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Q9UH73

- COE1_HUMAN

UniProt

Q9UH73 - COE1_HUMAN

Protein

Transcription factor COE1

Gene

EBF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (10 May 2004)
      Previous versions | rss
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    Functioni

    Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei163 – 1631Interaction with DNABy similarity
    Sitei172 – 1721Interaction with DNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: Ensembl

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. positive regulation of transcription, DNA-templated Source: Ensembl
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor COE1
    Short name:
    O/E-1
    Short name:
    OE-1
    Alternative name(s):
    Early B-cell factor
    Gene namesi
    Name:EBF1
    Synonyms:COE1, EBF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:3126. EBF1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162384225.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 591591Transcription factor COE1PRO_0000107825Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9UH73.
    PaxDbiQ9UH73.
    PRIDEiQ9UH73.

    PTM databases

    PhosphoSiteiQ9UH73.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UH73.
    BgeeiQ9UH73.
    CleanExiHS_EBF1.
    GenevestigatoriQ9UH73.

    Interactioni

    Subunit structurei

    Forms either a homodimer or a heterodimer with a related family member By similarity. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi108211. 4 interactions.
    IntActiQ9UH73. 1 interaction.
    STRINGi9606.ENSP00000322898.

    Structurei

    Secondary structure

    1
    591
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 317
    Beta strandi36 – 383
    Helixi39 – 435
    Turni44 – 463
    Beta strandi49 – 568
    Beta strandi60 – 634
    Beta strandi66 – 7611
    Beta strandi83 – 9311
    Helixi95 – 973
    Helixi99 – 1013
    Beta strandi108 – 1158
    Beta strandi121 – 13212
    Turni133 – 1353
    Beta strandi152 – 1554
    Helixi158 – 1603
    Helixi162 – 1654
    Turni171 – 1755
    Beta strandi181 – 1833
    Turni184 – 1863
    Beta strandi187 – 1948
    Beta strandi211 – 2199
    Beta strandi223 – 2286
    Beta strandi232 – 2354
    Beta strandi263 – 2686
    Beta strandi271 – 2733
    Beta strandi279 – 2868
    Beta strandi292 – 2954
    Beta strandi298 – 3003
    Beta strandi302 – 3065
    Beta strandi309 – 3135
    Beta strandi321 – 3299
    Beta strandi340 – 3456

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LYRX-ray2.51A10-250[»]
    3MQIX-ray2.30A/B/C258-351[»]
    ProteinModelPortaliQ9UH73.
    SMRiQ9UH73. Positions 35-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UH73.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini262 – 34584IPT/TIGAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 664Interaction with DNABy similarity
    Regioni197 – 2048Interaction with DNABy similarity
    Regioni236 – 2394Interaction with DNABy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi462 – 55089Pro/Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the COE family.Curated
    Contains 1 IPT/TIG domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri151 – 17020C5-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG259050.
    HOGENOMiHOG000092311.
    HOVERGENiHBG005108.
    InParanoidiQ9UH73.
    KOiK09103.
    OMAiHAATPCI.
    OrthoDBiEOG7J446V.
    PhylomeDBiQ9UH73.
    TreeFamiTF313391.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view]
    PANTHERiPTHR10747. PTHR10747. 1 hit.
    PfamiPF01833. TIG. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.
    PROSITEiPS01345. COE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UH73-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFGIQESIQR SGSSMKEEPL GSGMNAVRTW MQGAGVLDAN TAAQSGVGLA    50
    RAHFEKQPPS NLRKSNFFHF VLALYDRQGQ PVEIERTAFV GFVEKEKEAN 100
    SEKTNNGIHY RLQLLYSNGI RTEQDFYVRL IDSMTKQAIV YEGQDKNPEM 150
    CRVLLTHEIM CSRCCDKKSC GNRNETPSDP VIIDRFFLKF FLKCNQNCLK 200
    NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS 250
    EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV 300
    WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP 350
    TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII 400
    LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA 450
    SQATNQGFTR NSSSVSPHGY VPSTTPQQTN YNSVTTSMNG YGSAAMSNLG 500
    GSPTFLNGSA ANSPYAIVPS SPTMASSTSL PSNCSSSSGI FSFSPANMVS 550
    AVKQKSAFAP VVRPQTSPPP TCTSTNGNSL QAISGMIVPP M 591
    Length:591
    Mass (Da):64,464
    Last modified:May 10, 2004 - v2
    Checksum:iE47797B6FC1E5071
    GO
    Isoform 2 (identifier: Q9UH73-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         162-184: Missing.
         252-259: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:560
    Mass (Da):61,059
    Checksum:i5D199E23BDD2E2F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121L → I in AAF19643. (PubMed:10942392)Curated
    Sequence conflicti147 – 1471N → S in AAF19643. (PubMed:10942392)Curated
    Sequence conflicti228 – 2292VS → AP in AAF19643. (PubMed:10942392)Curated
    Sequence conflicti305 – 3062IT → TG in AAF19643. (PubMed:10942392)Curated
    Sequence conflicti309 – 3091A → S in AAF19643. (PubMed:10942392)Curated
    Sequence conflicti347 – 3471L → R in AAF19643. (PubMed:10942392)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei162 – 18423Missing in isoform 2. 1 PublicationVSP_012304Add
    BLAST
    Alternative sequencei252 – 2598Missing in isoform 2. 1 PublicationVSP_012305

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038805 mRNA. Translation: AAH38805.1.
    BC041178 mRNA. Translation: AAH41178.1.
    AF208502 mRNA. Translation: AAF19643.1.
    CCDSiCCDS4343.1. [Q9UH73-1]
    RefSeqiNP_076870.1. NM_024007.3. [Q9UH73-1]
    NP_874367.1. NM_182708.1. [Q9UH73-2]
    UniGeneiHs.573143.

    Genome annotation databases

    EnsembliENST00000313708; ENSP00000322898; ENSG00000164330. [Q9UH73-1]
    ENST00000380654; ENSP00000370029; ENSG00000164330. [Q9UH73-2]
    GeneIDi1879.
    KEGGihsa:1879.
    UCSCiuc003lxl.5. human. [Q9UH73-2]
    uc010jip.3. human. [Q9UH73-1]

    Polymorphism databases

    DMDMi47117917.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038805 mRNA. Translation: AAH38805.1 .
    BC041178 mRNA. Translation: AAH41178.1 .
    AF208502 mRNA. Translation: AAF19643.1 .
    CCDSi CCDS4343.1. [Q9UH73-1 ]
    RefSeqi NP_076870.1. NM_024007.3. [Q9UH73-1 ]
    NP_874367.1. NM_182708.1. [Q9UH73-2 ]
    UniGenei Hs.573143.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LYR X-ray 2.51 A 10-250 [» ]
    3MQI X-ray 2.30 A/B/C 258-351 [» ]
    ProteinModelPortali Q9UH73.
    SMRi Q9UH73. Positions 35-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108211. 4 interactions.
    IntActi Q9UH73. 1 interaction.
    STRINGi 9606.ENSP00000322898.

    PTM databases

    PhosphoSitei Q9UH73.

    Polymorphism databases

    DMDMi 47117917.

    Proteomic databases

    MaxQBi Q9UH73.
    PaxDbi Q9UH73.
    PRIDEi Q9UH73.

    Protocols and materials databases

    DNASUi 1879.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000313708 ; ENSP00000322898 ; ENSG00000164330 . [Q9UH73-1 ]
    ENST00000380654 ; ENSP00000370029 ; ENSG00000164330 . [Q9UH73-2 ]
    GeneIDi 1879.
    KEGGi hsa:1879.
    UCSCi uc003lxl.5. human. [Q9UH73-2 ]
    uc010jip.3. human. [Q9UH73-1 ]

    Organism-specific databases

    CTDi 1879.
    GeneCardsi GC05M158123.
    HGNCi HGNC:3126. EBF1.
    MIMi 164343. gene.
    neXtProti NX_Q9UH73.
    PharmGKBi PA162384225.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG259050.
    HOGENOMi HOG000092311.
    HOVERGENi HBG005108.
    InParanoidi Q9UH73.
    KOi K09103.
    OMAi HAATPCI.
    OrthoDBi EOG7J446V.
    PhylomeDBi Q9UH73.
    TreeFami TF313391.

    Enzyme and pathway databases

    Reactomei REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    ChiTaRSi EBF1. human.
    EvolutionaryTracei Q9UH73.
    GeneWikii EBF1.
    GenomeRNAii 1879.
    NextBioi 7685.
    PROi Q9UH73.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UH73.
    Bgeei Q9UH73.
    CleanExi HS_EBF1.
    Genevestigatori Q9UH73.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002909. IPT.
    IPR003523. Transcription_factor_COE.
    IPR018350. Transcription_factor_COE_CS.
    [Graphical view ]
    PANTHERi PTHR10747. PTHR10747. 1 hit.
    Pfami PF01833. TIG. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    SM00429. IPT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    PROSITEi PS01345. COE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Testis.
    2. "Cloning of human early B-cell factor and identification of target genes suggest a conserved role in B-cell development in man and mouse."
      Gisler R., Jacobsen S.E., Sigvardsson M.
      Blood 96:1457-1464(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-591 (ISOFORM 1).
    3. "Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells."
      Bond H.M., Mesuraca M., Carbone E., Bonelli P., Agosti V., Amodio N., De Rosa G., Di Nicola M., Gianni A.M., Moore M.A., Hata A., Grieco M., Morrone G., Venuta S.
      Blood 103:2062-2070(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZNF521.
    4. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA-binding proteins and a novel dimerization motif."
      Siponen M.I., Wisniewska M., Lehtio L., Johansson I., Svensson L., Raszewski G., Nilsson L., Sigvardsson M., Berglund H.
      J. Biol. Chem. 285:25875-25879(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 10-351 IN COMPLEX WITH ZINC IONS.

    Entry informationi

    Entry nameiCOE1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UH73
    Secondary accession number(s): Q8IW11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3