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Q9UH92

- MLX_HUMAN

UniProt

Q9UH92 - MLX_HUMAN

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Protein

Max-like protein X

Gene

MLX

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.3 Publications

GO - Molecular functioni

  1. DNA binding Source: BHF-UCL
  2. protein heterodimerization activity Source: BHF-UCL
  3. protein homodimerization activity Source: BHF-UCL
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
  6. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  7. transcription factor binding Source: BHF-UCL

GO - Biological processi

  1. energy reserve metabolic process Source: Reactome
  2. negative regulation of transcription, DNA-templated Source: BHF-UCL
  3. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  4. nucleocytoplasmic transport Source: Ensembl
  5. positive regulation of cellular metabolic process Source: Reactome
  6. regulation of transcription, DNA-templated Source: UniProtKB
  7. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_2122. ChREBP activates metabolic gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Max-like protein X
Alternative name(s):
Class D basic helix-loop-helix protein 13
Short name:
bHLHd13
Max-like bHLHZip protein
Protein BigMax
Transcription factor-like protein 4
Gene namesi
Name:MLX
Synonyms:BHLHD13, TCFL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:11645. MLX.

Subcellular locationi

Isoform Alpha : Cytoplasm
Note: Found predominantly in the cytoplasm.
Isoform Beta : Cytoplasm
Note: Found predominantly in the cytoplasm.
Isoform Gamma : Nucleus
Note: Found predominantly in the nucleus.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

Orphaneti3287. Takayasu arteritis.
PharmGKBiPA36397.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Max-like protein XPRO_0000127279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UH92.
PaxDbiQ9UH92.
PRIDEiQ9UH92.

PTM databases

PhosphoSiteiQ9UH92.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.1 Publication

Gene expression databases

BgeeiQ9UH92.
CleanExiHS_MLX.
ExpressionAtlasiQ9UH92. baseline and differential.
GenevestigatoriQ9UH92.

Organism-specific databases

HPAiCAB025329.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HBZQ2Q0673EBI-741109,EBI-9675545From a different organism.

Protein-protein interaction databases

BioGridi112805. 14 interactions.
IntActiQ9UH92. 8 interactions.
MINTiMINT-1475792.

Structurei

3D structure databases

ProteinModelPortaliQ9UH92.
SMRiQ9UH92. Positions 125-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 18759bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 16021Leucine-zipperAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG259028.
GeneTreeiENSGT00530000063219.
HOVERGENiHBG019061.
InParanoidiQ9UH92.
KOiK09113.
OMAiPHILRNI.
OrthoDBiEOG73805M.
PhylomeDBiQ9UH92.
TreeFamiTF318841.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Gamma (identifier: Q9UH92-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKASPVGA HAGEGRAGRA RARRGAGRRG ASLLSPKSPT
60 70 80 90 100
LSVPRGCRED SSHPACAKVE YAYSDNSLDP GLFVESTRKG SVVSRANSIG
110 120 130 140 150
STSASSVPNT DDEDSDYHQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD
160 170 180 190 200
DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST
210 220 230 240 250
LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF
260 270 280 290
QSFNASISVA SFQELSACVF SWIEEHCKPQ TLREIVIGVL HQLKNQLY
Length:298
Mass (Da):33,300
Last modified:October 1, 2001 - v2
Checksum:iD1981730051473C5
GO
Isoform Alpha (identifier: Q9UH92-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.
     81-110: Missing.

Show »
Length:214
Mass (Da):24,905
Checksum:i025F90C6F253E56D
GO
Isoform Beta (identifier: Q9UH92-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.

Show »
Length:244
Mass (Da):27,883
Checksum:i63EED502A9FA082D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti247 – 2471D → V in AAF14638. (PubMed:10593926)Curated
Sequence conflicti298 – 2981Y → C in BAA90977. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti223 – 2231Q → R.
Corresponds to variant rs665268 [ dbSNP | Ensembl ].
VAR_049547

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 6854Missing in isoform Alpha and isoform Beta. 5 PublicationsVSP_002137Add
BLAST
Alternative sequencei81 – 11030Missing in isoform Alpha. 2 PublicationsVSP_002138Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203978 mRNA. Translation: AAF14638.1.
AF213666 mRNA. Translation: AAG40145.1.
AF213667 mRNA. Translation: AAG40146.1.
AF213668 mRNA. Translation: AAG40147.1.
AK000150 mRNA. Translation: BAA90977.1.
AK290258 mRNA. Translation: BAF82947.1.
AK314378 mRNA. Translation: BAG37005.1.
AK315432 mRNA. Translation: BAG37820.1.
BT009812 mRNA. Translation: AAP88814.1.
BC010689 mRNA. Translation: AAH10689.1.
CCDSiCCDS11430.1. [Q9UH92-1]
CCDS42341.1. [Q9UH92-2]
CCDS45687.1. [Q9UH92-3]
PIRiJC5333.
RefSeqiNP_733752.1. NM_170607.2. [Q9UH92-1]
NP_937847.1. NM_198204.1. [Q9UH92-3]
NP_937848.1. NM_198205.1. [Q9UH92-2]
UniGeneiHs.383019.
Hs.714749.

Genome annotation databases

EnsembliENST00000246912; ENSP00000246912; ENSG00000108788. [Q9UH92-1]
ENST00000346833; ENSP00000320913; ENSG00000108788. [Q9UH92-2]
ENST00000435881; ENSP00000416627; ENSG00000108788. [Q9UH92-3]
GeneIDi6945.
KEGGihsa:6945.
UCSCiuc002iaf.3. human. [Q9UH92-3]
uc002iag.3. human. [Q9UH92-1]
uc002iah.3. human. [Q9UH92-2]

Polymorphism databases

DMDMi20138856.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203978 mRNA. Translation: AAF14638.1 .
AF213666 mRNA. Translation: AAG40145.1 .
AF213667 mRNA. Translation: AAG40146.1 .
AF213668 mRNA. Translation: AAG40147.1 .
AK000150 mRNA. Translation: BAA90977.1 .
AK290258 mRNA. Translation: BAF82947.1 .
AK314378 mRNA. Translation: BAG37005.1 .
AK315432 mRNA. Translation: BAG37820.1 .
BT009812 mRNA. Translation: AAP88814.1 .
BC010689 mRNA. Translation: AAH10689.1 .
CCDSi CCDS11430.1. [Q9UH92-1 ]
CCDS42341.1. [Q9UH92-2 ]
CCDS45687.1. [Q9UH92-3 ]
PIRi JC5333.
RefSeqi NP_733752.1. NM_170607.2. [Q9UH92-1 ]
NP_937847.1. NM_198204.1. [Q9UH92-3 ]
NP_937848.1. NM_198205.1. [Q9UH92-2 ]
UniGenei Hs.383019.
Hs.714749.

3D structure databases

ProteinModelPortali Q9UH92.
SMRi Q9UH92. Positions 125-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112805. 14 interactions.
IntActi Q9UH92. 8 interactions.
MINTi MINT-1475792.

Chemistry

BindingDBi Q9UH92.
ChEMBLi CHEMBL2062357.

PTM databases

PhosphoSitei Q9UH92.

Polymorphism databases

DMDMi 20138856.

Proteomic databases

MaxQBi Q9UH92.
PaxDbi Q9UH92.
PRIDEi Q9UH92.

Protocols and materials databases

DNASUi 6945.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000246912 ; ENSP00000246912 ; ENSG00000108788 . [Q9UH92-1 ]
ENST00000346833 ; ENSP00000320913 ; ENSG00000108788 . [Q9UH92-2 ]
ENST00000435881 ; ENSP00000416627 ; ENSG00000108788 . [Q9UH92-3 ]
GeneIDi 6945.
KEGGi hsa:6945.
UCSCi uc002iaf.3. human. [Q9UH92-3 ]
uc002iag.3. human. [Q9UH92-1 ]
uc002iah.3. human. [Q9UH92-2 ]

Organism-specific databases

CTDi 6945.
GeneCardsi GC17P040719.
HGNCi HGNC:11645. MLX.
HPAi CAB025329.
MIMi 602976. gene.
neXtProti NX_Q9UH92.
Orphaneti 3287. Takayasu arteritis.
PharmGKBi PA36397.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG259028.
GeneTreei ENSGT00530000063219.
HOVERGENi HBG019061.
InParanoidi Q9UH92.
KOi K09113.
OMAi PHILRNI.
OrthoDBi EOG73805M.
PhylomeDBi Q9UH92.
TreeFami TF318841.

Enzyme and pathway databases

Reactomei REACT_2122. ChREBP activates metabolic gene expression.

Miscellaneous databases

ChiTaRSi MLX. human.
GeneWikii MLX_(gene).
GenomeRNAii 6945.
NextBioi 27189.
PROi Q9UH92.
SOURCEi Search...

Gene expression databases

Bgeei Q9UH92.
CleanExi HS_MLX.
ExpressionAtlasi Q9UH92. baseline and differential.
Genevestigatori Q9UH92.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mlx, a novel Max-like bHLHZip protein that interacts with the Max network of transcription factors."
    Billin A.N., Eilers A.L., Queva C., Ayer D.E.
    J. Biol. Chem. 274:36344-36350(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
    Tissue: Promyelocyte.
  2. "Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?"
    Meroni G., Cairo S., Merla G., Messali S., Brent R., Ballabio A., Reymond A.
    Oncogene 19:3266-3277(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA), SUBCELLULAR LOCATION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ALPHA; BETA AND GAMMA).
    Tissue: Brain and Colon.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
    Tissue: Eye.
  6. "A novel heterodimerization domain, CRM1, and 14-3-3 control subcellular localization of the MondoA-Mlx heterocomplex."
    Eilers A.L., Sundwall E., Lin M., Sullivan A.A., Ayer D.E.
    Mol. Cell. Biol. 22:8514-8526(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MLXIP.
  7. "MondoA-Mlx heterodimers are candidate sensors of cellular energy status: mitochondrial localization and direct regulation of glycolysis."
    Sans C.L., Satterwhite D.J., Stoltzman C.A., Breen K.T., Ayer D.E.
    Mol. Cell. Biol. 26:4863-4871(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMLX_HUMAN
AccessioniPrimary (citable) accession number: Q9UH92
Secondary accession number(s): A8K2J3
, B2RAV8, B2RD73, Q53XM6, Q96FL2, Q9H2V0, Q9H2V1, Q9H2V2, Q9NXN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2001
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3