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Protein

Max-like protein X

Gene

MLX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.3 Publications

GO - Molecular functioni

  • DNA binding Source: BHF-UCL
  • DNA binding transcription factor activity Source: UniProtKB
  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcription factor binding Source: BHF-UCL

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: BHF-UCL
  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-163765 ChREBP activates metabolic gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Max-like protein X
Alternative name(s):
Class D basic helix-loop-helix protein 13
Short name:
bHLHd13
Max-like bHLHZip protein
Protein BigMax
Transcription factor-like protein 4
Gene namesi
Name:MLX
Synonyms:BHLHD13, TCFL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108788.11
HGNCiHGNC:11645 MLX
MIMi602976 gene
neXtProtiNX_Q9UH92

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6945
MalaCardsiMLX
OpenTargetsiENSG00000108788
Orphaneti3287 Takayasu arteritis
PharmGKBiPA36397

Chemistry databases

ChEMBLiCHEMBL2062357

Polymorphism and mutation databases

BioMutaiMLX
DMDMi20138856

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272791 – 298Max-like protein XAdd BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineCombined sources1
Modified residuei45PhosphoserineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei74PhosphoserineCombined sources1
Modified residuei77PhosphoserineCombined sources1
Modified residuei98PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UH92
PaxDbiQ9UH92
PeptideAtlasiQ9UH92
PRIDEiQ9UH92
ProteomicsDBi84286
84287 [Q9UH92-2]
84288 [Q9UH92-3]

PTM databases

iPTMnetiQ9UH92
PhosphoSitePlusiQ9UH92

Expressioni

Tissue specificityi

Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.1 Publication

Gene expression databases

BgeeiENSG00000108788
CleanExiHS_MLX
ExpressionAtlasiQ9UH92 baseline and differential
GenevisibleiQ9UH92 HS

Organism-specific databases

HPAiCAB025329
HPA052766
HPA064438

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi112805, 27 interactors
IntActiQ9UH92, 22 interactors
MINTiQ9UH92
STRINGi9606.ENSP00000246912

Chemistry databases

BindingDBiQ9UH92

Structurei

3D structure databases

ProteinModelPortaliQ9UH92
SMRiQ9UH92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 187bHLHPROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 160Leucine-zipperAdd BLAST21

Phylogenomic databases

eggNOGiKOG1319 Eukaryota
ENOG410ZWIP LUCA
GeneTreeiENSGT00530000063219
HOVERGENiHBG019061
InParanoidiQ9UH92
KOiK09113
OMAiIMEEMFE
OrthoDBiEOG091G0RUK
PhylomeDBiQ9UH92
TreeFamiTF318841

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR032647 Mlx
PANTHERiPTHR15741:SF25 PTHR15741:SF25, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma (identifier: Q9UH92-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKASPVGA HAGEGRAGRA RARRGAGRRG ASLLSPKSPT
60 70 80 90 100
LSVPRGCRED SSHPACAKVE YAYSDNSLDP GLFVESTRKG SVVSRANSIG
110 120 130 140 150
STSASSVPNT DDEDSDYHQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD
160 170 180 190 200
DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST
210 220 230 240 250
LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF
260 270 280 290
QSFNASISVA SFQELSACVF SWIEEHCKPQ TLREIVIGVL HQLKNQLY
Length:298
Mass (Da):33,300
Last modified:October 1, 2001 - v2
Checksum:iD1981730051473C5
GO
Isoform Alpha (identifier: Q9UH92-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.
     81-110: Missing.

Show »
Length:214
Mass (Da):24,905
Checksum:i025F90C6F253E56D
GO
Isoform Beta (identifier: Q9UH92-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-68: Missing.

Show »
Length:244
Mass (Da):27,883
Checksum:i63EED502A9FA082D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247D → V in AAF14638 (PubMed:10593926).Curated1
Sequence conflicti298Y → C in BAA90977 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049547223Q → R. Corresponds to variant dbSNP:rs665268Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00213715 – 68Missing in isoform Alpha and isoform Beta. 5 PublicationsAdd BLAST54
Alternative sequenceiVSP_00213881 – 110Missing in isoform Alpha. 2 PublicationsAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF203978 mRNA Translation: AAF14638.1
AF213666 mRNA Translation: AAG40145.1
AF213667 mRNA Translation: AAG40146.1
AF213668 mRNA Translation: AAG40147.1
AK000150 mRNA Translation: BAA90977.1
AK290258 mRNA Translation: BAF82947.1
AK314378 mRNA Translation: BAG37005.1
AK315432 mRNA Translation: BAG37820.1
BT009812 mRNA Translation: AAP88814.1
BC010689 mRNA Translation: AAH10689.1
CCDSiCCDS11430.1 [Q9UH92-1]
CCDS42341.1 [Q9UH92-2]
CCDS45687.1 [Q9UH92-3]
PIRiJC5333
RefSeqiNP_733752.1, NM_170607.2 [Q9UH92-1]
NP_937847.1, NM_198204.1 [Q9UH92-3]
NP_937848.1, NM_198205.1 [Q9UH92-2]
UniGeneiHs.383019
Hs.714749

Genome annotation databases

EnsembliENST00000246912; ENSP00000246912; ENSG00000108788 [Q9UH92-1]
ENST00000346833; ENSP00000320913; ENSG00000108788 [Q9UH92-2]
ENST00000435881; ENSP00000416627; ENSG00000108788 [Q9UH92-3]
GeneIDi6945
KEGGihsa:6945
UCSCiuc002iaf.4 human [Q9UH92-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMLX_HUMAN
AccessioniPrimary (citable) accession number: Q9UH92
Secondary accession number(s): A8K2J3
, B2RAV8, B2RD73, Q53XM6, Q96FL2, Q9H2V0, Q9H2V1, Q9H2V2, Q9NXN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2001
Last modified: June 20, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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