Q9UGI8 (TES_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testin

Alternative name(s):
TESS

Gene names

Name:

TES

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. Ref.2 Ref.7 Ref.8
Subunit structure	Interacts via LIM domain 1 with ZYX. Interacts (via LIM domain 3) with ENAH and VASP. Interacts with talin, actin, alpha-actinin, GRIP1 and PXN. Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer. Ref.7 Ref.8 Ref.11
Subcellular location	Cytoplasm. Cell junction › focal adhesion. Note: Detected along actin stress fibers. Ref.7 Ref.8
Tissue specificity	Ubiquitous. Ref.2
Domain	The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX. Ref.7
Sequence similarities	Belongs to the prickle / espinas / testin family. Contains 3 LIM zinc-binding domains. Contains 1 PET domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of cell proliferation Inferred from mutant phenotype Ref.2. Source: UniProtKB
Cellular_component	cell junction Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay. Source: HPA focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: HPA protein complex Inferred from direct assay Ref.11. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from direct assay Ref.11. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UGI8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UGI8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 421

421

Testin

PRO_0000075906

Regions

Domain

92 – 199

108

PET

Domain

234 – 297

64

LIM zinc-binding 1

Domain

299 – 359

61

LIM zinc-binding 2

Domain

362 – 421

60

LIM zinc-binding 3

Compositional bias

22 – 46

25

Cys-rich

Natural variations

Alternative sequence

1 – 9

9

Missing in isoform 2.

VSP_003122

Natural variant

221

1

A → V.
Corresponds to variant rs2272193 [ dbSNP | Ensembl ].

VAR_050170

Experimental info

Mutagenesis

328

1

C → A: Abolishes interaction with ACTL7A. Ref.11

Mutagenesis

391

1

C → A: Abolishes localization at focal adhesions. Ref.7

Sequence conflict

132

1

K → E in BAB13846. Ref.3

Secondary structure

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421

Helix Strand Turn

Details...

Turn

302 – 304

3

Beta strand

305 – 307

3

Beta strand

313 – 316

4

Beta strand

319 – 321

3

Helix

323 – 325

3

Turn

329 – 331

3

Beta strand

338 – 343

6

Beta strand

346 – 349

4

Helix

350 – 356

7

Turn

362 – 364

3

Beta strand

365 – 368

4

Beta strand

374 – 377

4

Beta strand

380 – 383

4

Turn

384 – 387

4

Turn

392 – 394

3

Beta strand

404 – 406

3

Beta strand

409 – 413

5

Helix

414 – 418

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: AB9FF6669C50D492	FASTA	421	47,996
	10 20 30 40 50 60 MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE HDVLLSNEED 70 80 90 100 110 120 RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA KKNVSINTVT YEWAPPVQNQ 130 140 150 160 170 180 ALARQYMQML PKEKQPVAGS EGAQYRKKQL AKQLPAHDQD PSKCHELSPR EVKEMEQFVK 190 200 210 220 230 240 KYKSEALGVG DVKLPCEMDA QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK 250 260 270 280 290 300 LSMKEGDPAI YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR 310 320 330 340 350 360 CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK PCYVKNHAVV 370 380 390 400 410 420 CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG QKFMPVEGMV FCSVECKKRM S
	Isoform 2 [UniParc]. Checksum: C97A6D97364B3569	FASTA	412	46,910
	10 20 30 40 50 60 MGLGHEQGFG APCLKCKEKC EGFELHFWRK ICRNCKCGQE EHDVLLSNEE DRKVGKLFED 70 80 90 100 110 120 TKYTTLIAKL KSDGIPMYKR NVMILTNPVA AKKNVSINTV TYEWAPPVQN QALARQYMQM 130 140 150 160 170 180 LPKEKQPVAG SEGAQYRKKQ LAKQLPAHDQ DPSKCHELSP REVKEMEQFV KKYKSEALGV 190 200 210 220 230 240 GDVKLPCEMD AQGPKQMNIP GGDRSTPAAV GAMEDKSAEH KRTQYSCYCC KLSMKEGDPA 250 260 270 280 290 300 IYAERAGYDK LWHPACFVCS TCHELLVDMI YFWKNEKLYC GRHYCDSEKP RCAGCDELIF 310 320 330 340 350 360 SNEYTQAENQ NWHLKHFCCF DCDSILAGEI YVMVNDKPVC KPCYVKNHAV VCQGCHNAID 370 380 390 400 410 PEVQRVTYNN FSWHASTECF LCSCCSKCLI GQKFMPVEGM VFCSVECKKR MS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human TESTIN gene localized in the FRA7G region at 7q31.2." Tatarelli C., Linnenbach A., Mimori K., Croce C.M. Genomics 68:1-12(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
[2]	"The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein." Tobias E.S., Hurlstone A.F.L., MacKenzie E., McFarlane R., Black D.M. Oncogene 20:2844-2853(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Embryo and Prostate.
[4]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[7]	"The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions." Garvalov B.K., Higgins T.E., Sutherland J.D., Zettl M., Scaplehorn N., Koecher T., Piddini E., Griffiths G., Way M. J. Cell Biol. 161:33-39(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF CYS-391, INTERACTION WITH ENAH; ZYX; VASP; ACTIN FIBERS; ALPHA-ACTININ AND PXN.
[8]	"TES is a novel focal adhesion protein with a role in cell spreading." Coutts A.S., MacKenzie E., Griffith E., Black D.M. J. Cell Sci. 116:897-906(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZYX; GRIP1; ENAH AND TALIN, SUBCELLULAR LOCATION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"Tes, a specific Mena interacting partner, breaks the rules for EVH1 binding." Boeda B., Briggs D.C., Higgins T., Garvalov B.K., Fadden A.J., McDonald N.Q., Way M. Mol. Cell 28:1071-1082(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 357-421 IN COMPLEX WITH ZINC IONS AND ENAH.
[11]	"Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A." Boeda B., Knowles P.P., Briggs D.C., Murray-Rust J., Soriano E., Garvalov B.K., McDonald N.Q., Way M. J. Biol. Chem. 286:11543-11554(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.62 ANGSTROMS) OF 296-421 IN COMPLEX WITH ZINC IONS; ENAH AND ACTL7A, MUTAGENESIS OF CYS-328, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF260225 Genomic DNA. Translation: AAG17635.1.
AF260225 Genomic DNA. Translation: AAG17636.1.
AF245356 mRNA. Translation: AAG17612.1.
AF245357 mRNA. Translation: AAG17613.1.
AJ250865 mRNA. Translation: CAB65119.1.
AK021575 mRNA. Translation: BAB13846.1.
AK291802 mRNA. Translation: BAF84491.1.
AC073130 Genomic DNA. Translation: AAQ93367.1.
CH236947 Genomic DNA. Translation: EAL24365.1.
BC001451 mRNA. Translation: AAH01451.1.

IPI

IPI00024097.
IPI00216425.

RefSeq

NP_056456.1. NM_015641.3.
NP_690042.1. NM_152829.2.

UniGene

Hs.592286.
Hs.664957.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IYB	X-ray	2.35	E/F/G/H	357-421	[»]
2XQN	X-ray	2.62	T	296-421	[»]

ProteinModelPortal

Q9UGI8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UGI8. 18 interactions.

MINT

MINT-2821159.

STRING

9606.ENSP00000350937.

PTM databases

PhosphoSite

Q9UGI8.

Polymorphism databases

DMDM

17380320.

Proteomic databases

PaxDb

Q9UGI8.

PeptideAtlas

Q9UGI8.

PRIDE

Q9UGI8.

Protocols and materials databases

DNASU

26136.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000358204; ENSP00000350937; ENSG00000135269.
ENST00000393481; ENSP00000377121; ENSG00000135269.

GeneID

26136.

KEGG

hsa:26136.

UCSC

uc003vho.3. human.

Organism-specific databases

CTD

26136.

GeneCards

GC07P115850.

HGNC

HGNC:14620. TES.

HPA

CAB003690.
HPA015269.
HPA018123.

MIM

606085. gene.

neXtProt

NX_Q9UGI8.

PharmGKB

PA37906.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG272178.

HOGENOM

HOG000231628.

HOVERGEN

HBG001038.

InParanoid

Q9UGI8.

OMA

NNFNWHA.

OrthoDB

EOG4P8FK5.

PhylomeDB

Q9UGI8.

Gene expression databases

ArrayExpress

Q9UGI8.

Bgee

Q9UGI8.

CleanEx

HS_TES.

Genevestigator

Q9UGI8.

GermOnline

ENSG00000135269. Homo sapiens.

Family and domain databases

Gene3D

2.10.110.10. 3 hits.

InterPro

IPR010442. PET_domain.
IPR001781. Znf_LIM.
[Graphical view]

Pfam

PF00412. LIM. 3 hits.
PF06297. PET. 1 hit.
[Graphical view]

SMART

SM00132. LIM. 3 hits.
[Graphical view]

PROSITE

PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TES. human.

EvolutionaryTrace

Q9UGI8.

GenomeRNAi

26136.

NextBio

48175.

SOURCE

Search...

Entry information

Entry name

TES_HUMAN

Accession

Primary (citable) accession number: Q9UGI8
Secondary accession number(s): A4D0U6, Q9GZQ1, Q9HAJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.