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Protein

Testin

Gene

TES

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth.3 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • negative regulation of cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135269-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Testin
Alternative name(s):
TESS
Gene namesi
Name:TES
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:14620. TES.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cell junction Source: HPA
  • cytoplasm Source: HPA
  • focal adhesion Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi328C → A: Abolishes interaction with ACTL7A. 1 Publication1
Mutagenesisi391C → A: Abolishes localization at focal adhesions. 1 Publication1

Organism-specific databases

DisGeNETi26136.
OpenTargetsiENSG00000135269.
PharmGKBiPA37906.

Polymorphism and mutation databases

BioMutaiTES.
DMDMi17380320.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759061 – 421TestinAdd BLAST421

Proteomic databases

PaxDbiQ9UGI8.
PeptideAtlasiQ9UGI8.
PRIDEiQ9UGI8.

PTM databases

iPTMnetiQ9UGI8.
PhosphoSitePlusiQ9UGI8.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000135269.
CleanExiHS_TES.
ExpressionAtlasiQ9UGI8. baseline and differential.
GenevisibleiQ9UGI8. HS.

Organism-specific databases

HPAiCAB003690.
HPA015269.
HPA018123.

Interactioni

Subunit structurei

Interacts via LIM domain 1 with ZYX. Interacts (via LIM domain 3) with ENAH and VASP. Interacts with ALKBH4, talin, actin, alpha-actinin, GRIP1 and PXN. Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTL7AQ9Y6159EBI-2561654,EBI-10825302
ENAHQ8N8S72EBI-2561654,EBI-2834410

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi117572. 55 interactors.
IntActiQ9UGI8. 34 interactors.
MINTiMINT-2821159.
STRINGi9606.ENSP00000350937.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni302 – 304Combined sources3
Beta strandi305 – 307Combined sources3
Beta strandi313 – 316Combined sources4
Beta strandi319 – 321Combined sources3
Helixi323 – 325Combined sources3
Turni329 – 331Combined sources3
Beta strandi338 – 343Combined sources6
Beta strandi346 – 349Combined sources4
Helixi350 – 356Combined sources7
Turni362 – 364Combined sources3
Beta strandi365 – 368Combined sources4
Beta strandi374 – 377Combined sources4
Beta strandi380 – 383Combined sources4
Turni384 – 387Combined sources4
Turni392 – 394Combined sources3
Beta strandi404 – 406Combined sources3
Beta strandi409 – 413Combined sources5
Helixi414 – 418Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IYBX-ray2.35E/F/G/H357-421[»]
2XQNX-ray2.62T296-421[»]
ProteinModelPortaliQ9UGI8.
SMRiQ9UGI8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UGI8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 199PETPROSITE-ProRule annotationAdd BLAST108
Domaini234 – 297LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST64
Domaini299 – 359LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST61
Domaini362 – 421LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 46Cys-richAdd BLAST25

Domaini

The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX.1 Publication

Sequence similaritiesi

Belongs to the prickle / espinas / testin family.Curated
Contains 3 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 PET domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiENOG410INGJ. Eukaryota.
ENOG410XQU0. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000231628.
HOVERGENiHBG001038.
InParanoidiQ9UGI8.
OMAiNNFNWHA.
OrthoDBiEOG091G07C5.
PhylomeDBiQ9UGI8.
TreeFamiTF313265.

Family and domain databases

CDDicd09829. PET_testin. 1 hit.
Gene3Di2.10.110.10. 3 hits.
InterProiIPR010442. PET_domain.
IPR033724. PET_testin.
IPR027683. Testin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24211:SF1. PTHR24211:SF1. 2 hits.
PfamiPF00412. LIM. 3 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UGI8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE
60 70 80 90 100
HDVLLSNEED RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA
110 120 130 140 150
KKNVSINTVT YEWAPPVQNQ ALARQYMQML PKEKQPVAGS EGAQYRKKQL
160 170 180 190 200
AKQLPAHDQD PSKCHELSPR EVKEMEQFVK KYKSEALGVG DVKLPCEMDA
210 220 230 240 250
QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK LSMKEGDPAI
260 270 280 290 300
YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR
310 320 330 340 350
CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK
360 370 380 390 400
PCYVKNHAVV CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG
410 420
QKFMPVEGMV FCSVECKKRM S
Length:421
Mass (Da):47,996
Last modified:May 1, 2000 - v1
Checksum:iAB9FF6669C50D492
GO
Isoform 2 (identifier: Q9UGI8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:412
Mass (Da):46,910
Checksum:iC97A6D97364B3569
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132K → E in BAB13846 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050170221A → V.Corresponds to variant rs2272193dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0031221 – 9Missing in isoform 2. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260225 Genomic DNA. Translation: AAG17635.1.
AF260225 Genomic DNA. Translation: AAG17636.1.
AF245356 mRNA. Translation: AAG17612.1.
AF245357 mRNA. Translation: AAG17613.1.
AJ250865 mRNA. Translation: CAB65119.1.
AK021575 mRNA. Translation: BAB13846.1.
AK291802 mRNA. Translation: BAF84491.1.
AC073130 Genomic DNA. Translation: AAQ93367.1.
CH236947 Genomic DNA. Translation: EAL24365.1.
BC001451 mRNA. Translation: AAH01451.1.
CCDSiCCDS5763.1. [Q9UGI8-1]
CCDS5764.1. [Q9UGI8-2]
RefSeqiNP_056456.1. NM_015641.3. [Q9UGI8-1]
NP_690042.1. NM_152829.2. [Q9UGI8-2]
UniGeneiHs.592286.
Hs.664957.

Genome annotation databases

EnsembliENST00000358204; ENSP00000350937; ENSG00000135269. [Q9UGI8-1]
ENST00000393481; ENSP00000377121; ENSG00000135269. [Q9UGI8-2]
GeneIDi26136.
KEGGihsa:26136.
UCSCiuc003vho.3. human. [Q9UGI8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260225 Genomic DNA. Translation: AAG17635.1.
AF260225 Genomic DNA. Translation: AAG17636.1.
AF245356 mRNA. Translation: AAG17612.1.
AF245357 mRNA. Translation: AAG17613.1.
AJ250865 mRNA. Translation: CAB65119.1.
AK021575 mRNA. Translation: BAB13846.1.
AK291802 mRNA. Translation: BAF84491.1.
AC073130 Genomic DNA. Translation: AAQ93367.1.
CH236947 Genomic DNA. Translation: EAL24365.1.
BC001451 mRNA. Translation: AAH01451.1.
CCDSiCCDS5763.1. [Q9UGI8-1]
CCDS5764.1. [Q9UGI8-2]
RefSeqiNP_056456.1. NM_015641.3. [Q9UGI8-1]
NP_690042.1. NM_152829.2. [Q9UGI8-2]
UniGeneiHs.592286.
Hs.664957.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IYBX-ray2.35E/F/G/H357-421[»]
2XQNX-ray2.62T296-421[»]
ProteinModelPortaliQ9UGI8.
SMRiQ9UGI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117572. 55 interactors.
IntActiQ9UGI8. 34 interactors.
MINTiMINT-2821159.
STRINGi9606.ENSP00000350937.

PTM databases

iPTMnetiQ9UGI8.
PhosphoSitePlusiQ9UGI8.

Polymorphism and mutation databases

BioMutaiTES.
DMDMi17380320.

Proteomic databases

PaxDbiQ9UGI8.
PeptideAtlasiQ9UGI8.
PRIDEiQ9UGI8.

Protocols and materials databases

DNASUi26136.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358204; ENSP00000350937; ENSG00000135269. [Q9UGI8-1]
ENST00000393481; ENSP00000377121; ENSG00000135269. [Q9UGI8-2]
GeneIDi26136.
KEGGihsa:26136.
UCSCiuc003vho.3. human. [Q9UGI8-1]

Organism-specific databases

CTDi26136.
DisGeNETi26136.
GeneCardsiTES.
HGNCiHGNC:14620. TES.
HPAiCAB003690.
HPA015269.
HPA018123.
MIMi606085. gene.
neXtProtiNX_Q9UGI8.
OpenTargetsiENSG00000135269.
PharmGKBiPA37906.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INGJ. Eukaryota.
ENOG410XQU0. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000231628.
HOVERGENiHBG001038.
InParanoidiQ9UGI8.
OMAiNNFNWHA.
OrthoDBiEOG091G07C5.
PhylomeDBiQ9UGI8.
TreeFamiTF313265.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135269-MONOMER.

Miscellaneous databases

ChiTaRSiTES. human.
EvolutionaryTraceiQ9UGI8.
GeneWikiiTestin.
GenomeRNAii26136.
PROiQ9UGI8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135269.
CleanExiHS_TES.
ExpressionAtlasiQ9UGI8. baseline and differential.
GenevisibleiQ9UGI8. HS.

Family and domain databases

CDDicd09829. PET_testin. 1 hit.
Gene3Di2.10.110.10. 3 hits.
InterProiIPR010442. PET_domain.
IPR033724. PET_testin.
IPR027683. Testin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24211:SF1. PTHR24211:SF1. 2 hits.
PfamiPF00412. LIM. 3 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTES_HUMAN
AccessioniPrimary (citable) accession number: Q9UGI8
Secondary accession number(s): A4D0U6, Q9GZQ1, Q9HAJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.