Q9UGK3 (STAP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-transducing adaptor protein 2
Short name=STAP-2

Alternative name(s):
Breast tumor kinase substrate
Short name=BRK substrate

Gene names

Name:	STAP2
Synonyms:	BKS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate of protein kinase PTK6. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity. Ref.1
Subunit structure	Interacts with PTK6 and CSF1R. Ref.1 Ref.6
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Widely expressed. Ref.1 Ref.5
Post-translational modification	Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC. Ref.5 Ref.6
Sequence similarities	Contains 1 PH domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil SH2 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IKBKB	O14920	7	EBI-1553984,EBI-81266
MYD88	Q99836	3	EBI-1553984,EBI-447677

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UGK3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UGK3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     356-356: K → KPVEKGFHHVAQAGLELLTSSDPPTSASQSAGITGVSHHTWPHLSSL

Note: Alu insert from position 358 to 403.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

Signal-transducing adaptor protein 2

PRO_0000072239

Regions

Domain

18 – 130

113

PH

Domain

133 – 248

116

SH2

Coiled coil

382 – 402

21

Potential

Compositional bias

271 – 359

89

Pro-rich

Amino acid modifications

Modified residue

22

1

Phosphotyrosine; by SRC Probable

Modified residue

250

1

Phosphotyrosine; by PTK6 Ref.5 Ref.6

Modified residue

310

1

Phosphotyrosine Probable

Modified residue

322

1

Phosphotyrosine; by SRC Probable

Natural variations

Alternative sequence

356

1

K → KPVEKGFHHVAQAGLELLTS SDPPTSASQSAGITGVSHHT WPHLSSL in isoform 2.

VSP_041403

Natural variant

93

1

D → N. Ref.1 Ref.2 Ref.4
Corresponds to variant rs7247504 [ dbSNP | Ensembl ].

VAR_055239

Experimental info

Mutagenesis

22

1

Y → F: Small decrease in tyrosine phosphorylation. Ref.5

Mutagenesis

250

1

Y → F: Loss of tyrosine phosphorylation. Ref.5

Mutagenesis

310

1

Y → F: Decrease in tyrosine phosphorylation. Ref.5

Mutagenesis

322

1

Y → F: Decrease in tyrosine phosphorylation. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

403

Helix Strand Turn

Details...

Beta strand

148 – 150

3

Helix

159 – 168

10

Beta strand

174 – 179

6

Beta strand

182 – 191

10

Beta strand

202 – 204

3

Beta strand

215 – 219

5

Helix

226 – 236

11

Beta strand

237 – 239

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: A5E809B3F233EFD0	FASTA	403	44,894
	10 20 30 40 50 60 MASALRPPRV PKPKGVLPSH YYESFLEKKG PCDRDYKKFW AGLQGLTIYF YNSNRDFQHV 70 80 90 100 110 120 EKLNLGAFEK LTDEIPWGSS RDPGTHFSLI LRDQEIKFKV ETLECREMWK GFILTVVELR 130 140 150 160 170 180 VPTDLTLLPG HLYMMSEVLA KEEARRALET PSCFLKVSRL EAQLLLERYP ECGNLLLRPS 190 200 210 220 230 240 GDGADGVSVT TRQMHNGTHV VRHYKVKREG PKYVIDVEQP FSCTSLDAVV NYFVSHTKKA 250 260 270 280 290 300 LVPFLLDEDY EKVLGYVEAD KENGENVWVA PSAPGPGPAP CTGGPKPLSP ASSQDKLPPL 310 320 330 340 350 360 PPLPNQEENY VTPIGDGPAV DYENQDVASS SWPVILKPKK LPKPPAKLPK PPVGPKPEPK 370 380 390 400 VFNGGLGRKL PVSSAQPLFP TAGLADMTAE LQKKLEKRRA LEH
	Isoform 2 [UniParc]. Checksum: 31A9048F7B0E750F	FASTA	449	49,649
	10 20 30 40 50 60 MASALRPPRV PKPKGVLPSH YYESFLEKKG PCDRDYKKFW AGLQGLTIYF YNSNRDFQHV 70 80 90 100 110 120 EKLNLGAFEK LTDEIPWGSS RDPGTHFSLI LRDQEIKFKV ETLECREMWK GFILTVVELR 130 140 150 160 170 180 VPTDLTLLPG HLYMMSEVLA KEEARRALET PSCFLKVSRL EAQLLLERYP ECGNLLLRPS 190 200 210 220 230 240 GDGADGVSVT TRQMHNGTHV VRHYKVKREG PKYVIDVEQP FSCTSLDAVV NYFVSHTKKA 250 260 270 280 290 300 LVPFLLDEDY EKVLGYVEAD KENGENVWVA PSAPGPGPAP CTGGPKPLSP ASSQDKLPPL 310 320 330 340 350 360 PPLPNQEENY VTPIGDGPAV DYENQDVASS SWPVILKPKK LPKPPAKLPK PPVGPKPVEK 370 380 390 400 410 420 GFHHVAQAGL ELLTSSDPPT SASQSAGITG VSHHTWPHLS SLPEPKVFNG GLGRKLPVSS 430 440 AQPLFPTAGL ADMTAELQKK LEKRRALEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK." Mitchell P.J., Sara E.A., Crompton M.R. Oncogene 19:4273-4282(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH PTK6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT ASN-93. Tissue: Mammary gland.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ASN-93. Tissue: Colon mucosa.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASN-93. Tissue: Placenta.
[5]	"STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif." Minoguchi M., Minoguchi S., Aki D., Joo A., Yamamoto T., Yumioka T., Matsuda T., Yoshimura A. J. Biol. Chem. 278:11182-11189(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-22; TYR-250; TYR-310 AND TYR-322, MUTAGENESIS OF TYR-22; TYR-250; TYR-310 AND TYR-322.
[6]	"STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation." Ikeda O., Miyasaka Y., Sekine Y., Mizushima A., Muromoto R., Nanbo A., Yoshimura A., Matsuda T. Biochem. Biophys. Res. Commun. 384:71-75(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT TYR-250 BY PTK6, INTERACTION WITH PTK6.
[7]	"Solution structure of the human STAP2 SH2 domain." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 137-247.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ245719 mRNA. Translation: CAB65105.1.
AK000241 mRNA. Translation: BAA91028.1.
AC008616 Genomic DNA. No translation available.
BC000795 mRNA. Translation: AAH00795.1.

IPI

IPI00015096.
IPI00383883.

RefSeq

NP_001013863.1. NM_001013841.1.
NP_060190.2. NM_017720.2.

UniGene

Hs.194385.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EL8	NMR	-	A	137-247	[»]

ProteinModelPortal

Q9UGK3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UGK3. 4 interactions.

MINT

MINT-1213508.

STRING

9606.ENSP00000317912.

PTM databases

PhosphoSite

Q9UGK3.

Polymorphism databases

DMDM

229462752.

Proteomic databases

PaxDb

Q9UGK3.

PRIDE

Q9UGK3.

Protocols and materials databases

DNASU

55620.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000314714; ENSP00000317912; ENSG00000178078.
ENST00000424810; ENSP00000411838; ENSG00000178078.
ENST00000594605; ENSP00000471052; ENSG00000178078.
ENST00000600324; ENSP00000468927; ENSG00000178078.

GeneID

55620.

KEGG

hsa:55620.

UCSC

uc002mab.3. human.
uc002mac.3. human.

Organism-specific databases

CTD

55620.

GeneCards

GC19M004324.

H-InvDB

HIX0018702.

HGNC

HGNC:30430. STAP2.

HPA

HPA002375.
HPA027761.

MIM

607881. gene.

neXtProt

NX_Q9UGK3.

PharmGKB

PA162404971.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG43046.

HOGENOM

HOG000063725.

HOVERGEN

HBG108516.

OMA

LYMMAEA.

OrthoDB

EOG4X3H1R.

Gene expression databases

Bgee

Q9UGK3.

CleanEx

HS_STAP2.

Genevestigator

Q9UGK3.

Family and domain databases

Gene3D

3.30.505.10. 1 hit.

InterPro

IPR001849. Pleckstrin_homology.
IPR000980. SH2.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. False negative.
PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9UGK3.

GenomeRNAi

55620.

NextBio

60220.

SOURCE

Search...

Entry information

Entry name

STAP2_HUMAN

Accession

Primary (citable) accession number: Q9UGK3
Secondary accession number(s): A6NKK3, Q9NXI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.