Q9UGH3 (S23A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 23 member 2

Alternative name(s):
Na(+)/L-ascorbic acid transporter 2
Nucleobase transporter-like 1 protein
Sodium-dependent vitamin C transporter 2
Short name=hSVCT2
Yolk sac permease-like molecule 2

Gene names

Name:	SLC23A2
Synonyms:	KIAA0238, NBTL1, SLC23A1, SVCT2, YSPL2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each ascorbate.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous.
Post-translational modification	Phosphorylated Probable.
Sequence similarities	Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. [View classification]
Sequence caution	The sequence BAA13244.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-ascorbic acid metabolic process Non-traceable author statement Ref.3. Source: UniProtKB molecular hydrogen transport Non-traceable author statement Ref.2. Source: UniProtKB nucleobase-containing compound metabolic process Traceable author statement Ref.1. Source: ProtInc response to oxidative stress Inferred from electronic annotation. Source: Compara transepithelial L-ascorbic acid transport Inferred from direct assay PubMed 18417304. Source: UniProtKB
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 18417304. Source: UniProtKB basal plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from direct assay PubMed 15993839PubMed 18247577PubMed 18417304PubMed 19216494. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	L-ascorbate:sodium symporter activity Inferred from electronic annotation. Source: Compara nucleobase transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc sodium-dependent L-ascorbate transmembrane transporter activity Inferred from direct assay PubMed 10631088PubMed 15993839. Source: UniProtKB sodium-dependent multivitamin transmembrane transporter activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Solute carrier family 23 member 2		PRO_0000165978
Regions
	Transmembrane	103 – 123	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Transmembrane	219 – 239	21	Helical; Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Transmembrane	267 – 287	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	372 – 392	21	Helical; Potential
	Transmembrane	462 – 482	21	Helical; Potential
	Transmembrane	486 – 506	21	Helical; Potential
	Transmembrane	514 – 534	21	Helical; Potential
	Transmembrane	548 – 568	21	Helical; Potential
Amino acid modifications
	Modified residue	78	1	Phosphoserine By similarity
	Modified residue	79	1	Phosphothreonine By similarity
	Modified residue	81	1	Phosphoserine Ref.11
	Modified residue	649	1	Phosphothreonine By similarity
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	30	1	P → R in AAD11783. Ref.4
	Sequence conflict	141	1	I → T in AAD11783. Ref.4
	Sequence conflict	167	1	A → T in AAC78806. Ref.1
	Sequence conflict	182	1	L → S in AAD11783. Ref.4
	Sequence conflict	215	1	I → V in AAD11783. Ref.4
	Sequence conflict	314	1	F → L in CAC83100. Ref.5
	Sequence conflict	418 – 424	7	GIFVEGL → YVPEKTS Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q9UGH3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 28C9E190D9528D39	FASTA	650	70,337
	10 20 30 40 50 60 MMGIGKNTTS KSMEAGSSTE GKYEDEAKHP AFFTLPVVIN GGATSSGEQD NEDTELMAIY 70 80 90 100 110 120 TTENGIAEKS SLAETLDSTG SLDPQRSDMI YTIEDVPPWY LCIFLGLQHY LTCFSGTIAV 130 140 150 160 170 180 PFLLADAMCV GYDQWATSQL IGTIFFCVGI TTLLQTTFGC RLPLFQASAF AFLAPARAIL 190 200 210 220 230 240 SLDKWKCNTT DVSVANGTAE LLHTEHIWYP RIREIQGAII MSSLIEVVIG LLGLPGALLK 250 260 270 280 290 300 YIGPLTITPT VALIGLSGFQ AAGERAGKHW GIAMLTIFLV LLFSQYARNV KFPLPIYKSK 310 320 330 340 350 360 KGWTAYKLQL FKMFPIILAI LVSWLLCFIF TVTDVFPPDS TKYGFYARTD ARQGVLLVAP 370 380 390 400 410 420 WFKVPYPFQW GLPTVSAAGV IGMLSAVVAS IIESIGDYYA CARLSCAPPP PIHAINRGIF 430 440 450 460 470 480 VEGLSCVLDG IFGTGNGSTS SSPNIGVLGI TKVGSRRVIQ CGAALMLALG MIGKFSALFA 490 500 510 520 530 540 SLPDPVLGAL FCTLFGMITA VGLSNLQFID LNSSRNLFVL GFSIFFGLVL PSYLRQNPLV 550 560 570 580 590 600 TGITGIDQVL NVLLTTAMFV GGCVAFILDN TIPGTPEERG IRKWKKGVGK GNKSLDGMES 610 620 630 640 650 YNLPFGMNII KKYRCFSYLP ISPTFVGYTW KGLRKSDNSR SSDEDSQATG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters." Faaland C.A., Race J.E., Ricken G., Warner F.J., Williams W.J., Holtzman E.J. Biochim. Biophys. Acta 1442:353-360(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Human placental sodium-dependent vitamin C transporter (SVCT2): molecular cloning and transport function." Rajan D.P., Huang W., Dutta B., Devoe L.D., Leibach F.H., Ganapathy V., Prasad P.D. Biochem. Biophys. Res. Commun. 262:762-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Choriocarcinoma.
[3]	"Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2." Daruwala R.C., Song J., Koh W.S., Rumsey S.C., Levine M. FEBS Lett. 460:480-484(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[4]	"A human nucleobase transporter-like cDNA (SLC23A1): member of a transporter family conserved from bacteria to mammals." Hogue D.L., Ling V. Genomics 59:18-23(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary carcinoma.
[5]	"Vitamin C transport systems of mammalian cells." Liang W.J., Johnson D., Jarvis S.M. Mol. Membr. Biol. 18:87-95(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[6]	"SVCT2 in ciliated human tracheal epithelial cultures." Schwarzer C., Borcanski G., Widdicombe J., Fischer H., Illek B. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Trachea.
[7]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[8]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[9]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-424. Tissue: Skin.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058319 mRNA. Translation: AAC78806.1. AJ269478 mRNA. Translation: CAB58120.1. AF164142 mRNA. Translation: AAF80493.1. AF092511 mRNA. Translation: AAD11783.1. AJ292318 mRNA. Translation: CAC83100.1. AY380556 mRNA. Translation: AAQ79775.1. D87075 mRNA. Translation: BAA13244.2. Different initiation. AL389886 Genomic DNA. Translation: CAC16126.1. BC013112 mRNA. Translation: AAH13112.1.
IPI	IPI00295774.
PIR	JC7095.
RefSeq	NP_005107.4. NM_005116.5. NP_976072.1. NM_203327.1.
UniGene	Hs.516866.
3D structure databases
ProteinModelPortal	Q9UGH3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UGH3. 1 interaction.
Protein family/group databases
TCDB	2.A.40.6.2. nucleobase:cation symporter-2 (NCS2) family.
PTM databases
PhosphoSite	Q9UGH3.
Polymorphism databases
DMDM	24212469.
Proteomic databases
PaxDb	Q9UGH3.
PRIDE	Q9UGH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338244; ENSP00000344322; ENSG00000089057. ENST00000379333; ENSP00000368637; ENSG00000089057.
GeneID	9962.
KEGG	hsa:9962.
UCSC	uc002wlg.1. human.
Organism-specific databases
CTD	9962.
GeneCards	GC20M004781.
HGNC	HGNC:10973. SLC23A2.
HPA	HPA052825.
MIM	603791. gene.
neXtProt	NX_Q9UGH3.
PharmGKB	PA35849.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2233.
HOVERGEN	HBG056256.
InParanoid	Q9UGH3.
KO	K14611.
OMA	LEKWKCN.
OrthoDB	EOG4JHCF8.
PhylomeDB	Q9UGH3.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9UGH3.
Bgee	Q9UGH3.
CleanEx	HS_SLC23A1. HS_SLC23A2.
Genevestigator	Q9UGH3.
GermOnline	ENSG00000089057. Homo sapiens.
Family and domain databases
InterPro	IPR006042. Xan_ur_permease. IPR006043. Xant/urac/vitC. [Graphical view]
PANTHER	PTHR11119. PTHR11119. 1 hit.
Pfam	PF00860. Xan_ur_permease. 1 hit. [Graphical view]
PROSITE	PS01116. XANTH_URACIL_PERMASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL3271.
ChiTaRS	SLC23A2. human.
GenomeRNAi	9962.
NextBio	37592.
SOURCE	Search...

Entry information

Entry name

S23A2_HUMAN

Accession

Primary (citable) accession number: Q9UGH3
Secondary accession number(s): Q8WWR4 , Q92512, Q96D54, Q9UNU1, Q9UP85

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.