Reviewed,
UniProtKB/Swiss-Prot Q9UEY8 (ADDG_HUMAN)
Last modified
November 24, 2009.
Version 78.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Gamma-adducin Alternative name(s): Adducin-like protein 70 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 706 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
| Subunit structure | Heterodimer of an alpha and a gamma subunit. |
| Subcellular location | Cytoplasm › cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Tissue specificity | Heart only expresses isoform 1. |
| Domain | Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. |
| Post-translational modification | Sumoylated. Ref.5 |
| Sequence similarities | Belongs to the aldolase class II family. Adducin subfamily. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Cytoplasm Cytoskeleton Membrane |
| Coding sequence diversity | Alternative splicing |
| Ligand | Actin-binding Calmodulin-binding |
| PTM | Phosphoprotein Ubl conjugation |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Cellular component | cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | actin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin bindingInferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: InterPro structural constituent of cytoskeleton Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 2 (identifier: Q9UEY8-1) Also known as: Long; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 1 (identifier: Q9UEY8-2) Also known as: Short; The sequence of this isoform differs from the canonical sequence as follows: 576-607: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 706 | 706 | Gamma-adducin | PRO_0000218536 | |||||
Regions | |||||||||
| Region | 684 – 701 | 18 | Interaction with calmodulin Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 12 | 1 | Phosphothreonine | ||||||
| Modified residue | 42 | 1 | Phosphoserine Ref.8 Ref.9 | ||||||
| Modified residue | 402 | 1 | Phosphoserine | ||||||
| Modified residue | 423 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 442 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 673 | 1 | Phosphoserine Ref.9 Ref.6 | ||||||
| Modified residue | 677 | 1 | Phosphoserine Ref.9 Ref.6 | ||||||
| Modified residue | 681 | 1 | Phosphoserine Ref.8 Ref.9 Ref.7 | ||||||
| Modified residue | 683 | 1 | Phosphoserine; by PKC By similarity | ||||||
| Modified residue | 693 | 1 | Phosphoserine Ref.9 Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 576 – 607 | 32 | Missing in isoform 1. | VSP_000188 | |||||
Experimental info | |||||||||
| Sequence conflict | 49 – 50 | 2 | FN → SS in BAA23783. Ref.1 | ||||||
| Sequence conflict | 60 | 1 | Q → R in BAA23783. Ref.1 | ||||||
| Sequence conflict | 362 | 1 | Q → P in AAB17126. Ref.2 | ||||||
| Sequence conflict | 420 | 1 | V → M in AAB17126. Ref.2 | ||||||
| Sequence conflict | 421 | 1 | P → L in BAA23783. Ref.1 | ||||||
| Sequence conflict | 426 – 433 | 8 | KYMAQRQQ → QIHGTRGNK in AAB17126. Ref.2 | ||||||
| Sequence conflict | 484 | 1 | K → Q in AAB17126. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning, expression and chromosome mapping of adducin-like 70 (ADDL), a human cDNA highly homologous to human erythrocyte adducin." Katagiri T., Ozaki K., Fujiwara T., Shimizu F., Kawai A., Okuno S., Suzuki M., Nakamura Y., Takahashi E., Hirai Y. Cytogenet. Cell Genet. 74:90-95(1996) [PubMed: 8893809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Cloning in the gamma quadrant." Moorthy S., Bennett V. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [3] | "Genomic organization of the human gamma adducin gene." Citterio L., Azzani T., Duga S., Bianchi G. Biochem. Biophys. Res. Commun. 266:110-114(1999) [PubMed: 10581174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary. |
| [5] | "Systematic identification and analysis of mammalian small ubiquitin-like modifier substrates." Gocke C.B., Yu H., Kang J. J. Biol. Chem. 280:5004-5012(2005) [PubMed: 15561718] [Abstract] Cited for: SUMOYLATION. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673; SER-677 AND SER-693, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-681, MASS SPECTROMETRY. |
| [8] | "Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-681, MASS SPECTROMETRY. Tissue: Platelet. |
| [9] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-423; SER-442; SER-673; SER-677; SER-681 AND SER-693, MASS SPECTROMETRY. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [11] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-12, MASS SPECTROMETRY. |
| [12] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-402; SER-423; SER-673; SER-677 AND SER-681, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D67031 mRNA. Translation: BAA23783.1. U37122 mRNA. Translation: AAB17126.1. Y14372 Y14384 Genomic DNA. Translation: CAB51805.1. Y14372 Y14384 Genomic DNA. Translation: CAB51806.1. BC062559 mRNA. Translation: AAH62559.1. | |
| IPI | IPI00004408. IPI00220754. |
| PIR | JC7164. |
| RefSeq | NP_001112.2. NP_058432.1. NP_063968.1. |
| UniGene | Hs.501012 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9UEY8. |
PTM databases | |
| PhosphoSite | Q9UEY8. |
Proteomic databases | |
| PRIDE | Q9UEY8. |
Genome annotation databases | |
| Ensembl | ENST00000356080; ENSP00000348381; ENSG00000148700; Homo sapiens. [Genome view] |
| GeneID | 120. |
| KEGG | hsa:120. |
| UCSC | uc001kys.2. human. uc001kyt.2. human. |
Organism-specific databases | |
| CTD | 120. |
| GeneCards | GC10P111755. |
| H-InvDB | HIX0025943. |
| HGNC | HGNC:245. ADD3. |
| HPA | CAB009797. |
| MIM | 601568. gene. |
| PharmGKB | PA24567. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9UEY8. |
| HOVERGEN | Q9UEY8. |
| OMA | NGETSPR |
Enzyme and pathway databases | |
| BioCyc | CATTLE:505225-MON. |
Gene expression databases | |
| ArrayExpress | Q9UEY8. |
| Bgee | Q9UEY8. |
| CleanEx | HS_ADD3. |
| Genevestigator | Q9UEY8. |
| GermOnline | ENSG00000148700. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001303. Aldolase_II/adducin_N. [Graphical view] |
| Gene3D | G3DSA:3.40.225.10. Aldolase_II/adducin_N. 1 hit. |
| Pfam | PF00596. Aldolase_II. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 481. |
| PMAP-CutDB | Q9UEY8. |
| SOURCE | Search... |
Entry information
| Entry name | ADDG_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9UEY8 Secondary accession number(s): O43243, Q92773, Q9UEY7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


