Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CMRF35-like molecule 8

Gene

CD300A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.3 Publications

GO - Molecular functioni

  • phosphatidylethanolamine binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB
  • signaling receptor activity Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • immune system process Source: UniProtKB-KW
  • negative regulation of activation of JAK2 kinase activity Source: UniProtKB
  • negative regulation of B cell proliferation Source: UniProtKB
  • negative regulation of B cell receptor signaling pathway Source: UniProtKB
  • negative regulation of eosinophil activation Source: UniProtKB
  • negative regulation of eosinophil migration Source: UniProtKB
  • negative regulation of fibroblast proliferation Source: UniProtKB
  • negative regulation of MAP kinase activity Source: UniProtKB
  • negative regulation of mast cell activation involved in immune response Source: UniProtKB
  • negative regulation of mast cell degranulation Source: UniProtKB
  • negative regulation of neutrophil activation Source: UniProtKB
  • negative regulation of NK T cell activation Source: UniProtKB
  • negative regulation of phagocytosis, engulfment Source: UniProtKB
  • positive regulation of phosphoprotein phosphatase activity Source: UniProtKB
  • regulation of immune response Source: Reactome
  • regulation of T cell receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Cell adhesion, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
CMRF35-like molecule 8
Short name:
CLM-8
Alternative name(s):
CD300 antigen-like family member A
CMRF-35-H9
Short name:
CMRF35-H9
CMRF35-H
IRC1/IRC2
Immunoglobulin superfamily member 12
Short name:
IgSF12
Inhibitory receptor protein 60
Short name:
IRp60
NK inhibitory receptor
CD_antigen: CD300a
Gene namesi
Name:CD300A
Synonyms:CMRF35H, IGSF12
ORF Names:HSPC083
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:19319. CD300A.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 180ExtracellularSequence analysisAdd BLAST163
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 299CytoplasmicSequence analysisAdd BLAST98

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi11314.
OpenTargetsiENSG00000167851.
PharmGKBiPA142672149.

Polymorphism and mutation databases

BioMutaiCD300A.
DMDMi126302534.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001468218 – 299CMRF35-like molecule 8Add BLAST282

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 103PROSITE-ProRule annotation
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Modified residuei293PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9UGN4.
PeptideAtlasiQ9UGN4.
PRIDEiQ9UGN4.
TopDownProteomicsiQ9UGN4-1. [Q9UGN4-1]

PTM databases

iPTMnetiQ9UGN4.
PhosphoSitePlusiQ9UGN4.

Expressioni

Tissue specificityi

Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.3 Publications

Gene expression databases

BgeeiENSG00000167851.
CleanExiHS_CD300A.
ExpressionAtlasiQ9UGN4. baseline and differential.
GenevisibleiQ9UGN4. HS.

Organism-specific databases

HPAiHPA011645.

Interactioni

Subunit structurei

Upon tyrosine-phosphorylation, interacts with PTN6/SHP-1 and PTPN11/SHP-2 and INPP5D.

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT38O760153EBI-10320732,EBI-1047263

Protein-protein interaction databases

BioGridi116445. 2 interactors.
IntActiQ9UGN4. 1 interactor.
STRINGi9606.ENSP00000353259.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 27Combined sources8
Beta strandi32 – 37Combined sources6
Helixi40 – 42Combined sources3
Beta strandi45 – 51Combined sources7
Beta strandi54 – 57Combined sources4
Beta strandi61 – 66Combined sources6
Beta strandi70 – 72Combined sources3
Beta strandi75 – 80Combined sources6
Helixi81 – 83Combined sources3
Beta strandi85 – 92Combined sources8
Helixi95 – 97Combined sources3
Beta strandi99 – 106Combined sources8
Beta strandi117 – 125Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q87X-ray1.70A/B/C19-125[»]
ProteinModelPortaliQ9UGN4.
SMRiQ9UGN4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UGN4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 123Ig-like V-typeAdd BLAST105

Sequence similaritiesi

Belongs to the CD300 family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IXT8. Eukaryota.
ENOG410ZH9V. LUCA.
GeneTreeiENSGT00470000042273.
HOVERGENiHBG100050.
InParanoidiQ9UGN4.
KOiK06719.
OMAiAWRMFQK.
OrthoDBiEOG091G0RAD.
PhylomeDBiQ9UGN4.
TreeFamiTF334441.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UGN4-1) [UniParc]FASTAAdd to basket
Also known as: IRC1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWLPWALLLL WVPGCFALSK CRTVAGPVGG SLSVQCPYEK EHRTLNKYWC
60 70 80 90 100
RPPQIFLCDK IVETKGSAGK RNGRVSIRDS PANLSFTVTL ENLTEEDAGT
110 120 130 140 150
YWCGVDTPWL RDFHDPVVEV EVSVFPASTS MTPASITAAK TSTITTAFPP
160 170 180 190 200
VSSTTLFAVG ATHSASIQEE TEEVVNSQLP LLLSLLALLL LLLVGASLLA
210 220 230 240 250
WRMFQKWIKA GDHSELSQNP KQAATQSELH YANLELLMWP LQEKPAPPRE
260 270 280 290
VEVEYSTVAS PREELHYASV VFDSNTNRIA AQRPREEEPD SDYSVIRKT
Length:299
Mass (Da):33,201
Last modified:February 20, 2007 - v2
Checksum:i978461DA87E86269
GO
Isoform 2 (identifier: Q9UGN4-2) [UniParc]FASTAAdd to basket
Also known as: IRC1c

The sequence of this isoform differs from the canonical sequence as follows:
     14-126: Missing.

Show »
Length:186
Mass (Da):20,640
Checksum:i00D5E9379190EEEE
GO
Isoform 3 (identifier: Q9UGN4-3) [UniParc]FASTAAdd to basket
Also known as: IRC1b

The sequence of this isoform differs from the canonical sequence as follows:
     14-209: Missing.

Show »
Length:103
Mass (Da):11,870
Checksum:iB65239E9760E47AB
GO
Isoform 4 (identifier: Q9UGN4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-126: Missing.
     223-258: Missing.

Show »
Length:150
Mass (Da):16,517
Checksum:iED357EBD18A30FF0
GO

Sequence cautioni

The sequence AAF28906 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence AAF28906 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28 – 29VG → W in AAD01646 (PubMed:9701027).Curated2
Sequence conflicti28 – 29VG → W in AAF89957 (PubMed:10746781).Curated2
Sequence conflicti39E → Q in AAF89957 (PubMed:10746781).Curated1
Sequence conflicti189L → M in AAF28906 (Ref. 5) Curated1
Sequence conflicti193L → M in AAF28906 (Ref. 5) Curated1
Sequence conflicti209K → KWIK in AAD01646 (PubMed:9701027).Curated1
Sequence conflicti260S → F in AAF28906 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030797111R → Q.2 PublicationsCorresponds to variant rs2272111dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01055814 – 209Missing in isoform 3. 1 PublicationAdd BLAST196
Alternative sequenceiVSP_01055914 – 126Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST113
Alternative sequenceiVSP_041246223 – 258Missing in isoform 4. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238323 mRNA. Translation: CAB66145.1.
AJ010101 mRNA. Translation: CAB52291.1.
AJ010102 mRNA. Translation: CAB52292.1.
AJ010103 mRNA. Translation: CAB52293.1.
AJ224864 mRNA. Translation: CAB55347.1.
AF020314 mRNA. Translation: AAD01646.1.
AF176991
, AF176985, AF176986, AF176987, AF176988, AF176989, AF176990 Genomic DNA. Translation: AAF89957.1.
AF161346 mRNA. Translation: AAF28906.1. Sequence problems.
AC079325 Genomic DNA. No translation available.
BC032352 mRNA. Translation: AAH32352.1.
CCDSiCCDS32720.1. [Q9UGN4-1]
CCDS58590.1. [Q9UGN4-2]
CCDS82196.1. [Q9UGN4-4]
RefSeqiNP_001243770.1. NM_001256841.1. [Q9UGN4-2]
NP_001317386.1. NM_001330457.1.
NP_009192.2. NM_007261.3. [Q9UGN4-1]
UniGeneiHs.9688.

Genome annotation databases

EnsembliENST00000310828; ENSP00000308188; ENSG00000167851. [Q9UGN4-2]
ENST00000360141; ENSP00000353259; ENSG00000167851. [Q9UGN4-1]
ENST00000361933; ENSP00000354564; ENSG00000167851. [Q9UGN4-3]
ENST00000392625; ENSP00000376401; ENSG00000167851. [Q9UGN4-4]
GeneIDi11314.
KEGGihsa:11314.
UCSCiuc002jkv.5. human. [Q9UGN4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238323 mRNA. Translation: CAB66145.1.
AJ010101 mRNA. Translation: CAB52291.1.
AJ010102 mRNA. Translation: CAB52292.1.
AJ010103 mRNA. Translation: CAB52293.1.
AJ224864 mRNA. Translation: CAB55347.1.
AF020314 mRNA. Translation: AAD01646.1.
AF176991
, AF176985, AF176986, AF176987, AF176988, AF176989, AF176990 Genomic DNA. Translation: AAF89957.1.
AF161346 mRNA. Translation: AAF28906.1. Sequence problems.
AC079325 Genomic DNA. No translation available.
BC032352 mRNA. Translation: AAH32352.1.
CCDSiCCDS32720.1. [Q9UGN4-1]
CCDS58590.1. [Q9UGN4-2]
CCDS82196.1. [Q9UGN4-4]
RefSeqiNP_001243770.1. NM_001256841.1. [Q9UGN4-2]
NP_001317386.1. NM_001330457.1.
NP_009192.2. NM_007261.3. [Q9UGN4-1]
UniGeneiHs.9688.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q87X-ray1.70A/B/C19-125[»]
ProteinModelPortaliQ9UGN4.
SMRiQ9UGN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116445. 2 interactors.
IntActiQ9UGN4. 1 interactor.
STRINGi9606.ENSP00000353259.

PTM databases

iPTMnetiQ9UGN4.
PhosphoSitePlusiQ9UGN4.

Polymorphism and mutation databases

BioMutaiCD300A.
DMDMi126302534.

Proteomic databases

PaxDbiQ9UGN4.
PeptideAtlasiQ9UGN4.
PRIDEiQ9UGN4.
TopDownProteomicsiQ9UGN4-1. [Q9UGN4-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310828; ENSP00000308188; ENSG00000167851. [Q9UGN4-2]
ENST00000360141; ENSP00000353259; ENSG00000167851. [Q9UGN4-1]
ENST00000361933; ENSP00000354564; ENSG00000167851. [Q9UGN4-3]
ENST00000392625; ENSP00000376401; ENSG00000167851. [Q9UGN4-4]
GeneIDi11314.
KEGGihsa:11314.
UCSCiuc002jkv.5. human. [Q9UGN4-1]

Organism-specific databases

CTDi11314.
DisGeNETi11314.
GeneCardsiCD300A.
HGNCiHGNC:19319. CD300A.
HPAiHPA011645.
MIMi606790. gene.
neXtProtiNX_Q9UGN4.
OpenTargetsiENSG00000167851.
PharmGKBiPA142672149.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXT8. Eukaryota.
ENOG410ZH9V. LUCA.
GeneTreeiENSGT00470000042273.
HOVERGENiHBG100050.
InParanoidiQ9UGN4.
KOiK06719.
OMAiAWRMFQK.
OrthoDBiEOG091G0RAD.
PhylomeDBiQ9UGN4.
TreeFamiTF334441.

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ9UGN4.
GeneWikiiCD300A.
GenomeRNAii11314.
PROiQ9UGN4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167851.
CleanExiHS_CD300A.
ExpressionAtlasiQ9UGN4. baseline and differential.
GenevisibleiQ9UGN4. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLM8_HUMAN
AccessioniPrimary (citable) accession number: Q9UGN4
Secondary accession number(s): A8MW96
, O95100, Q9HD97, Q9P0F3, Q9UBK4, Q9UMS9, Q9UMT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.