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Q9UEY8 (ADDG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-adducin
Alternative name(s):
Adducin-like protein 70
Gene names
Name:ADD3
Synonyms:ADDL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Subunit structure

Heterodimer of an alpha and a gamma subunit.

Subcellular location

Cytoplasmcytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue specificity

Isoform 1 is ubiquitously expressed. Ref.1

Domain

Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Post-translational modification

Sumoylated. Ref.8

Sequence similarities

Belongs to the aldolase class II family. Adducin subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 2 (identifier: Q9UEY8-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q9UEY8-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     576-607: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.12
Chain2 – 706705Gamma-adducin
PRO_0000218536

Regions

Region684 – 70118Interaction with calmodulin Potential

Amino acid modifications

Modified residue21N-acetylserine Ref.12 Ref.14
Modified residue421Phosphoserine Ref.10 Ref.14
Modified residue641Phosphoserine Ref.11
Modified residue4021Phosphoserine Ref.13
Modified residue4231Phosphoserine Ref.11 Ref.13
Modified residue4421Phosphoserine Ref.11
Modified residue6731Phosphoserine Ref.9 Ref.11 Ref.14
Modified residue6771Phosphoserine Ref.11 Ref.14 Ref.16
Modified residue6811Phosphoserine Ref.11 Ref.13 Ref.16
Modified residue6831Phosphoserine; by PKC By similarity

Natural variations

Alternative sequence576 – 60732Missing in isoform 1.
VSP_000188

Experimental info

Sequence conflict49 – 502FN → SS in BAA23783. Ref.1
Sequence conflict601Q → R in BAA23783. Ref.1
Sequence conflict3621Q → P in AAB17126. Ref.2
Sequence conflict4201V → M in AAB17126. Ref.2
Sequence conflict4211P → L in BAA23783. Ref.1
Sequence conflict426 – 4338KYMAQRQQ → QIHGTRGNK in AAB17126. Ref.2
Sequence conflict4841K → Q in AAB17126. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (Long) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: EB8EAF602A4D7B41

FASTA70679,155
        10         20         30         40         50         60 
MSSDASQGVI TTPPPPSMPH KERYFDRINE NDPEYIRERN MSPDLRQDFN MMEQRKRVTQ 

        70         80         90        100        110        120 
ILQSPAFRED LECLIQEQMK KGHNPTGLLA LQQIADYIMA NSFSGFSSPP LSLGMVTPIN 

       130        140        150        160        170        180 
DLPGADTSSY VKGEKLTRCK LASLYRLVDL FGWAHLANTY ISVRISKEQD HIIIIPRGLS 

       190        200        210        220        230        240 
FSEATASNLV KVNIIGEVVD QGSTNLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV 

       250        260        270        280        290        300 
SSMKCGILPI SQESLLLGDV AYYDYQGSLE EQEERIQLQK VLGPSCKVLV LRNHGVVALG 

       310        320        330        340        350        360 
ETLEEAFHYI FNVQLACEIQ VQALAGAGGV DNLHVLDFQK YKAFTYTVAA SGGGGVNMGS 

       370        380        390        400        410        420 
HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLIREKPRH KSDVEIPATV TAFSFEDDTV 

       430        440        450        460        470        480 
PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN VPEESRNGET SPRTKITWMK AEDSSKVSGG 

       490        500        510        520        530        540 
TPIKIEDPNQ FVPLNTNPNE VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF 

       550        560        570        580        590        600 
EDDDHGPPAP PNPFSHLTEG ELEEYKRTIE RKQQGLEDAE QELLSDDASS VSQIQSQTQS 

       610        620        630        640        650        660 
PQNVPEKLEE NHELFSKSFI SMEVPVMVVN GKDDMHDVED ELAKRVSRLS TSTTIENIEI 

       670        680        690        700 
TIKSPEKIEE VLSPEGSPSK SPSKKKKKFR TPSFLKKNKK KEKVEA 

« Hide

Isoform 1 (Short) [UniParc].

Checksum: 28935F90F7C17BD9
Show »

FASTA67475,671

References

« Hide 'large scale' references
[1]"Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin."
Katagiri T., Ozaki K., Fujiwara T., Shimizu F., Kawai A., Okuno S., Suzuki M., Nakamura Y., Takahashi E., Hirai Y.
Cytogenet. Cell Genet. 74:90-95(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"Cloning in the gamma quadrant."
Moorthy S., Bennett V.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Genomic organization of the human gamma adducin gene."
Citterio L., Azzani T., Duga S., Bianchi G.
Biochem. Biophys. Res. Commun. 266:110-114(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Endometrium.
[5]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Ovary.
[8]"Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates."
Gocke C.B., Yu H., Kang J.
J. Biol. Chem. 280:5004-5012(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION.
[9]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64; SER-423; SER-442; SER-673; SER-677 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[13]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-423 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-673 AND SER-677, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D67031 mRNA. Translation: BAA23783.1.
U37122 mRNA. Translation: AAB17126.1.
Y14372 expand/collapse EMBL AC list , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14383, Y14384 Genomic DNA. Translation: CAB51805.1.
Y14372 expand/collapse EMBL AC list , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14384 Genomic DNA. Translation: CAB51806.1.
BX647403 mRNA. Translation: CAI46048.1.
AL590628 Genomic DNA. Translation: CAH71739.1.
CH471066 Genomic DNA. Translation: EAW49573.1.
CH471066 Genomic DNA. Translation: EAW49574.1.
BC062559 mRNA. Translation: AAH62559.1.
CCDSCCDS7561.1. [Q9UEY8-1]
CCDS7562.1. [Q9UEY8-2]
PIRJC7164.
RefSeqNP_001112.2. NM_001121.2. [Q9UEY8-2]
NP_058432.1. NM_016824.3. [Q9UEY8-1]
NP_063968.1. NM_019903.3. [Q9UEY8-2]
XP_005269586.1. XM_005269529.1. [Q9UEY8-1]
XP_005269587.1. XM_005269530.1. [Q9UEY8-1]
XP_005269588.1. XM_005269531.2. [Q9UEY8-1]
XP_005269590.1. XM_005269533.1. [Q9UEY8-1]
XP_005269591.1. XM_005269534.2. [Q9UEY8-1]
XP_006717689.1. XM_006717626.1. [Q9UEY8-1]
XP_006717690.1. XM_006717627.1. [Q9UEY8-1]
XP_006717691.1. XM_006717628.1. [Q9UEY8-1]
UniGeneHs.501012.

3D structure databases

ProteinModelPortalQ9UEY8.
SMRQ9UEY8. Positions 153-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106633. 5 interactions.
IntActQ9UEY8. 5 interactions.
STRING9606.ENSP00000348381.

PTM databases

PhosphoSiteQ9UEY8.

Polymorphism databases

DMDM12643881.

Proteomic databases

MaxQBQ9UEY8.
PaxDbQ9UEY8.
PRIDEQ9UEY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000277900; ENSP00000277900; ENSG00000148700. [Q9UEY8-2]
ENST00000356080; ENSP00000348381; ENSG00000148700. [Q9UEY8-1]
ENST00000360162; ENSP00000353286; ENSG00000148700. [Q9UEY8-2]
GeneID120.
KEGGhsa:120.
UCSCuc001kys.4. human. [Q9UEY8-2]
uc001kyt.4. human. [Q9UEY8-1]

Organism-specific databases

CTD120.
GeneCardsGC10P111755.
H-InvDBHIX0170444.
HGNCHGNC:245. ADD3.
HPACAB009797.
HPA035696.
MIM601568. gene.
neXtProtNX_Q9UEY8.
Orphanet210141. Inherited congenital spastic tetraplegia.
PharmGKBPA24567.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0235.
HOGENOMHOG000116349.
HOVERGENHBG004180.
InParanoidQ9UEY8.
OMAVNVPEES.
OrthoDBEOG7HF1HR.
PhylomeDBQ9UEY8.
TreeFamTF313003.

Gene expression databases

ArrayExpressQ9UEY8.
BgeeQ9UEY8.
CleanExHS_ADD3.
GenevestigatorQ9UEY8.

Family and domain databases

Gene3D3.40.225.10. 1 hit.
InterProIPR027772. ADD3.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERPTHR10672:SF5. PTHR10672:SF5. 1 hit.
PfamPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMSSF53639. SSF53639. 1 hit.
ProtoNetSearch...

Other

ChiTaRSADD3. human.
GeneWikiADD3.
GenomeRNAi120.
NextBio481.
PMAP-CutDBQ9UEY8.
PROQ9UEY8.
SOURCESearch...

Entry information

Entry nameADDG_HUMAN
AccessionPrimary (citable) accession number: Q9UEY8
Secondary accession number(s): D3DRA8 expand/collapse secondary AC list , O43243, Q5VU09, Q92773, Q9UEY7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM