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Protein

Gamma-tubulin complex component 4

Gene

TUBGCP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

GO - Molecular functioni

  • gamma-tubulin binding Source: GO_Central
  • structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-380320. Recruitment of NuMA to mitotic centrosomes.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-tubulin complex component 4
Short name:
GCP-4
Short name:
hGCP4
Alternative name(s):
Gamma-ring complex protein 76 kDa
Short name:
h76p
Short name:
hGrip76
Gene namesi
Name:TUBGCP4
Synonyms:76P, GCP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:16691. TUBGCP4.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytosol Source: Reactome
  • equatorial microtubule organizing center Source: GO_Central
  • gamma-tubulin complex Source: GO_Central
  • gamma-tubulin ring complex Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: ProtInc
  • recycling endosome Source: UniProtKB
  • spindle pole body Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

Microcephaly and chorioretinopathy, autosomal recessive, 3 (MCCRP3)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by congenital microcephaly and chorioretinal dysplasia associated with poor vision and nystagmus. Variable ocular anomalies include microphthalmia, retinal folding, retinal detachment, optic nerve hypoplasia, absence of retinal vessels, round areas of chorioretinal atrophy, and attenuated electroretinogram. Most patients have mild developmental delay and mild learning difficulties.
See also OMIM:616335

Keywords - Diseasei

Mental retardation, Primary microcephaly

Organism-specific databases

MIMi616335. phenotype.
PharmGKBiPA162407404.

Polymorphism and mutation databases

BioMutaiTUBGCP4.
DMDMi22095730.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 667667Gamma-tubulin complex component 4PRO_0000078124Add
BLAST

Proteomic databases

EPDiQ9UGJ1.
MaxQBiQ9UGJ1.
PaxDbiQ9UGJ1.
PeptideAtlasiQ9UGJ1.
PRIDEiQ9UGJ1.

PTM databases

iPTMnetiQ9UGJ1.
PhosphoSiteiQ9UGJ1.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSG00000137822.
CleanExiHS_TUBGCP4.
ExpressionAtlasiQ9UGJ1. baseline and differential.
GenevisibleiQ9UGJ1. HS.

Organism-specific databases

HPAiHPA043212.

Interactioni

Subunit structurei

Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with NINL. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome (PubMed:26213385).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GLYCTKQ8IVS83EBI-1052544,EBI-748515
RNF146Q9NTX73EBI-1052544,EBI-722397

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118078. 101 interactions.
DIPiDIP-50536N.
IntActiQ9UGJ1. 36 interactions.
MINTiMINT-1440401.
STRINGi9606.ENSP00000456648.

Structurei

Secondary structure

1
667
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 98Combined sources
Beta strandi15 – 206Combined sources
Turni21 – 233Combined sources
Beta strandi24 – 263Combined sources
Helixi36 – 6126Combined sources
Helixi84 – 9613Combined sources
Helixi98 – 11316Combined sources
Helixi119 – 1257Combined sources
Helixi127 – 14620Combined sources
Helixi151 – 16212Combined sources
Helixi166 – 19328Combined sources
Beta strandi203 – 2075Combined sources
Beta strandi257 – 2593Combined sources
Helixi261 – 2633Combined sources
Helixi270 – 28415Combined sources
Helixi303 – 31513Combined sources
Helixi322 – 34726Combined sources
Helixi350 – 36011Combined sources
Helixi366 – 38015Combined sources
Helixi388 – 40215Combined sources
Helixi411 – 4133Combined sources
Beta strandi414 – 4185Combined sources
Turni449 – 4524Combined sources
Beta strandi453 – 4575Combined sources
Helixi463 – 4653Combined sources
Helixi469 – 49931Combined sources
Helixi502 – 5043Combined sources
Helixi509 – 53527Combined sources
Helixi537 – 54913Combined sources
Helixi554 – 57421Combined sources
Helixi576 – 59722Combined sources
Helixi606 – 63530Combined sources
Helixi640 – 65415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RIPX-ray2.30A1-667[»]
ProteinModelPortaliQ9UGJ1.
SMRiQ9UGJ1. Positions 1-655.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UGJ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the TUBGCP family.Curated

Phylogenomic databases

eggNOGiKOG2065. Eukaryota.
ENOG410XPES. LUCA.
GeneTreeiENSGT00840000129807.
HOGENOMiHOG000234444.
HOVERGENiHBG023386.
InParanoidiQ9UGJ1.
KOiK16571.
OMAiQHTGHVH.
OrthoDBiEOG091G01V2.
PhylomeDBiQ9UGJ1.
TreeFamiTF324188.

Family and domain databases

InterProiIPR007259. GCP.
[Graphical view]
PANTHERiPTHR19302. PTHR19302. 1 hit.
PfamiPF04130. Spc97_Spc98. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UGJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHELLLALS GYPGSIFTWN KRSGLQVSQD FPFLHPSETS VLNRLCRLGT
60 70 80 90 100
DYIRFTEFIE QYTGHVQQQD HHPSQQGQGG LHGIYLRAFC TGLDSVLQPY
110 120 130 140 150
RQALLDLEQE FLGDPHLSIS HVNYFLDQFQ LLFPSVMVVV EQIKSQKIHG
160 170 180 190 200
CQILETVYKH SCGGLPPVRS ALEKILAVCH GVMYKQLSAW MLHGLLLDQH
210 220 230 240 250
EEFFIKQGPS SGNVSAQPEE DEEDLGIGGL TGKQLRELQD LRLIEEENML
260 270 280 290 300
APSLKQFSLR VEILPSYIPV RVAEKILFVG ESVQMFENQN VNLTRKGSIL
310 320 330 340 350
KNQEDTFAAE LHRLKQQPLF SLVDFEQVVD RIRSTVAEHL WKLMVEESDL
360 370 380 390 400
LGQLKIIKDF YLLGRGELFQ AFIDTAQHML KTPPTAVTEH DVNVAFQQSA
410 420 430 440 450
HKVLLDDDNL LPLLHLTIEY HGKEHKADAT QAREGPSRET SPREAPASGW
460 470 480 490 500
AALGLSYKVQ WPLHILFTPA VLEKYNVVFK YLLSVRRVQA ELQHCWALQM
510 520 530 540 550
QRKHLKSNQT DAIKWRLRNH MAFLVDNLQY YLQVDVLESQ FSQLLHQINS
560 570 580 590 600
TRDFESIRLA HDHFLSNLLA QSFILLKPVF HCLNEILDLC HSFCSLVSQN
610 620 630 640 650
LGPLDERGAA QLSILVKGFS RQSSLLFKIL SSVRNHQINS DLAQLLLRLD
660
YNKYYTQAGG TLGSFGM
Length:667
Mass (Da):76,089
Last modified:May 1, 2000 - v1
Checksum:i2030EEDF290F5FAC
GO
Isoform 2 (identifier: Q9UGJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     427-427: Missing.

Show »
Length:666
Mass (Da):76,018
Checksum:i5BF032C026829F3A
GO

Sequence cautioni

The sequence BAA91802 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei427 – 4271Missing in isoform 2. 1 PublicationVSP_040085

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249677 mRNA. Translation: CAB62539.1.
AK001639 mRNA. Translation: BAA91802.1. Different initiation.
AK027703 mRNA. Translation: BAG51368.1.
AC018924 Genomic DNA. No translation available.
CH471125 Genomic DNA. Translation: EAW92603.1.
BC009870 mRNA. Translation: AAH09870.1.
BC012801 mRNA. Translation: AAH12801.1.
CCDSiCCDS42030.1. [Q9UGJ1-2]
CCDS66745.1. [Q9UGJ1-1]
RefSeqiNP_001273343.1. NM_001286414.2. [Q9UGJ1-1]
NP_055259.2. NM_014444.4. [Q9UGJ1-2]
XP_011519757.1. XM_011521455.2.
UniGeneiHs.584887.
Hs.598799.

Genome annotation databases

EnsembliENST00000260383; ENSP00000260383; ENSG00000137822. [Q9UGJ1-1]
ENST00000564079; ENSP00000456648; ENSG00000137822. [Q9UGJ1-2]
GeneIDi27229.
KEGGihsa:27229.
UCSCiuc001zrn.5. human. [Q9UGJ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249677 mRNA. Translation: CAB62539.1.
AK001639 mRNA. Translation: BAA91802.1. Different initiation.
AK027703 mRNA. Translation: BAG51368.1.
AC018924 Genomic DNA. No translation available.
CH471125 Genomic DNA. Translation: EAW92603.1.
BC009870 mRNA. Translation: AAH09870.1.
BC012801 mRNA. Translation: AAH12801.1.
CCDSiCCDS42030.1. [Q9UGJ1-2]
CCDS66745.1. [Q9UGJ1-1]
RefSeqiNP_001273343.1. NM_001286414.2. [Q9UGJ1-1]
NP_055259.2. NM_014444.4. [Q9UGJ1-2]
XP_011519757.1. XM_011521455.2.
UniGeneiHs.584887.
Hs.598799.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RIPX-ray2.30A1-667[»]
ProteinModelPortaliQ9UGJ1.
SMRiQ9UGJ1. Positions 1-655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118078. 101 interactions.
DIPiDIP-50536N.
IntActiQ9UGJ1. 36 interactions.
MINTiMINT-1440401.
STRINGi9606.ENSP00000456648.

PTM databases

iPTMnetiQ9UGJ1.
PhosphoSiteiQ9UGJ1.

Polymorphism and mutation databases

BioMutaiTUBGCP4.
DMDMi22095730.

Proteomic databases

EPDiQ9UGJ1.
MaxQBiQ9UGJ1.
PaxDbiQ9UGJ1.
PeptideAtlasiQ9UGJ1.
PRIDEiQ9UGJ1.

Protocols and materials databases

DNASUi27229.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260383; ENSP00000260383; ENSG00000137822. [Q9UGJ1-1]
ENST00000564079; ENSP00000456648; ENSG00000137822. [Q9UGJ1-2]
GeneIDi27229.
KEGGihsa:27229.
UCSCiuc001zrn.5. human. [Q9UGJ1-1]

Organism-specific databases

CTDi27229.
GeneCardsiTUBGCP4.
H-InvDBHIX0012185.
HIX0172821.
HGNCiHGNC:16691. TUBGCP4.
HPAiHPA043212.
MIMi609610. gene.
616335. phenotype.
neXtProtiNX_Q9UGJ1.
PharmGKBiPA162407404.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2065. Eukaryota.
ENOG410XPES. LUCA.
GeneTreeiENSGT00840000129807.
HOGENOMiHOG000234444.
HOVERGENiHBG023386.
InParanoidiQ9UGJ1.
KOiK16571.
OMAiQHTGHVH.
OrthoDBiEOG091G01V2.
PhylomeDBiQ9UGJ1.
TreeFamiTF324188.

Enzyme and pathway databases

ReactomeiR-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-380320. Recruitment of NuMA to mitotic centrosomes.

Miscellaneous databases

ChiTaRSiTUBGCP4. human.
EvolutionaryTraceiQ9UGJ1.
GeneWikiiTUBGCP4.
GenomeRNAii27229.
PROiQ9UGJ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137822.
CleanExiHS_TUBGCP4.
ExpressionAtlasiQ9UGJ1. baseline and differential.
GenevisibleiQ9UGJ1. HS.

Family and domain databases

InterProiIPR007259. GCP.
[Graphical view]
PANTHERiPTHR19302. PTHR19302. 1 hit.
PfamiPF04130. Spc97_Spc98. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCP4_HUMAN
AccessioniPrimary (citable) accession number: Q9UGJ1
Secondary accession number(s): B3KNK6, Q969X3, Q9NVF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.