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Reviewed, UniProtKB/Swiss-Prot Q9UEY8 (ADDG_HUMAN)

Last modified November 24, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Gamma-adducin
Alternative name(s):
    Adducin-like protein 70
Gene names
Name: ADD3
Synonyms: ADDL
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Subunit structure

Heterodimer of an alpha and a gamma subunit.

Subcellular location

Cytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue specificity

Heart only expresses isoform 1.

Domain

Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Post-translational modification

Sumoylated. Ref.5

Sequence similarities

Belongs to the aldolase class II family. Adducin subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 2 (identifier: Q9UEY8-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q9UEY8-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     576-607: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Gamma-adducin
PRO_0000218536

Regions

Region684 – 70118Interaction with calmodulin Potential

Amino acid modifications

Modified residue121Phosphothreonine
Modified residue421Phosphoserine Ref.8 Ref.9
Modified residue4021Phosphoserine
Modified residue4231Phosphoserine Ref.9
Modified residue4421Phosphoserine Ref.9
Modified residue6731Phosphoserine Ref.9 Ref.6
Modified residue6771Phosphoserine Ref.9 Ref.6
Modified residue6811Phosphoserine Ref.8 Ref.9 Ref.7
Modified residue6831Phosphoserine; by PKC By similarity
Modified residue6931Phosphoserine Ref.9 Ref.6

Natural variations

Alternative sequence576 – 60732Missing in isoform 1.
VSP_000188

Experimental info

Sequence conflict49 – 502FN → SS in BAA23783. Ref.1
Sequence conflict601Q → R in BAA23783. Ref.1
Sequence conflict3621Q → P in AAB17126. Ref.2
Sequence conflict4201V → M in AAB17126. Ref.2
Sequence conflict4211P → L in BAA23783. Ref.1
Sequence conflict426 – 4338KYMAQRQQ → QIHGTRGNK in AAB17126. Ref.2
Sequence conflict4841K → Q in AAB17126. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (Long) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: EB8EAF602A4D7B41

FASTA70679,155
        10         20         30         40         50         60 
MSSDASQGVI TTPPPPSMPH KERYFDRINE NDPEYIRERN MSPDLRQDFN MMEQRKRVTQ 

        70         80         90        100        110        120 
ILQSPAFRED LECLIQEQMK KGHNPTGLLA LQQIADYIMA NSFSGFSSPP LSLGMVTPIN 

       130        140        150        160        170        180 
DLPGADTSSY VKGEKLTRCK LASLYRLVDL FGWAHLANTY ISVRISKEQD HIIIIPRGLS 

       190        200        210        220        230        240 
FSEATASNLV KVNIIGEVVD QGSTNLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV 

       250        260        270        280        290        300 
SSMKCGILPI SQESLLLGDV AYYDYQGSLE EQEERIQLQK VLGPSCKVLV LRNHGVVALG 

       310        320        330        340        350        360 
ETLEEAFHYI FNVQLACEIQ VQALAGAGGV DNLHVLDFQK YKAFTYTVAA SGGGGVNMGS 

       370        380        390        400        410        420 
HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLIREKPRH KSDVEIPATV TAFSFEDDTV 

       430        440        450        460        470        480 
PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN VPEESRNGET SPRTKITWMK AEDSSKVSGG 

       490        500        510        520        530        540 
TPIKIEDPNQ FVPLNTNPNE VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF 

       550        560        570        580        590        600 
EDDDHGPPAP PNPFSHLTEG ELEEYKRTIE RKQQGLEDAE QELLSDDASS VSQIQSQTQS 

       610        620        630        640        650        660 
PQNVPEKLEE NHELFSKSFI SMEVPVMVVN GKDDMHDVED ELAKRVSRLS TSTTIENIEI 

       670        680        690        700 
TIKSPEKIEE VLSPEGSPSK SPSKKKKKFR TPSFLKKNKK KEKVEA

« Hide

Isoform 1 (Short).

Checksum: 28935F90F7C17BD9
Show »

FASTA67475,671

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References

« Hide 'large scale' references
[1]"Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin."
Katagiri T., Ozaki K., Fujiwara T., Shimizu F., Kawai A., Okuno S., Suzuki M., Nakamura Y., Takahashi E., Hirai Y.
Cytogenet. Cell Genet. 74:90-95(1996) [PubMed: 8893809] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning in the gamma quadrant."
Moorthy S., Bennett V.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Genomic organization of the human gamma adducin gene."
Citterio L., Azzani T., Duga S., Bianchi G.
Biochem. Biophys. Res. Commun. 266:110-114(1999) [PubMed: 10581174] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Ovary.
[5]"Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates."
Gocke C.B., Yu H., Kang J.
J. Biol. Chem. 280:5004-5012(2005) [PubMed: 15561718] [Abstract]
Cited for: SUMOYLATION.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673; SER-677 AND SER-693, MASS SPECTROMETRY.
Tissue: Epithelium.
[7]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, MASS SPECTROMETRY.
[8]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-681, MASS SPECTROMETRY.
Tissue: Platelet.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-423; SER-442; SER-673; SER-677; SER-681 AND SER-693, MASS SPECTROMETRY.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, MASS SPECTROMETRY.
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-423; SER-673; SER-677 AND SER-681, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D67031 mRNA. Translation: BAA23783.1.
U37122 mRNA. Translation: AAB17126.1.
Y14372 expand/collapse EMBL AC list , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14383, Y14384 Genomic DNA. Translation: CAB51805.1.
Y14372 expand/collapse EMBL AC list , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14384 Genomic DNA. Translation: CAB51806.1.
BC062559 mRNA. Translation: AAH62559.1.
IPIIPI00004408.
IPI00220754.
PIRJC7164.
RefSeqNP_001112.2.
NP_058432.1.
NP_063968.1.
UniGeneHs.501012

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9UEY8.

PTM databases

PhosphoSiteQ9UEY8.

Proteomic databases

PRIDEQ9UEY8.

Genome annotation databases

EnsemblENST00000356080; ENSP00000348381; ENSG00000148700; Homo sapiens. [Genome view]
GeneID120.
KEGGhsa:120.
UCSCuc001kys.2. human.
uc001kyt.2. human.

Organism-specific databases

CTD120.
GeneCardsGC10P111755.
H-InvDBHIX0025943.
HGNCHGNC:245. ADD3.
HPACAB009797.
MIM601568. gene.
PharmGKBPA24567.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9UEY8.
HOVERGENQ9UEY8.
OMANGETSPR

Enzyme and pathway databases

BioCycCATTLE:505225-MON.

Gene expression databases

ArrayExpressQ9UEY8.
BgeeQ9UEY8.
CleanExHS_ADD3.
GenevestigatorQ9UEY8.
GermOnlineENSG00000148700. Homo sapiens.

Family and domain databases

InterProIPR001303. Aldolase_II/adducin_N.
[Graphical view]
Gene3DG3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit.
PfamPF00596. Aldolase_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio481.
PMAP-CutDBQ9UEY8.
SOURCESearch...

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Entry information

Entry nameADDG_HUMAN
AccessionPrimary (citable) accession number: Q9UEY8
Secondary accession number(s): O43243, Q92773, Q9UEY7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 24, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents