Q9UH73 (COE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor COE1
Short name=O/E-1
Short name=OE-1

Alternative name(s):
Early B-cell factor

Gene names

Name:	EBF1
Synonyms:	COE1, EBF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' By similarity.
Subunit structure	Forms either a homodimer or a heterodimer with a related family member By similarity. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes. Ref.3
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the COE family. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UH73-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UH73-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.
     252-259: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 591

591

Transcription factor COE1

PRO_0000107825

Regions

Domain

262 – 345

84

IPT/TIG

Zinc finger

151 – 170

20

C5-type Potential

Region

63 – 66

4

Interaction with DNA By similarity

Region

197 – 204

8

Interaction with DNA By similarity

Region

236 – 239

4

Interaction with DNA By similarity

Compositional bias

462 – 550

89

Pro/Ser/Thr-rich

Sites

Site

163

1

Interaction with DNA By similarity

Site

172

1

Interaction with DNA By similarity

Natural variations

Alternative sequence

162 – 184

23

Missing in isoform 2.

VSP_012304

Alternative sequence

252 – 259

8

Missing in isoform 2.

VSP_012305

Experimental info

Sequence conflict

112

1

L → I in AAF19643. Ref.2

Sequence conflict

147

1

N → S in AAF19643. Ref.2

Sequence conflict

228 – 229

2

VS → AP in AAF19643. Ref.2

Sequence conflict

305 – 306

2

IT → TG in AAF19643. Ref.2

Sequence conflict

309

1

A → S in AAF19643. Ref.2

Sequence conflict

347

1

L → R in AAF19643. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

591

Helix Strand Turn

Details...

Beta strand

266 – 268

3

Beta strand

279 – 283

5

Beta strand

292 – 294

3

Beta strand

302 – 306

5

Beta strand

309 – 313

5

Beta strand

326 – 329

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: E47797B6FC1E5071	FASTA	591	64,464
	10 20 30 40 50 60 MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA RAHFEKQPPS 70 80 90 100 110 120 NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN SEKTNNGIHY RLQLLYSNGI 130 140 150 160 170 180 RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CSRCCDKKSC GNRNETPSDP 190 200 210 220 230 240 VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH 250 260 270 280 290 300 GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV 310 320 330 340 350 360 WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ 370 380 390 400 410 420 KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ 430 440 450 460 470 480 LPALANTSVH AGMMGVNSFS GQLAVNVSEA SQATNQGFTR NSSSVSPHGY VPSTTPQQTN 490 500 510 520 530 540 YNSVTTSMNG YGSAAMSNLG GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI 550 560 570 580 590 FSFSPANMVS AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M
	Isoform 2 [UniParc]. Checksum: 5D199E23BDD2E2F6	FASTA	560	61,059
	10 20 30 40 50 60 MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA RAHFEKQPPS 70 80 90 100 110 120 NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN SEKTNNGIHY RLQLLYSNGI 130 140 150 160 170 180 RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM CRVLLTHEIM CRFFLKFFLK CNQNCLKNAG 190 200 210 220 230 240 NPRDMRRFQV VVSTTVNVDG HVLAVSDNMF VHNNSKHGRR ARRLDPSEAT PCIKAISPSE 250 260 270 280 290 300 GWTTGGATVI IIGDNFFDGL QVIFGTMLVW SELITPHAIR VQTPPRHIPG VVEVTLSYKS 310 320 330 340 350 360 KQFCKGTPGR FIYTALNEPT IDYGFQRLQK VIPRHPGDPE RLPKEVILKR AADLVEALYG 370 380 390 400 410 420 MPHNNQEIIL KRAADIAEAL YSVPRNHNQL PALANTSVHA GMMGVNSFSG QLAVNVSEAS 430 440 450 460 470 480 QATNQGFTRN SSSVSPHGYV PSTTPQQTNY NSVTTSMNGY GSAAMSNLGG SPTFLNGSAA 490 500 510 520 530 540 NSPYAIVPSS PTMASSTSLP SNCSSSSGIF SFSPANMVSA VKQKSAFAPV VRPQTSPPPT 550 560 CTSTNGNSLQ AISGMIVPPM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[2]	"Cloning of human early B-cell factor and identification of target genes suggest a conserved role in B-cell development in man and mouse." Gisler R., Jacobsen S.E., Sigvardsson M. Blood 96:1457-1464(2000) [PubMed: 10942392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-591 (ISOFORM 1).
[3]	"Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells." Bond H.M., Mesuraca M., Carbone E., Bonelli P., Agosti V., Amodio N., De Rosa G., Di Nicola M., Gianni A.M., Moore M.A., Hata A., Grieco M., Morrone G., Venuta S. Blood 103:2062-2070(2004) [PubMed: 14630787] [Abstract] Cited for: INTERACTION WITH ZNF521.
[4]	"Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA-binding proteins and a novel dimerization motif." Siponen M.I., Wisniewska M., Lehtio L., Johansson I., Svensson L., Raszewski G., Nilsson L., Sigvardsson M., Berglund H. J. Biol. Chem. 285:25875-25879(2010) [PubMed: 20592035] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 10-351 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC038805 mRNA. Translation: AAH38805.1.
BC041178 mRNA. Translation: AAH41178.1.
AF208502 mRNA. Translation: AAF19643.1.

IPI

IPI00329425.
IPI00383432.

RefSeq

NP_076870.1. NM_024007.3.

UniGene

Hs.573143.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LYR	X-ray	2.51	A	10-250	[»]
3MQI	X-ray	2.30	A/B/C	258-351	[»]

ProteinModelPortal

Q9UH73.

SMR

Q9UH73. Positions 35-413.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UH73. 1 interaction.

STRING

Q9UH73.

Polymorphism databases

DMDM

47117917.

Proteomic databases

PRIDE

Q9UH73.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000313708; ENSP00000322898; ENSG00000164330.

GeneID

1879.

KEGG

hsa:1879.

UCSC

uc003lxl.2. human.
uc010jip.1. human.

Organism-specific databases

CTD

1879.

GeneCards

GC05M158059.

H-InvDB

HIX0005366.

HGNC

HGNC:3126. EBF1.

MIM

164343. gene.

neXtProt

NX_Q9UH73.

PharmGKB

PA162384225.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09086.

GeneTree

ENSGT00390000014051.

HOGENOM

HBG444229.

HOVERGEN

HBG005108.

InParanoid

Q9UH73.

OMA

YANSGMA.

OrthoDB

EOG4TXBRK.

PhylomeDB

Q9UH73.

Enzyme and pathway databases

Reactome

REACT_111045. Developmental Biology.

Gene expression databases

ArrayExpress

Q9UH73.

Bgee

Q9UH73.

CleanEx

HS_EBF1.

Genevestigator

Q9UH73.

GermOnline

ENSG00000164330. Homo sapiens.

Family and domain databases

InterPro

IPR011598. HLH_DNA-bd.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_TIG_rcpt.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

KO

K09103.

PANTHER

PTHR10747. TF_COE. 1 hit.

Pfam

PF01833. TIG. 1 hit.
[Graphical view]

SMART

SM00353. HLH. 1 hit.
SM00429. IPT. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

PROSITE

PS01345. COE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

7685.

SOURCE

Search...

Entry information

Entry name

COE1_HUMAN

Accession

Primary (citable) accession number: Q9UH73
Secondary accession number(s): Q8IW11

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.