEGEP GKVSNLQYLH SYLTYIKLST AIKRNENMAK 400
    GLQRALLQQQ PEDDSKRSPR PQDLIRLYDI ILQNLVELLQ LPGLEEDKAF 450
    QKEIGLKTLV FKAYRCFFIA QSYVLVKKWS EALVLYDRVL KYANEVNSDA 500
    GAFKNSLKDL PDVQELITQV RSEKCSLQAA AILDANDAHQ TETSSSQVKD 550
    NKPLVERFET FCLDPSLVTK QANLVHFPPG FQPIPCKPLF FDLALNHVAF 600
    PPLEDKLEQK TKSGLTGYIK GIFGFRS 627
    Length:627
    Mass (Da):70,730
    Last modified:September 26, 2003 - v2
    Checksum:i6EF582892D300B2D
    GO
    Isoform 2 (identifier: Q9UHB9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         256-286: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:596
    Mass (Da):67,304
    Checksum:i025BF536B2452299
    GO
    Isoform 3 (identifier: Q9UHB9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-339: Missing.

    Show »
    Length:288
    Mass (Da):32,851
    Checksum:iFF3915ED8952AE0D
    GO
    Isoform 4 (identifier: Q9UHB9-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         84-121: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:589
    Mass (Da):66,108
    Checksum:iC8F8D0CB68D9C34D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 195Missing in AAF24308. (PubMed:10618370)Curated
    Sequence conflicti27 – 293Missing in AAF24308. (PubMed:10618370)Curated
    Sequence conflicti174 – 1741E → K in AAF24308. (PubMed:10618370)Curated
    Sequence conflicti534 – 5352DA → ES in AAF24308. (PubMed:10618370)Curated
    Sequence conflicti606 – 6061K → E in BAG54299. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 339339Missing in isoform 3. 1 PublicationVSP_045132Add
    BLAST
    Alternative sequencei84 – 12138Missing in isoform 4. 1 PublicationVSP_046944Add
    BLAST
    Alternative sequencei256 – 28631Missing in isoform 2. 1 PublicationVSP_008347Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF195951 mRNA. Translation: AAF24308.1.
    AK074698 mRNA. Translation: BAC11145.1.
    AK097962 mRNA. Translation: BAG53557.1.
    AK126258 mRNA. Translation: BAG54299.1.
    AC040980 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89360.1.
    CH471099 Genomic DNA. Translation: EAW89362.1.
    BC020238 mRNA. Translation: AAH20238.1.
    CCDSiCCDS11738.1. [Q9UHB9-1]
    CCDS58600.1. [Q9UHB9-3]
    CCDS58601.1. [Q9UHB9-4]
    RefSeqiNP_001247431.1. NM_001260502.1. [Q9UHB9-4]
    NP_001247432.1. NM_001260503.1. [Q9UHB9-3]
    NP_055045.2. NM_014230.3. [Q9UHB9-1]
    UniGeneiHs.514495.

    Genome annotation databases

    EnsembliENST00000307877; ENSP00000312066; ENSG00000167881. [Q9UHB9-1]
    ENST00000539137; ENSP00000446136; ENSG00000167881. [Q9UHB9-4]
    ENST00000602720; ENSP00000473613; ENSG00000167881. [Q9UHB9-3]
    GeneIDi6730.
    KEGGihsa:6730.
    UCSCiuc002jqj.2. human.
    uc002jqk.2. human. [Q9UHB9-1]
    uc002jql.2. human.

    Polymorphism databases

    DMDMi37154869.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Signal recognition particle entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF195951 mRNA. Translation: AAF24308.1 .
    AK074698 mRNA. Translation: BAC11145.1 .
    AK097962 mRNA. Translation: BAG53557.1 .
    AK126258 mRNA. Translation: BAG54299.1 .
    AC040980 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89360.1 .
    CH471099 Genomic DNA. Translation: EAW89362.1 .
    BC020238 mRNA. Translation: AAH20238.1 .
    CCDSi CCDS11738.1. [Q9UHB9-1 ]
    CCDS58600.1. [Q9UHB9-3 ]
    CCDS58601.1. [Q9UHB9-4 ]
    RefSeqi NP_001247431.1. NM_001260502.1. [Q9UHB9-4 ]
    NP_001247432.1. NM_001260503.1. [Q9UHB9-3 ]
    NP_055045.2. NM_014230.3. [Q9UHB9-1 ]
    UniGenei Hs.514495.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4P3E X-ray 3.50 C 47-254 [» ]
    4P3F X-ray 1.70 A/B 47-254 [» ]
    ProteinModelPortali Q9UHB9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112608. 22 interactions.
    IntActi Q9UHB9. 6 interactions.
    MINTi MINT-3080223.
    STRINGi 9606.ENSP00000312066.

    Protein family/group databases

    TCDBi 3.A.5.9.1. the general secretory pathway (sec) family.

    PTM databases

    PhosphoSitei Q9UHB9.

    Polymorphism databases

    DMDMi 37154869.

    Proteomic databases

    MaxQBi Q9UHB9.
    PaxDbi Q9UHB9.
    PeptideAtlasi Q9UHB9.
    PRIDEi Q9UHB9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307877 ; ENSP00000312066 ; ENSG00000167881 . [Q9UHB9-1 ]
    ENST00000539137 ; ENSP00000446136 ; ENSG00000167881 . [Q9UHB9-4 ]
    ENST00000602720 ; ENSP00000473613 ; ENSG00000167881 . [Q9UHB9-3 ]
    GeneIDi 6730.
    KEGGi hsa:6730.
    UCSCi uc002jqj.2. human.
    uc002jqk.2. human. [Q9UHB9-1 ]
    uc002jql.2. human.

    Organism-specific databases

    CTDi 6730.
    GeneCardsi GC17M074035.
    HGNCi HGNC:11302. SRP68.
    HPAi HPA023303.
    MIMi 604858. gene.
    neXtProti NX_Q9UHB9.
    PharmGKBi PA36126.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG325632.
    HOGENOMi HOG000231195.
    HOVERGENi HBG059793.
    InParanoidi Q9UHB9.
    KOi K03107.
    OMAi IGDQSAM.
    OrthoDBi EOG7C5M7W.
    PhylomeDBi Q9UHB9.
    TreeFami TF105779.

    Enzyme and pathway databases

    Reactomei REACT_115902. SRP-dependent cotranslational protein targeting to membrane.

    Miscellaneous databases

    ChiTaRSi Srp68. human.
    GenomeRNAii 6730.
    NextBioi 26254.
    PROi Q9UHB9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UHB9.
    Bgeei Q9UHB9.
    CleanExi HS_SRP68.
    Genevestigatori Q9UHB9.

    Family and domain databases

    InterProi IPR026258. SRP68.
    [Graphical view ]
    PIRSFi PIRSF038995. SRP68. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
      Tissue: Liver, Mammary gland and Thymus.
    3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Skin.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Platelet.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-452, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSRP68_HUMAN
    AccessioniPrimary (citable) accession number: Q9UHB9
    Secondary accession number(s): B3KUU5
    , B3KWY7, G3V1U4, Q8NCJ4, Q8WUK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: September 26, 2003
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3