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Protein

Transcription factor COE1

Gene

EBF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri151 – 170C5-typeSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164330-MONOMER.
ReactomeiR-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SIGNORiQ9UH73.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor COE1
Short name:
O/E-1
Short name:
OE-1
Alternative name(s):
Early B-cell factor
Gene namesi
Name:EBF1
Synonyms:COE1, EBF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3126. EBF1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1879.
OpenTargetsiENSG00000164330.
PharmGKBiPA162384225.

Polymorphism and mutation databases

BioMutaiEBF1.
DMDMi47117917.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001078251 – 591Transcription factor COE1Add BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9UH73.
PeptideAtlasiQ9UH73.
PRIDEiQ9UH73.

PTM databases

iPTMnetiQ9UH73.
PhosphoSitePlusiQ9UH73.
SwissPalmiQ9UH73.

Expressioni

Gene expression databases

BgeeiENSG00000164330.
CleanExiHS_EBF1.
ExpressionAtlasiQ9UH73. baseline and differential.
GenevisibleiQ9UH73. HS.

Interactioni

Subunit structurei

Forms either a homodimer or a heterodimer with a related family member (By similarity). Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei163Interaction with DNABy similarity1
Sitei172Interaction with DNABy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
ATPAF2Q8N5M13EBI-765426,EBI-1166928
HOMEZQ8IX15-33EBI-765426,EBI-10172004

Protein-protein interaction databases

BioGridi108211. 8 interactors.
IntActiQ9UH73. 6 interactors.
STRINGi9606.ENSP00000322898.

Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 31Combined sources7
Beta strandi36 – 38Combined sources3
Helixi39 – 43Combined sources5
Turni44 – 46Combined sources3
Beta strandi49 – 56Combined sources8
Beta strandi60 – 63Combined sources4
Beta strandi66 – 76Combined sources11
Beta strandi83 – 93Combined sources11
Helixi95 – 97Combined sources3
Helixi99 – 101Combined sources3
Beta strandi108 – 115Combined sources8
Beta strandi121 – 132Combined sources12
Turni133 – 135Combined sources3
Beta strandi152 – 155Combined sources4
Helixi158 – 160Combined sources3
Helixi162 – 165Combined sources4
Turni171 – 175Combined sources5
Beta strandi181 – 183Combined sources3
Turni184 – 186Combined sources3
Beta strandi187 – 194Combined sources8
Beta strandi211 – 219Combined sources9
Beta strandi223 – 228Combined sources6
Beta strandi232 – 235Combined sources4
Beta strandi263 – 268Combined sources6
Beta strandi271 – 273Combined sources3
Beta strandi279 – 286Combined sources8
Beta strandi292 – 295Combined sources4
Beta strandi298 – 300Combined sources3
Beta strandi302 – 306Combined sources5
Beta strandi309 – 313Combined sources5
Beta strandi321 – 329Combined sources9
Beta strandi340 – 345Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LYRX-ray2.51A10-250[»]
3MQIX-ray2.30A/B/C258-351[»]
ProteinModelPortaliQ9UH73.
SMRiQ9UH73.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UH73.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 345IPT/TIGAdd BLAST84

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 66Interaction with DNABy similarity4
Regioni197 – 204Interaction with DNABy similarity8
Regioni236 – 239Interaction with DNABy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi462 – 550Pro/Ser/Thr-richAdd BLAST89

Sequence similaritiesi

Belongs to the COE family.Curated
Contains 1 IPT/TIG domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri151 – 170C5-typeSequence analysisAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3836. Eukaryota.
ENOG410XQ9Z. LUCA.
GeneTreeiENSGT00390000014051.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
InParanoidiQ9UH73.
KOiK09103.
OMAiHAATPCI.
OrthoDBiEOG091G0A9D.
PhylomeDBiQ9UH73.
TreeFamiTF313391.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR032200. COE_DBD.
IPR032201. COE_HLH.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF16422. COE1_DBD. 1 hit.
PF16423. COE1_HLH. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UH73-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA
60 70 80 90 100
RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN
110 120 130 140 150
SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM
160 170 180 190 200
CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK
210 220 230 240 250
NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS
260 270 280 290 300
EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV
310 320 330 340 350
WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP
360 370 380 390 400
TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII
410 420 430 440 450
LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA
460 470 480 490 500
SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG
510 520 530 540 550
GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS
560 570 580 590
AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M
Length:591
Mass (Da):64,464
Last modified:May 10, 2004 - v2
Checksum:iE47797B6FC1E5071
GO
Isoform 2 (identifier: Q9UH73-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     162-184: Missing.
     252-259: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):61,059
Checksum:i5D199E23BDD2E2F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112L → I in AAF19643 (PubMed:10942392).Curated1
Sequence conflicti147N → S in AAF19643 (PubMed:10942392).Curated1
Sequence conflicti228 – 229VS → AP in AAF19643 (PubMed:10942392).Curated2
Sequence conflicti305 – 306IT → TG in AAF19643 (PubMed:10942392).Curated2
Sequence conflicti309A → S in AAF19643 (PubMed:10942392).Curated1
Sequence conflicti347L → R in AAF19643 (PubMed:10942392).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012304162 – 184Missing in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_012305252 – 259Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC038805 mRNA. Translation: AAH38805.1.
BC041178 mRNA. Translation: AAH41178.1.
AF208502 mRNA. Translation: AAF19643.1.
CCDSiCCDS4343.1. [Q9UH73-1]
CCDS78081.1. [Q9UH73-2]
RefSeqiNP_076870.1. NM_024007.4. [Q9UH73-1]
NP_874367.1. NM_182708.2. [Q9UH73-2]
UniGeneiHs.573143.
Hs.733761.

Genome annotation databases

EnsembliENST00000313708; ENSP00000322898; ENSG00000164330. [Q9UH73-1]
ENST00000380654; ENSP00000370029; ENSG00000164330. [Q9UH73-2]
GeneIDi1879.
KEGGihsa:1879.
UCSCiuc003lxl.7. human. [Q9UH73-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC038805 mRNA. Translation: AAH38805.1.
BC041178 mRNA. Translation: AAH41178.1.
AF208502 mRNA. Translation: AAF19643.1.
CCDSiCCDS4343.1. [Q9UH73-1]
CCDS78081.1. [Q9UH73-2]
RefSeqiNP_076870.1. NM_024007.4. [Q9UH73-1]
NP_874367.1. NM_182708.2. [Q9UH73-2]
UniGeneiHs.573143.
Hs.733761.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LYRX-ray2.51A10-250[»]
3MQIX-ray2.30A/B/C258-351[»]
ProteinModelPortaliQ9UH73.
SMRiQ9UH73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108211. 8 interactors.
IntActiQ9UH73. 6 interactors.
STRINGi9606.ENSP00000322898.

PTM databases

iPTMnetiQ9UH73.
PhosphoSitePlusiQ9UH73.
SwissPalmiQ9UH73.

Polymorphism and mutation databases

BioMutaiEBF1.
DMDMi47117917.

Proteomic databases

PaxDbiQ9UH73.
PeptideAtlasiQ9UH73.
PRIDEiQ9UH73.

Protocols and materials databases

DNASUi1879.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313708; ENSP00000322898; ENSG00000164330. [Q9UH73-1]
ENST00000380654; ENSP00000370029; ENSG00000164330. [Q9UH73-2]
GeneIDi1879.
KEGGihsa:1879.
UCSCiuc003lxl.7. human. [Q9UH73-1]

Organism-specific databases

CTDi1879.
DisGeNETi1879.
GeneCardsiEBF1.
HGNCiHGNC:3126. EBF1.
MIMi164343. gene.
neXtProtiNX_Q9UH73.
OpenTargetsiENSG00000164330.
PharmGKBiPA162384225.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3836. Eukaryota.
ENOG410XQ9Z. LUCA.
GeneTreeiENSGT00390000014051.
HOGENOMiHOG000092311.
HOVERGENiHBG005108.
InParanoidiQ9UH73.
KOiK09103.
OMAiHAATPCI.
OrthoDBiEOG091G0A9D.
PhylomeDBiQ9UH73.
TreeFamiTF313391.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164330-MONOMER.
ReactomeiR-HSA-381340. Transcriptional regulation of white adipocyte differentiation.
SIGNORiQ9UH73.

Miscellaneous databases

ChiTaRSiEBF1. human.
EvolutionaryTraceiQ9UH73.
GeneWikiiEBF1.
GenomeRNAii1879.
PROiQ9UH73.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164330.
CleanExiHS_EBF1.
ExpressionAtlasiQ9UH73. baseline and differential.
GenevisibleiQ9UH73. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR032200. COE_DBD.
IPR032201. COE_HLH.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR003523. Transcription_factor_COE.
IPR018350. Transcription_factor_COE_CS.
[Graphical view]
PANTHERiPTHR10747. PTHR10747. 1 hit.
PfamiPF16422. COE1_DBD. 1 hit.
PF16423. COE1_HLH. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiPS01345. COE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOE1_HUMAN
AccessioniPrimary (citable) accession number: Q9UH73
Secondary accession number(s): Q8IW11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: May 10, 2004
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.