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Q9UF33 (EPHA6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ephrin type-A receptor 6

EC=2.7.10.1
Alternative name(s):
EPH homology kinase 2
Short name=EHK-2
EPH-like kinase 12
Short name=EK12
Gene names
Name:EPHA6
Synonyms:EHK2, HEK12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Expressed in brain and testis. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Contains 1 Eph LBD (Eph ligand-binding) domain.

Contains 2 fibronectin type-III domains.

Contains 1 protein kinase domain.

Contains 1 SAM (sterile alpha motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UF33-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UF33-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-513: Missing.
     514-536: IRVRTATGYSGYSQKFEFETGDE → MKDSPFQVTKLYWLNEKWDFIAS
     834-847: GGKIPIRWTAPEAI → DLFQTLTLNLCYSA
     848-1035: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 10351013Ephrin type-A receptor 6
PRO_0000235684

Regions

Topological domain23 – 549527Extracellular Potential
Transmembrane550 – 57021Helical; Potential
Topological domain571 – 1035465Cytoplasmic Potential
Domain33 – 211179Eph LBD
Domain330 – 440111Fibronectin type-III 1
Domain441 – 53696Fibronectin type-III 2
Domain630 – 943314Protein kinase
Domain960 – 102465SAM
Nucleotide binding636 – 6449ATP By similarity
Motif1033 – 10353PDZ-binding Potential

Sites

Active site7971Proton acceptor By similarity
Binding site6621ATP By similarity

Amino acid modifications

Modified residue6051Phosphotyrosine; by autocatalysis Potential
Modified residue6111Phosphotyrosine; by autocatalysis Potential
Modified residue8301Phosphotyrosine; by autocatalysis Potential
Modified residue9771Phosphotyrosine; by autocatalysis Potential
Glycosylation3421N-linked (GlcNAc...) Potential
Glycosylation3961N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 513513Missing in isoform 2.
VSP_018473
Alternative sequence514 – 53623IRVRT…ETGDE → MKDSPFQVTKLYWLNEKWDF IAS in isoform 2.
VSP_018474
Alternative sequence834 – 84714GGKIP…APEAI → DLFQTLTLNLCYSA in isoform 2.
VSP_018475
Alternative sequence848 – 1035188Missing in isoform 2.
VSP_018476
Natural variant7031F → S. Ref.4
VAR_042149
Natural variant7101A → V.
Corresponds to variant rs4857276 [ dbSNP | Ensembl ].
VAR_055991

Experimental info

Sequence conflict597 – 5982CK → LR in CAB63775. Ref.1
Sequence conflict7011S → R in CAB63775. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 4D58757D9F4CF178

FASTA1,035116,116
        10         20         30         40         50         60 
MGGCEVREFL LQFGFFLPLL TAWPGDCSHV SNQVVLLDTT TVLGELGWKT YPLNGWDAIT 

        70         80         90        100        110        120 
EMDEHNRPIH TYQVCNVMEP NQNNWLRTNW ISRDAAQKIY VEMKFTLRDC NSIPWVLGTC 

       130        140        150        160        170        180 
KETFNLFYME SDESHGIKFK PNQYTKIDTI AADESFTQMD LGDRILKLNT EIREVGPIER 

       190        200        210        220        230        240 
KGFYLAFQDI GACIALVSVR VFYKKCPFTV RNLAMFPDTI PRVDSSSLVE VRGSCVKSAE 

       250        260        270        280        290        300 
ERDTPKLYCG ADGDWLVPLG RCICSTGYEE IEGSCHACRP GFYKAFAGNT KCSKCPPHSL 

       310        320        330        340        350        360 
TYMEATSVCQ CEKGYFRAEK DPPSMACTGP PSAPRNVVFN INETALILEW SPPSDTGGRK 

       370        380        390        400        410        420 
DLTYSVICKK CGLDTSQCED CGGGLRFIPR HTGLINNSVI VLDFVSHVNY TFEIEAMNGV 

       430        440        450        460        470        480 
SELSFSPKPF TAITVTTDHD APSLIGMMRK DWASQNSIAL SWQAPAFSNG AILDYEIKYY 

       490        500        510        520        530        540 
EKEHEQLTYS STRSKAPSVI ITGLKPATKY VFHIRVRTAT GYSGYSQKFE FETGDEASDM 

       550        560        570        580        590        600 
AAEQGQILVI ATAAVGGFTL LVILTLFFLI TGRCQWYIKA KMKSEEKRRN HLQNGHCKFP 

       610        620        630        640        650        660 
GIKTYIDPDT YEDPSLAVHE FAKEIDPSRI RIERVIGAGE FGEVCSGRLK TPGKREIPVA 

       670        680        690        700        710        720 
IKTLKGGHMD RQRRDFLREA SIMGQFDHPN IIRLEGVVTK SSFPAIGVEA FCPSFLRAGF 

       730        740        750        760        770        780 
LNSIQAPHPV PGGGSLPPRI PAGRPVMIVV EYMENGSLDS FLRKHDGHFT VIQLVGMLRG 

       790        800        810        820        830        840 
IASGMKYLSD MGYVHRDLAA RNILVNSNLV CKVSDFGLSR VLEDDPEAAY TTTGGKIPIR 

       850        860        870        880        890        900 
WTAPEAIAYR KFSSASDAWS YGIVMWEVMS YGERPYWEMS NQDVILSIEE GYRLPAPMGC 

       910        920        930        940        950        960 
PASLHQLMLH CWQKERNHRP KFTDIVSFLD KLIRNPSALH TLVEDILVMP ESPGEVPEYP 

       970        980        990       1000       1010       1020 
LFVTVGDWLD SIKMGQYKNN FVAAGFTTFD LISRMSIEDI RRIGVILIGH QRRIVSSIQT 

      1030 
LRLHMMHIQE KGFHV 

« Hide

Isoform 2 [UniParc].

Checksum: DE9F93BC43795CF0
Show »

FASTA33437,167

References

« Hide 'large scale' references
[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[2]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Differential gene expression of Eph receptors and ephrins in benign human tissues and cancers."
Hafner C., Schmitz G., Meyer S., Bataille F., Hau P., Langmann T., Dietmaier W., Landthaler M., Vogt T.
Clin. Chem. 50:490-499(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-703.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL133666 mRNA. Translation: CAB63775.1.
AC109782 Genomic DNA. No translation available.
AC119745 Genomic DNA. No translation available.
AC107482 Genomic DNA. No translation available.
AC117470 Genomic DNA. No translation available.
AC130510 Genomic DNA. No translation available.
AC117439 Genomic DNA. No translation available.
PIRT43450.
RefSeqNP_001265229.1. NM_001278300.1.
NP_775926.1. NM_173655.3.
UniGeneHs.653244.

3D structure databases

ProteinModelPortalQ9UF33.
SMRQ9UF33. Positions 31-533, 617-953, 959-1025.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid130049. 1 interaction.
STRING9606.ENSP00000374323.

Chemistry

BindingDBQ9UF33.
ChEMBLCHEMBL4526.
GuidetoPHARMACOLOGY1826.

PTM databases

PhosphoSiteQ9UF33.

Polymorphism databases

DMDM97048747.

Proteomic databases

PaxDbQ9UF33.
PRIDEQ9UF33.

Protocols and materials databases

DNASU285220.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000502694; ENSP00000423950; ENSG00000080224.
GeneID285220.
KEGGhsa:285220.
UCSCuc010how.1. human. [Q9UF33-1]

Organism-specific databases

CTD285220.
GeneCardsGC03P096533.
HGNCHGNC:19296. EPHA6.
HPAHPA007397.
HPA058047.
MIM600066. gene.
neXtProtNX_Q9UF33.
PharmGKBPA134866684.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233858.
HOVERGENHBG062180.
InParanoidQ9UF33.
KOK05107.
OrthoDBEOG7VTDM6.
PhylomeDBQ9UF33.

Enzyme and pathway databases

SignaLinkQ9UF33.

Gene expression databases

ArrayExpressQ9UF33.
BgeeQ9UF33.
CleanExHS_EPHA6.
GenevestigatorQ9UF33.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_N_dom.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 2 hits.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSPR00109. TYRKINASE.
SMARTSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 2 hits.
SSF57184. SSF57184. 2 hits.
PROSITEPS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiEPHA6.
GenomeRNAi285220.
NextBio95357.
PROQ9UF33.
SOURCESearch...

Entry information

Entry nameEPHA6_HUMAN
AccessionPrimary (citable) accession number: Q9UF33
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM