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Q9UGI8 (TES_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testin
Alternative name(s):
TESS
Gene names
Name:TES
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. Ref.2 Ref.7 Ref.8

Subunit structure

Interacts via LIM domain 1 with ZYX. Interacts (via LIM domain 3) with ENAH and VASP. Interacts with ALKBH4, talin, actin, alpha-actinin, GRIP1 and PXN. Interacts (via LIM domain 2) with ACTL7A (via N-terminus). Heterodimer with ACTL7A; the heterodimer interacts with ENAH to form a heterotrimer. Ref.7 Ref.8 Ref.10 Ref.12

Subcellular location

Cytoplasm. Cell junctionfocal adhesion. Note: Detected along actin stress fibers. Ref.7 Ref.8

Tissue specificity

Ubiquitous. Ref.2

Domain

The N-terminal and the C-terminal halves of the protein can associate with each other, thereby hindering interactions with ZYX. Ref.7

Sequence similarities

Belongs to the prickle / espinas / testin family.

Contains 3 LIM zinc-binding domains.

Contains 1 PET domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UGI8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UGI8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Testin
PRO_0000075906

Regions

Domain92 – 199108PET
Domain234 – 29764LIM zinc-binding 1
Domain299 – 35961LIM zinc-binding 2
Domain362 – 42160LIM zinc-binding 3
Compositional bias22 – 4625Cys-rich

Natural variations

Alternative sequence1 – 99Missing in isoform 2.
VSP_003122
Natural variant2211A → V.
Corresponds to variant rs2272193 [ dbSNP | Ensembl ].
VAR_050170

Experimental info

Mutagenesis3281C → A: Abolishes interaction with ACTL7A. Ref.12
Mutagenesis3911C → A: Abolishes localization at focal adhesions. Ref.7
Sequence conflict1321K → E in BAB13846. Ref.3

Secondary structure

................................ 421
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AB9FF6669C50D492

FASTA42147,996
        10         20         30         40         50         60 
MDLENKVKKM GLGHEQGFGA PCLKCKEKCE GFELHFWRKI CRNCKCGQEE HDVLLSNEED 

        70         80         90        100        110        120 
RKVGKLFEDT KYTTLIAKLK SDGIPMYKRN VMILTNPVAA KKNVSINTVT YEWAPPVQNQ 

       130        140        150        160        170        180 
ALARQYMQML PKEKQPVAGS EGAQYRKKQL AKQLPAHDQD PSKCHELSPR EVKEMEQFVK 

       190        200        210        220        230        240 
KYKSEALGVG DVKLPCEMDA QGPKQMNIPG GDRSTPAAVG AMEDKSAEHK RTQYSCYCCK 

       250        260        270        280        290        300 
LSMKEGDPAI YAERAGYDKL WHPACFVCST CHELLVDMIY FWKNEKLYCG RHYCDSEKPR 

       310        320        330        340        350        360 
CAGCDELIFS NEYTQAENQN WHLKHFCCFD CDSILAGEIY VMVNDKPVCK PCYVKNHAVV 

       370        380        390        400        410        420 
CQGCHNAIDP EVQRVTYNNF SWHASTECFL CSCCSKCLIG QKFMPVEGMV FCSVECKKRM 


S 

« Hide

Isoform 2 [UniParc].

Checksum: C97A6D97364B3569
Show »

FASTA41246,910

References

« Hide 'large scale' references
[1]"Characterization of the human TESTIN gene localized in the FRA7G region at 7q31.2."
Tatarelli C., Linnenbach A., Mimori K., Croce C.M.
Genomics 68:1-12(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
[2]"The TES gene at 7q31.1 is methylated in tumours and encodes a novel growth-suppressing LIM domain protein."
Tobias E.S., Hurlstone A.F.L., MacKenzie E., McFarlane R., Black D.M.
Oncogene 20:2844-2853(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Embryo and Prostate.
[4]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[7]"The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions."
Garvalov B.K., Higgins T.E., Sutherland J.D., Zettl M., Scaplehorn N., Koecher T., Piddini E., Griffiths G., Way M.
J. Cell Biol. 161:33-39(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF CYS-391, INTERACTION WITH ENAH; ZYX; VASP; ACTIN FIBERS; ALPHA-ACTININ AND PXN.
[8]"TES is a novel focal adhesion protein with a role in cell spreading."
Coutts A.S., MacKenzie E., Griffith E., Black D.M.
J. Cell Sci. 116:897-906(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ZYX; GRIP1; ENAH AND TALIN, SUBCELLULAR LOCATION.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Human ALKBH4 interacts with proteins associated with transcription."
Bjornstad L.G., Meza T.J., Otterlei M., Olafsrud S.M., Meza-Zepeda L.A., Falnes P.O.
PLoS ONE 7:E49045-E49045(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALKBH4.
[11]"Tes, a specific Mena interacting partner, breaks the rules for EVH1 binding."
Boeda B., Briggs D.C., Higgins T., Garvalov B.K., Fadden A.J., McDonald N.Q., Way M.
Mol. Cell 28:1071-1082(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 357-421 IN COMPLEX WITH ZINC IONS AND ENAH.
[12]"Molecular recognition of the Tes LIM2-3 domains by the actin-related protein Arp7A."
Boeda B., Knowles P.P., Briggs D.C., Murray-Rust J., Soriano E., Garvalov B.K., McDonald N.Q., Way M.
J. Biol. Chem. 286:11543-11554(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.62 ANGSTROMS) OF 296-421 IN COMPLEX WITH ZINC IONS; ENAH AND ACTL7A, MUTAGENESIS OF CYS-328, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF260225 Genomic DNA. Translation: AAG17635.1.
AF260225 Genomic DNA. Translation: AAG17636.1.
AF245356 mRNA. Translation: AAG17612.1.
AF245357 mRNA. Translation: AAG17613.1.
AJ250865 mRNA. Translation: CAB65119.1.
AK021575 mRNA. Translation: BAB13846.1.
AK291802 mRNA. Translation: BAF84491.1.
AC073130 Genomic DNA. Translation: AAQ93367.1.
CH236947 Genomic DNA. Translation: EAL24365.1.
BC001451 mRNA. Translation: AAH01451.1.
CCDSCCDS5763.1. [Q9UGI8-1]
CCDS5764.1. [Q9UGI8-2]
RefSeqNP_056456.1. NM_015641.3. [Q9UGI8-1]
NP_690042.1. NM_152829.2. [Q9UGI8-2]
UniGeneHs.592286.
Hs.664957.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IYBX-ray2.35E/F/G/H357-421[»]
2XQNX-ray2.62T296-421[»]
ProteinModelPortalQ9UGI8.
SMRQ9UGI8. Positions 233-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117572. 23 interactions.
IntActQ9UGI8. 18 interactions.
MINTMINT-2821159.
STRING9606.ENSP00000350937.

PTM databases

PhosphoSiteQ9UGI8.

Polymorphism databases

DMDM17380320.

Proteomic databases

MaxQBQ9UGI8.
PaxDbQ9UGI8.
PeptideAtlasQ9UGI8.
PRIDEQ9UGI8.

Protocols and materials databases

DNASU26136.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000358204; ENSP00000350937; ENSG00000135269. [Q9UGI8-1]
ENST00000393481; ENSP00000377121; ENSG00000135269. [Q9UGI8-2]
GeneID26136.
KEGGhsa:26136.
UCSCuc003vho.3. human. [Q9UGI8-1]

Organism-specific databases

CTD26136.
GeneCardsGC07P115850.
HGNCHGNC:14620. TES.
HPACAB003690.
HPA015269.
HPA018123.
MIM606085. gene.
neXtProtNX_Q9UGI8.
PharmGKBPA37906.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG272178.
HOGENOMHOG000231628.
HOVERGENHBG001038.
InParanoidQ9UGI8.
OMAVKLPREM.
OrthoDBEOG7P8P7M.
PhylomeDBQ9UGI8.
TreeFamTF313265.

Gene expression databases

ArrayExpressQ9UGI8.
BgeeQ9UGI8.
CleanExHS_TES.
GenevestigatorQ9UGI8.

Family and domain databases

Gene3D2.10.110.10. 3 hits.
InterProIPR010442. PET_domain.
IPR027683. Testin.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERPTHR24211:SF1. PTHR24211:SF1. 1 hit.
PfamPF00412. LIM. 2 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTSM00132. LIM. 3 hits.
[Graphical view]
PROSITEPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTES. human.
EvolutionaryTraceQ9UGI8.
GeneWikiTestin.
GenomeRNAi26136.
NextBio48175.
PROQ9UGI8.
SOURCESearch...

Entry information

Entry nameTES_HUMAN
AccessionPrimary (citable) accession number: Q9UGI8
Secondary accession number(s): A4D0U6, Q9GZQ1, Q9HAJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM