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Q9UGM1 (ACHA9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit alpha-9
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-9
Short name=NACHR alpha-9
Gene names
Name:CHRNA9
Synonyms:NACHRA9
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion. Ref.1 Ref.5

Subunit structure

Can form homo- or hetero-oligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in cochlea, keratinocytes, pituitary gland, B-cells and T-cells. Ref.1 Ref.5 Ref.6

Miscellaneous

The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-9/CHRNA9 sub-subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 479454Neuronal acetylcholine receptor subunit alpha-9
PRO_0000000371

Regions

Topological domain26 – 237212Extracellular Potential
Transmembrane238 – 26225Helical; Potential
Transmembrane269 – 28719Helical; Potential
Transmembrane302 – 32322Helical; Potential
Topological domain324 – 457134Cytoplasmic Potential
Transmembrane458 – 47619Helical; Potential

Amino acid modifications

Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential
Disulfide bond155 ↔ 169 By similarity
Disulfide bond219 ↔ 220Associated with receptor activation By similarity

Natural variations

Natural variant961R → Q.
Corresponds to variant rs10024518 [ dbSNP | Ensembl ].
VAR_031151
Natural variant3151A → V.
Corresponds to variant rs55633891 [ dbSNP | Ensembl ].
VAR_060996
Natural variant4421N → S. Ref.1 Ref.2 Ref.4
Corresponds to variant rs10009228 [ dbSNP | Ensembl ].
VAR_025425

Sequences

Sequence LengthMass (Da)Tools
Q9UGM1 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: AA6B46B559D6111D

FASTA47954,807
        10         20         30         40         50         60 
MNWSHSCISF CWIYFAASRL RAAETADGKY AQKLFNDLFE DYSNALRPVE DTDKVLNVTL 

        70         80         90        100        110        120 
QITLSQIKDM DERNQILTAY LWIRQIWHDA YLTWDRDQYD GLDSIRIPSD LVWRPDIVLY 

       130        140        150        160        170        180 
NKADDESSEP VNTNVVLRYD GLITWDAPAI TKSSCVVDVT YFPFDNQQCN LTFGSWTYNG 

       190        200        210        220        230        240 
NQVDIFNALD SGDLSDFIED VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY 

       250        260        270        280        290        300 
IVNLLIPCVL ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG 

       310        320        330        340        350        360 
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWARVV ILKYMSRVLF VYDVGESCLS 

       370        380        390        400        410        420 
PHHSRERDHL TKVYSKLPES NLKAARNKDL SRKKDMNKRL KNDLGCQGKN PQEAESYCAQ 

       430        440        450        460        470 
YKVLTRNIEY IAKCLKDHKA TNSKGSEWKK VAKVIDRFFM WIFFIMVFVM TILIIARAD 

« Hide

References

« Hide 'large scale' references
[1]"A novel human nicotinic receptor subunit, alpha10, that confers functionality to the alpha9-subunit."
Sgard F., Charpantier E., Bertrand S., Walker N., Caput D., Graham D., Bertrand D., Besnard F.
Mol. Pharmacol. 61:150-159(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CHRNA10, TISSUE SPECIFICITY, VARIANT SER-442.
Tissue: Embryo.
[2]"Chromosome location and characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) gene."
Lustig L.R., Peng H.
Cytogenet. Genome Res. 98:154-159(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-442.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-442.
[5]"Novel human alpha9 acetylcholine receptor regulating keratinocyte adhesion is targeted by Pemphigus vulgaris autoimmunity."
Nguyen V.T., Ndoye A., Grando S.A.
Am. J. Pathol. 157:1377-1391(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-476, FUNCTION, TISSUE SPECIFICITY.
[6]"Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes."
Peng H., Ferris R.L., Matthews T., Hiel H., Lopez-Albaitero A., Lustig L.R.
Life Sci. 76:263-280(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243342 mRNA. Translation: CAB65091.1.
AY123244 Genomic DNA. Translation: AAM74523.1.
AC118275 Genomic DNA. Translation: AAY40986.1.
BC113549 mRNA. Translation: AAI13550.1.
BC113575 mRNA. Translation: AAI13576.1.
AF227732 mRNA. Translation: AAF61920.1.
RefSeqNP_060051.2. NM_017581.3.
UniGeneHs.272278.

3D structure databases

ProteinModelPortalQ9UGM1.
SMRQ9UGM1. Positions 28-478.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120731. 2 interactions.
STRING9606.ENSP00000312663.

Chemistry

BindingDBQ9UGM1.
ChEMBLCHEMBL2184.
DrugBankDB00184. Nicotine.
GuidetoPHARMACOLOGY469.

PTM databases

PhosphoSiteQ9UGM1.

Polymorphism databases

DMDM308153406.

Proteomic databases

PaxDbQ9UGM1.
PRIDEQ9UGM1.

Protocols and materials databases

DNASU55584.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310169; ENSP00000312663; ENSG00000174343.
GeneID55584.
KEGGhsa:55584.
UCSCuc003gva.2. human.

Organism-specific databases

CTD55584.
GeneCardsGC04P040337.
H-InvDBHIX0031380.
HGNCHGNC:14079. CHRNA9.
MIM605116. gene.
neXtProtNX_Q9UGM1.
PharmGKBPA26493.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG306809.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidQ9UGM1.
KOK04810.
OMASYCAQYK.
OrthoDBEOG72JWGV.
PhylomeDBQ9UGM1.
TreeFamTF315605.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

BgeeQ9UGM1.
CleanExHS_CHRNA9.
GenevestigatorQ9UGM1.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCHRNA9.
GenomeRNAi55584.
NextBio60096.
PROQ9UGM1.
SOURCESearch...

Entry information

Entry nameACHA9_HUMAN
AccessionPrimary (citable) accession number: Q9UGM1
Secondary accession number(s): Q14CY7, Q4W5A2, Q9NYV2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 5, 2010
Last modified: March 19, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM