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Protein

Intraflagellar transport protein 172 homolog

Gene

IFT172

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138002-MONOMER.
ReactomeiR-HSA-5610787. Hedgehog 'off' state.
R-HSA-5620924. Intraflagellar transport.
SIGNORiQ9UG01.

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 172 homolog
Gene namesi
Name:IFT172
Synonyms:KIAA1179
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30391. IFT172.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

Pathology & Biotechi

Involvement in diseasei

Short-rib thoracic dysplasia 10 with or without polydactyly (SRTD10)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of short-rib thoracic dysplasia, a group of autosomal recessive ciliopathies that are characterized by a constricted thoracic cage, short ribs, shortened tubular bones, and a 'trident' appearance of the acetabular roof. Polydactyly is variably present. Non-skeletal involvement can include cleft lip/palate as well as anomalies of major organs such as the brain, eye, heart, kidneys, liver, pancreas, intestines, and genitalia. Some forms of the disease are lethal in the neonatal period due to respiratory insufficiency secondary to a severely restricted thoracic cage, whereas others are compatible with life. Disease spectrum encompasses Ellis-van Creveld syndrome, asphyxiating thoracic dystrophy (Jeune syndrome), Mainzer-Saldino syndrome, and short rib-polydactyly syndrome.
See also OMIM:615630
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_070956296R → W in SRTD10. 1 PublicationCorresponds to variant rs145541911dbSNPEnsembl.1
Natural variantiVAR_070957411I → N in SRTD10. 1 PublicationCorresponds to variant rs587777085dbSNPEnsembl.1
Natural variantiVAR_070958464 – 465Missing in SRTD10. 1 Publication2
Natural variantiVAR_0709591536L → P in SRTD10. 1 PublicationCorresponds to variant rs587777080dbSNPEnsembl.1
Natural variantiVAR_0709601544R → C in SRTD10. 1 PublicationCorresponds to variant rs587777079dbSNPEnsembl.1
Natural variantiVAR_0709611727C → R in SRTD10. 1 PublicationCorresponds to variant rs149614625dbSNPEnsembl.1
Retinitis pigmentosa 71 (RP71)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
See also OMIM:616394
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_073800257L → P in RP71; represents a null allele. 1 PublicationCorresponds to variant rs786205857dbSNPEnsembl.1
Natural variantiVAR_0738011567H → Q in RP71; hypomorphic mutation. 1 PublicationCorresponds to variant rs786205855dbSNPEnsembl.1
Natural variantiVAR_0738021605D → E in RP71; hypomorphic mutation. 1 PublicationCorresponds to variant rs786205856dbSNPEnsembl.1

Keywords - Diseasei

Ciliopathy, Disease mutation, Retinitis pigmentosa

Organism-specific databases

DisGeNETi26160.
MalaCardsiIFT172.
MIMi615630. phenotype.
616394. phenotype.
OpenTargetsiENSG00000138002.
Orphaneti474. Jeune syndrome.
140969. Saldino-Mainzer syndrome.
PharmGKBiPA142671666.

Polymorphism and mutation databases

BioMutaiIFT172.
DMDMi182662418.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289411 – 1749Intraflagellar transport protein 172 homologAdd BLAST1749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Cross-linki4Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)
Modified residuei672Omega-N-methylarginineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

EPDiQ9UG01.
PaxDbiQ9UG01.
PeptideAtlasiQ9UG01.
PRIDEiQ9UG01.

PTM databases

iPTMnetiQ9UG01.
PhosphoSitePlusiQ9UG01.

Expressioni

Gene expression databases

BgeeiENSG00000138002.
CleanExiHS_IFT172.
ExpressionAtlasiQ9UG01. baseline and differential.
GenevisibleiQ9UG01. HS.

Organism-specific databases

HPAiHPA044893.

Interactioni

Subunit structurei

Interacts with IFT88. Interacts with RABL2/RABL2A; binds preferentially to GDP-bound RABL2.By similarity

Protein-protein interaction databases

BioGridi117589. 13 interactors.
IntActiQ9UG01. 24 interactors.
STRINGi9606.ENSP00000260570.

Structurei

3D structure databases

ProteinModelPortaliQ9UG01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 53WD 1Add BLAST40
Repeati64 – 103WD 2Add BLAST40
Repeati110 – 148WD 3Add BLAST39
Repeati150 – 191WD 4Add BLAST42
Repeati195 – 233WD 5Add BLAST39
Repeati238 – 278WD 6Add BLAST41
Repeati284 – 323WD 7Add BLAST40
Repeati483 – 520WD 8Add BLAST38
Repeati521 – 559WD 9Add BLAST39
Repeati593 – 624TPR 1Add BLAST32
Repeati692 – 725TPR 2Add BLAST34
Repeati809 – 842TPR 3Add BLAST34
Repeati854 – 887TPR 4Add BLAST34
Repeati912 – 945TPR 5Add BLAST34
Repeati947 – 970TPR 6Add BLAST24
Repeati971 – 1004TPR 7Add BLAST34
Repeati1042 – 1075TPR 8Add BLAST34
Repeati1142 – 1175TPR 9Add BLAST34
Repeati1276 – 1309TPR 10Add BLAST34
Repeati1345 – 1378TPR 11Add BLAST34
Repeati1411 – 1445TPR 12Add BLAST35
Repeati1447 – 1477TPR 13Add BLAST31
Repeati1574 – 1607TPR 14Add BLAST34

Sequence similaritiesi

Belongs to the IFT172 family.Curated
Contains 14 TPR repeats.Curated
Contains 9 WD repeats.Curated

Keywords - Domaini

Repeat, TPR repeat, WD repeat

Phylogenomic databases

eggNOGiKOG3616. Eukaryota.
ENOG410XR2C. LUCA.
GeneTreeiENSGT00440000033648.
HOGENOMiHOG000286003.
HOVERGENiHBG098449.
InParanoidiQ9UG01.
KOiK19676.
OMAiEDIHLKN.
OrthoDBiEOG091G02TF.
PhylomeDBiQ9UG01.
TreeFamiTF312901.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR011990. TPR-like_helical_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF48452. SSF48452. 2 hits.
SSF50978. SSF50978. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UG01-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLKHLRTLL SPQDGAAKVT CMAWSQNNAK FAVCTVDRVV LLYDEHGERR
60 70 80 90 100
DKFSTKPADM KYGRKSYMVK GMAFSPDSTK IAIGQTDNII YVYKIGEDWG
110 120 130 140 150
DKKVICNKFI QTSAVTCLQW PAEYIIVFGL AEGKVRLANT KTNKSSTIYG
160 170 180 190 200
TESYVVSLTT NCSGKGILSG HADGTIVRYF FDDEGSGESQ GKLVNHPCPP
210 220 230 240 250
YALAWATNSI VAAGCDRKIV AYGKEGHMLQ TFDYSRDPQE REFTTAVSSP
260 270 280 290 300
GGQSVVLGSY DRLRVFNWIP RRSIWEEAKP KEITNLYTIT ALAWKRDGSR
310 320 330 340 350
LCVGTLCGGV EQFDCCLRRS IYKNKFELTY VGPSQVIVKN LSSGTRVVLK
360 370 380 390 400
SHYGYEVEEV KILGKERYLV AHTSETLLLG DLNTNRLSEI AWQGSGGNEK
410 420 430 440 450
YFFENENVCM IFNAGELTLV EYGNNDTLGS VRTEFMNPHL ISVRINERCQ
460 470 480 490 500
RGTEDNKKLA YLIDIKTIAI VDLIGGYNIG TVSHESRVDW LELNETGHKL
510 520 530 540 550
LFRDRKLRLH LYDIESCSKT MILNFCSYMQ WVPGSDVLVA QNRNSLCVWY
560 570 580 590 600
NIEAPERVTM FTIRGDVIGL ERGGGKTEVM VMEGVTTVAY TLDEGLIEFG
610 620 630 640 650
TAIDDGNYIR ATAFLETLEM TPETEAMWKT LSKLALEARQ LHIAERCFSA
660 670 680 690 700
LGQVAKARFL HETNEIADQV SREYGGEGTD FYQVRARLAM LEKNYKLAEM
710 720 730 740 750
IFLEQNAVEE AMGMYQELHR WDECIAVAEA KGHPALEKLR RSYYQWLMDT
760 770 780 790 800
QQEERAGELQ ESQGDGLAAI SLYLKAGLPA KAARLVLTRE ELLANTELVE
810 820 830 840 850
HITAALIKGE LYERAGDLFE KIHNPQKALE CYRKGNAFMK AVELARLAFP
860 870 880 890 900
VEVVKLEEAW GDHLVQQKQL DAAINHYIEA RCSIKAIEAA LGARQWKKAI
910 920 930 940 950
YILDLQDRNT ASKYYPLVAQ HYASLQEYEI AEELYTKGDR TKDAIDMYTQ
960 970 980 990 1000
AGRWEQAHKL AMKCMRPEDV SVLYITQAQE MEKQGKYREA ERLYVTVQEP
1010 1020 1030 1040 1050
DLAITMYKKH KLYDDMIRLV GKHHPDLLSD THLHLGKELE AEGRLQEAEY
1060 1070 1080 1090 1100
HYLEAQEWKA TVNMYRASGL WEEAYRVART QGGANAHKHV AYLWAKSLGG
1110 1120 1130 1140 1150
EAAVRLLNKL GLLEAAVDHA ADNCSFEFAF ELSRLALKHK TPEVHLKYAM
1160 1170 1180 1190 1200
FLEDEGKFEE AEAEFIRAGK PKEAVLMFVH NQDWEAAQRV AEAHDPDSVA
1210 1220 1230 1240 1250
EVLVGQARGA LEEKDFQKAE GLLLRAQRPG LALNYYKEAG LWSDALRICK
1260 1270 1280 1290 1300
DYVPSQLEAL QEEYEREATK KGARGVEGFV EQARHWEQAG EYSRAVDCYL
1310 1320 1330 1340 1350
KVRDSGNSGL AEKCWMKAAE LSIKFLPPQR NMEVVLAVGP QLIGIGKHSA
1360 1370 1380 1390 1400
AAELYLNLDL VKEAIDAFIE GEEWNKAKRV AKELDPRYED YVDQHYKEFL
1410 1420 1430 1440 1450
KNQGKVDSLV GVDVIAALDL YVEQGQWDKC IETATKQNYK ILHKYVALYA
1460 1470 1480 1490 1500
THLIREGSSA QALALYVQHG APANPQNFNI YKRIFTDMVS SPGTNCAEAY
1510 1520 1530 1540 1550
HSWADLRDVL FNLCENLVKS SEANSPAHEE FKTMLLIAHY YATRSAAQSV
1560 1570 1580 1590 1600
KQLETVAARL SVSLLRHTQL LPVDKAFYEA GIAAKAVGWD NMAFIFLNRF
1610 1620 1630 1640 1650
LDLTDAIEEG TLDGLDHSDF QDTDIPFEVP LPAKQHVPEA EREEVRDWVL
1660 1670 1680 1690 1700
TVSMDQRLEQ VLPRDERGAY EASLVAASTG VRALPCLITG YPILRNKIEF
1710 1720 1730 1740
KRPGKAANKD NWNKFLMAIK TSHSPVCQDV LKFISQWCGG LPSTSFSFQ
Length:1,749
Mass (Da):197,576
Last modified:April 8, 2008 - v2
Checksum:i78AA83802D79E3CE
GO
Isoform 2 (identifier: Q9UG01-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1514-1525: CENLVKSSEANS → AVLSPSSSVKTW
     1526-1749: Missing.

Note: No experimental confirmation available.
Show »
Length:1,525
Mass (Da):172,438
Checksum:i0F5C5D89B95F6B0F
GO
Isoform 3 (identifier: Q9UG01-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     509-532: LHLYDIESCSKTMILNFCSYMQWV → VRRATKALGIGWPTEGVRQAATRD
     533-1749: Missing.

Note: No experimental confirmation available.
Show »
Length:532
Mass (Da):59,659
Checksum:i73321ACEDA74FF52
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1475P → L in CAB55914 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_073800257L → P in RP71; represents a null allele. 1 PublicationCorresponds to variant rs786205857dbSNPEnsembl.1
Natural variantiVAR_070956296R → W in SRTD10. 1 PublicationCorresponds to variant rs145541911dbSNPEnsembl.1
Natural variantiVAR_070957411I → N in SRTD10. 1 PublicationCorresponds to variant rs587777085dbSNPEnsembl.1
Natural variantiVAR_070958464 – 465Missing in SRTD10. 1 Publication2
Natural variantiVAR_042581953R → H.Corresponds to variant rs704793dbSNPEnsembl.1
Natural variantiVAR_0709591536L → P in SRTD10. 1 PublicationCorresponds to variant rs587777080dbSNPEnsembl.1
Natural variantiVAR_0709601544R → C in SRTD10. 1 PublicationCorresponds to variant rs587777079dbSNPEnsembl.1
Natural variantiVAR_0738011567H → Q in RP71; hypomorphic mutation. 1 PublicationCorresponds to variant rs786205855dbSNPEnsembl.1
Natural variantiVAR_0738021605D → E in RP71; hypomorphic mutation. 1 PublicationCorresponds to variant rs786205856dbSNPEnsembl.1
Natural variantiVAR_0709611727C → R in SRTD10. 1 PublicationCorresponds to variant rs149614625dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054428509 – 532LHLYD…YMQWV → VRRATKALGIGWPTEGVRQA ATRD in isoform 3. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_054429533 – 1749Missing in isoform 3. 1 PublicationAdd BLAST1217
Alternative sequenceiVSP_0328481514 – 1525CENLV…SEANS → AVLSPSSSVKTW in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_0328491526 – 1749Missing in isoform 2. 1 PublicationAdd BLAST224

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074117 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00573.1.
BC008024 mRNA. Translation: AAH08024.1.
BC047294 mRNA. Translation: AAH47294.1.
BC137126 mRNA. Translation: AAI37127.1.
BC142675 mRNA. Translation: AAI42676.1.
BC142729 mRNA. Translation: AAI42730.1.
AL110218 mRNA. Translation: CAB53678.1.
AL117421 mRNA. Translation: CAB55914.2.
AB033005 mRNA. Translation: BAA86493.1.
CCDSiCCDS1755.1. [Q9UG01-1]
PIRiT14758.
T17224.
RefSeqiNP_056477.1. NM_015662.2. [Q9UG01-1]
UniGeneiHs.127401.

Genome annotation databases

EnsembliENST00000260570; ENSP00000260570; ENSG00000138002. [Q9UG01-1]
ENST00000359466; ENSP00000352443; ENSG00000138002. [Q9UG01-3]
GeneIDi26160.
KEGGihsa:26160.
UCSCiuc002rku.4. human. [Q9UG01-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074117 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00573.1.
BC008024 mRNA. Translation: AAH08024.1.
BC047294 mRNA. Translation: AAH47294.1.
BC137126 mRNA. Translation: AAI37127.1.
BC142675 mRNA. Translation: AAI42676.1.
BC142729 mRNA. Translation: AAI42730.1.
AL110218 mRNA. Translation: CAB53678.1.
AL117421 mRNA. Translation: CAB55914.2.
AB033005 mRNA. Translation: BAA86493.1.
CCDSiCCDS1755.1. [Q9UG01-1]
PIRiT14758.
T17224.
RefSeqiNP_056477.1. NM_015662.2. [Q9UG01-1]
UniGeneiHs.127401.

3D structure databases

ProteinModelPortaliQ9UG01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117589. 13 interactors.
IntActiQ9UG01. 24 interactors.
STRINGi9606.ENSP00000260570.

PTM databases

iPTMnetiQ9UG01.
PhosphoSitePlusiQ9UG01.

Polymorphism and mutation databases

BioMutaiIFT172.
DMDMi182662418.

Proteomic databases

EPDiQ9UG01.
PaxDbiQ9UG01.
PeptideAtlasiQ9UG01.
PRIDEiQ9UG01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260570; ENSP00000260570; ENSG00000138002. [Q9UG01-1]
ENST00000359466; ENSP00000352443; ENSG00000138002. [Q9UG01-3]
GeneIDi26160.
KEGGihsa:26160.
UCSCiuc002rku.4. human. [Q9UG01-1]

Organism-specific databases

CTDi26160.
DisGeNETi26160.
GeneCardsiIFT172.
H-InvDBHIX0001924.
HIX0119024.
HGNCiHGNC:30391. IFT172.
HPAiHPA044893.
MalaCardsiIFT172.
MIMi607386. gene.
615630. phenotype.
616394. phenotype.
neXtProtiNX_Q9UG01.
OpenTargetsiENSG00000138002.
Orphaneti474. Jeune syndrome.
140969. Saldino-Mainzer syndrome.
PharmGKBiPA142671666.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3616. Eukaryota.
ENOG410XR2C. LUCA.
GeneTreeiENSGT00440000033648.
HOGENOMiHOG000286003.
HOVERGENiHBG098449.
InParanoidiQ9UG01.
KOiK19676.
OMAiEDIHLKN.
OrthoDBiEOG091G02TF.
PhylomeDBiQ9UG01.
TreeFamiTF312901.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138002-MONOMER.
ReactomeiR-HSA-5610787. Hedgehog 'off' state.
R-HSA-5620924. Intraflagellar transport.
SIGNORiQ9UG01.

Miscellaneous databases

ChiTaRSiIFT172. human.
GenomeRNAii26160.
PROiQ9UG01.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138002.
CleanExiHS_IFT172.
ExpressionAtlasiQ9UG01. baseline and differential.
GenevisibleiQ9UG01. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR011990. TPR-like_helical_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF48452. SSF48452. 2 hits.
SSF50978. SSF50978. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF172_HUMAN
AccessioniPrimary (citable) accession number: Q9UG01
Secondary accession number(s): A5PKZ0
, B2RNU5, Q86X44, Q96HW4, Q9UFJ9, Q9ULP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.