Q9UEY8 (ADDG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-adducin

Alternative name(s):
Adducin-like protein 70

Gene names

Name:	ADD3
Synonyms:	ADDL

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Subunit structure	Heterodimer of an alpha and a gamma subunit.
Subcellular location	Cytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue specificity	Heart only expresses isoform 1.
Domain	Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Post-translational modification	Sumoylated. Ref.8
Sequence similarities	Belongs to the aldolase class II family. Adducin subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Ligand	Actin-binding Calmodulin-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro structural constituent of cytoskeleton Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 2 (identifier: Q9UEY8-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9UEY8-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     576-607: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Gamma-adducin		PRO_0000218536
Regions
	Region	684 – 701	18	Interaction with calmodulin Potential
Amino acid modifications
	Modified residue	12	1	Phosphothreonine Ref.13
	Modified residue	42	1	Phosphoserine Ref.11 Ref.12
	Modified residue	402	1	Phosphoserine Ref.14
	Modified residue	423	1	Phosphoserine Ref.12 Ref.14
	Modified residue	442	1	Phosphoserine Ref.12
	Modified residue	673	1	Phosphoserine Ref.9 Ref.12 Ref.14
	Modified residue	677	1	Phosphoserine Ref.9 Ref.12 Ref.14
	Modified residue	681	1	Phosphoserine Ref.10 Ref.11 Ref.12 Ref.14
	Modified residue	683	1	Phosphoserine; by PKC By similarity
	Modified residue	693	1	Phosphoserine Ref.9 Ref.12
Natural variations
	Alternative sequence	576 – 607	32	Missing in isoform 1.		VSP_000188
Experimental info
	Sequence conflict	49 – 50	2	FN → SS in BAA23783. Ref.1
	Sequence conflict	60	1	Q → R in BAA23783. Ref.1
	Sequence conflict	362	1	Q → P in AAB17126. Ref.2
	Sequence conflict	420	1	V → M in AAB17126. Ref.2
	Sequence conflict	421	1	P → L in BAA23783. Ref.1
	Sequence conflict	426 – 433	8	KYMAQRQQ → QIHGTRGNK in AAB17126. Ref.2
	Sequence conflict	484	1	K → Q in AAB17126. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 (Long) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: EB8EAF602A4D7B41	FASTA	706	79,155
	10 20 30 40 50 60 MSSDASQGVI TTPPPPSMPH KERYFDRINE NDPEYIRERN MSPDLRQDFN MMEQRKRVTQ 70 80 90 100 110 120 ILQSPAFRED LECLIQEQMK KGHNPTGLLA LQQIADYIMA NSFSGFSSPP LSLGMVTPIN 130 140 150 160 170 180 DLPGADTSSY VKGEKLTRCK LASLYRLVDL FGWAHLANTY ISVRISKEQD HIIIIPRGLS 190 200 210 220 230 240 FSEATASNLV KVNIIGEVVD QGSTNLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV 250 260 270 280 290 300 SSMKCGILPI SQESLLLGDV AYYDYQGSLE EQEERIQLQK VLGPSCKVLV LRNHGVVALG 310 320 330 340 350 360 ETLEEAFHYI FNVQLACEIQ VQALAGAGGV DNLHVLDFQK YKAFTYTVAA SGGGGVNMGS 370 380 390 400 410 420 HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLIREKPRH KSDVEIPATV TAFSFEDDTV 430 440 450 460 470 480 PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN VPEESRNGET SPRTKITWMK AEDSSKVSGG 490 500 510 520 530 540 TPIKIEDPNQ FVPLNTNPNE VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF 550 560 570 580 590 600 EDDDHGPPAP PNPFSHLTEG ELEEYKRTIE RKQQGLEDAE QELLSDDASS VSQIQSQTQS 610 620 630 640 650 660 PQNVPEKLEE NHELFSKSFI SMEVPVMVVN GKDDMHDVED ELAKRVSRLS TSTTIENIEI 670 680 690 700 TIKSPEKIEE VLSPEGSPSK SPSKKKKKFR TPSFLKKNKK KEKVEA
	Isoform 1 (Short) [UniParc]. Checksum: 28935F90F7C17BD9	FASTA	674	75,671
	10 20 30 40 50 60 MSSDASQGVI TTPPPPSMPH KERYFDRINE NDPEYIRERN MSPDLRQDFN MMEQRKRVTQ 70 80 90 100 110 120 ILQSPAFRED LECLIQEQMK KGHNPTGLLA LQQIADYIMA NSFSGFSSPP LSLGMVTPIN 130 140 150 160 170 180 DLPGADTSSY VKGEKLTRCK LASLYRLVDL FGWAHLANTY ISVRISKEQD HIIIIPRGLS 190 200 210 220 230 240 FSEATASNLV KVNIIGEVVD QGSTNLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV 250 260 270 280 290 300 SSMKCGILPI SQESLLLGDV AYYDYQGSLE EQEERIQLQK VLGPSCKVLV LRNHGVVALG 310 320 330 340 350 360 ETLEEAFHYI FNVQLACEIQ VQALAGAGGV DNLHVLDFQK YKAFTYTVAA SGGGGVNMGS 370 380 390 400 410 420 HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLIREKPRH KSDVEIPATV TAFSFEDDTV 430 440 450 460 470 480 PLSPLKYMAQ RQQREKTRWL NSPNTYMKVN VPEESRNGET SPRTKITWMK AEDSSKVSGG 490 500 510 520 530 540 TPIKIEDPNQ FVPLNTNPNE VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF 550 560 570 580 590 600 EDDDHGPPAP PNPFSHLTEG ELEEYKRTIE RKQQGLEENH ELFSKSFISM EVPVMVVNGK 610 620 630 640 650 660 DDMHDVEDEL AKRVSRLSTS TTIENIEITI KSPEKIEEVL SPEGSPSKSP SKKKKKFRTP 670 SFLKKNKKKE KVEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin." Katagiri T., Ozaki K., Fujiwara T., Shimizu F., Kawai A., Okuno S., Suzuki M., Nakamura Y., Takahashi E., Hirai Y. Cytogenet. Cell Genet. 74:90-95(1996) [PubMed: 8893809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning in the gamma quadrant." Moorthy S., Bennett V. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Genomic organization of the human gamma adducin gene." Citterio L., Azzani T., Duga S., Bianchi G. Biochem. Biophys. Res. Commun. 266:110-114(1999) [PubMed: 10581174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Endometrium.
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary.
[8]	"Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates." Gocke C.B., Yu H., Kang J. J. Biol. Chem. 280:5004-5012(2005) [PubMed: 15561718] [Abstract] Cited for: SUMOYLATION.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673; SER-677 AND SER-693, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-681, MASS SPECTROMETRY. Tissue: Platelet.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-423; SER-442; SER-673; SER-677; SER-681 AND SER-693, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-423; SER-673; SER-677 AND SER-681, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	D67031 mRNA. Translation: BAA23783.1. U37122 mRNA. Translation: AAB17126.1. Y14372 , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14383, Y14384 Genomic DNA. Translation: CAB51805.1. Y14372 , Y14373, Y14374, Y14375, Y14376, Y14377, Y14378, Y14379, Y14380, Y14381, Y14382, Y14384 Genomic DNA. Translation: CAB51806.1. BX647403 mRNA. Translation: CAI46048.1. AL590628 Genomic DNA. Translation: CAH71739.1. CH471066 Genomic DNA. Translation: EAW49573.1. CH471066 Genomic DNA. Translation: EAW49574.1. BC062559 mRNA. Translation: AAH62559.1.
IPI	IPI00004408. IPI00220754.
PIR	JC7164.
RefSeq	NP_001112.2. NM_001121.2. NP_058432.1. NM_016824.3. NP_063968.1. NM_019903.3.
UniGene	Hs.501012.
3D structure databases
ProteinModelPortal	Q9UEY8.
SMR	Q9UEY8. Positions 124-391.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UEY8. 1 interaction.
STRING	Q9UEY8.
PTM databases
PhosphoSite	Q9UEY8.
Polymorphism databases
DMDM	12643881.
Proteomic databases
PRIDE	Q9UEY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000356080; ENSP00000348381; ENSG00000148700.
GeneID	120.
KEGG	hsa:120.
UCSC	uc001kys.2. human. uc001kyt.2. human.
Organism-specific databases
CTD	120.
GeneCards	GC10P111755.
H-InvDB	HIX0025943.
HGNC	HGNC:245. ADD3.
HPA	CAB009797.
MIM	601568. gene.
neXtProt	NX_Q9UEY8.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10779.
GeneTree	ENSGT00390000016462.
HOGENOM	HBG388064.
HOVERGEN	HBG004180.
InParanoid	Q9UEY8.
OMA	IREQNRY.
PhylomeDB	Q9UEY8.
Gene expression databases
ArrayExpress	Q9UEY8.
Bgee	Q9UEY8.
CleanEx	HS_ADD3.
Genevestigator	Q9UEY8.
GermOnline	ENSG00000148700. Homo sapiens.
Family and domain databases
InterPro	IPR001303. Aldolase_II/adducin_N. [Graphical view]
Gene3D	G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit.
Pfam	PF00596. Aldolase_II. 1 hit. [Graphical view]
SMART	SM01007. Aldolase_II. 1 hit. [Graphical view]
SUPFAM	SSF53639. Aldolase_II_N. 1 hit.
ProtoNet	Search...
Other
NextBio	481.
PMAP-CutDB	Q9UEY8.
SOURCE	Search...

Entry information

Entry name

ADDG_HUMAN

Accession

Primary (citable) accession number: Q9UEY8
Secondary accession number(s): D3DRA8 , O43243, Q5VU09, Q92773, Q9UEY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.