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Protein

Inositol oxygenase

Gene

MIOX

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.1 Publication

Cofactori

Fe cation1 PublicationNote: Binds 2 iron ions per subunit.1 Publication

Pathwayi: myo-inositol degradation into D-glucuronate

This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol.
Proteins known to be involved in this subpathway in this organism are:
  1. Inositol oxygenase (MIOX)
This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29SubstrateBy similarity1
Metal bindingi98Iron 11 Publication1
Metal bindingi123Iron 11 Publication1
Metal bindingi124Iron 11 Publication1
Metal bindingi124Iron 21 Publication1
Binding sitei127Substrate1
Metal bindingi194Iron 21 Publication1
Metal bindingi220Iron 21 Publication1
Metal bindingi253Iron 11 Publication1

GO - Molecular functioni

GO - Biological processi

  • inositol catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS02017-MONOMER.
BRENDAi1.13.99.1. 2681.
SABIO-RKQ9UGB7.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase (EC:1.13.99.1)
Alternative name(s):
Aldehyde reductase-like 6
Kidney-specific protein 32
Myo-inositol oxygenase
Short name:
MI oxygenase
Renal-specific oxidoreductase
Gene namesi
Name:MIOX
Synonyms:ALDRL6, KSP32, RSOR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:14522. MIOX.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • inclusion body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127K → S: Strongly reduced enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi55586.
OpenTargetsiENSG00000100253.
PharmGKBiPA24716.

Polymorphism and mutation databases

BioMutaiMIOX.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000791481 – 285Inositol oxygenaseAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9UGB7.
PeptideAtlasiQ9UGB7.
PRIDEiQ9UGB7.
TopDownProteomicsiQ9UGB7-1. [Q9UGB7-1]

PTM databases

iPTMnetiQ9UGB7.
PhosphoSitePlusiQ9UGB7.

Expressioni

Tissue specificityi

Kidney specific.

Gene expression databases

BgeeiENSG00000100253.
CleanExiHS_MIOX.
ExpressionAtlasiQ9UGB7. baseline and differential.
GenevisibleiQ9UGB7. HS.

Organism-specific databases

HPAiHPA039451.
HPA039562.

Interactioni

Protein-protein interaction databases

BioGridi120733. 1 interactor.
STRINGi9606.ENSP00000216075.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 50Combined sources13
Helixi53 – 63Combined sources11
Beta strandi68 – 71Combined sources4
Helixi73 – 79Combined sources7
Helixi80 – 82Combined sources3
Helixi95 – 109Combined sources15
Helixi114 – 122Combined sources9
Helixi125 – 127Combined sources3
Helixi128 – 131Combined sources4
Helixi136 – 138Combined sources3
Beta strandi145 – 148Combined sources4
Helixi157 – 160Combined sources4
Helixi165 – 168Combined sources4
Turni170 – 172Combined sources3
Beta strandi173 – 176Combined sources4
Helixi185 – 187Combined sources3
Helixi194 – 205Combined sources12
Helixi211 – 219Combined sources9
Helixi223 – 226Combined sources4
Turni232 – 234Combined sources3
Helixi237 – 253Combined sources17
Helixi264 – 278Combined sources15
Beta strandi283 – 285Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IBNX-ray1.50A/B38-285[»]
ProteinModelPortaliQ9UGB7.
SMRiQ9UGB7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UGB7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 87Substrate binding3
Regioni141 – 142Substrate binding2
Regioni220 – 221Substrate binding2

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiKOG1573. Eukaryota.
ENOG410XQ4J. LUCA.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9UGB7.
KOiK00469.
OMAiNFYREQH.
OrthoDBiEOG091G0FIX.
PhylomeDBiQ9UGB7.
TreeFamiTF300089.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. MIOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UGB7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVTVGPDPS LVYRPDVDPE VAKDKASFRN YTSGPLLDRV FTTYKLMHTH
60 70 80 90 100
QTVDFVRSKH AQFGGFSYKK MTVMEAVDLL DGLVDESDPD VDFPNSFHAF
110 120 130 140 150
QTAEGIRKAH PDKDWFHLVG LLHDLGKVLA LFGEPQWAVV GDTFPVGCRP
160 170 180 190 200
QASVVFCDST FQDNPDLQDP RYSTELGMYQ PHCGLDRVLM SWGHDEYMYQ
210 220 230 240 250
VMKFNKFSLP PEAFYMIRFH SFYPWHTGRD YQQLCSQQDL AMLPWVREFN
260 270 280
KFDLYTKCPD LPDVDKLRPY YQGLIDKYCP GILSW
Length:285
Mass (Da):33,010
Last modified:May 1, 2000 - v1
Checksum:iED70B197FF267B2B
GO
Isoform 2 (identifier: Q9UGB7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-231: DWFHLVGLLH...FYPWHTGRDY → VPNLPCPSPS...QAAALLPGAH
     232-285: Missing.

Show »
Length:231
Mass (Da):24,516
Checksum:i4C63EF35422491AF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4T → D in AAF25204 (PubMed:10944187).Curated1
Sequence conflicti199Y → F in AAK00766 (Ref. 2) Curated1
Sequence conflicti219 – 221FHS → VHF in AAK00766 (Ref. 2) Curated3
Sequence conflicti282I → T in AAF25204 (PubMed:10944187).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041667114 – 231DWFHL…TGRDY → VPNLPCPSPSQTGSTSSGSC TTWGRSWPCSGSPSGLSSAT PSPSDAVRRPPWFSATPPSR TTLTSRILDTAQSSGCISPT VGSTGSSCPGAMMQVRPLHQ VPGPAGRGQAAALLPGAH in isoform 2. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_041668232 – 285Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY064416 mRNA. Translation: AAL47192.1.
AF230095 mRNA. Translation: AAK00766.1.
AF197129 mRNA. Translation: AAF25204.1.
AY738258 mRNA. Translation: AAV65816.1.
AK000576 mRNA. Translation: BAA91266.1.
CR456478 mRNA. Translation: CAG30364.1.
AL096767 Genomic DNA. Translation: CAB63064.1.
AL096767 Genomic DNA. Translation: CAO03465.1.
CH471138 Genomic DNA. Translation: EAW73547.1.
BC012025 mRNA. Translation: AAH12025.1.
BC073848 mRNA. Translation: AAH73848.1.
CCDSiCCDS14092.1. [Q9UGB7-1]
RefSeqiNP_060054.4. NM_017584.5. [Q9UGB7-1]
UniGeneiHs.129227.

Genome annotation databases

EnsembliENST00000216075; ENSP00000216075; ENSG00000100253. [Q9UGB7-1]
ENST00000395733; ENSP00000379082; ENSG00000100253. [Q9UGB7-2]
GeneIDi55586.
KEGGihsa:55586.
UCSCiuc003bll.2. human. [Q9UGB7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY064416 mRNA. Translation: AAL47192.1.
AF230095 mRNA. Translation: AAK00766.1.
AF197129 mRNA. Translation: AAF25204.1.
AY738258 mRNA. Translation: AAV65816.1.
AK000576 mRNA. Translation: BAA91266.1.
CR456478 mRNA. Translation: CAG30364.1.
AL096767 Genomic DNA. Translation: CAB63064.1.
AL096767 Genomic DNA. Translation: CAO03465.1.
CH471138 Genomic DNA. Translation: EAW73547.1.
BC012025 mRNA. Translation: AAH12025.1.
BC073848 mRNA. Translation: AAH73848.1.
CCDSiCCDS14092.1. [Q9UGB7-1]
RefSeqiNP_060054.4. NM_017584.5. [Q9UGB7-1]
UniGeneiHs.129227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IBNX-ray1.50A/B38-285[»]
ProteinModelPortaliQ9UGB7.
SMRiQ9UGB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120733. 1 interactor.
STRINGi9606.ENSP00000216075.

PTM databases

iPTMnetiQ9UGB7.
PhosphoSitePlusiQ9UGB7.

Polymorphism and mutation databases

BioMutaiMIOX.

Proteomic databases

PaxDbiQ9UGB7.
PeptideAtlasiQ9UGB7.
PRIDEiQ9UGB7.
TopDownProteomicsiQ9UGB7-1. [Q9UGB7-1]

Protocols and materials databases

DNASUi55586.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216075; ENSP00000216075; ENSG00000100253. [Q9UGB7-1]
ENST00000395733; ENSP00000379082; ENSG00000100253. [Q9UGB7-2]
GeneIDi55586.
KEGGihsa:55586.
UCSCiuc003bll.2. human. [Q9UGB7-1]

Organism-specific databases

CTDi55586.
DisGeNETi55586.
GeneCardsiMIOX.
HGNCiHGNC:14522. MIOX.
HPAiHPA039451.
HPA039562.
MIMi606774. gene.
neXtProtiNX_Q9UGB7.
OpenTargetsiENSG00000100253.
PharmGKBiPA24716.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1573. Eukaryota.
ENOG410XQ4J. LUCA.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9UGB7.
KOiK00469.
OMAiNFYREQH.
OrthoDBiEOG091G0FIX.
PhylomeDBiQ9UGB7.
TreeFamiTF300089.

Enzyme and pathway databases

UniPathwayiUPA00111; UER00527.
BioCyciZFISH:HS02017-MONOMER.
BRENDAi1.13.99.1. 2681.
SABIO-RKQ9UGB7.

Miscellaneous databases

ChiTaRSiMIOX. human.
EvolutionaryTraceiQ9UGB7.
GenomeRNAii55586.
PROiQ9UGB7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100253.
CleanExiHS_MIOX.
ExpressionAtlasiQ9UGB7. baseline and differential.
GenevisibleiQ9UGB7. HS.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. MIOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIOX_HUMAN
AccessioniPrimary (citable) accession number: Q9UGB7
Secondary accession number(s): Q05DJ6
, Q5S8C9, Q9BZZ1, Q9UHB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.